LOCUS       Exported                8940 bp ds-DNA     linear   UNK 01-JAN-1980
DEFINITION  (GB0030,GB4823,GB0037)pDGB3_alpha1.
ACCESSION   GB_UA_8843
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 17, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        5..1031
                     /label=35S
                     /note="/vntifkey=30"
     misc_feature    1033..2073
                     /label=NPGA
                     /note="/ApEinfo_fwdcolor=#d6b295"
                     /note="/ApEinfo_revcolor=#d6b295"
     CDS             1033..2073
                     /codon_start=1
                     /note="/ApEinfo_fwdcolor=#84b0dc"
                     /note="/ApEinfo_revcolor=#84b0dc"
                     /translation="MVQDTSSASTSPILTRWYIDTRPLTASTAALPLLETLQPADQISV
                     QKYYHLKDKHMSLASNLLKYLFVHRNCRIPWSSIVISRTPDPHRRPCYIPPSGSQEDSF
                     KDGYTGINVEFNVSHQASMVAIAGTAFTPNSGGDSKLKPEVGIDITCVNERQGRNGEER
                     SLESLRQYIDIFSEVFSTAEMANIRRLDGVSSSSLSADRLVDYGYRLFYTYWALKEAYI
                     KMTGEALLAPWLRELEFSNVVAPAAVAESGDSAGDFGEPYTGVRTTLYKNLVEDVRIEV
                     AALGGDYLFATAARGGGIGASSRPGGGPDGSGIRSQDPWRPFKKLDIERDIQPCATGVC
                     NCLSRG"
     misc_feature    1527
                     /label=T>A
                     /note="/ApEinfo_fwdcolor=#f58a5e"
                     /note="/ApEinfo_revcolor=#f58a5e"
     terminator      2074..2569
                     /label=Tnos
                     /note="/vntifkey=43"
     variation       2158
                     /label=T>C
                     /label=nonstandard type:  mutation
                     /note="/vntifkey=62"
     misc_feature    2570..2573
                     /label=CGCT
                     /note="/vntifkey=21"
     misc_feature    2574..2577
                     /label=GTCA
                     /note="/vntifkey=21"
     misc_feature    2590..2614
                     /label=RB
                     /note="/vntifkey=21"
     misc_feature    complement(3676..4676)
                     /label=STA region from pVS1 plasmid
                     /label=STA\region\from\pVS1\plasmid
                     /note="STA region from pVS1 plasmid"
                     /note="/vntifkey=21"
     misc_feature    complement(5269..6269)
                     /label=pVS1-Rep ori
                     /label=pVS1-Rep\ori
                     /note="pVS1-REP; replication origin from pVS1"
                     /note="/vntifkey=21"
     misc_feature    complement(6679..6939)
                     /label=pBR322
                     /note="bom site from pBR322"
                     /note="/vntifkey=21"
     rep_origin      complement(7079..7359)
                     /direction=LEFT
                     /label=pBR322 ori
                     /label=pBR322\ori
                     /note="pBR322 origin of replication"
                     /note="/vntifkey=33"
     CDS             complement(7654..8466)
                     /codon_start=1
                     /label=KanR
                     /note="kanamycin resistance gene"
                     /note="/vntifkey=4"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    8899..8924
                     /label=LB
                     /note="/vntifkey=21"
ORIGIN
        1 ggagactaga gccaagctga tctcctttgc cccggagatc accatggacg actttctcta
       61 tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt tcaccaccga
      121 taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga tggttagaga
      181 ggcctacgcg gcaggtctga tcaagacgat ctacccgagt aataatctcc aggagatcaa
      241 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      301 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      361 cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag tagttcctac
      421 tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac tcgccgtgaa
      481 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
      541 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      601 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      661 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      721 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      781 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      841 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
      901 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
      961 tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt acaatttact
     1021 attctagtcg aaatggtcca agacacctca agcgctagta ccagtccaat tttgacaaga
     1081 tggtatatag atactagacc tttgactgct tctaccgcag ccttgccact gttggaaact
     1141 ctgcagcctg cagaccagat aagtgtccaa aagtactacc atctgaagga taagcatatg
     1201 tctctcgcat ccaatcttct caagtacttg tttgttcaca ggaattgccg aatcccatgg
     1261 tctagcattg taatatcaag aactccagat ccccatagaa ggccctgtta tataccaccc
     1321 tctggttctc aagaggacag ctttaaggat ggctacactg gaattaatgt cgagttcaac
     1381 gtatcccatc aagcaagcat ggtggcaata gcaggcactg catttacacc caactctgga
     1441 ggtgattcca aacttaagcc agaggttggc atagacatta cttgcgtgaa tgagcgacag
     1501 gggcgaaacg gtgaggagcg atccctagaa agtttgaggc agtacataga tattttctca
     1561 gaggtttttt ccaccgcaga gatggcaaac atcagaagat tggatggggt gtcatcaagc
     1621 tccttgagcg ccgataggtt ggtggactat ggttaccgat tgttttacac ttattgggct
     1681 cttaaggaag catacatcaa gatgacaggg gaagccctgc tggcaccatg gctcagggag
     1741 ttggaattta gtaatgtcgt tgccccagca gcagtggccg aatccggaga ttccgccgga
     1801 gactttggag agccttatac cggggtacga acaacccttt acaaaaactt ggtcgaggat
     1861 gttaggatag aggtggctgc tctgggaggt gactacttgt tcgccactgc tgcacgaggg
     1921 ggcggtatag gtgcctccag ccgaccaggg ggaggccctg atggctcagg aattaggagt
     1981 caagatccct ggcgaccatt taaaaagctc gacatagaga gagacataca gccttgcgcc
     2041 actggagtct gtaattgtct gtctcgaggt tgagcttgga atggatcttc gatcccgatc
     2101 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgacga
     2161 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     2221 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     2281 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcdcggtg tcatctatgt
     2341 tactagatcg ggaattgcca agctaattct tgaagacgaa agggcctcgt gatacgccta
     2401 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     2461 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2521 ctcatgagac aataaccctg ataaatgctt caataatggg accgactcgc gctgtcatga
     2581 gacgaattct gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2641 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     2701 ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag
     2761 tgcagtcggc ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc
