LOCUS       pENTR223-ABL2(K2        6237 bp DNA     circular SYN 13-MAR-2024
DEFINITION  Donor vector for inserting an attB-flanked gene to generate an entry
            vector in the Gateway(R) system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A, Li 
            N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley 
            JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S, 
            Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala JS, 
            Hill DE, Vidal M.
  TITLE     Human ORFeome version 1.1: a platform for reverse proteomics.
  JOURNAL   Genome Res. 2004;14:2128-35.
  PUBMED    15489335
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
  JOURNAL   Exported Mar 13, 2024 from SnapGene Viewer 7.1.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2004"; volume: "14"; pages: "2128-35"
FEATURES             Location/Qualifiers
     source          1..6237
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    570..669
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             672..4109
                     /codon_start=1
                     /product="Human tyrosine kinase arg gene mRNA
                     https://www.ncbi.nlm.nih.gov/nucleotide/M35296.1?report=gen
                     bank&log$=nuclalign&blast_rank=7&RID=0Z5N43W1015"
                     /label=ABL2(K281M)
                     /translation="MVLGTVLLPPNSYGRDQDTSLCCLCTEASESALPDLTEALHRPYG
                     CDVEPQALNEAIRWSSKENLLGATESDPNLFVALYDFVASGDNTLSITKGEKLRVLGYN
                     QNGEWSEVRSKNGQGWVPSNYITPVNSLEKHSWYHGPVSRSAAEYLLSSLINGSFLVRE
                     SESSPGQLSISLRYEGRVYHYRINTTADGKVYVTAESRFSTLAELVHHHSTVADGLVTT
                     LHYPAPKCNKPTVYGVSPIHDKWEMERTDITMKHKLGGGQYGEVYVGVWKKYSLTVAVM
                     TLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTLEPPFYIVTEYMPYGNLLDYLREC
                     NREEVTAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHVVKVADFGLSRLMT
                     GDTYTAHAGAKFPIKWTAPESLAYNTFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQV
                     YDLLEKGYRMEQPEGCPPKVYELMRACWKWSPADRPSFAETHQAFETMFHDSSISEEVA
                     EELGRAASSSSVVPYLPRLPILPSKTRTLKKQVENKENIEGAQDATENSASSLAPGFIR
                     GAQASSGSPALPRKQRDKSPSSLLEDAKETCFTRDRKGGFFSSFMKKRNAPTPPKRSSS
                     FREMENQPHKKYELTGNFSSVASLQHADGFSFTPAQQEANLVPPKCYGGSFAQRNLCND
                     DGGGGGGSGTAGGGWSGITGFFTPRLIKKTLGLRAGKPTASDDTSKPFPRSNSTSSMSS
                     GLPEQDRMAMTLPRNCQRSKLQLERTVSTSSQPEENVDRANDMLPKKSEESAAPSRERP
                     KAKLLPRGATALPLRTPSGDLAITEKDPPGVGVAGVAAAPKGKEKNGGARLGMAGVPED
                     GEQPGWPSPAKAAPVLPTTHNHKVPVLISPTLKHTPADVQLIGTDSQGNKFKLLSEHQV
                     TSSGDKDRPRRVKPKCAPPPPPVMRLLQHPSICSDPTEEPTALTAGQSTSETQEGGKKA
                     ALGAVPISGKAGRPVMPPPQVPLPTSSISPAKMANGTAGTKVALRKTKQAAEKISADKI
                     SKEALLECADLLSSALTEPVPNSQLVDTGHQLLDYCSGYVDCIPQTRNKFAFREAVSKL
                     ELSLQELQVSSAAAGVPGTNPVLNNLLSCVQEISDVVQR"
     misc_difference 1512..1514
                     /label=K281M
     protein_bind    complement(4115..4214)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4233..4251)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4256..4272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4703..5494
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5587..6175
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccccc aaataatgat tttattttga ctgatagtga
      601 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      661 aagttggcac catggtcctt gggacagttc tccttccacc taatagttat ggcagagatc
      721 aggacacttc actttgctgc ctgtgcactg aggcctcaga atctgctcta cccgacttaa
      781 cagaagcttt gcatcgtccc tatggttgtg atgttgaacc ccaggcacta aatgaggcta
      841 tcaggtggag ctccaaggag aacttgctcg gagccactga gagtgaccct aatctcttcg
      901 ttgcacttta tgattttgta gcaagtggtg ataacacact cagcatcact aaaggtgaaa
      961 agctacgagt ccttggttac aaccagaatg gtgagtggag tgaagttcgc tctaagaatg
     1021 ggcagggctg ggtgccaagc aactacatca ccccagtgaa cagcctggaa aaacactcct
     1081 ggtaccatgg acctgtgtca cgcagtgcag ctgagtatct gctcagcagt ctaatcaatg
     1141 gcagcttcct ggtgcgagaa agtgagagta gccctgggca gctgtccatc tcgctcaggt
     1201 acgagggacg tgtgtatcac tacaggatca ataccactgc agatggcaag gtgtatgtga
     1261 ctgctgagag ccgcttcagc accttggcag agcttgtaca ccatcactcc acagtggctg
     1321 atgggctggt gacaacatta cactacccag cacccaagtg taataagcct acagtctatg
     1381 gtgtgtcccc catccacgac aaatgggaaa tggagcgaac agatattacc atgaagcaca
     1441 aacttggggg cggtcagtat ggagaggttt acgttggcgt ctggaagaaa tacagcctta
     1501 cagttgctgt gatgacattg aaggaagata ccatggaggt agaagaattc ctgaaagaag
     1561 ctgcagtaat gaaggaaatc aagcatccta atctggtaca acttttaggt gtgtgtactt
     1621 tggagccacc attttacatt gtgactgaat acatgccata cgggaatttg ctggattacc
     1681 tccgagaatg caaccgagaa gaggtgactg cagttgtgct gctctacatg gccactcaga
     1741 tttcttctgc aatggagtac ttagagaaga agaatttcat ccatagagat cttgcagctc
