LOCUS       NLS-mAzG-CCND1a(HP)_pBA 6790 bp ds-DNA     circular     12-APR-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     LTR             8..479
                     /label="LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    541..541
                     /label="splice donor mutation"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
     misc_feature    542..741
                     /label="MMLV Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             934..1350
                     /label="gag (truncated)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    1361..1367
                     /label="kozak"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1368..1400
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             1368..1400
                     /label="Translation 1368-1400"
                     /translation="MGASPKKKRKV"
     CDS             1401..1415
                     /label="Translation 1401-1415"
                     /translation="GPAKS"
     misc_feature    1401..1418
                     /label="linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1416..1418
                     /label="Translation 1416-1418"
                     /translation="P"
     gene            1419..2093
                     /label="mAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1419..3017
                     /label="Translation 1419-3017"
                     /translation="VSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNVTEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTATSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLLLEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLPSQAKSGLRSRAQASNSPEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSARKIAATAMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI*"
     misc_feature    2129..2132
                     /label="FRAME"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2133..3017
                     /label="CCND1a"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        3034..3363
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      3214..3349
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3373..3972
                     /label="PuroR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     LTR             4071..4664
                     /label="LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(5036..5624)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(5802..6593)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(6594..6662)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(6663..6767)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 CTGCAGCCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
       61 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT
      121 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG
      181 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC
      241 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA
      301 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC
      361 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC
      421 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATTT
      481 GGGGGCTCGT CCGGGATCGG GAGACCCCTG CCCAGGGACC ACCGACCCAC CACCGGGAGG
      541 CAAGCTGGCC AGCAACTTAT CTGTGTCTGT CCGATTGTCT AGTGTCTATG ACTGATTTTA
      601 TGCGCCTGCG TCGGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA
      661 CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC
      721 GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG
      781 TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT
      841 CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT
      901 CTGACTGTGT TTCTGTATTT GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT
      961 TTGACCTTAG ATCACTGGAA AGATGTCGAG CGGCTCGCTC ACAACCAGTC GGTAGATGTC
     1021 AAGAAGAGAC GTTGGGTTAC CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG
     1081 CCGCGAGACG GCACCTTTAA CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA
     1141 CCTGGCCCGC ATGGACACCC AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT
     1201 TTTGACCCCC CTCCCTGGGT CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCTT
     1261 CCATCCGCGC CGTCTCTCCC CCTTGAACCT CCTCTTTCGA CCCCGCCTCA ATCCTCCCTT
     1321 TATCCAGCCC TCACTCCTTC TCTAGGCGCC GGCCGGATCC CGCCACCATG GGAGCATCAC
     1381 CAAAAAAGAA GAGAAAGGTA GGACCGGCTA AGTCACCAGT GAGCGTGATC AAGCCCGAGA
     1441 TGAAGATCAA GCTGTGCATG AGGGGCACCG TGAACGGCCA CAACTTCGTG ATCGAGGGCG
     1501 AGGGCAAGGG CAACCCCTAC GAGGGCACCC AGATCCTGGA CCTGAACGTG ACCGAGGGCG
     1561 CCCCCCTGCC CTTCGCCTAC GACATCCTGA CCACCGTGTT CCAGTACGGC AACAGGGCCT
     1621 TCACCAAGTA CCCCGCCGAC ATCCAGGACT ACTTCAAGCA GACCTTCCCC GAGGGCTACC
     1681 ACTGGGAGAG GAGCATGACC TACGAGGACC AGGGCATCTG CACCGCCACC AGCAACATCA
     1741 GCATGAGGGG CGACTGCTTC TTCTACGACA TCAGGTTCGA CGGCACCAAC TTCCCCCCCA
     1801 ACGGCCCCGT GATGCAGAAG AAGACCCTGA AGTGGGAGCC CAGCACCGAG AAGATGTACG
     1861 TGGAGGACGG CGTGCTGAAG GGCGACGTGA ACATGAGGCT GCTGCTGGAG GGCGGCGGCC
     1921 ACTACAGGTG CGACTTCAAG ACCACCTACA AGGCCAAGAA GGAGGTGAGG CTGCCCGACG
     1981 CCCACAAGAT CGACCACAGG ATCGAGATCC TGAAGCACGA CAAGGACTAC AACAAGGTGA
     2041 AGCTGTACGA GAACGCCGTG GCCAGGTACT CCATGCTGCC CAGCCAGGCC AAGTCCGGAC
     2101 TCAGATCTCG AGCTCAAGCT TCGAATTCAC CAGAACACCA GCTCCTGTGC TGCGAAGTGG
     2161 AAACCATCCG CCGCGCGTAC CCCGATGCCA ACCTCCTCAA CGACCGGGTG CTGCGGGCCA
