LOCUS       Exported                7230 bp ds-DNA     circular SYN 12-JAN-2024
DEFINITION  Expression of SomArchon under Syn promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-Syn-Cepheid1b-ST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7230)
  TITLE     Population imaging of neural activity in awake behaving mice in 
            multiple brain regions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        341..788
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      801..806
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             807..3335
                     /codon_start=1
                     /label=Cepheid-ST
                     /translation="MADVETETGMIAQWIVFAIMAAAAIAFGVAVHFRPSELKSAYYIN
                     IAICTIAATAYYAMAVNYQDLTMMVSKGEAVIKEFMRFKVHMEGSVNGHEFEIEGEGEG
                     RPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFTWE
                     RVMNFEDGGSVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKTTMGWEASTERLYPE
                     DGVLKGDIKMALRLKDGGRYIADFKTTYKAKKPVQMPGAYNVDRKLDIVSHNEDYTVVE
                     QYEASVGRHSTGGMDELYKNGERQVVYARYICWVLTTPLLLLDLIVMTKMGGVMISWVI
                     GADIFMIVFGILGAFEDEHKFKWVYFIAGCVMQAVLTYGMYNATWKDDLKKSPEYHSSY
                     VSLLVFLSILWVFYPVVWAFGSGSGVLSVDNEAILMGILDVLAKPLFGMGCLIAHETIF
                     KTGAADRPVVAVSKAAAASKSRITSEGEYIPLDQIDINVGGKSRITSEGEYIPLDQIDI
                     NVGGKSRITSEGEYIPLDQIDINVGGNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGR
                     PYEGFQTAKLKVTKGGPLPFAWDILSPHFTFGSKAYVKHPADIPDYFKLSFPEGFKWER
                     VMNYEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPED
                     GALKGKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYKQSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVI
                     DMRSMSSIDSFISCATDFPEATRFFCYENEV"
     CDS             1011..1706
                     /codon_start=1
                     /label=mScarlet2
                     /translation="MVSKGEAVIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFTWERVMNFEDGG
                     SVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKTTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYIADFKTTYKAKKPVQMPGAYNVDRKLDIVSHNEDYTVVEQYEASVGRH
                     STGGMDELYK"
     CDS             2238..2297
                     /codon_start=1
                     /label=TS
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             2304..2363
                     /codon_start=1
                     /label=TS
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             2370..2429
                     /codon_start=1
                     /label=TS
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             2436..3119
                     /codon_start=1
                     /label=Dark mO2
                     /translation="NMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGFQTAKLKV
                     TKGGPLPFAWDILSPHFTFGSKAYVKHPADIPDYFKLSFPEGFKWERVMNYEDGGVVTV
                     TQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGKIKMRLK
                     LKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERAEGRHSTGG
                     MDELYK"
     CDS             3120..3314
                     /codon_start=1
                     /label=Kv2.1
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     CDS             3336..3338
                     /codon_start=1
                     /label=Stop coden
                     /translation=""
     misc_feature    3363..3951
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3834..3845)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3971..4447
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4493..4633
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4493..4622
                     /label=AAV2 ITR
     rep_origin      4708..5163
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5445..5549
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5550..6410
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6581..7169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
      181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
      301 aatggttaat taatctagac tgcagagggc cctgcgtatg agtgcaagtg ggttttagga
      361 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
      421 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
      481 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
      541 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc
      601 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg
      661 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg
      721 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga
      781 gagcgcagtc gagaggatcc gccaccatgg ctgacgtgga aaccgagacc ggcatgattg
      841 cacagtggat tgtctttgct attatggctg ctgctgctat tgcttttgga gtggctgtgc
      901 actttcggcc ttcagagctg aagagcgcat actatatcaa cattgccatc tgcactatcg
      961 ccgctaccgc ttactatgca atggccgtga actaccagga cctgacaatg atggtgagca
     1021 agggcgaggc agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccgtga
     1081 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1141 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc
     1201 ctcagttcat gtacggctcc agggccttca ccaagcaccc cgccgacatc cccgactact
     1261 ataagcagtc cttccccgag ggcttcacct gggagcgcgt gatgaacttc gaggacggcg
     1321 gctccgtgac cgtgacccag gacacctccc tggaggacgg caccctgatc tacaaggtga
     1381 agctccgcgg caccaacttc cctcctgacg gccccgtaat gcagaagaca acaatgggct
     1441 gggaagcgtc caccgagcgg ttgtaccccg aggacggcgt gctgaagggc gacattaaga
     1501 tggccctgcg cctgaaggac ggcggccgct acatcgcgga cttcaagacc acctacaagg
