LOCUS       pMVV098_(BBR1_PhlFAM_+_ 4720 bp ds-DNA     circular     27-JUN-2023
DEFINITION  .
KEYWORDS    "creator:Brian Pfleger Group" "marker:KanR"
FEATURES             Location/Qualifiers
     CDS             complement(39..854)
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-Ia note: confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes product: aminoglycoside phosphotransferase transl_table: 1 translation: MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     misc_feature    955..1007
                     /label="Terminator"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    1027..1092
                     /label="PPhIF Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: Negatively regulated by PhlFAM"
     misc_RNA        1095..1144
                     /label="sTRSV HHRz"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
                     /note="note: hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)"
     misc_feature    1168..1193
                     /label="RBS"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     CDS             1194..1907
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: sfGFP transl_table: 1 translation: MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     misc_feature    1908..1910
                     /label="STOP"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    1911..1971
                     /label="Terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1976..2029
                     /label="PlacIQ Promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2030..2057
                     /label="phl2 RBS"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     misc_feature    2058..2660
                     /label="PhlFAM (DAPG sensor protein)"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2670..2924
                     /label="Terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(3236..3898)
                     /label="pBBR1 Rep"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 experiment: gene: note: product: replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica protein_id: transl_table: 1 translation: MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLHHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR*"
     rep_origin      3899..4670
                     /label="pBBR1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication"
ORIGIN
        1 ctaaagtata tatgagtaaa cttggtctga cagttacctt agaaaaactc atcgagcatc
       61 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      121 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      181 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      241 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      301 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      361 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      421 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      481 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      541 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      601 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      661 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
      721 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
      781 ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt
      841 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
      901 catgatgata tatttttatc ttgtgcaatg tacatcagag attttgagac acaaccaatt
      961 attgaaggcc tccctaacgg ggggcctttt tttgtttctg gtctcccgct taacgatcgt
     1021 tggctgcgac gtacggtgga atctgattcg ttaccaattg acatgatacg aaacgtaccg
     1081 tatcgttaag gtagctgtca ccggatgtgc tttccggtct gatgagtccg tgaggacgaa
     1141 acagcctcta caaataattt tgtttaatac tagagaaaga ggggaaatac tagATGAGCA
     1201 AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT GGTGATGTTA
     1261 ATGGGCACAA ATTTTCTGTC CGTGGAGAGG GTGAAGGTGA TGCTACAAAC GGAAAACTCA
     1321 CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC GTGGCCAACA CTTGTCACTA
     1381 CTCTGACCTA TGGTGTTCAA TGCTTTTCCC GTTATCCGGA TCACATGAAA CGGCATGACT
     1441 TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAACG CACTATATCT TTCAAAGATG
