LOCUS       pBABE-mAzG-CCND1a-Puro  6742 bp ds-DNA     circular     28-JUN-2018
DEFINITION  .
KEYWORDS    "creator:Cell Biolabs" "marker:LTR, PuroR, AmpR"
            "organism:Mammalian Cells" "ref:pmid\:2194165"
COMMENT     Retroviral expression vector derived from the Moloney murine
            leukemia virus. Contains a puromycin resistance marker. Strong
            expression is driven by the viral promoter in the 5' LTR.The
            commercially provided sequence was modified to include the splice
            donor mutation. <small>[1] Advanced mammalian gene transfer: high
            titre retroviral vectors with multiple drug selection markers and a
            complementary helper-free packaging cell line. Nucleic Acids Res.
            1990;18:3587-96. Morgenstern JP, Land H. (pmid:2194165)</small>
FEATURES             Location/Qualifiers
     CDS             complement(338..1129)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(1130..1198)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(1199..1303)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             1334..1805
                     /label="LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1867..1867
                     /label="splice donor mutation"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_feature    1868..2067
                     /label="MMLV Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             2260..2676
                     /label="gag (truncated)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    2566..2588
                     /label="pBABE_5` upstream"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2687..2696
                     /label="Kozak"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     gene            2694..3371
                     /label="mAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2694..4295
                     /label="Translation 2694-4295"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVTEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTATSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLLEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPSQAKSGLRSRAQASNSPEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI*"
     misc_feature    3407..3410
                     /label="FRAME"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3411..4295
                     /label="CCND1a"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        4312..4641
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      4492..4627
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4651..5250
                     /label="PuroR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     LTR             5349..5942
                     /label="LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(6314..160)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
       61 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
      121 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
      181 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
      241 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTGC GGCCGCAAAT
      301 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG
      361 CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT
      421 AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG
      481 ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC
      541 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
      601 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
      661 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
      721 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
      781 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
      841 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
      901 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAACACGGG
      961 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
     1021 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
     1081 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
     1141 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
     1201 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
     1261 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
     1321 TGCCACCTGC AGCCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC
     1381 GGCTCAGGGC CAAGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
     1441 AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCAGCCCT
     1501 CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC
     1561 TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT
     1621 CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGG GCGCCAGTCC TCCGATTGAC
     1681 TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG
     1741 TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT
     1801 TCATTTGGGG GCTCGTCCGG GATCGGGAGA CCCCTGCCCA GGGACCACCG ACCCACCACC
     1861 GGGAGGCAAG CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG TCTATGACTG
     1921 ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG CGGACCCGTG
     1981 GTGGAACTGA CGAGTTCTGA ACACCCGGCC GCAACCCTGG GAGACGTCCC AGGGACTTTG
     2041 GGGGCCGTTT TTGTGGCCCG ACCTGAGGAA GGGAGTCGAT GTGGAATCCG ACCCCGTCAG
     2101 GATATGTGGT TCTGGTAGGA GACGAGAACC TAAAACAGTT CCCGCCTCCG TCTGAATTTT
     2161 TGCTTTCGGT TTGGAACCGA AGCCGCGCGT CTTGTCTGCT GCAGCATCGT TCTGTGTTGT
     2221 CTCTGTCTGA CTGTGTTTCT GTATTTGTCT GAAAATTAGG GCCAGACTGT TACCACTCCC
