LOCUS       Exported                8715 bp ds-DNA     linear   UNK 01-JAN-1980
DEFINITION  (GB4326,pUPD2_Flp,GB0037)pDGB3_alpha2.
ACCESSION   GB_UA_7BE0
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 17, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    19..62
                     /label=repetition
     misc_feature    19..62
                     /label=repetition
     misc_feature    25..40
                     /label=MRE CUP2 mutated
                     /label=MRE\CUP2 mutated
     misc_feature    25..40
                     /label=MRE CUP2 mutated
                     /label=MRE\CUP2 mutated
     misc_feature    63..104
                     /label=repetition
     misc_feature    63..104
                     /label=repetition
     misc_feature    69..84
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    69..84
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    107..146
                     /label=repetition
     misc_feature    107..146
                     /label=repetition
     misc_feature    113..128
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    113..128
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    151..154
                     /label=No repite
     misc_feature    165..198
                     /label=repetition
     misc_feature    165..198
                     /label=repetition
     misc_feature    165..176
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    165..176
                     /label=MRE CUP2
                     /label=MRE\CUP2
     misc_feature    203..357
                     /label=misc_feature
     misc_feature    359..1846
                     /label=FlpO
     misc_feature    1069..1257
                     /label=potato intron IV2
     terminator      1847..2342
                     /label=Tnos
                     /note="/vntifkey=43"
     variation       1931
                     /label=T>C
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     misc_feature    2360..2384
                     /label=RB
                     /note="/vntifkey=21"
     misc_feature    complement(3446..4446)
                     /label=STA region from pVS1 plasmid
                     /label=STA\region\from\pVS1\plasmid
                     /note="STA region from pVS1 plasmid"
                     /note="/vntifkey=21"
     misc_feature    complement(5039..6039)
                     /label=pVS1-Rep ori
                     /label=pVS1-Rep\ori
                     /note="pVS1-REP; replication origin from pVS1"
                     /note="/vntifkey=21"
     misc_feature    complement(6449..6709)
                     /label=pBR322
                     /note="bom site from pBR322"
                     /note="/vntifkey=21"
     rep_origin      complement(6849..7129)
                     /direction=LEFT
                     /label=pBR322 ori
                     /label=pBR322\ori
                     /note="pBR322 origin of replication"
                     /note="/vntifkey=33"
     CDS             complement(7424..8236)
                     /label=KanR
                     /note="kanamycin resistance gene"
                     /note="/vntifkey=4"
     misc_feature    8669..8694
                     /label=LB
                     /note="/vntifkey=21"
ORIGIN
        1 ggagagtggt cttgggagag cgatgcgtct tttccgatga accgttccag caaaaaagac
       61 taagcgatgc gtcttttccg ctgaaccgtt ccagcaaaaa agactaagcg atgcgtcttt
      121 tccgctgaac cgttccagca aaaaagacta ggagagcgat gcgtcttttc cgctgaaccg
      181 ttccagcaaa aaagactatc ccgtcctaat acctctacgt gcaagcagta taaaaacaaa
      241 atacataaac ataactaaaa tctcatatcg ttgtaattgt catatactag tggtaattaa
      301 gtccagttac acttaaggtt aaatttgctg actctttttt acctttcatt tctgaaaaat
      361 ggctcctaag aagaagagga aggtgatgag ccagttcgac atcctgtgca agaccccccc
      421 caaggtgctg gtgcggcagt tcgtggagag attcgagagg cccagcggcg agaagatcgc
      481 cagctgtgcc gccgagctga cctacctgtg ctggatgatc acccacaacg gcaccgccat
      541 caagagggcc accttcatga gctacaacac catcatcagc aacagcctga gcttcgacat
      601 cgtgaacaag agcctgcagt tcaagtacaa gacccagaag gccaccatcc tggaggccag
      661 cctgaagaag ctgatccccg cctgggagtt caccatcatc ccttacaacg gccagaagca
      721 ccagagcgac atcaccgaca tcgtgtccag cctgcagctg cagttcgaga gcagcgagga
      781 ggccgacaag ggcaacagcc acagcaagaa gatgctgaag gccctgctgt ccgagggcga
      841 gagcatctgg gagatcaccg agaagatcct gaacagcttc gagtacacca gcaggttcac
      901 caagaccaag accctgtacc agttcctgtt cctggccaca ttcatcaact gcggcaggtt
      961 cagcgacatc aagaacgtgg accccaagag cttcaagctg gtgcagaaca agtacctggg
     1021 cgtgatcatt cagtgcctgg tgaccgagac taagacaagc gtgtccaggt aagtttctgc
     1081 ttctaccttt gatatatata taataattat cattaattag tagtaatata atatttcaaa
     1141 tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta gtttataagt
     1201 gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga tgtgcaggca
     1261 catctacttt ttcagcgcca gaggcaggat cgaccccctg gtgtacctgg acgagttcct
     1321 gaggaacagc gagcccgtgc tgaagagagt gaacaggacc ggcaacagca gcagcaacaa
     1381 gcaggagtac cagctgctga aggacaacct ggtgcgcagc tacaacaagg ccctgaagaa
     1441 gaacgccccc taccccatct tcgctatcaa gaacggccct aagagccaca tcggcaggca
     1501 cctgatgacc agctttctga gcatgaaggg cctgaccgag ctgacaaacg tggtgggcaa
     1561 ctggagcgac aagagggcct ccgccgtggc caggaccacc tacacccacc agatcaccgc
     1621 catccccgac cactacttcg ccctggtgtc caggtactac gcctacgacc ccatcagcaa
     1681 ggagatgatc gccctgaagg acgagactaa ccccatcgag gagtggcagc acatcgagca
     1741 gctgaagggc agcgccgagg gcagcatcag ataccccgcc tggaacggca tcatcagcca
     1801 ggaggtgctg gactacctga gcagctacat caacaggcgg atctgagctt ggaatggatc
     1861 ttcgatcccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     1921 ggtcttgcga cgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     1981 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     2041 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcdcg
     2101 gtgtcatcta tgttactaga tcgggaattg ccaagctaat tcttgaagac gaaagggcct
     2161 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     2221 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2281 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat gggaccgact
     2341 cgcgcttgag acgaagcttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     2401 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     2461 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
     2521 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt
