LOCUS       pMRXib-TMEM192-m        7400 bp DNA     circular SYN 31-JUL-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 24, 2024 from SnapGene Viewer 7.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    22..598
                     /label=IRES
                     /note="color: #a6acb3"
     misc_feature    602..1030
                     /label=BsrR
                     /note="color: #0000ff"
     LTR             1203..1672
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
                     /note="color: #ffe4c4"
     rep_origin      complement(1973..2561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(2725..3516,3517..3585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2725 .. 3516 / #ccffcc
                        2: 3517 .. 3585 / #ccffcc / signal sequence
                     Cleavage site after base 3516"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3586..3690)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     enhancer        3878..4257
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     misc_feature    4616..4973
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
                     /note="color: #ffe4c4"
     CDS             5038..5454
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
                     /note="color: #993366; direction: RIGHT"
     misc_feature    5464..5838
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
                     /note="color: #ffe4c4
                     Cleavage site after base 5818"
     CDS             5868..6680
                     /codon_start=1
                     /label=TMEM192
                     /note="color: #993366"
                     /translation="MAAGGRMEDGSLDITQSIEDDPLLDAQLLPHHSLQAHFRPRFHPL
                     PTVIIVNLLWFIHLVFVVLAFLTGVLCSYPNPNEDKCPGNYTNPLKVQTVIILGKVILW
                     ILHLLLECYIQYHHSKIRNRGYNLIYRSTRHLKRLALMIQSSGNTVLLLILCMQHSFPE
                     PGRLYLDLILAILALELICSLICLLIYTVKIRRFNKAKPEPDILEEEKIYAYPSNITSE
                     TGFRTISSLEEIVEKQGDTIEYLKRHNALLSKRLLALTSSDLGCQPSRT"
     regulatory      6726..6735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             join(6732..6734,6735..6737,6738..7400)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /note="This feature has 3 segments:
                        1: 6732 .. 6734 / #ff0000
                        2: 6735 .. 6737 / #ff0000 / 1a
                        3: 6738 .. 7400 / #ff0000"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
ORIGIN
        1 ggccgcaacg gtttccctct agcgggatca attccgcccc ccccccctaa cgttactggc
       61 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
      121 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
      181 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
      241 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
      301 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
      361 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
      421 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
      481 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
      541 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac
      601 catggtcatg aaaacattta acatttctca acaagatcta gaattagtag aagtagcgac
      661 agagaagatt acaatgcttt atgaggataa taaacatcat gtgggagcgg caattcgtac
      721 gaaaacagga gaaatcattt cggcagtaca tattgaagcg tatataggac gagtaactgt
      781 ttgtgcagaa gccattgcga ttggtagtgc agtttcgaat ggacaaaagg attttgacac
      841 gattgtagct gttagacacc cttattctga cgaagtagat agaagtattc gagtggtaag
      901 tccttgtggt atgtgtaggg agttgatttc agactatgca ccagattgtt ttgtgttaat
      961 agaaatgaat ggcaagttag tcaaaactac gattgaagaa ctcattccac tcaaatatac
     1021 ccgaaattaa aagtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg
     1081 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     1141 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
     1201 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     1261 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     1321 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     1381 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     1441 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     1501 ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
     1561 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     1621 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc
     1681 atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc
     1741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2641 tatcaaaaag gatcttcacc tagatccttt tgcggccggc cgcaaatcaa tctaaagtat
     2701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     3361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3721 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     3781 tcgtcttcac tcgaggagct tggcccattg catacgttgt atccatatca taatatgtac
     3841 atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat
     3901 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3961 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     4021 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     4081 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     4141 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     4201 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgcta
     4261 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     4321 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     4381 taaaagagcc cacaacccct cactcggcgc gccagtcctc cgattgactg agtcgcccgg
     4441 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     4501 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc
     4561 tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct
     4621 ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc
     4681 tgcgtcggta ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg
     4741 agttcggaac acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt
     4801 gtggcccgac ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctta
     4861 gaggagggat atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct
     4921 gaatttttgc tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc
     4981 gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatat gggcccgggc
     5041 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc
     5101 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa
     5161 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc
     5221 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac
     5281 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac
     5341 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt
     5401 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgcccccata
     5461 tggccatatg agatcttata tggggcaccc ccgccccttg taaacttccc tgaccctgac
     5521 atgacaagag ttactaacag cccctctctc caagctcact tacaggctct ctacttagtc
     5581 cagcacgaag tctggagacc tctggcggca gcctaccaag aacaactgga ccgaccggtg
     5641 gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac
     5701 ctagaacctc gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa
     5761 gtagacggca tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt
     5821 ggaccatcct ctagactgcc ggatcccagt gtggtggtac gggaattatg gcggcggggg
     5881 gcaggatgga ggacggttcc ttggatatca cccagagtat tgaagacgac ccacttctgg
     5941 atgcccagct tctcccacac cactcattac aagctcactt tagaccccga ttccatcctc
     6001 ttcctacagt catcatagtg aatcttctgt ggtttattca tctcgtgttt gttgttttag
     6061 catttttaac aggtgtgctt tgttcttatc ctaatccaaa tgaggacaag tgcccaggaa
     6121 attacacaaa cccattgaaa gttcagacgg ttataatcct tgggaaagtt attttgtgga
     6181 ttctccattt actccttgaa tgctacatcc agtatcacca cagcaaaatc agaaaccgag
     6241 gctataactt gatctaccga tcaacaaggc atctcaagag acttgcgttg atgatacagt
     6301 cctctggcaa cacagtgctt ctcctcatac tgtgcatgca gcactccttc ccagagcctg
     6361 gcagattgta tcttgacctc attctggcca tcttggcact ggaactcatc tgttccctga
     6421 tatgtctcct catttacaca gtgaaaatcc ggagatttaa taaagctaaa ccagagcctg
     6481 atatacttga agaagaaaaa atctatgctt accccagcaa tattacctcg gagactggat
     6541 tcagaactat ttcaagccta gaagaaattg ttgaaaagca aggagacacc attgaatacc
     6601 tgaagcgaca caatgcgctg ctgagtaagc gattgttggc tctcacttcc tcagacctgg
     6661 gctgtcagcc aagtagaacg ggaattctgc agtcgacggt accgcgggcc cgggatccac
     6721 cggtcgccac catggtgagt gtgatcgcta aacaaatgac ctacaaggtt tatatgtcag
     6781 gcacggtcaa tggacactac tttgaggtcg aaggcgatgg aaaaggaaag ccttacgagg
     6841 gagagcagac agtaaagctc actgtcacca agggtggacc tctgccattt gcttgggata
     6901 ttttatcacc acagcttcag tacggaagca taccattcac caagtaccct gaagacatcc
     6961 ctgattattt caagcagtca ttccctgagg gatatacatg ggagaggagc atgaactttg
     7021 aagatggtgc agtgtgtact gtcagcaatg attccagcat ccaaggcaac tgtttcatct
     7081 acaatgtcaa aatctctggt gagaactttc ctcccaatgg acctgttatg cagaagaaga
     7141 cacagggctg ggaacccagc actgagcgtc tctttgcacg agatggaatg ctgataggaa
     7201 acgattatat ggctctgaag ttggaaggag gtggtcacta tttgtgtgaa tttaaatcta
     7261 cttacaaggc aaagaagcct gtgaggatgc cagggcgcca cgagattgac cgcaaactgg
     7321 atgtaaccag tcacaacagg gattacacat ctgttgagca gtgtgaaata gccattgcac
     7381 gccactcttt gctcggttaa
//
