LOCUS       Exported                9066 bp ds-DNA     linear   UNK 01-JAN-1980
DEFINITION  (GB4326,GB_UD_32AB,GB0037)pDGB3_alpha2.
ACCESSION   GB_UA_7BE1
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 17, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9066
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    19..62
                     /label=repetition
     misc_feature    19..62
                     /label=repetition
     misc_feature    25..40
                     /label=MRE CUP2 mutated
                     /label=MRE\CUP2 mutated
     misc_feature    25..40
                     /label=MRE CUP2 mutated
                     /label=MRE\CUP2 mutated
     misc_feature    63..104
                     /label=repetition
     misc_feature    63..104
                     /label=repetition
     misc_feature    69..84
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    69..84
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    107..146
                     /label=repetition
     misc_feature    107..146
                     /label=repetition
     misc_feature    113..128
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    113..128
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    151..154
                     /label=No repite
     misc_feature    165..198
                     /label=repetition
     misc_feature    165..198
                     /label=repetition
     misc_feature    165..176
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    165..176
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    203..357
                     /label=misc_feature
     misc_feature    359..2197
     terminator      2198..2693
                     /label=Tnos
                     /note="/vntifkey=43"
     variation       2282
                     /label=T>C
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     misc_feature    2711..2735
                     /label=RB
                     /note="/vntifkey=21"
     misc_feature    complement(3797..4797)
                     /label=STA region from pVS1 plasmid
                     /label=STA\region\from\pVS1\plasmid
                     /note="STA region from pVS1 plasmid"
                     /note="/vntifkey=21"
     misc_feature    complement(5390..6390)
                     /label=pVS1-Rep ori
                     /label=pVS1-Rep\ori
                     /note="pVS1-REP; replication origin from pVS1"
                     /note="/vntifkey=21"
     misc_feature    complement(6800..7060)
                     /label=pBR322
                     /note="bom site from pBR322"
                     /note="/vntifkey=21"
     rep_origin      complement(7200..7480)
                     /direction=LEFT
                     /label=pBR322 ori
                     /label=pBR322\ori
                     /note="pBR322 origin of replication"
                     /note="/vntifkey=33"
     CDS             complement(7775..8587)
                     /label=KanR
                     /note="kanamycin resistance gene"
                     /note="/vntifkey=4"
     misc_feature    9020..9045
                     /label=LB
                     /note="/vntifkey=21"
ORIGIN
        1 ggagagtggt cttgggagag cgatgcgtct tttccgatga accgttccag caaaaaagac
       61 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaagcg atgcgtcttt
      121 tccgctgaac cgttccagca aaaaagacta ggagagcgat gcgtcttttc cgctgaaccg
      181 ttccagcaaa aaagactatc ccgtcctaat acctctacgt gcaagcagta taaaaacaaa
      241 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
      301 gtccagttac acttaaggtt aaatttgctg actctttttt acctttcatt tctgaaaaat
      361 ggacacgtat gccggcgctt atgatagaca atcaagagaa agagagaact cttctgccgc
      421 ctctccagct acacagagat ctgccaacga ggataaagct gctgatttac aaagagaggt
      481 agagagagat gggggtaggt ttagatttgt gggtcatttc tctgaggctc ctggtacatc
      541 agcctttggt actgctgaac ggcctgaatt tgaaagaata ttaaatgagt gtcgggccgg
      601 tcgactaaat atgataatag tttatgatgt atctaggttc agtagactca aagttatgga
      661 tgcaattccg atcgtgagcg agctacttgc actcggtgtt actatcgtaa gtacacaaga
      721 aggtgtattc aggcagggta atgtgatgga ccttatacac cttattatgc gactcgacgc
      781 tagccataaa gagtcatcac tgaagagtgc taaaatcttg gacaccaaga acctgcaaag
      841 ggagcttgga ggctatgtag gaggaaaagc tccgtatggt ttcgaactcg tgagtgagac
      901 aaaagagatt accagaaatg gtaggatggt gaacgtggtt attaataagc tagctcatag
      961 cacaacccct ctcacagggc cttttgagtt cgaacctgac gttattagat ggtggtggag
     1021 agagatcaaa actcacaaac atcttccatt taagccaggt agccaggctg ctatccaccc
     1081 tggatctatc actgggcttt gtaaaaggat ggatgctgac gcagtgccaa ctcgagggga
     1141 aacaatcggt aagaagactg catcttccgc ttgggatccc gctacagtta tgagaatcct
     1201 tcgggatccg cgtattgctg gattcgccgc agaagtcatt tataagaaaa aacctgatgg
     1261 tactcctact acaaaaatcg aaggttatag gatccaaagg gatcctatta ctttgcgtcc
     1321 agttgaatta gactgtggac cgatcatcga acctgccgag tggtacgagt tgcaggcctg
     1381 gcttgatggt agagggcgtg gaaagggttt atctcgggga caggcaattt tgtcagcaat
     1441 ggataagctt tattgtgagt gtggggctgt tatgacatca aaacgtggag aagagtcaat
     1501 caaggattct tacaggtgca gacgtcgtaa agtggtggac ccttctgctc caggacagca
     1561 tgagggtaca tgcaacgttt ctatggctgc tcttgataaa tttgtggccg aacgtatttt
     1621 caacaagatt agacacgctg aaggggatga ggaaacttta gctcttcttt gggaggctgc
     1681 ccggcgtttt ggtaagctaa cagaggcacc agaaaaaagt ggcgaaagag ctaatttggt
     1741 agccgaaaga gcagacgcct tgaatgcatt ggaagaactt tacgaagata gggctgccgg
     1801 agcatatgat ggaccagttg gtcggaagca tttcaggaag caacaagctg cactaacttt
     1861 gagacagcaa ggcgcagagg agcgactcgc agagcttgag gccgccgagg ccccaaagct
     1921 tccactcgat caatggtttc ctgaggatgc cgacgctgat cctacaggtc caaagagctg
     1981 gtggggaaga gctagcgtag acgataagag agtgtttgtg ggtctatttg ttgacaagat
     2041 tgtagttact aagtccacta cgggtcgagg acagggcact cccatcgaaa agagagccag
     2101 tattacctgg gcaaagcctc caaccgatga tgatgaagac gatgcacaag atggtaccga
     2161 ggatgtggca gcaccaaaaa aaaagaggaa ggtgtaagct tggaatggat cttcgatccc
     2221 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     2281 acgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     2341 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     2401 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcdc ggtgtcatct
     2461 atgttactag atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc tcgtgatacg
     2521 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2581 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2641 tccgctcatg agacaataac cctgataaat gcttcaataa tgggaccgac tcgcgcttga
     2701 gacgaagctt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     2761 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     2821 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
