LOCUS       EGFP-CCNE1_[pBABE.puro] 7125 bp ds-DNA     circular     18-JUN-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     LTR             8..479
                     /label="LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    541..541
                     /label="splice donor mutation"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_feature    542..741
                     /label="MMLV Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             934..1350
                     /label="gag (truncated)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    1391..2107
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1391..3352
                     /label="Translation 1391-3352"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA*"
     misc_feature    2108..2122
                     /label="Linker"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2123..3352
                     /label="CCNE1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        3369..3698
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      3549..3684
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3708..4307
                     /label="PuroR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     LTR             4406..4999
                     /label="LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(5371..5959)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(6137..6928)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(6929..6997)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(6998..7102)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 CTGCAGCCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
       61 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT
      121 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG
      181 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC
      241 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA
      301 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC
      361 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC
      421 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATTT
      481 GGGGGCTCGT CCGGGATCGG GAGACCCCTG CCCAGGGACC ACCGACCCAC CACCGGGAGG
      541 CAAGCTGGCC AGCAACTTAT CTGTGTCTGT CCGATTGTCT AGTGTCTATG ACTGATTTTA
      601 TGCGCCTGCG TCGGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA
      661 CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC
      721 GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG
      781 TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT
      841 CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT
      901 CTGACTGTGT TTCTGTATTT GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT
      961 TTGACCTTAG ATCACTGGAA AGATGTCGAG CGGCTCGCTC ACAACCAGTC GGTAGATGTC
     1021 AAGAAGAGAC GTTGGGTTAC CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG
     1081 CCGCGAGACG GCACCTTTAA CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA
     1141 CCTGGCCCGC ATGGACACCC AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT
     1201 TTTGACCCCC CTCCCTGGGT CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCTT
     1261 CCATCCGCGC CGTCTCTCCC CCTTGAACCT CCTCTTTCGA CCCCGCCTCA ATCCTCCCTT
     1321 TATCCAGCCC TCACTCCTTC TCTAGGCGCC GGCCGGATCC CAGTGTGGTG GTACGCTACC
     1381 GGTCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT
     1441 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA
     1501 TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC
     1561 CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA
     1621 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG
     1681 CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG
     1741 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT
     1801 CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA
     1861 GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT
     1921 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC
     1981 CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA
     2041 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT
     2101 GTACAAGTCC GGACTCAGAT CTCCGAGGGA GCGCAGGGAG CGGGATGCGA AGGAGCGGGA
     2161 CACCATGAAG GAGGACGGCG GCGCGGAGTT CTCGGCTCGC TCCAGGAAGA GGAAGGCAAA
     2221 CGTGACCGTT TTTTTGCAGG ATCCAGATGA AGAAATGGCC AAAATCGACA GGACGGCGAG
     2281 GGACCAGTGT GGGAGCCAGC CTTGGGACAA TAATGCAGTC TGTGCAGACC CCTGCTCCCT
     2341 GATCCCCACA CCTGACAAAG AAGATGATGA CCGGGTTTAC CCAAACTCAA CGTGCAAGCC
     2401 TCGGATTATT GCACCATCCA GAGGCTCCCC GCTGCCTGTA CTGAGCTGGG CAAATAGAGA
     2461 GGAAGTCTGG AAAATCATGT TAAACAAGGA AAAGACATAC TTAAGGGATC AGCACTTTCT
     2521 TGAGCAACAC CCTCTTCTGC AGCCAAAAAT GCGAGCAATT CTTCTGGATT GGTTAATGGA
     2581 GGTGTGTGAA GTCTATAAAC TTCACAGGGA GACCTTTTAC TTGGCACAAG ATTTCTTTGA
     2641 CCGGTATATG GCGACACAAG AAAATGTTGT AAAAACTCTT TTACAGCTTA TTGGGATTTC
     2701 ATCTTTATTT ATTGCAGCCA AACTTGAGGA AATCTATCCT CCAAAGTTGC ACCAGTTTGC
     2761 GTATGTGACA GATGGAGCTT GTTCAGGAGA TGAAATTCTC ACCATGGAAT TAATGATTAT
     2821 GAAGGCCCTT AAGTGGCGTT TAAGTCCCCT GACTATTGTG TCCTGGCTGA ATGTATACAT
