LOCUS       pBABE-mAzG-CCND3-Puro   6733 bp ds-DNA     circular     16-JUN-2016
DEFINITION  .
KEYWORDS    "creator:Cell Biolabs" "marker:LTR, PuroR, AmpR"
            "organism:Mammalian Cells" "ref:pmid\:2194165"
COMMENT     Retroviral expression vector derived from the Moloney murine
            leukemia virus. Contains a puromycin resistance marker. Strong
            expression is driven by the viral promoter in the 5' LTR.The
            commercially provided sequence was modified to include the splice
            donor mutation. <small>[1] Advanced mammalian gene transfer: high
            titre retroviral vectors with multiple drug selection markers and a
            complementary helper-free packaging cell line. Nucleic Acids Res.
            1990;18:3587-96. Morgenstern JP, Land H. (pmid:2194165)</small>
FEATURES             Location/Qualifiers
     LTR             8..479
                     /label="LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    541..541
                     /label="splice donor mutation"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_feature    542..741
                     /label="MMLV Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             934..1350
                     /label="gag (truncated)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    1361..1370
                     /label="Kozak"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     gene            1368..2045
                     /label="mAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1368..2084
                     /label="Translation 1368-2084"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVTEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTATSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLLEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPSQAKSGLRSRAQASNSP"
     misc_feature    2081..2084
                     /label="FRAME"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2085..2960
                     /label="CCND3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             2085..2960
                     /label="Translation 2085-2960"
                     /translation="ELLCCEGTRHAPRAGPDPRLLGDQRVLQSLLRLEERYVPRASYFQCVQREIKPHMRKMLAYWMLEVCEEQRCEEEVFPLAMNYLDRYLSCVPTRKAQLQLLGAVCMLLASKLRETTPLTIEKLCIYTDHAVSPRQLRDWEVLVLGKLKWDLAAVIAHDFLAFILHRLSLPRDRQALVKKHAQTFLALCATDYTFAMYPPSMIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL*"
     promoter        2977..3306
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      3157..3292
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3316..3915
                     /label="PuroR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     LTR             4014..4607
                     /label="LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(4979..5567)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(5745..6536)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(6537..6605)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(6606..6710)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 CTGCAGCCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
       61 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT
      121 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG
      181 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC
      241 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA
      301 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC
      361 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC
      421 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATTT
      481 GGGGGCTCGT CCGGGATCGG GAGACCCCTG CCCAGGGACC ACCGACCCAC CACCGGGAGG
      541 CAAGCTGGCC AGCAACTTAT CTGTGTCTGT CCGATTGTCT AGTGTCTATG ACTGATTTTA
      601 TGCGCCTGCG TCGGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA
      661 CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC
      721 GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG
      781 TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT
      841 CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT
      901 CTGACTGTGT TTCTGTATTT GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT
      961 TTGACCTTAG ATCACTGGAA AGATGTCGAG CGGCTCGCTC ACAACCAGTC GGTAGATGTC
     1021 AAGAAGAGAC GTTGGGTTAC CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG
     1081 CCGCGAGACG GCACCTTTAA CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA
     1141 CCTGGCCCGC ATGGACACCC AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT
     1201 TTTGACCCCC CTCCCTGGGT CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCTT
     1261 CCATCCGCGC CGTCTCTCCC CCTTGAACCT CCTCTTTCGA CCCCGCCTCA ATCCTCCCTT
     1321 TATCCAGCCC TCACTCCTTC TCTAGGCGCC GGCCGGATCC CGCCACCATG GTGAGCGTGA
     1381 TCAAGCCCGA GATGAAGATC AAGCTGTGCA TGAGGGGCAC CGTGAACGGC CACAACTTCG
     1441 TGATCGAGGG CGAGGGCAAG GGCAACCCCT ACGAGGGCAC CCAGATCCTG GACCTGAACG
     1501 TGACCGAGGG CGCCCCCCTG CCCTTCGCCT ACGACATCCT GACCACCGTG TTCCAGTACG
     1561 GCAACAGGGC CTTCACCAAG TACCCCGCCG ACATCCAGGA CTACTTCAAG CAGACCTTCC
     1621 CCGAGGGCTA CCACTGGGAG AGGAGCATGA CCTACGAGGA CCAGGGCATC TGCACCGCCA
     1681 CCAGCAACAT CAGCATGAGG GGCGACTGCT TCTTCTACGA CATCAGGTTC GACGGCACCA
     1741 ACTTCCCCCC CAACGGCCCC GTGATGCAGA AGAAGACCCT GAAGTGGGAG CCCAGCACCG
     1801 AGAAGATGTA CGTGGAGGAC GGCGTGCTGA AGGGCGACGT GAACATGAGG CTGCTGCTGG
     1861 AGGGCGGCGG CCACTACAGG TGCGACTTCA AGACCACCTA CAAGGCCAAG AAGGAGGTGA
     1921 GGCTGCCCGA CGCCCACAAG ATCGACCACA GGATCGAGAT CCTGAAGCAC GACAAGGACT