     1501 ACGGGACCTA CAAGACGCGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT GTTAATCGTA
     1561 TCGAGTTAAA GGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTTGGACAC AAACTTGAGT
     1621 ACAACTTTAA CTCACACAAT GTATACATCA CGGCAGACAA ACAAAAGAAT GGAATCAAAG
     1681 CTAACTTCAA AATTCGCCAC AACGTTGAAG ATGGTTCCGT TCAACTAGCA GACCATTATC
     1741 AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCGA
     1801 CACAATCTGT CCTTTCGAAA GATCCCAACG AAAAGCGTGA CCACATGGTC CTTCTTGAGT
     1861 TTGTAACTGC TGCTGGGATT ACACATggca tggatgagct ctacaaataa ctcggtacca
     1921 aattccagaa aagaggcctc ccgaaagggg ggcctttttt cgttttggtc caatggcggc
     1981 gcgccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
     2041 aattcatggg ggtgaatatg gcacgtaccc cgagccgtag cagcattggt agcctgcgta
     2101 gtccgcatac ccataaagca attctgacca gcaccattga aatcctgaaa gaatgtggtt
     2161 atagcggtct gagcattgaa agcgtggcac gtcgcgccgg tgcaggcaaa ccgaccattt
     2221 atcgttggtg gaccaacaaa gcagcactga ttgccgaagt gtatgaaaat gaaatcgaac
     2281 aggtacgtaa atttccggat ttgggtagct ttaaagccga tctggatttt ctgctgcata
     2341 atctgtggaa agtttggcgt gaaaccattt gtggtgaagc atttcgttgt gttattgcag
     2401 aagcacagtt ggaccctgta accctgaccc aactgaaaga tcagtttatg gaacgtcgtc
     2461 gtgagatacc gaaaaaactg gttgaagatg ccattagcaa tggtgaactg ccgaaagata
     2521 tcaatcgtga actgctgctg gatatgattt ttggtttttg ttggtatcgc ctgctgaccg
     2581 aacagttgac cgttgaacag gatattgaag aatttacctt cctgctgatt aatggtgttt
     2641 gtccgggtac acagtgttga taaggatcct aattggtaac gaatcagaca attgacggct
     2701 cgagggagta gcatagggtt tgcagaatcc ctgcttcgtc catttgacag gcacattatg
     2761 catcgatgat aagctgtcaa acatgagcag atcctctacg ccggacgcat cgtggccggc
     2821 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     2881 gatcgggctc gccacttcgg gctcatgagc aaatatttta tctgaggtgc ttcctcgctc
     2941 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
     3001 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
     3061 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
     3121 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
     3181 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgttctacc
     3241 ggcgcggcag cgtgacccgt gtcggcggct ccaacggctc gccatcgTCC AGAAAACACG
     3301 GCTCATCggg catcggcagg cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca
     3361 aggctggcag gtctggttcc atgcccggaa tgccgggctg gctgggcggc tcctcgccgg
     3421 ggccggtcgg tagttgctgc tcgcccggat acagggtcgg gatgcggcgc aggtcgccat
     3481 gccccaacag cgattcgtcc tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg
     3541 acaggcgcaa ctggtcgcgg ggctggcccc acgccacgcg gtcattgacc acgtaggccg
     3601 acacggtgcc ggggccgttg agcttcacga cggagatcca gcgctcggcc accaagtcct
     3661 tgactgcgta ttggaccgtc cgcaaagaac gtccgatgag cttggaaagt gtcttctggc
     3721 tgaccaccac ggcgttctgg tggcccatct gcgccacgag gtgatgcagc agcattgccg
     3781 ccgtgggttt cctcgcaata agcccggccc acgcctcatg cgctttgcgt tccgtttgca
     3841 cccagtgacc gggcttgttc ttggcttgaa tgccgatttc tctggactgc gtggccatgc
     3901 ttatctccat gcggtagggg tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact
     3961 tttcggcagc gcgacaacaa ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc
     4021 tttaacctac gcaatgagct attgcggggg gtgccgcaat gagctgttgc gtacccccct
     4081 tttttaagtt gttgattttt aagtctttcg catttcgccc tatatctagt tctttggtgc
     4141 ccaaagaagg gcacccctgc ggggttcccc cacgccttcg gcgcggctcc ccctccggca
     4201 aaaagtggcc cctccggggc ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg
     4261 gcgctgcccc cttggaaccc ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg
     4321 gcttcgccct tcgactgccc ccactcgcat aggcttgggt cgttccaggc gcgtcaaggc
     4381 caagccgctg cgcggtcgct gcgcgagcct tgacccgcct tccacttggt gtccaaccgg
     4441 caagcgaagc gcgcaggccg caggccggag gcttttcccc agagaaaatt aaaaaaattg
     4501 atggggcaag gccgcaggcc gcgcagttgg agccggtggg tatgtggtcg aaggctgggt
     4561 agccggtggg caatccctgt ggtcaagctc gtgggcaggc gcagcctgtc catcagcttg
     4621 tccagcaggg ttgtccacgg gccgagcgaa gcgagccagc cggtggccgc gaagatcatc
     4681 ttattaaggg gtctgacgct cagtggaacg aaaaatcaat
//