     2881 GCAGGTTGCA TATCTAAATG ACTTACATGA AGTGCTACTG CCGCAGTATC CCCAGCAAAT
     2941 CTTTATACAG ATTGCAGAGC TGTTGGATCT CTGTGTCCTG GATGTTGACT GCCTTGAATT
     3001 TCCTTATGGT ATACTTGCTG CTTCGGCCTT GTATCATTTC TCGTCATCTG AATTGATGCA
     3061 AAAGGTTTCA GGGTATCAGT GGTGCGACAT AGAGAACTGT GTCAAGTGGA TGGTTCCATT
     3121 TGCCATGGTT ATAAGGGAGA CGGGGAGCTC AAAACTGAAG CACTTCAGGG GCGTCGCTGA
     3181 TGAAGATGCA CACAACATAC AGACCCACAG AGACAGCTTG GATTTGCTGG ACAAAGCCCG
     3241 AGCAAAGAAA GCCATGTTGT CTGAACAAAA TAGGGCTTCT CCTCTCCCCA GTGGGCTCCT
     3301 CACCCCGCCA CAGAGCGGTA AGAAGCAGAG CAGCGGGCCG GAAATGGCGT GAGTCGACCC
     3361 TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA
     3421 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG
     3481 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
     3541 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC
     3601 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
     3661 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTTACCATG ACCGAGTACA
     3721 AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG GGCCGTACGC ACCCTCGCCG
     3781 CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA TCCGGACCGC CACATCGAGC
     3841 GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT
     3901 GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG
     3961 GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG
     4021 CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC
     4081 TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC
     4141 TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC
     4201 CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGTGCC
     4261 CGAAGGACCG CGCGACCTGG TGCATGACCC GCAAGCCCGG TGCCTGACGC CCGCCCCACG
     4321 ACCCGCAGCG CCCGACCGAA AGGAGCGCAC GACCCCATGC ATCGATAAAA TAAAAGATTT
     4381 TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG
     4441 CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAAATACATA ACTGAGAATA GAGAAGTTCA
     4501 GATCAAGGTC AGGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
     4561 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA
     4621 GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA
     4681 TGCGGTCCAG CCCTCAGCAG TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG
     4741 ACCTGAAATG ACCCTGTGCC TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT
     4801 CGCGCGCTTC TGCTCCCCGA GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA
     4861 GTCCTCCGAT TGACTGAGTC GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG
     4921 CATCCGACTT GTGGTCTCGC TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG
     4981 TCAGCGGGGG TCTTTCATTT CCGACTTGTG GTCTCGCTGC CTTGGGAGGG TCTCCTCTGA
     5041 GTGATTGACT ACCCGTCAGC GGGGGTCTTC ACATGCAGCA TGTATCAAAA TTAATTTGGT
     5101 TTTTTTTCTT AAGTATTTAC ATTAAATGGC CATAGTTGCA TTAATGAATC GGCCAACGCG
     5161 CGGGGAGAGG CGGTTTGCGT ATTGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG
     5221 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
     5281 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
     5341 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     5401 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     5461 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     5521 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     5581 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     5641 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     5701 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     5761 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
     5821 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     5881 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     5941 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
     6001 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA
     6061 GATCCTTTTA AATTAAAAAT GAAGTTTGCG GCCGCAAATC AATCTAAAGT ATATATGAGT
     6121 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
     6181 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
     6241 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
     6301 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
     6361 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
     6421 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
     6481 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
     6541 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
     6601 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
     6661 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
     6721 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA
     6781 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
     6841 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
     6901 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
     6961 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
     7021 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
     7081 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCAC
//