LOCUS       3xHA-TurboID-CCND1a-pBA 7183 bp ds-DNA     circular     20-NOV-2018
DEFINITION  .
KEYWORDS    "marker:AmpR, PuroR, LTR, LTR" "ref:pmid\:22412018"
COMMENT     To fuse your protein of interest to the C-terminus of BirA(R118G)
            and use in proximity-dependent biotin identification (BioID); myc
            tag. <small>[1] A promiscuous biotin ligase fusion protein
            identifies proximal and interacting proteins in mammalian cells. J
            Cell Biol. 2012 Mar 12. Roux KJ, Kim DI, Raida M, Burke B
            (pmid:22412018)</small>
FEATURES             Location/Qualifiers
     rep_origin      complement(487..1075)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(1253..2044)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(2045..2113)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        complement(2114..2218)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     LTR             2249..2718
                     /label="LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2783..2982
                     /label="MMLV Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             3183..3599
                     /label="gag (truncated)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    3634..3641
                     /label="NotI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3646..4698
                     /label="Translation 3646-4698"
                     /translation="MYPYDVPDYAGYPYDVPDYAGYPYDVPDYAASKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             3649..3675
                     /label="HA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             3679..3705
                     /label="HA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             3709..3735
                     /label="HA"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3736..3741
                     /label="NheI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3742..4698
                     /label="BirA (humanized)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3931..3933
                     /label="Q65P"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3997..3999
                     /label="I87V"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    4081..4083
                     /label="G115G"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4090..4092
                     /label="R118S"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4156..4158
                     /label="E141K"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_difference 4159..4161
                     /label="Q142R"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4183..4185
                     /label="S150G"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    4186..4188
                     /label="L151P"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4213..4215
                     /label="V160A"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4309..4311
                     /label="T192A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_difference 4315..4317
                     /label="K194I"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4360..4362
                     /label="M209V"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4456..4458
                     /label="M241T"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4522..4524
                     /label="S263P"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_structure  4606..4608
                     /label="G291G"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    4648..4650
                     /label="I305V"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             4699..4704
                     /label="Translation 4699-4704"
                     /translation="LE"
     misc_feature    4705..4749
                     /label="Linker"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             4705..4749
                     /label="Translation 4705-4749"
                     /translation="GGGGSGGGGSGGGGS"
     CDS             4750..4752
                     /label="Translation 4750-4752"
                     /translation="P"
     misc_feature    4750..5637
                     /label="CCND1a"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             4753..5637
                     /label="Translation 4753-5637"
                     /translation="EHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI*"
     misc_feature    4762..4776
                     /label="Rb binding"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4915..4929
                     /label="Hydrophobic p."
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5008..5016
                     /label="RxL put.?"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    5044..5055
                     /label="Hydrophobic p."
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5668..5997
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      5848..5983
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             6007..6606
                     /label="PuroR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     LTR             6705..115
                     /label="LTR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
ORIGIN
        1 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
       61 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttccga
      121 cttgtggtct cgctgccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
      181 gtcttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt atttacatta
      241 aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
      301 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      361 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      421 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      481 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      541 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      601 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      661 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      721 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      781 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      841 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      901 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
      961 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1021 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1081 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1141 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1201 tttgcggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1861 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2221 catttccccg aaaagtgcca cctgcagcct gaatatgggc caaacaggat atctgtggta
     2281 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     2341 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     2401 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     2461 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     2521 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     2581 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     2641 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     2701 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     2761 caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc
     2821 tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta
     2881 tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac cctgggagac
     2941 gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag tcgatgtgga
     3001 atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
     3061 ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc
     3121 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt
     3181 agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc
     3241 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg
     3301 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca
     3361 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
     3421 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
     3481 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
     3541 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg
     3601 gccggatccc agtgtggtgg tacgtaggaa ttcgcggccg ccaccatgta cccgtatgat
     3661 gttccggatt acgctggcta tccctacgac gtgcccgact atgccgggta cccctatgac
     3721 gtcccagact acgcagctag caaagacaat actgtgcctc tgaagctgat cgctctcctg
     3781 gctaatggcg agttccatag tggcgaacag ctgggagaaa ccctgggcat gtccagggcc
     3841 gctatcaaca agcacattca gactctgcgc gactggggcg tggacgtgtt caccgtgccc
     3901 ggaaagggct actctctgcc cgagcctatc ccgctgctga acgctaaaca gattctggga
     3961 cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg
     4021 ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca
     4081 gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt
     4141 atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt
     4201 gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat
     4261 gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca
     4321 ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag
     4381 gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg
     4441 aatactctgg ccgctacgct gatccgagag ctgcgggcag ccctggaact gttcgagcag
     4501 gaaggcctgg ctccatatct gccacggtgg gagaagctgg ataacttcat caatagaccc
     4561 gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag
     4621 ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt
     4681 ctgcggtctg ccgaaaagct cgagGGAGGA GGAGGAAGCG GCGGAGGCGG AAGCGGAGGA
     4741 GGAGGAAGCC CAGAACACCA GCTCCTGTGC TGCGAAGTGG AAACCATCCG CCGCGCGTAC
     4801 CCCGATGCCA ACCTCCTCAA CGACCGGGTG CTGCGGGCCA TGCTGAAGGC GGAGGAGACC
     4861 TGCGCGCCCT CGGTGTCCTA CTTCAAATGT GTGCAGAAGG AGGTCCTGCC GTCCATGCGG
     4921 AAGATCGTCG CCACCTGGAT GCTGGAGGTC TGCGAGGAAC AGAAGTGCGA GGAGGAGGTC
     4981 TTCCCGCTGG CCATGAACTA CCTGGACCGC TTCCTGTCGC TGGAGCCCGT GAAAAAGAGC
     5041 CGCCTGCAGC TGCTGGGGGC CACTTGCATG TTCGTGGCCT CTAAGATGAA GGAGACCATC
     5101 CCCCTGACGG CCGAGAAGCT GTGCATCTAC ACCGACAACT CCATCCGGCC CGAGGAGCTG
     5161 CTGCAAATGG AGCTGCTCCT GGTGAACAAG CTCAAGTGGA ACCTGGCCGC AATGACCCCG
     5221 CACGATTTCA TTGAACACTT CCTCTCCAAA ATGCCAGAGG CGGAGGAGAA CAAACAGATC
     5281 ATCCGCAAAC ACGCGCAGAC CTTCGTTGCC CTCTGTGCCA CAGATGTGAA GTTCATTTCC
     5341 AATCCGCCCT CCATGGTGGC AGCGGGGAGC GTGGTGGCCG CAGTGCAAGG CCTGAACCTG
     5401 AGGAGCCCCA ACAACTTCCT GTCCTACTAC CGCCTCACAC GCTTCCTCTC CAGAGTGATC
     5461 AAGTGTGACC CGGACTGCCT CCGGGCCTGC CAGGAGCAGA TCGAAGCCCT GCTGGAGTCA
     5521 AGCCTGCGCC AGGCCCAGCA GAACATGGAC CCCAAGGCCG CCGAGGAGGA GGAAGAGGAG
     5581 GAGGAGGAGG TGGACCTGGC TTGCACACCC ACCGACGTGC GGGACGTGGA CATCTGAgtt
     5641 taaaccgctg agtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5701 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5761 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5821 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5881 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5941 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6001 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6061 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6121 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6181 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6241 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6301 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6361 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6421 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6481 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6541 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6601 gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     6661 tcgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     6721 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     6781 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     6841 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     6901 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6961 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     7021 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     7081 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     7141 cccctcactc ggggcgccag tcctccgatt gactgagtcg ccc
//