LOCUS       pMRXip-TMEM192-m        7617 bp DNA     circular SYN 18-JAN-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 24, 2024 from SnapGene Viewer 7.1.0
            https://www.snapgene.com
COMMENT     ID   ; SV ; circular; genomic DNA; STD; SYN; 8176 BP.
            XX
            AC   ;
            XX
            DT   24-DEC-2019 (Rel. 1, Created)
            DT   24-DEC-2019 (Rel. 1, Last updated, Version 1)
            XX
            DE   synthetic circular DNA
            XX
            KW   .
            XX
            OS   synthetic DNA construct
            OC   .
            XX
            RN   [1]
            RP   1 to 8176
            RA   .
            RT   ""Direct Submission""
            RL   Exported Saturday, Jan 18, 2020 from SnapGene Viewer 5.0.6
            RL   https://www.snapgene.com
            XX
            CC   GateWay(tm) LR reaction between #37 pENTR1A-Ct-Lamp1.xdna as an
            Entry
            CC   vector and pMRXip2c-CtmCherry.xdna as a Destination vector.
            FH   Key             Location/Qualifiers
            FH
            XX
FEATURES             Location/Qualifiers
     source          1..7617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        183..562
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #5ac3fa"
     misc_feature    921..1278
                     /locus_tag="MMLV Psi"
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia 
                     virus(MMLV)"
                     /note="color: #7eff74"
     CDS             1343..1759
                     /locus_tag="gag (truncated)"
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) 
                     gaggene lacking the start codon"
                     /note="color: #e9d024; direction: RIGHT"
     misc_feature    1769..2143
                     /locus_tag="pol region"
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
                     /note="color: #7eff74"
     CDS             2173..2985
                     /codon_start=1
                     /label=TMEM192
                     /note="color: #993366"
                     /translation="MAAGGRMEDGSLDITQSIEDDPLLDAQLLPHHSLQAHFRPRFHPL
                     PTVIIVNLLWFIHLVFVVLAFLTGVLCSYPNPNEDKCPGNYTNPLKVQTVIILGKVILW
                     ILHLLLECYIQYHHSKIRNRGYNLIYRSTRHLKRLALMIQSSGNTVLLLILCMQHSFPE
                     PGRLYLDLILAILALELICSLICLLIYTVKIRRFNKAKPEPDILEEEKIYAYPSNITSE
                     TGFRTISSLEEIVEKQGDTIEYLKRHNALLSKRLLALTSSDLGCQPSRT"
     regulatory      3031..3040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             join(3037..3039,3040..3042,3043..3705)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /note="This feature has 3 segments:
                        1: 3037 .. 3039 / #ff0000
                        2: 3040 .. 3042 / #ff0000 / 1a
                        3: 3043 .. 3705 / #ff0000"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     misc_feature    3737..4289
                     /locus_tag="IRES"
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #7eff74"
     CDS             4301..4900
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /locus_tag="PuroR"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #e9d024"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             5124..5593
                     /locus_tag="long terminal repeat from Moloney 
                     murineleukemia  virus"
                     /label=long terminal repeat from Moloney murine
                     leukem...
                     /label=long terminal repeat from Moloney murine leukemia
                     virus
                     /note="long terminal repeat from Moloney murine 
                     leukemiavirus"
                     /note="color: #86f71d"
     rep_origin      complement(5894..6482)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #999999"
     CDS             complement(6646..7506)
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="AmpR"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,and 
                     related antibiotics"
                     /note="color: #e9d024"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7507..7611)
                     /gene="bla"
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="color: #346ee0; direction: LEFT"
ORIGIN
        1 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
       61 aaataggcgt atcacgaggc cctttcgtcc ttcactcgag gagcttggcc cattgcatac
      121 gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat taccgccatg
      181 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      241 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      301 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      361 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      421 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      481 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      541 attagtcatc gctattacca tgctagagaa ccatcagatg tttccagggt gccccaagga
      601 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
      661 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag
      721 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc
      781 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt
      841 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca
      901 ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc cgattgtcta
      961 gtgtctatga ctgattttat gcgcctgcgt cggtactagt tagctaacta gctctgtatc
     1021 tggcggaccc gtggtggaac tgacgagttc ggaacacccg gccgcaaccc tgggagacgt
     1081 cccagggact tcgggggccg tttttgtggc ccgacctgag tccaaaaatc ccgatcgttt
     1141 tggactcttt ggtgcacccc ccttagagga gggatatgtg gttctggtag gagacgagaa
     1201 cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg gtttgggacc gaagccgcgc
     1261 cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta
     1321 tttgtctgaa aatatgggcc cgggccagac tgttaccact cccttaagtt tgaccttagg
     1381 tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca agaagagacg
     1441 ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc cgcgagacgg
     1501 cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac ctggcccgca
     1561 tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt ttgacccccc
     1621 tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc catccgcccc
     1681 gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt atccagccct
     1741 cactccttct ctaggcgccc ccatatggcc atatgagatc ttatatgggg cacccccgcc
