LOCUS       pPB.DEST-ITGB1si        8986 bp DNA     circular SYN 25-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   James
  TITLE     Direct Submission
  JOURNAL   Exported Feb 25, 2024 from SnapGene Viewer 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pPB.DEST-ITGB1(WT)-mRuby2
FEATURES             Location/Qualifiers
     source          1..8986
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    670..983
                     /label=piggyBac 5' terminal repeat
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1393..1668
                     /label=chicken beta-actin promoter
     misc_feature    1670..2627
                     /label=chicken beta actin intron
     misc_feature    2788..2823
                     /label=MCS 1
                     /note="multiple cloning site, part 1"
     CDS             2831..5224
                     /codon_start=1
                     /label=Integrin Beta1
                     /translation="MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNC
                     GWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAE
                     KLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVK
                     SLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNV
                     LSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAG
                     FHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEE
                     FQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKS
                     YCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVIL
                     QYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCR
                     KENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKC
                     RVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQT
                     CLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEK
                     DVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKL
                     LMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK"
     misc_feature    5061..5081
                     /label=shRNA TRCN0000029645
                     /note="https://www.ncbi.nlm.nih.gov/pubmed/21491421 "
     CDS             5240..5950
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     protein_bind    complement(5971..5995)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6034..6258
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6305..6321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6329..6345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6353..6359,6360..6377,6378..6383))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6398..6419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6510..6751
                     /label=piggyBac 3' terminal repeat
     promoter        complement(6798..6816)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6837..6853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6861..6877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6885..6891,6892..6909,6910..6915))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6930..6951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7239..7827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7998..8789,8790..8858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8859..8963)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     1441 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     1501 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1681 ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg gctctgactg
     1741 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     1801 cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct taaagggctc
     1861 cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     1921 ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
     1981 gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     2041 gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     2101 agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct ccccgagttg
     2161 ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg gggctcgccg
     2221 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     2281 gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc gaggcgcggc
     2341 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     2401 ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     2461 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     2521 gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg gctgccttcg
     2581 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     2641 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttgt
     2701 tgtgctgtct catcattttg gcaaagaatt cagatctgcg gccgcctcga gaagcttgat
     2761 caacaagttt gtacaaaaaa gcaggctggc gccggaacca attcagtcga ctggatccgg
     2821 taccaccgcc atgaatttac aaccaatttt ctggattgga ctgatcagtt cagtttgctg
     2881 tgtgtttgct caaacagatg aaaatagatg tttaaaagca aatgccaaat catgtggaga
     2941 atgtatacaa gcagggccaa attgtgggtg gtgcacaaat tcaacatttt tacaggaagg
     3001 aatgcctact tctgcacgat gtgatgattt agaagcctta aaaaagaagg gttgccctcc
     3061 agatgacata gaaaatccca gaggctccaa agatataaag aaaaataaaa atgtaaccaa
     3121 ccgtagcaaa ggaacagcag agaagctcaa gccagaggat attactcaga tccaaccaca
     3181 gcagttggtt ttgcgattaa gatcagggga gccacagaca tttacattaa aattcaagag
     3241 agctgaagac tatcccattg acctctacta ccttatggac ctgtcttact caatgaaaga
     3301 cgatttggag aatgtaaaaa gtcttggaac agatctgatg aatgaaatga ggaggattac
     3361 ttcggacttc agaattggat ttggctcatt tgtggaaaag actgtgatgc cttacattag