     2881 gtgcagtcgg cttctgacgt tcagtgcagc cgtcatctga aaacgacatg tcgcacaagt
     2941 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
     3001 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
     3061 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
     3121 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
     3181 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
     3241 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
     3301 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
     3361 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
     3421 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
     3481 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     3541 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     3601 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     3661 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     3721 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     3781 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacctctc
     3841 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     3901 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     3961 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatc cgatgagtaa ataaacaaat
     4021 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     4081 acgaccatcg gaacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     4141 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     4201 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     4261 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     4321 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     4381 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     4441 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     4501 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     4561 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     4621 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     4681 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     4741 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     4801 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     4861 ccgagctgct atctgaatag atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     4921 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     4981 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     5041 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg
     5101 tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa
     5161 gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga
     5221 atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg
     5281 tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat
     5341 gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct
     5401 gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt
     5461 agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat
     5521 ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc
     5581 cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg
     5641 cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc
     5701 catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc
     5761 cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat
     5821 cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac
     5881 ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc
     5941 acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag
     6001 tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa
     6061 tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat
     6121 gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat
     6181 gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggactctttc ctgtggatag
     6241 cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc
     6301 aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg
     6361 cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg
     6421 tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc
     6481 gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa
     6541 aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact
     6601 cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa
     6661 acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg gatgccggga
     6721 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     6781 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     6841 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6901 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6961 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7021 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7081 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7141 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7201 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7261 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7321 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7381 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7441 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7501 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     7561 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7621 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7681 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7741 ttaagggatt ttggtcatgc attctaggtg attagaaaaa ctcatcgagc atcaaatgaa
     7801 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     7861 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     7921 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     7981 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     8041 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     8101 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat acgcgatcgc
     8161 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     8221 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     8281 ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     8341 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     8401 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     8461 atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     8521 aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc cgttgaatat
     8581 ggctcataac agaacttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     8641 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     8701 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     8761 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     8821 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     8881 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     8941 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     9001 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacataaca agcttcgtct
     9061 cagtca
//