     1801 ccttgtaaac ttccctgacc ctgacatgac aagagttact aacagcccct ctctccaagc
     1861 tcacttacag gctctctact tagtccagca cgaagtctgg agacctctgg cggcagccta
     1921 ccaagaacaa ctggaccgac cggtggtacc tcacccttac cgagtcggcg acacagtgtg
     1981 ggtccgccga caccagacta agaacctaga acctcgctgg aaaggacctt acacagtcct
     2041 gctgaccacc cccaccgccc tcaaagtaga cggcatcgca gcttggatac acgccgccca
     2101 cgtgaaggct gccgaccccg ggggtggacc atcctctaga ctgccggatc ccagtgtggt
     2161 ggtacgggaa ttatggcggc ggggggcagg atggaggacg gttccttgga tatcacccag
     2221 agtattgaag acgacccact tctggatgcc cagcttctcc cacaccactc attacaagct
     2281 cactttagac cccgattcca tcctcttcct acagtcatca tagtgaatct tctgtggttt
     2341 attcatctcg tgtttgttgt tttagcattt ttaacaggtg tgctttgttc ttatcctaat
     2401 ccaaatgagg acaagtgccc aggaaattac acaaacccat tgaaagttca gacggttata
     2461 atccttggga aagttatttt gtggattctc catttactcc ttgaatgcta catccagtat
     2521 caccacagca aaatcagaaa ccgaggctat aacttgatct accgatcaac aaggcatctc
     2581 aagagacttg cgttgatgat acagtcctct ggcaacacag tgcttctcct catactgtgc
     2641 atgcagcact ccttcccaga gcctggcaga ttgtatcttg acctcattct ggccatcttg
     2701 gcactggaac tcatctgttc cctgatatgt ctcctcattt acacagtgaa aatccggaga
     2761 tttaataaag ctaaaccaga gcctgatata cttgaagaag aaaaaatcta tgcttacccc
     2821 agcaatatta cctcggagac tggattcaga actatttcaa gcctagaaga aattgttgaa
     2881 aagcaaggag acaccattga atacctgaag cgacacaatg cgctgctgag taagcgattg
     2941 ttggctctca cttcctcaga cctgggctgt cagccaagta gaacgggaat tctgcagtcg
     3001 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagtgtgat cgctaaacaa
     3061 atgacctaca aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc
     3121 gatggaaaag gaaagcctta cgagggagag cagacagtaa agctcactgt caccaagggt
     3181 ggacctctgc catttgcttg ggatatttta tcaccacagc ttcagtacgg aagcatacca
     3241 ttcaccaagt accctgaaga catccctgat tatttcaagc agtcattccc tgagggatat
     3301 acatgggaga ggagcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc
     3361 agcatccaag gcaactgttt catctacaat gtcaaaatct ctggtgagaa ctttcctccc
     3421 aatggacctg ttatgcagaa gaagacacag ggctgggaac ccagcactga gcgtctcttt
     3481 gcacgagatg gaatgctgat aggaaacgat tatatggctc tgaagttgga aggaggtggt
     3541 cactatttgt gtgaatttaa atctacttac aaggcaaaga agcctgtgag gatgccaggg
     3601 cgccacgaga ttgaccgcaa actggatgta accagtcaca acagggatta cacatctgtt
     3661 gagcagtgtg aaatagccat tgcacgccac tctttgctcg gttaaggccg ctacgtaaat
     3721 tccgcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     3781 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     3841 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     3901 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     3961 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     4021 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     4081 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     4141 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     4201 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     4261 gttttccttt gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac
     4321 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     4381 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     4441 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4501 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4561 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4621 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4681 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4741 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4801 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4861 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     4921 gcgcccgacc gaaaggagcg cacgacccca tggctcgacg ataaaataaa agattttatt
     4981 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     5041 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc
     5101 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     5161 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
     5221 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     5281 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     5341 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     5401 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
     5461 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     5521 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     5581 cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtatta
     5641 acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5701 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5761 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5821 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5881 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5941 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6001 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6061 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6121 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6181 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6241 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6301 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6361 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6421 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6481 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6541 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgg
     6601 ccgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6661 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6721 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6781 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6841 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6901 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6961 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7021 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7081 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7141 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7201 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7261 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7321 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7381 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7441 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7501 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7561 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatt
//