     2821 ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt
     2881 tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag
     2941 cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa
     3001 ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg
     3061 caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt
     3121 tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc
     3181 cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac
     3241 caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg
     3301 ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa
     3361 gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga
     3421 ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct
     3481 gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc
     3541 cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca
     3601 agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa
     3661 gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacctctca
     3721 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     3781 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     3841 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatcc gatgagtaaa taaacaaata
     3901 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     3961 cgaccatcgg aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     4021 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     4081 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     4141 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     4201 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     4261 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     4321 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     4381 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     4441 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     4501 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     4561 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     4621 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     4681 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     4741 cgagctgcta tctgaataga tcgcgcagct accagagtaa atgagcaaat gaataaatga
     4801 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     4861 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     4921 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt
     4981 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     5041 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     5101 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     5161 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     5221 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     5281 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     5341 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     5401 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     5461 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     5521 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     5581 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     5641 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     5701 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     5761 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     5821 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     5881 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     5941 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     6001 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     6061 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gactctttcc tgtggatagc
     6121 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     6181 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     6241 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     6301 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     6361 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     6421 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     6481 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     6541 cctctgacac atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg atgccgggag
     6601 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     6661 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     6721 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6781 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6841 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6961 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7021 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7081 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7141 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7201 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7261 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7321 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7381 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7441 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7501 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7561 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7621 taagggattt tggtcatgca ttctaggtga ttagaaaaac tcatcgagca tcaaatgaaa
     7681 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     7741 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     7801 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     7861 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     7921 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     7981 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agtcgaaata cgcgatcgct
     8041 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     8101 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     8161 tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     8221 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     8281 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     8341 tcggtagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     8401 atcagcatcc atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg
     8461 gctcataaca gaacttatta tttccttcct cttttctaca gtatttaaag ataccccaag
     8521 aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     8581 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     8641 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     8701 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     8761 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     8821 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     8881 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacataacga attcgtctca
//