     1981 ACAACAAGGT GAAGCTGTAC GAGAACGCCG TGGCCAGGTA CTCCATGCTG CCCAGCCAGG
     2041 CCAAGTCCGG ACTCAGATCT CGAGCTCAAG CTTCGAATTC ACCAGAGCTG CTGTGTTGCG
     2101 AAGGCACCCG GCACGCGCCC CGGGCCGGGC CGGACCCGCG GCTGCTGGGG GACCAGCGTG
     2161 TCCTGCAGAG CCTGCTCCGC CTGGAGGAGC GCTACGTACC CCGCGCCTCC TACTTCCAGT
     2221 GCGTGCAGCG GGAGATCAAG CCGCACATGC GGAAGATGCT GGCTTACTGG ATGCTGGAGG
     2281 TATGTGAGGA GCAGCGCTGT GAGGAGGAAG TCTTCCCCCT GGCCATGAAC TACCTGGATC
     2341 GCTACCTGTC TTGCGTCCCC ACCCGAAAGG CGCAGTTGCA GCTCCTGGGT GCGGTCTGCA
     2401 TGCTGCTGGC CTCCAAGCTG CGCGAGACCA CGCCCCTGAC CATCGAAAAA CTGTGCATCT
     2461 ACACCGACCA CGCTGTCTCT CCCCGCCAGT TGCGGGACTG GGAGGTGCTG GTCCTAGGGA
     2521 AGCTCAAGTG GGACCTGGCT GCTGTGATTG CACATGATTT CCTGGCCTTC ATTCTGCACC
     2581 GGCTCTCTCT GCCCCGTGAC CGACAGGCCT TGGTCAAAAA GCATGCCCAG ACCTTTTTGG
     2641 CCCTCTGTGC TACAGATTAT ACCTTTGCCA TGTACCCGCC ATCCATGATC GCCACGGGCA
     2701 GCATTGGGGC TGCAGTGCAA GGCCTGGGTG CCTGCTCCAT GTCCGGGGAT GAGCTCACAG
     2761 AGCTGCTGGC AGGGATCACT GGCACTGAAG TGGACTGCCT GCGGGCCTGT CAGGAGCAGA
     2821 TCGAAGCTGC ACTCAGGGAG AGCCTCAGGG AAGCCTCTCA GACCAGCTCC AGCCCAGCGC
     2881 CCAAAGCCCC CCGGGGCTCC AGCAGCCAAG GGCCCAGCCA GACCAGCACT CCTACAGATG
     2941 TCACAGCCAT ACACCTGTAG GTCGACCCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG
     3001 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC
     3061 AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT
     3121 TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT
     3181 TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC
     3241 GCCTCGGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
     3301 TGCAAAAAGC TTACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC
     3361 GTCCCCAGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC
     3421 ACCGTCGATC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG
     3481 CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC
     3541 TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CCCGCGCATG
     3601 GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG
     3661 CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG
     3721 GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCCGGG
     3781 GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC
     3841 TTCACCGTCA CCGCCGACGT CGAGTGCCCG AAGGACCGCG CGACCTGGTG CATGACCCGC
     3901 AAGCCCGGTG CCTGACGCCC GCCCCACGAC CCGCAGCGCC CGACCGAAAG GAGCGCACGA
     3961 CCCCATGCAT CGATAAAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG GGGAATGAAA
     4021 GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA GGCATGGAAA
     4081 AATACATAAC TGAGAATAGA GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA
     4141 TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA
     4201 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
     4261 TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT TCTAGAGAAC
     4321 CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA
     4381 ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG
     4441 AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC
     4501 GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA
     4561 GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCATTTCC GACTTGTGGT
     4621 CTCGCTGCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTCAC
     4681 ATGCAGCATG TATCAAAATT AATTTGGTTT TTTTTCTTAA GTATTTACAT TAAATGGCCA
     4741 TAGTTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGCGCTCTT
     4801 CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG
     4861 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA
     4921 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT
     4981 TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC
     5041 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
     5101 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
     5161 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
     5221 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT
     5281 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA
     5341 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
     5401 ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
     5461 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
     5521 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA
     5581 TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA
     5641 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTGCGGC
     5701 CGCAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     5761 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     5821 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     5881 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     5941 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     6001 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     6061 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     6121 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     6181 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     6241 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     6301 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     6361 ACACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     6421 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     6481 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     6541 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     6601 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     6661 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC
     6721 CGAAAAGTGC CAC
//