LOCUS       NES-mAzG-CCND1a_pBABE.p 6808 bp ds-DNA     circular     12-APR-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     LTR             8..479
                     /label="LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    541..541
                     /label="splice donor mutation"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_feature    542..741
                     /label="MMLV Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             934..1350
                     /label="gag (truncated)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    1361..1367
                     /label="kozak"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1368..1418
                     /label="Super-PKI NES"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1368..1418
                     /label="Translation 1368-1418"
                     /translation="MGADIDELALKFAGLDL"
     CDS             1419..1433
                     /label="Translation 1419-1433"
                     /translation="GPAKS"
     misc_feature    1419..1436
                     /label="linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1434..1436
                     /label="Translation 1434-1436"
                     /translation="P"
     gene            1437..2111
                     /label="mAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1437..3035
                     /label="Translation 1437-3035"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVTEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTATSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLLEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPSQAKSGLRSRAQASNSPEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI*"
     misc_feature    2147..2150
                     /label="FRAME"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2151..3035
                     /label="CCND1a"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        3052..3381
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      3232..3367
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3391..3990
                     /label="PuroR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     LTR             4089..4682
                     /label="LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(5054..5642)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(5820..6611)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(6612..6680)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(6681..6785)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 CTGCAGCCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
       61 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT
      121 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG
      181 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC
      241 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA
      301 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC
      361 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC
      421 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATTT
      481 GGGGGCTCGT CCGGGATCGG GAGACCCCTG CCCAGGGACC ACCGACCCAC CACCGGGAGG
      541 CAAGCTGGCC AGCAACTTAT CTGTGTCTGT CCGATTGTCT AGTGTCTATG ACTGATTTTA
      601 TGCGCCTGCG TCGGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA
      661 CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC
      721 GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG
      781 TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT
      841 CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT
      901 CTGACTGTGT TTCTGTATTT GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT
      961 TTGACCTTAG ATCACTGGAA AGATGTCGAG CGGCTCGCTC ACAACCAGTC GGTAGATGTC
     1021 AAGAAGAGAC GTTGGGTTAC CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG
     1081 CCGCGAGACG GCACCTTTAA CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA
     1141 CCTGGCCCGC ATGGACACCC AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT
     1201 TTTGACCCCC CTCCCTGGGT CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCTT
     1261 CCATCCGCGC CGTCTCTCCC CCTTGAACCT CCTCTTTCGA CCCCGCCTCA ATCCTCCCTT
     1321 TATCCAGCCC TCACTCCTTC TCTAGGCGCC GGCCGGATCC CGCCACCATG GGAGCAGACA
     1381 TTGATGAACT TGCGCTCAAA TTCGCTGGAC TTGATTTGGG ACCGGCTAAG TCACCAGTGA
     1441 GCGTGATCAA GCCCGAGATG AAGATCAAGC TGTGCATGAG GGGCACCGTG AACGGCCACA
     1501 ACTTCGTGAT CGAGGGCGAG GGCAAGGGCA ACCCCTACGA GGGCACCCAG ATCCTGGACC
     1561 TGAACGTGAC CGAGGGCGCC CCCCTGCCCT TCGCCTACGA CATCCTGACC ACCGTGTTCC
     1621 AGTACGGCAA CAGGGCCTTC ACCAAGTACC CCGCCGACAT CCAGGACTAC TTCAAGCAGA
     1681 CCTTCCCCGA GGGCTACCAC TGGGAGAGGA GCATGACCTA CGAGGACCAG GGCATCTGCA
     1741 CCGCCACCAG CAACATCAGC ATGAGGGGCG ACTGCTTCTT CTACGACATC AGGTTCGACG
     1801 GCACCAACTT CCCCCCCAAC GGCCCCGTGA TGCAGAAGAA GACCCTGAAG TGGGAGCCCA
     1861 GCACCGAGAA GATGTACGTG GAGGACGGCG TGCTGAAGGG CGACGTGAAC ATGAGGCTGC
     1921 TGCTGGAGGG CGGCGGCCAC TACAGGTGCG ACTTCAAGAC CACCTACAAG GCCAAGAAGG
     1981 AGGTGAGGCT GCCCGACGCC CACAAGATCG ACCACAGGAT CGAGATCCTG AAGCACGACA
     2041 AGGACTACAA CAAGGTGAAG CTGTACGAGA ACGCCGTGGC CAGGTACTCC ATGCTGCCCA
     2101 GCCAGGCCAA GTCCGGACTC AGATCTCGAG CTCAAGCTTC GAATTCACCA GAACACCAGC
     2161 TCCTGTGCTG CGAAGTGGAA ACCATCCGCC GCGCGTACCC CGATGCCAAC CTCCTCAACG