     2581 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     2641 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     2701 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     2761 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     2821 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     2881 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     2941 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     3001 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     3061 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
     3121 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     3181 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     3241 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     3301 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     3361 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     3421 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     3481 gcacctctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
     3541 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     3601 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatcc gatgagtaaa
     3661 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     3721 tcaggcaaga cgaccatcgg aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     3781 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     3841 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     3901 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     3961 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     4021 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     4081 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     4141 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     4201 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     4261 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     4321 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     4381 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     4441 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     4501 agaccattac cgagctgcta tctgaataga tcgcgcagct accagagtaa atgagcaaat
     4561 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     4621 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     4681 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     4741 gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     4801 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     4861 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     4921 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     4981 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     5041 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     5101 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     5161 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     5221 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     5281 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     5341 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     5401 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     5461 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     5521 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     5581 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     5641 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     5701 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     5761 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     5821 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gactctttcc
     5881 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     5941 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     6001 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     6061 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     6121 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     6181 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     6241 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     6301 acggtgaaaa cctctgacac atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg
     6361 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     6421 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     6481 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6541 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6601 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6661 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6721 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6781 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6841 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6901 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6961 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7021 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7081 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7141 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7201 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7261 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7321 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7381 aaactcacgt taagggattt tggtcatgca ttctaggtga ttagaaaaac tcatcgagca
     7441 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     7501 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     7561 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     7621 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     7681 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     7741 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agtcgaaata
     7801 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     7861 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     7921 ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     7981 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     8041 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     8101 tcccatacaa tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     8161 acccatataa atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc
     8221 gttgaatatg gctcataaca gaacttatta tttccttcct cttttctaca gtatttaaag
     8281 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
     8341 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
     8401 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
     8461 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
     8521 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     8581 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     8641 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacataacaa
     8701 gcttcgtctc agtca
//
