LOCUS       pMVV101_(BBR1_VanRAM_+_ 4837 bp ds-DNA     circular     27-JUN-2023
DEFINITION  .
KEYWORDS    "creator:Brian Pfleger Group" "marker:KanR"
FEATURES             Location/Qualifiers
     misc_RNA        1..50
                     /label="sTRSV HHRz"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
                     /note="note: hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)"
     CDS             100..813
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: sfGFP transl_table: 1 translation: MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK"
     misc_feature    814..816
                     /label="STOP"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    817..877
                     /label="Terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    882..935
                     /label="lacIQ Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    936..964
                     /label="van2 RBS"
                     /ApEinfo_revcolor="#339966"
                     /ApEinfo_fwdcolor="#339966"
     CDS             965..1699
                     /label="vanRAM sensor protein"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
                     /note="codon_start: 1 note: pVanCC repression relieved upon addition of vanillic acid transl_table: 1 translation: MDMPRIKPGQRVMMALRKMIASGEIKSGERIAEIPTAAALGVSRMPVRIALRSLEQEGLVVRLGARGYAARGVSSDQIRDAIEVRGVLEGFAARRLAERGMTAETHARFVVLIAEGEALFAAGRLNGEDLDRYAAYNQAFHDTLVSAAGNGAVESALARNGFEPFAAAGALALDLMDLSAEYEHLLAAHRQHQAVLDAVSCGDAEGAERIMRDHALAAIRNAKVFEAAASAGAPLGAAWSIRAD*"
     misc_feature    1709..1963
                     /label="Terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(2275..2937)
                     /label="pBBR1 Rep"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 experiment: gene: note: product: replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica protein_id: transl_table: 1 translation: MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLHHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR*"
     rep_origin      2938..3709
                     /label="pBBR1 oriV"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication"
     CDS             complement(3798..4613)
                     /label="KanR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(3')-Ia note: confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes product: aminoglycoside phosphotransferase transl_table: 1 translation: MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     misc_feature    4714..4766
                     /label="Terminator"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    4786..4835
                     /label="PVanCC Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: repression relieved upon addition of vanillic acid"
ORIGIN
        1 ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag cctctacaaa
       61 taattttgtt taatactaga gaaagagggg aaatactagA TGAGCAAAGG AGAAGAACTT
      121 TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT
      181 TCTGTCCGTG GAGAGGGTGA AGGTGATGCT ACAAACGGAA AACTCACCCT TAAATTTATT
      241 TGCACTACTG GAAAACTACC TGTTCCGTGG CCAACACTTG TCACTACTCT GACCTATGGT
      301 GTTCAATGCT TTTCCCGTTA TCCGGATCAC ATGAAACGGC ATGACTTTTT CAAGAGTGCC
      361 ATGCCCGAAG GTTATGTACA GGAACGCACT ATATCTTTCA AAGATGACGG GACCTACAAG
      421 ACGCGTGCTG AAGTCAAGTT TGAAGGTGAT ACCCTTGTTA ATCGTATCGA GTTAAAGGGT
      481 ATTGATTTTA AAGAAGATGG AAACATTCTT GGACACAAAC TTGAGTACAA CTTTAACTCA
      541 CACAATGTAT ACATCACGGC AGACAAACAA AAGAATGGAA TCAAAGCTAA CTTCAAAATT
      601 CGCCACAACG TTGAAGATGG TTCCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA
      661 ATTGGCGATG GCCCTGTCCT TTTACCAGAC AACCATTACC TGTCGACACA ATCTGTCCTT
      721 TCGAAAGATC CCAACGAAAA GCGTGACCAC ATGGTCCTTC TTGAGTTTGT AACTGCTGCT
      781 GGGATTACAC ATggcatgga tgagctctac aaataactcg gtaccaaatt ccagaaaaga
      841 ggcctcccga aaggggggcc ttttttcgtt ttggtccaat ggcggcgcgc catcgaatgg
      901 tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt caggggggtg
      961 aataatggac atgcctcgta ttaaaccggg tcagcgtgtt atgatggcac tgcgtaaaat
     1021 gattgcaagc ggtgaaatca aaagtggtga acgtattgca gaaattccga ccgcagcagc
     1081 actgggtgtt agccgtatgc cggttcgtat cgcactgcgt tcactggaac aagaaggtct
     1141 ggttgttcgt ctgggtgcac gtggttatgc agcccgtggt gttagcagcg atcagattcg
     1201 tgatgcaatt gaagttcgtg gtgttctgga aggttttgca gcacgtcgtc tggcagaacg