     2221 ACCGGGTGCT GCGGGCCATG CTGAAGGCGG AGGAGACCTG CGCGCCCTCG GTGTCCTACT
     2281 TCAAATGTGT GCAGAAGGAG GTCCTGCCGT CCATGCGGAA GATCGTCGCC ACCTGGATGC
     2341 TGGAGGTCTG CGAGGAACAG AAGTGCGAGG AGGAGGTCTT CCCGCTGGCC ATGAACTACC
     2401 TGGACCGCTT CCTGTCGCTG GAGCCCGTGA AAAAGAGCCG CCTGCAGCTG CTGGGGGCCA
     2461 CTTGCATGTT CGTGGCCTCT AAGATGAAGG AGACCATCCC CCTGACGGCC GAGAAGCTGT
     2521 GCATCTACAC CGACAACTCC ATCCGGCCCG AGGAGCTGCT GCAAATGGAG CTGCTCCTGG
     2581 TGAACAAGCT CAAGTGGAAC CTGGCCGCAA TGACCCCGCA CGATTTCATT GAACACTTCC
     2641 TCTCCAAAAT GCCAGAGGCG GAGGAGAACA AACAGATCAT CCGCAAACAC GCGCAGACCT
     2701 TCGTTGCCCT CTGTGCCACA GATGTGAAGT TCATTTCCAA TCCGCCCTCC ATGGTGGCAG
     2761 CGGGGAGCGT GGTGGCCGCA GTGCAAGGCC TGAACCTGAG GAGCCCCAAC AACTTCCTGT
     2821 CCTACTACCG CCTCACACGC TTCCTCTCCA GAGTGATCAA GTGTGACCCG GACTGCCTCC
     2881 GGGCCTGCCA GGAGCAGATC GAAGCCCTGC TGGAGTCAAG CCTGCGCCAG GCCCAGCAGA
     2941 ACATGGACCC CAAGGCCGCC GAGGAGGAGG AAGAGGAGGA GGAGGAGGTG GACCTGGCTT
     3001 GCACACCCAC CGACGTGCGG GACGTGGACA TCTGAGTCGA CCCTGTGGAA TGTGTGTCAG
     3061 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC
     3121 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA
     3181 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC
     3241 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT
     3301 GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT
     3361 GGAGGCCTAG GCTTTTGCAA AAAGCTTACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC
     3421 GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC
     3481 CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA
     3541 GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC
     3601 GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG
     3661 ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA
     3721 GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC
     3781 TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG
     3841 GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC
     3901 TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGT GCCCGAAGGA CCGCGCGACC
     3961 TGGTGCATGA CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC
     4021 GAAAGGAGCG CACGACCCCA TGCATCGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA
     4081 AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
     4141 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG GTCAGGAACA
     4201 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
     4261 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
     4321 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
     4381 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
     4441 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
     4501 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGGGCG CCAGTCCTCC GATTGACTGA
     4561 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
     4621 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
     4681 TTTCCGACTT GTGGTCTCGC TGCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC
     4741 AGCGGGGGTC TTCACATGCA GCATGTATCA AAATTAATTT GGTTTTTTTT CTTAAGTATT
     4801 TACATTAAAT GGCCATAGTT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG
     4861 CGTATTGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC
     4921 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA
     4981 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG
     5041 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
     5101 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
     5161 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
     5221 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
     5281 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
     5341 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
     5401 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
     5461 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC
     5521 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
     5581 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
     5641 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT
     5701 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA
     5761 AATGAAGTTT GCGGCCGCAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     5821 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     5881 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     5941 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     6001 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     6061 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     6121 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     6181 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     6241 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     6301 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     6361 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     6421 TCTTGCCCGG CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     6481 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     6541 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     6601 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     6661 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     6721 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     6781 CCGCGCACAT TTCCCCGAAA AGTGCCAC
//