     1801 gtaactgcct agtgggagaa aaccatgtgg taaaagtggc tgactttggc ttaagtagat
     1861 tgatgactgg agacacttat actgctcatg ctggagccaa atttcctatt aagtggacag
     1921 caccagagag tcttgcctac aataccttct caattaaatc tgacgtctgg gcttttgggg
     1981 tattgttgtg ggaaattgct acctatggaa tgtcaccata tccaggtatt gacctgtctc
     2041 aggtctatga cctactagaa aaaggatatc gaatggaaca gcctgaggga tgccccccta
     2101 aggtttatga acttatgaga gcatgctgga agtggagccc tgccgatagg ccctcttttg
     2161 ctgaaacaca ccaagctttt gaaaccatgt tccatgactc cagcatttct gaagaggtag
     2221 ctgaggagct tgggagagcc gcctcctcgt catctgttgt tccatacctg ccccggctac
     2281 ctatacttcc ttccaagact cggacactga agaaacaggt ggagaacaag gagaacattg
     2341 aaggggcaca agatgccaca gaaaattctg cttccagttt agcaccaggg ttcatcagag
     2401 gtgcacaggc ctctagtgga tccccagcac tgcctcgaaa gcaaagagac aagtcaccca
     2461 gcagcctctt ggaagatgcc aaagagacat gcttcaccag ggataggaag gggggcttct
     2521 tcagctcctt catgaagaag agaaatgctc ctacaccccc caaacgcagc agctccttcc
     2581 gagaaatgga gaatcagccc cataagaaat acgaactcac gggtaacttc tcatctgttg
     2641 cttctctaca gcatgctgat gggttctctt tcactcctgc ccagcaagag gcgaatctgg
     2701 tgccacccaa gtgctatggg gggagctttg cacagaggaa cctctgtaat gacgacggtg
     2761 gtgggggtgg gggcagtggc actgctgggg gtgggtggtc tggcatcaca ggcttcttta
     2821 caccacgctt aatcaaaaag acactgggct tacgagcagg taaacccaca gccagtgatg
     2881 acacttccaa gccttttcca aggtcaaact ctacatcttc catgtcctca gggcttccag
     2941 agcaggatag gatggcaatg acccttccca ggaactgcca gaggtccaaa ctccagctgg
     3001 aaaggacagt gtccacctct tctcagccag aagagaatgt ggacagggcc aatgacatgc
     3061 ttccaaaaaa atcagaggaa agtgctgctc caagcaggga gagaccaaaa gccaagttat
     3121 tgcccagagg agccacagct cttcctctca gaacaccctc tggggatcta gccattacag
     3181 agaaggaccc tccaggggtg ggagtggctg gagtggcagc tgcccccaag ggtaaagaga
     3241 agaatggtgg ggcacgactt gggatggctg gagttccaga ggatggagag cagccgggct
     3301 ggccttctcc agccaaggct gcccccgtcc tcccaaccac tcacaaccac aaagtgccag
     3361 tccttatctc acccactctg aaacacactc cagctgacgt gcagctcatt ggcacagact
     3421 ctcaggggaa taaattcaag ctcttatctg agcatcaggt cacatcctct ggagacaagg
     3481 accgaccccg acgggtaaaa ccaaagtgtg ccccaccccc accaccagtg atgagactac
     3541 tgcagcatcc gtccatctgc tcagacccta cagaagagcc aactgcccta actgcaggac
     3601 agtccacatc agaaacacag gaaggaggaa agaaggcagc tctgggcgca gtgcccatca
     3661 gtgggaaagc tgggaggcca gtgatgcctc cacctcaagt gcctctgccc acatcttcca
     3721 tctcgccagc caaaatggcc aatggcacag caggtactaa agtggctctg agaaaaacca
     3781 aacaggccgc tgagaaaatc tcagcagaca aaatcagcaa agaggccctg ctggaatgtg
     3841 ctgacctact gtccagtgca ctcacggaac ctgtgcccaa cagccagctg gtagacactg
     3901 gacaccagct gcttgactac tgctcaggct atgtggactg catccctcaa actcgcaaca
     3961 aatttgcctt ccgagaggct gtgagcaaac tggaactcag cctgcaggag ctacaggttt
     4021 cttcagcagc tgctggtgtg cccgggacaa accctgtcct taataactta ttgtcatgtg
     4081 tacaggaaat cagtgatgtg gtgcagaggt agacacccag ctttcttgta caaagttggc
     4141 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     4201 aaaatcatta tttggccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     4261 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     4321 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     4381 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     4441 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     4501 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     4561 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     4621 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     4681 ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact
     4741 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     4801 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     4861 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     4921 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     4981 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     5041 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     5101 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     5161 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     5221 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     5281 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     5341 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     5401 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     5461 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     5521 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     5581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     5641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     5701 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     5761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     5821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     5881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     5941 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     6001 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