     2221 TGCTGAAGGC GGAGGAGACC TGCGCGCCCT CGGTGTCCTA CTTCAAATGT GTGCAGAAGG
     2281 AGGTCCTGCC GTCCGCGCGG AAGATCGCCG CCACCGCGAT GCTGGAGGTC TGCGAGGAAC
     2341 AGAAGTGCGA GGAGGAGGTC TTCCCGCTGG CCATGAACTA CCTGGACCGC TTCCTGTCGC
     2401 TGGAGCCCGT GAAAAAGAGC CGCCTGCAGC TGCTGGGGGC CACTTGCATG TTCGTGGCCT
     2461 CTAAGATGAA GGAGACCATC CCCCTGACGG CCGAGAAGCT GTGCATCTAC ACCGACAACT
     2521 CCATCCGGCC CGAGGAGCTG CTGCAAATGG AGCTGCTCCT GGTGAACAAG CTCAAGTGGA
     2581 ACCTGGCCGC AATGACCCCG CACGATTTCA TTGAACACTT CCTCTCCAAA ATGCCAGAGG
     2641 CGGAGGAGAA CAAACAGATC ATCCGCAAAC ACGCGCAGAC CTTCGTTGCC CTCTGTGCCA
     2701 CAGATGTGAA GTTCATTTCC AATCCGCCCT CCATGGTGGC AGCGGGGAGC GTGGTGGCCG
     2761 CAGTGCAAGG CCTGAACCTG AGGAGCCCCA ACAACTTCCT GTCCTACTAC CGCCTCACAC
     2821 GCTTCCTCTC CAGAGTGATC AAGTGTGACC CGGACTGCCT CCGGGCCTGC CAGGAGCAGA
     2881 TCGAAGCCCT GCTGGAGTCA AGCCTGCGCC AGGCCCAGCA GAACATGGAC CCCAAGGCCG
     2941 CCGAGGAGGA GGAAGAGGAG GAGGAGGAGG TGGACCTGGC TTGCACACCC ACCGACGTGC
     3001 GGGACGTGGA CATCTGAGTC GACCCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC
     3061 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG
     3121 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG
     3181 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC
     3241 GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC
     3301 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
     3361 AAAAAGCTTA CCATGACCGA GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC
     3421 CCCAGGGCCG TACGCACCCT CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC
     3481 GTCGATCCGG ACCGCCACAT CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC
     3541 GTCGGGCTCG ACATCGGCAA GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG
     3601 ACCACGCCGG AGAGCGTCGA AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC
     3661 GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC
     3721 CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC
     3781 AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG
     3841 CCCGCCTTCC TGGAGACCTC CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC
     3901 ACCGTCACCG CCGACGTCGA GTGCCCGAAG GACCGCGCGA CCTGGTGCAT GACCCGCAAG
     3961 CCCGGTGCCT GACGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC
     4021 CATGCATCGA TAAAATAAAA GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC
     4081 CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT
     4141 ACATAACTGA GAATAGAGAA GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT
     4201 GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT
     4261 GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
     4321 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT
     4381 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC
     4441 AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC
     4501 CCACAACCCC TCACTCGGGG CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG
     4561 TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG
     4621 TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATTTCCGAC TTGTGGTCTC
     4681 GCTGCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTCACATG
     4741 CAGCATGTAT CAAAATTAAT TTGGTTTTTT TTCTTAAGTA TTTACATTAA ATGGCCATAG
     4801 TTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG CGCTCTTCCG
     4861 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
     4921 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
     4981 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     5041 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     5101 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     5161 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     5221 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     5281 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     5341 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     5401 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     5461 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     5521 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     5581 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
     5641 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     5701 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTGCGGCCGC
     5761 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     5821 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     5881 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     5941 CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     6001 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     6061 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     6121 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     6181 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     6241 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     6301 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     6361 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA
     6421 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     6481 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     6541 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     6601 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     6661 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     6721 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA
     6781 AAAGTGCCAC
//