     2281 TTAAGTTTGA CCTTAGATCA CTGGAAAGAT GTCGAGCGGC TCGCTCACAA CCAGTCGGTA
     2341 GATGTCAAGA AGAGACGTTG GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC
     2401 GGATGGCCGC GAGACGGCAC CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC
     2461 TTTTCACCTG GCCCGCATGG ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC
     2521 TTGGCTTTTG ACCCCCCTCC CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT
     2581 CTTCTTCCAT CCGCGCCGTC TCTCCCCCTT GAACCTCCTC TTTCGACCCC GCCTCAATCC
     2641 TCCCTTTATC CAGCCCTCAC TCCTTCTCTA GGCGCCGGCC GGATCCCGCC ACCATGGTGA
     2701 GCGTGATCAA GCCCGAGATG AAGATCAAGC TGTGCATGAG GGGCACCGTG AACGGCCACA
     2761 ACTTCGTGAT CGAGGGCGAG GGCAAGGGCA ACCCCTACGA GGGCACCCAG ATCCTGGACC
     2821 TGAACGTGAC CGAGGGCGCC CCCCTGCCCT TCGCCTACGA CATCCTGACC ACCGTGTTCC
     2881 AGTACGGCAA CAGGGCCTTC ACCAAGTACC CCGCCGACAT CCAGGACTAC TTCAAGCAGA
     2941 CCTTCCCCGA GGGCTACCAC TGGGAGAGGA GCATGACCTA CGAGGACCAG GGCATCTGCA
     3001 CCGCCACCAG CAACATCAGC ATGAGGGGCG ACTGCTTCTT CTACGACATC AGGTTCGACG
     3061 GCACCAACTT CCCCCCCAAC GGCCCCGTGA TGCAGAAGAA GACCCTGAAG TGGGAGCCCA
     3121 GCACCGAGAA GATGTACGTG GAGGACGGCG TGCTGAAGGG CGACGTGAAC ATGAGGCTGC
     3181 TGCTGGAGGG CGGCGGCCAC TACAGGTGCG ACTTCAAGAC CACCTACAAG GCCAAGAAGG
     3241 AGGTGAGGCT GCCCGACGCC CACAAGATCG ACCACAGGAT CGAGATCCTG AAGCACGACA
     3301 AGGACTACAA CAAGGTGAAG CTGTACGAGA ACGCCGTGGC CAGGTACTCC ATGCTGCCCA
     3361 GCCAGGCCAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC GAATTCACCA GAACACCAGC
     3421 TCCTGTGCTG CGAAGTGGAA ACCATCCGCC GCGCGTACCC CGATGCCAAC CTCCTCAACG
     3481 ACCGGGTGCT GCGGGCCATG CTGAAGGCGG AGGAGACCTG CGCGCCCTCG GTGTCCTACT
     3541 TCAAATGTGT GCAGAAGGAG GTCCTGCCGT CCATGCGGAA GATCGTCGCC ACCTGGATGC
     3601 TGGAGGTCTG CGAGGAACAG AAGTGCGAGG AGGAGGTCTT CCCGCTGGCC ATGAACTACC
     3661 TGGACCGCTT CCTGTCGCTG GAGCCCGTGA AAAAGAGCCG CCTGCAGCTG CTGGGGGCCA
     3721 CTTGCATGTT CGTGGCCTCT AAGATGAAGG AGACCATCCC CCTGACGGCC GAGAAGCTGT
     3781 GCATCTACAC CGACAACTCC ATCCGGCCCG AGGAGCTGCT GCAAATGGAG CTGCTCCTGG
     3841 TGAACAAGCT CAAGTGGAAC CTGGCCGCAA TGACCCCGCA CGATTTCATT GAACACTTCC
     3901 TCTCCAAAAT GCCAGAGGCG GAGGAGAACA AACAGATCAT CCGCAAACAC GCGCAGACCT
     3961 TCGTTGCCCT CTGTGCCACA GATGTGAAGT TCATTTCCAA TCCGCCCTCC ATGGTGGCAG
     4021 CGGGGAGCGT GGTGGCCGCA GTGCAAGGCC TGAACCTGAG GAGCCCCAAC AACTTCCTGT
     4081 CCTACTACCG CCTCACACGC TTCCTCTCCA GAGTGATCAA GTGTGACCCG GACTGCCTCC
     4141 GGGCCTGCCA GGAGCAGATC GAAGCCCTGC TGGAGTCAAG CCTGCGCCAG GCCCAGCAGA
     4201 ACATGGACCC CAAGGCCGCC GAGGAGGAGG AAGAGGAGGA GGAGGAGGTG GACCTGGCTT
     4261 GCACACCCAC CGACGTGCGG GACGTGGACA TCTGAGTCGA CCCTGTGGAA TGTGTGTCAG
     4321 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC
     4381 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA
     4441 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC
     4501 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT
     4561 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT
     4621 GGAGGCCTAG GCTTTTGCAA AAAGCTTACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC
     4681 GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC
     4741 CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA
     4801 GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC
     4861 GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG
     4921 ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA
     4981 GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC
     5041 TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG
     5101 GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC
     5161 TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGT GCCCGAAGGA CCGCGCGACC
     5221 TGGTGCATGA CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC
     5281 GAAAGGAGCG CACGACCCCA TGCATCGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA
     5341 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
     5401 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTCAGGAACA
     5461 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
     5521 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
     5581 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
     5641 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
     5701 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
     5761 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGGGCG CCAGTCCTCC GATTGACTGA
     5821 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
     5881 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
     5941 TTTCCGACTT GTGGTCTCGC TGCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC
     6001 AGCGGGGGTC TTCACATGCA GCATGTATCA AAATTAATTT GGTTTTTTTT CTTAAGTATT
     6061 TACATTAAAT GGCCATAGTT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
     6121 CGTATTGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC
     6181 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA
     6241 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG
     6301 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
     6361 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
     6421 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
     6481 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
     6541 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
     6601 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
     6661 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
     6721 TGAAGTGGTG GCCTAACTAC GG
//