     1261 tggtatgacc gcagaaaccc atgcacgttt tgttgtactg attgcagaag gtgaagcact
     1321 gtttgcagcc ggtcgcctga atggtgaaga tctggatcgt tatgccgcat ataatcaggc
     1381 atttcatgat accctggtta gcgcagcagg taatggtgca gttgaaagcg cactggcacg
     1441 taatggtttt gaaccgtttg cagcagccgg tgcactggcc ctggatctga tggacctgtc
     1501 tgccgaatat gaacatctgc tggcagcaca tcgtcagcat caggcagttc tggatgcagt
     1561 tagctgtggt gatgccgaag gtgcagaacg tattatgcgt gatcatgcac tggcagcaat
     1621 tcgtaatgca aaagtttttg aagcagcagc aagcgcaggc gcaccgctgg gtgcagcatg
     1681 gtcaattcgt gcagattgat aaggatccta attggtaacg aatcagacaa ttgacggctc
     1741 gagggagtag catagggttt gcagaatccc tgcttcgtcc atttgacagg cacattatgc
     1801 atcgatgata agctgtcaaa catgagcaga tcctctacgc cagacgcatc gtggccggca
     1861 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     1921 atcgggctcg ccacttcggg ctcatgagca aatattttat ctgaggtgct tcctcgctca
     1981 ctgactcgct gcacgaggca gacctcagcg ctagcggagt gtatactggc ttactatgtt
     2041 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     2101 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     2161 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     2221 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgttctaccg
     2281 gcgcggcagc gtgacccgtg tcggcggctc caacggctcg ccatcgTCCA GAAAACACGG
     2341 CTCATCgggc atcggcaggc gctgctgccc gcgccgttcc cattcctccg tttcggtcaa
     2401 ggctggcagg tctggttcca tgcccggaat gccgggctgg ctgggcggct cctcgccggg
     2461 gccggtcggt agttgctgct cgcccggata cagggtcggg atgcggcgca ggtcgccatg
     2521 ccccaacagc gattcgtcct ggtcgtcgtg atcaaccacc acggcggcac tgaacaccga
     2581 caggcgcaac tggtcgcggg gctggcccca cgccacgcgg tcattgacca cgtaggccga
     2641 cacggtgccg gggccgttga gcttcacgac ggagatccag cgctcggcca ccaagtcctt
     2701 gactgcgtat tggaccgtcc gcaaagaacg tccgatgagc ttggaaagtg tcttctggct
     2761 gaccaccacg gcgttctggt ggcccatctg cgccacgagg tgatgcagca gcattgccgc
     2821 cgtgggtttc ctcgcaataa gcccggccca cgcctcatgc gctttgcgtt ccgtttgcac
     2881 ccagtgaccg ggcttgttct tggcttgaat gccgatttct ctggactgcg tggccatgct
     2941 tatctccatg cggtaggggt gccgcacggt tgcggcacca tgcgcaatca gctgcaactt
     3001 ttcggcagcg cgacaacaat tatgcgttgc gtaaaagtgg cagtcaatta cagattttct
     3061 ttaacctacg caatgagcta ttgcgggggg tgccgcaatg agctgttgcg tacccccctt
     3121 ttttaagttg ttgattttta agtctttcgc atttcgccct atatctagtt ctttggtgcc
     3181 caaagaaggg cacccctgcg gggttccccc acgccttcgg cgcggctccc cctccggcaa
     3241 aaagtggccc ctccggggct tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg
     3301 cgctgccccc ttggaacccc cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg
     3361 cttcgccctt cgactgcccc cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc
     3421 aagccgctgc gcggtcgctg cgcgagcctt gacccgcctt ccacttggtg tccaaccggc
     3481 aagcgaagcg cgcaggccgc aggccggagg cttttcccca gagaaaatta aaaaaattga
     3541 tggggcaagg ccgcaggccg cgcagttgga gccggtgggt atgtggtcga aggctgggta
     3601 gccggtgggc aatccctgtg gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt
     3661 ccagcagggt tgtccacggg ccgagcgaag cgagccagcc ggtggccgcg aagatcatct
     3721 tattaagggg tctgacgctc agtggaacga aaaatcaatc taaagtatat atgagtaaac
     3781 ttggtctgac agttacctta gaaaaactca tcgagcatca aatgaaactg caatttattc
     3841 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     3901 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     3961 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     4021 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     4081 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     4141 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     4201 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     4261 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     4321 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     4381 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     4441 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     4501 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     4561 ttggaattta atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc
     4621 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     4681 tgtgcaatgt acatcagaga ttttgagaca caaccaatta ttgaaggcct ccctaacggg
     4741 gggccttttt ttgtttctgg tctcccgctt aacgatcgtt ggctgattgg atccaattga
     4801 cagctagctc agtcctaggt accattggat ccaatag
//