     3421 cacaacacca gctaagctca ggaacccttg cacaagtgaa cagaactgca ccagcccatt
     3481 tagctacaaa aatgtgctca gtcttactaa taaaggagaa gtatttaatg aacttgttgg
     3541 aaaacagcgc atatctggaa atttggattc tccagaaggt ggtttcgatg ccatcatgca
     3601 agttgcagtt tgtggatcac tgattggctg gaggaatgtt acacggctgc tggtgttttc
     3661 cacagatgcc gggtttcact ttgctggaga tgggaaactt ggtggcattg ttttaccaaa
     3721 tgatggacaa tgtcacctgg aaaataatat gtacacaatg agccattatt atgattatcc
     3781 ttctattgct caccttgtcc agaaactgag tgaaaataat attcagacaa tttttgcagt
     3841 tactgaagaa tttcagcctg tttacaagga gctgaaaaac ttgatcccta agtcagcagt
     3901 aggaacatta tctgcaaatt ctagcaatgt aattcagttg atcattgatg catacaattc
     3961 cctttcctca gaagtcattt tggaaaacgg caaattgtca gaaggagtaa caataagtta
     4021 caaatcttac tgcaagaacg gggtgaatgg aacaggggaa aatggaagaa aatgttccaa
     4081 tatttccatt ggagatgagg ttcaatttga aattagcata acttcaaata agtgtccaaa
     4141 aaaggattct gacagcttta aaattaggcc tctgggcttt acggaggaag tagaggttat
     4201 tcttcagtac atctgtgaat gtgaatgcca aagcgaaggc atccctgaaa gtcccaagtg
     4261 tcatgaagga aatgggacat ttgagtgtgg cgcgtgcagg tgcaatgaag ggcgtgttgg
     4321 tagacattgt gaatgcagca cagatgaagt taacagtgaa gacatggatg cttactgcag
     4381 gaaagaaaac agttcagaaa tctgcagtaa caatggagag tgcgtctgcg gacagtgtgt
     4441 ttgtaggaag agggataata caaatgaaat ttattctggc aaattctgcg agtgtgataa
     4501 tttcaactgt gatagatcca atggcttaat ttgtggagga aatggtgttt gcaagtgtcg
     4561 tgtgtgtgag tgcaacccca actacactgg cagtgcatgt gactgttctt tggatactag
     4621 tacttgtgaa gccagcaacg gacagatctg caatggccgg ggcatctgcg agtgtggtgt
     4681 ctgtaagtgt acagatccga agtttcaagg gcaaacgtgt gagatgtgtc agacctgcct
     4741 tggtgtctgt gctgagcata aagaatgtgt tcagtgcaga gccttcaata aaggagaaaa
     4801 gaaagacaca tgcacacagg aatgttccta ttttaacatt accaaggtag aaagtcggga
     4861 caaattaccc cagccggtcc aacctgatcc tgtgtcccat tgtaaggaga aggatgttga
     4921 cgactgttgg ttctatttta cgtattcagt gaatgggaac aacgaggtca tggttcatgt
     4981 tgtggagaat ccagagtgtc ccactggtcc agacatcatt ccaattgtag ctggtgtggt
     5041 tgctggaatt gttcttattg gccttgcgtt actcctcatt tggaagcttt taatgataat
     5101 tcatgacaga agggagtttg ctaaatttga aaaggagaaa atgaatgcca aatgggacac
     5161 gggtgaaaat cctatttata agagtgccgt aacaactgtg gtcaatccga agtatgaggg
     5221 aaaaggcggc ggcggccgca tggtgtctaa gggcgaagag ctgatcaagg aaaatatgcg
     5281 tatgaaggtg gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg
     5341 agaaggcaat ccgtacatgg gaactcaaac catgaggatc aaagtcatcg agggaggacc
     5401 cctgccattt gcctttgaca ttcttgccac gtcgttcatg tatggcagcc gtacttttat
     5461 caagtacccg aaaggcattc ctgatttctt taaacagtcc tttcctgagg gttttacttg
     5521 ggaaagagtt acgagatacg aagatggtgg agtcgtcacc gtcatgcagg acaccagcct
     5581 tgaggatggc tgtctcgttt accacgtcca agtcagaggg gtaaactttc cctccaatgg
     5641 tcccgtgatg cagaagaaga ccaagggttg ggagcctaat acagagatga tgtatccagc
     5701 agatggtggt ctgaggggat acactcatat ggcactgaaa gttgatggtg gtggccatct
     5761 gtcttgctct ttcgtaacaa cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc
     5821 cggtatccat gccgttgatc accgcctgga aaggttagag gaaagtgaca atgaaatgtt
     5881 cgtagtacaa cgcgaacacg cagttgccaa gttcgccggg cttggtggtg ggatggacga
     5941 gctgtacaag tagtaactcg agatatctag acccagcttt cttgtacaaa gtggttgatc
     6001 atcgatgatc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6061 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6121 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6181 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6241 atgcggtggg ctctatggct tctgaggcgg aaagaacctg cagcccaagc ttggcgtaat
     6301 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6361 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6421 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccatt
     6481 catgaatgaa ttcatgtcga catactagtt aaaagttttg ttactttata gaagaaattt
     6541 tgagtttttg ttttttttta ataaataaat aaacataaat aaattgtttg ttgaatttat
     6601 tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta ataaataaac
     6661 ctcgatatac agaccgataa aacacatgcg tcaattttac gcatgattat ctttaacgta
     6721 cgtcacaata tgattatctt tctagggtta atctagtata cgcgtatgcg gccgcttaat
     6781 taatccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     6841 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6901 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6961 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7021 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     7681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7921 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7981 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8041 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8101 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8161 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8221 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     8281 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8341 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8401 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8461 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8521 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8581 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8641 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8701 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8761 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8821 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8881 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8941 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
