LOCUS       Exported               10559 bp ds-DNA     linear   SYN 12-JAN-2024
DEFINITION  V4: TRE-mRuby3-linker-BsmBI-2A-neo-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10559
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
       61 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
      121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
      181 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
      361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
      481 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
      541 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
      601 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
      661 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
      721 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
      781 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
      841 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
      901 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
      961 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     1021 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     1081 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1141 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1261 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     1321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1441 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1501 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2401 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2461 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2521 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2581 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2641 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2701 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2761 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2821 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2881 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2941 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3001 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3061 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3121 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3181 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3241 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3301 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3361 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     3481 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     3541 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     3601 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     3661 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     3721 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     3781 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     3841 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3901 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3961 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     4021 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4081 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4141 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4201 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4261 gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta
     4321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     4501 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     4561 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     4621 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     4681 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     4741 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     4801 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     4861 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     4921 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     4981 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     5041 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     5101 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     5161 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     5221 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     5281 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     5341 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     5401 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     5461 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     5521 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     5581 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     5641 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     5701 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     5761 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     5821 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
     5881 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     5941 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     6001 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
     6061 ttggttaatt acagcagaga tccagtttgg ttaatcgagt ttactcccta tcagtgatag
     6121 agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag tttactccct
     6181 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     6241 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc agtgatagag
     6301 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg taggcgtgta
     6361 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagaatt
     6421 ggcaccgatg gtgtctaagg gcgaagagct gatcaaggaa aatatgcgta tgaaggtggt
     6481 catggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag aaggcagacc
     6541 gtacgaggga gtgcaaacca tgaggatcaa agtcatcgag ggaggacccc tgccatttgc
     6601 ctttgacatt cttgccacgt cgttcatgta tggcagccgt acctttatca agtacccggc
     6661 cgacatccct gatttcttta aacagtcctt tcctgagggt tttacttggg aaagagttac
     6721 gagatacgaa gatggtggag tcgtcaccgt cacgcaggac accagccttg aggatggcga
     6781 gctcgtctac aacgtcaagg tcagaggggt aaactttccc tccaatggtc ccgtgatgca
     6841 gaagaagacc aagggttggg agcctaatac agagatgatg tatccagcag atggtggtct
     6901 gagaggatac actgacatcg cactgaaagt tgatggtggt ggccatctgc actgcaactt
     6961 cgtgacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg gtgtccatgc
     7021 cgttgatcac cgcctggaaa ggatcgagga gagtgacaat gaaacctacg tagtgcaaag
     7081 agaagtggca gttgccaaat acagcaacct tggtggtggc atggacgagc tgtacaaggg
     7141 atccggaagt ggctcaagcg gaggagacgg acgtctctgg atccggcgca acaaacttct
     7201 ctctgctgaa acaagccgga gatgtcgaag agaatcctgg accgggatcg gccattgaac
     7261 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7321 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7381 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     7441 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7501 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7561 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7621 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7681 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7741 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     7801 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7861 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7921 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7981 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     8041 tctgagaatt cgctagctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     8101 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat
     8161 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     8221 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     8281 tttttcgcaa cgggtttgcc gccagaacac aggaccggtt ctagagcgct gccaccatgt
     8341 ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta
     8401 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     8461 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     8521 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     8581 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     8641 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     8701 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     8761 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     8821 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     8881 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     8941 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
     9001 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     9061 accttgacat gctccccggg taactaagta aggatcgatc caagatatcg tattcttaac
     9121 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     9181 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     9241 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     9301 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     9361 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     9421 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     9481 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     9541 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     9601 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     9661 gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac aaggcagctg
     9721 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9781 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     9841 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9901 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9961 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
    10021 actgtaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
    10081 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    10141 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    10201 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
    10261 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
    10321 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    10381 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    10441 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    10501 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccat
//
