LOCUS       Exported               10565 bp ds-DNA     linear   SYN 12-JAN-2024
DEFINITION  V3: TRE-mClover3-linker-BsmBI-2A-neo-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
       61 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
      121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
      181 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
      481 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
      541 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
      601 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
      661 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
      721 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
      781 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
      841 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
      901 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
      961 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     1021 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     1081 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1141 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1261 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     1321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1441 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1501 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2401 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2461 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2521 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2581 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2641 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2701 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2761 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2821 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2881 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2941 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3001 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3061 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3121 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3181 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3241 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3301 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3361 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     3481 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     3541 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     3601 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     3661 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     3721 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3781 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3841 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3901 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3961 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     4021 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4081 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4141 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4201 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4261 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     4321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     4921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     4981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     5041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     5101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     5581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     5641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     5701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     5761 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     5821 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     5881 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     5941 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     6001 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     6061 ttggttaatt acagcagaga tccagtttgg ttaatcgagt ttactcccta tcagtgatag
     6121 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct
     6181 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     6241 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag
     6301 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta
     6361 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt
     6421 ggcaccgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     6481 gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg agggcgatgc
     6541 caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     6601 gcccaccctc gtgaccacct tcggctacgg cgtggcctgc ttcagccgct accccgacca
     6661 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     6721 catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt tcgagggcga
     6781 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     6841 ggggcacaag ctggagtaca acttcaacag ccactacgtc tatatcacgg ccgacaagca
     6901 gaagaactgc atcaaggcta acttcaagat ccgccacaac gttgaggacg gcagcgtgca
     6961 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     7021 caaccactac ctgagccatc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     7081 catggtcctg ctggagttcg tgaccgccgc cgggattaca catggcatgg acgagctgta
     7141 caagggatcc ggaagtggct caagcggagg agacggacgt ctctggatcc ggcgcaacaa
     7201 acttctctct gctgaaacaa gccggagatg tcgaagagaa tcctggaccg ggatcggcca
     7261 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     7321 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     7381 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     7441 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7501 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     7561 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     7621 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7681 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7741 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     7801 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     7861 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     7921 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     7981 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     8041 agttcttctg agaattcgct agctaggtct tgaaaggagt gggaattggc tccggtgccc
     8101 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     8161 attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     8221 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     8281 acgttctttt tcgcaacggg tttgccgcca gaacacagga ccggttctag agcgctgcca
     8341 ccatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     8401 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     8461 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     8521 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     8581 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     8641 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     8701 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     8761 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     8821 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     8881 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     8941 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     9001 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     9061 attttgacct tgacatgctc cccgggtaac taagtaagga tcgatccaag atatcgtatt
     9121 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     9181 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     9241 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     9301 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     9361 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     9421 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     9481 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     9541 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     9601 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     9661 ccgcctgttt cgcctcggcg tccggactag aggtaccttt aagaccaatg acttacaagg
     9721 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     9781 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     9841 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9901 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9961 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
    10021 gcctcgactg taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
    10081 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
    10141 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
    10201 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
    10261 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
    10321 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    10381 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    10441 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    10501 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    10561 gccat
//