     1561 ccaagaagcc cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg gacatcgtct
     1621 cccacaacga ggactacacc gtggtggaac agtacgaagc ctccgtgggc cgccactcca
     1681 ccggcggcat ggacgagctg tacaagaatg gtgaaaggca ggtggtctac gcaagatata
     1741 tttgttgggt gctgaccaca ccactgctcc tgctcgatct catcgtcatg accaagatgg
     1801 gcggagtgat gatttcttgg gtcatcggcg cagacatttt catgatcgtg tttggtattc
     1861 tgggcgcctt cgaggatgaa cacaagttca aatgggtgta ctttatcgct ggatgtgtga
     1921 tgcaggcagt cctgacatac gggatgtata acgccacttg gaaagacgat ctgaagaaaa
     1981 gccccgagta ccatagctcc tatgtcagtc tgctcgtctt cctgtcaatc ctctgggtgt
     2041 tttatcctgt cgtgtgggct ttcgggtctg gtagtggcgt gctgtccgtc gacaatgagg
     2101 ccattctcat gggaatcctg gatgtgctcg ctaagccact gtttggaatg gggtgcctca
     2161 ttgcccatga gactatcttc aagaccggtg ccgccgaccg cccggtagta gcagtgagca
     2221 aggcggccgc cgctagcaaa tcaaggatca cctctgaggg cgagtatatc cctctggatc
     2281 agatcgacat taacgtcggt ggaaaatccc gcataacctc tgagggcgag tacatccctc
     2341 tggatcaaat cgatataaac gtggggggaa aaagtcgaat cacttctgag ggcgagtata
     2401 tacccctcga tcagattgac atcaatgtgg ggggcaacat ggccatcatc aaggagttca
     2461 tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     2521 agggcgaggg ccgcccctac gagggctttc agaccgctaa gctgaaggtg accaagggtg
     2581 gccccctgcc cttcgcctgg gacatcctgt cccctcattt cacctttggc tccaaggcct
     2641 acgtgaagca ccccgccgac atccccgact acttcaagct gtccttcccc gagggcttca
     2701 agtgggagcg cgtgatgaac tacgaggacg gcggcgtggt gaccgtgacc caggactcct
     2761 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     2821 acggccccgt gatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     2881 ccgaggacgg tgccctgaag ggcaagatca agatgaggct gaagctgaag gacggcggcc
     2941 actacacctc cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     3001 cctacatcgt cgacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     3061 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagc
     3121 agagccagcc tatcctgaac accaaggaga tggcccctca gagcaagcct cctgaggagc
     3181 tggagatgag cagcatgcct agccctgtgg cccctctgcc tgccaggacc gagggcgtga
     3241 tcgacatgag gagcatgagc agcatcgaca gcttcatcag ctgcgccacc gacttccctg
     3301 aggccaccag gttcttctgc tatgaaaatg aagtgtaaga attcgatatc aagcttatcg
     3361 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3721 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3781 tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     3961 ggctcgagac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     4021 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     4081 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     4141 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     4201 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     4261 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     4321 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     4381 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
     4441 tgtccttctc gagctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc
     4501 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4561 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4621 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4681 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4741 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
     4801 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4861 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4921 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4981 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
     5041 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
     5101 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5161 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5221 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5281 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5341 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5401 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5461 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5521 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5581 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5641 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5701 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5761 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5821 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5881 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5941 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6001 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6061 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6121 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6181 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6241 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6301 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6361 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6421 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6481 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6541 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6601 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6661 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6721 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6781 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6841 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6901 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6961 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7021 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7081 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7141 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7201 tggccttttg ctggcctttt gctcacatgt
//
