LOCUS       3xHA-TurboID-13x-CCND1_ 7337 bp ds-DNA     circular     27-NOV-2018
DEFINITION  .
KEYWORDS    "marker:AmpR, PuroR, LTR, LTR" "ref:pmid\:22412018"
COMMENT     To fuse your protein of interest to the C-terminus of BirA(R118G)
            and use in proximity-dependent biotin identification (BioID); myc
            tag. <small>[1] A promiscuous biotin ligase fusion protein
            identifies proximal and interacting proteins in mammalian cells. J
            Cell Biol. 2012 Mar 12. Roux KJ, Kim DI, Raida M, Burke B
            (pmid:22412018)</small>
FEATURES             Location/Qualifiers
     LTR             8..477
                     /label="LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    542..741
                     /label="MMLV Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             942..1358
                     /label="gag (truncated)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    1393..1400
                     /label="NotI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1405..2457
                     /label="Translation 1405-2457"
                     /translation="MYPYDVPDYAGYPYDVPDYAGYPYDVPDYAASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             1408..1434
                     /label="HA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             1438..1464
                     /label="HA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1468..1494
                     /label="HA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1495..1500
                     /label="NheI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1501..2457
                     /label="BirA (humanized)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1690..1692
                     /label="Q65P"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1756..1758
                     /label="I87V"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1840..1842
                     /label="G115G"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1849..1851
                     /label="R118S"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1915..1917
                     /label="E141K"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_difference 1918..1920
                     /label="Q142R"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1942..1944
                     /label="S150G"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1945..1947
                     /label="L151P"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1972..1974
                     /label="V160A"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2068..2070
                     /label="T192A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_difference 2074..2076
                     /label="K194I"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    2119..2121
                     /label="M209V"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2215..2217
                     /label="M241T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2281..2283
                     /label="S263P"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_structure  2365..2367
                     /label="G291G"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2407..2409
                     /label="I305V"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             2458..2463
                     /label="Translation 2458-2463"
                     /translation="LE"
     misc_feature    2464..2667
                     /label="13xLinker"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             2464..2667
                     /label="Translation 2464-2667"
                     /translation="GSGGGGSGGGGSGGGGSGGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGGGS"
     CDS             2668..2670
                     /label="Translation 2668-2670"
                     /translation="A"
     misc_feature    2671..2671
                     /label="FRAME"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2671..2679
                     /label="Translation 2671-2679"
                     /translation="GLN"
     misc_feature    2680..3564
                     /label="CCND1a"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2680..3564
                     /label="Translation 2680-3564"
                     /translation="EHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI*"
     misc_feature    2689..2703
                     /label="Rb binding"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2842..2856
                     /label="Hydrophobic p."
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2935..2943
                     /label="RxL put.?"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2971..2982
                     /label="Hydrophobic p."
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        3581..3910
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      3761..3896
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3920..4519
                     /label="PuroR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     LTR             4618..5211
                     /label="LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      complement(5583..6171)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(6349..7140)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(7141..7209)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(7210..7314)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
      901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
      961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
     1381 acgtaggaat tcgcggccgc caccatgtac ccgtatgatg ttccggatta cgctggctat
     1441 ccctacgacg tgcccgacta tgccgggtac ccctatgacg tcccagacta cgcagctagc
     1501 aaagacaata ctgtgcctct gaagctgatc gctctcctgg ctaatggcga gttccatagt
     1561 ggcgaacagc tgggagaaac cctgggcatg tccagggccg ctatcaacaa gcacattcag
     1621 actctgcgcg actggggcgt ggacgtgttc accgtgcccg gaaagggcta ctctctgccc
     1681 gagcctatcc cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg
     1741 gcagtcctgc ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg
     1801 aagagtgggg atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg
     1861 aaatggttct ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg
     1921 ggaccagcag caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga
     1981 aagctgggag cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga
     2041 aagctggcag gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc
     2101 attggcgccg ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc
     2161 tggatcacac tgcaggaagc agggattaac ctggacagga atactctggc cgctacgctg
     2221 atccgagagc tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg
     2281 ccacggtggg agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac
     2341 aaagagattt tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag
     2401 gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaagctc
     2461 gagggatccg gtggaggcgg gtctggaggc gggggtagtg gcgggggtgg aagcgggggt
     2521 ggaggcgggt cgggtggcgg aggtagcgga ggcggtggaa gtggaggcgg tggaagtggt
     2581 ggaggaggtt ctggtggcgg aggtagcggt ggcggaggta gcggaggcgg tggaagtggt
     2641 ggcggaggta gcggaggcgg tggatcggcg GgtttaaacG AACACCAGCT CCTGTGCTGC
     2701 GAAGTGGAAA CCATCCGCCG CGCGTACCCC GATGCCAACC TCCTCAACGA CCGGGTGCTG
     2761 CGGGCCATGC TGAAGGCGGA GGAGACCTGC GCGCCCTCGG TGTCCTACTT CAAATGTGTG
     2821 CAGAAGGAGG TCCTGCCGTC CATGCGGAAG ATCGTCGCCA CCTGGATGCT GGAGGTCTGC
     2881 GAGGAACAGA AGTGCGAGGA GGAGGTCTTC CCGCTGGCCA TGAACTACCT GGACCGCTTC
     2941 CTGTCGCTGG AGCCCGTGAA AAAGAGCCGC CTGCAGCTGC TGGGGGCCAC TTGCATGTTC
     3001 GTGGCCTCTA AGATGAAGGA GACCATCCCC CTGACGGCCG AGAAGCTGTG CATCTACACC
     3061 GACAACTCCA TCCGGCCCGA GGAGCTGCTG CAAATGGAGC TGCTCCTGGT GAACAAGCTC
     3121 AAGTGGAACC TGGCCGCAAT GACCCCGCAC GATTTCATTG AACACTTCCT CTCCAAAATG
     3181 CCAGAGGCGG AGGAGAACAA ACAGATCATC CGCAAACACG CGCAGACCTT CGTTGCCCTC
     3241 TGTGCCACAG ATGTGAAGTT CATTTCCAAT CCGCCCTCCA TGGTGGCAGC GGGGAGCGTG
     3301 GTGGCCGCAG TGCAAGGCCT GAACCTGAGG AGCCCCAACA ACTTCCTGTC CTACTACCGC
     3361 CTCACACGCT TCCTCTCCAG AGTGATCAAG TGTGACCCAG ACTGCCTCCG GGCCTGCCAG
     3421 GAGCAGATCG AAGCCCTGCT GGAGTCAAGC CTGCGCCAGG CCCAGCAGAA CATGGACCCC
     3481 AAGGCCGCCG AGGAGGAGGA AGAGGAGGAG GAGGAGGTGG ACCTGGCTTG CACACCCACC
     3541 GACGTGCGGG ACGTGGACAT CTGAgtcgac cctgtggaat gtgtgtcagt tagggtgtgg
     3601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3661 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3721 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3781 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3841 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3901 cttttgcaaa aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3961 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     4021 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     4081 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     4141 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     4201 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4261 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4321 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4381 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4441 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4501 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     4561 acgaccccat gcatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     4621 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     4681 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc
     4741 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     4801 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4861 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
     4921 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     4981 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     5041 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     5101 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     5161 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttccgacttg
     5221 tggtctcgct gccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     5281 tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt acattaaatg
     5341 gccatagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattggcgc
     5401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5761 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     5941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6001 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     6061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttg
     6301 cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6361 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6421 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6481 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6541 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6601 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6661 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6721 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6781 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6841 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6901 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6961 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7021 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7081 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7141 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7201 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7261 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7321 tccccgaaaa gtgccac
//