LOCUS       Exported               10262 bp ds-DNA    circular SYN 29-9月-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDB5225-pUra4NotI-P41nmt1-Pil1-mCherry-natMX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   pzq
  TITLE     Direct Submission
  JOURNAL   Exported 2024年1月23日 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..676
                     /label="downstream#2 of ura4(S.pombe) 3'UTR"
                     /note="downstream#2 of ura4(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     misc_feature    678..685
                     /note="Linearization Site"
                     /note="color: #993300"
     misc_feature    687..1498
                     /label="upstream of ura4(S.pombe) 5'UTR"
                     /note="upstream of ura4(S.pombe) 5'UTR"
                     /note="color: #f8d3a9"
     5'UTR           1499..1649
                     /label="ura4(S.pombe) 5'UTR"
                     /note="ura4(S.pombe) 5'UTR"
                     /note="color: #b4abac"
     misc_feature    1650..2444
                     /label="ura4(S.pombe) CDS from pDB4712"
                     /note="ura4(S.pombe) CDS from pDB4712"
                     /note="color: #f58a5e"
     3'UTR           2445..2636
                     /label="ura4(S.pombe) 3'UTR"
                     /note="ura4(S.pombe) 3'UTR"
                     /note="color: #b4abac"
     misc_feature    2637..2879
                     /label="downstream#1 of ura4(S.pombe) 3'UTR"
                     /note="downstream#1 of ura4(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     rep_origin      complement(2963..3391)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 ori"
                     /note="color: #ffef86"
     primer_bind     3532..3548
                     /label="M13 fwd"
                     /note="M13 fwd"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        3558..3576
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /note="color: #c6c9d1"
     misc_feature    3585..3591
                     /label="MCS"
                     /note="MCS"
                     /note="color: #c6c9d1"
     misc_feature    3597..4761
                     /note="P41nmt1-promoter"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4680..4698
                     /note="LD214"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4752..4761
                     /note="nmt41 promoter"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             4766..4767
                     /codon_start=1
                     /note="open reading frame#1"
                     /note="color: #a6acb3"
                     /translation=""
     CDS             4766..4767
                     /codon_start=3
                     /note="mcherry"
                     /note="color: #ff0000"
                     /translation=""
     misc_feature    4766..4767
                     /note="pCR4-TOPO"
                     /note="color: #a6acb3"
     misc_feature    4766..4767
                     /note="Untitled"
                     /note="color: #a6acb3"
     misc_feature    4766..4767
                     /note="LD217"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4768..5820
                     /note="Pil1-ORF"
                     /note="color: #99cc00; direction: RIGHT"
     misc_feature    5826..5848
                     /note="LD217"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    5826..5846
                     /note="oligo2314"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             5826..5836
                     /codon_start=3
                     /note="mcherry"
                     /note="color: #ff0000"
                     /translation="MVS"
     misc_feature    5826..5836
                     /note="pCR4-TOPO"
                     /note="color: #a6acb3"
     misc_feature    5826
                     /note="Untitled"
                     /note="color: #a6acb3"
     CDS             5827..6534
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent 
                     protein"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    5849..6512
                     /note="dTomato PCR"
                     /note="color: #a6acb3"
     misc_feature    complement(6513..6534)
                     /note="LD218"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(6520..6534)
                     /note="oligo2315"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    6535..6540
                     /note="1626]"
                     /note="color: #a6acb3"
     misc_feature    complement(6535..6540)
                     /note="LD218"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(6535..6539)
                     /note="oligo2315"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    6544..6778
                     /note="ADH1 terminator"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      6562..6749
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
                     /note="color: #ffffff"
     gene            6818..7940
                     /label="natMX"
                     /note="natMX"
                     /note="color: #f58a5e; direction: RIGHT"
     promoter        6818..7161
                     /label="TEF promoter"
                     /note="TEF promoter"
                     /note="color: #c6c9d1"
     CDS             7162..7737
                     /label="NrsR"
                     /note="NrsR"
                     /note="color: #b7e6d7; direction: RIGHT"
     terminator      7743..7940
                     /label="TEF terminator"
                     /note="TEF terminator"
                     /note="color: #c6c9d1"
     promoter        complement(8002..8020)
                     /label="T3 promoter"
                     /note="T3 promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(8041..8057)
                     /label="M13 rev"
                     /note="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    complement(8065..8081)
                     /label="lac operator"
                     /note="lac operator"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(8089..8119)
                     /label="lac promoter"
                     /note="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    complement(8443..9031)
                     /label="ori"
                     /note="ori"
                     /note="color: #ffef86; direction: LEFT"
     CDS             complement(9202..9993)
                     /label="AmpR"
                     /note="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
     CDS             complement(9994..10062)
                     /label="AmpR(Signal Sequence)"
                     /note="AmpR(Signal Sequence)"
                     /note="color: #b7e6d7; direction: LEFT"
     promoter        complement(10063..10167)
                     /label="AmpR promoter"
                     /note="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcagcg gccgctgctt acataatgag tgagtgtcat tattgaggaa
      721 tgccatgtca gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt
      781 tttgcccaag gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc
      841 actcttttta gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg
      901 cgtattggat atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat
      961 gagcaaactg cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat
     1021 cgcatggaag atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa
     1081 attttgattc catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg
     1141 ctatatgtat gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc
     1201 tttctctaaa taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt
     1261 atttgtttgt atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc
     1321 atggtgaaaa agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag
     1381 ccttaatgcc cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt
     1441 gaatatattt tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt
     1501 ttgtctttat aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat
     1561 ctctgcacat gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg
     1621 atcgaaatta aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag
     1681 ctagagctga ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa
     1741 agcaaagcaa cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg
     1801 tagataaaat tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt
     1861 tcgaccagga tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct
     1921 ttgaggatcg caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg
     1981 tgtacaaaat tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta
     2041 ttatacaagg cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg
     2101 aaatgtcttc caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt
     2161 ttgagaagca taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc
     2221 aaagcgacta cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg
     2281 gacagcaata tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg
     2341 ttggtcgtgg agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag
     2401 aagctggttg gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa
     2461 aatttttttg gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg
     2521 ttttatgtca gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta
     2581 gcgactaaaa tattaactat tatagataaa caccttggga ataaaaagta atttgctata
     2641 gtaatttatt aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg
     2701 aataagaaaa gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa
     2761 agatgtatgt agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa
     2821 acattggtgt tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt
     2881 gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     2941 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3001 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3061 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3121 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3181 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3241 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3301 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3361 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     3421 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3481 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     3541 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggcccgatc
     3601 agaaaattat cgccataaaa gacagaataa gtcatcagcg gttgtttcat ttcctatatt
     3661 ttttttttat ttttttattt tttaataagg gaaaatttaa cgtctaagga tacagaagat
     3721 tgttagcaca ttaaagtaat aaaggcttaa gtagtaagtg ccttagcatg ttattgtatt
     3781 tcaaaggaca taatctaaaa taataacaat atcatttctc acaagttatt caattttctt
     3841 ttttttttct aataatatca agaatgtatt atttgtttga cataagtcaa ctaatttatt
     3901 taatatgctg gattaatctt gcagacatgt aaattaacaa gttttagtca aataacgttg
     3961 aagtttcaat gaactcaaat aatttctctt tttttttata taactctaat ctgatttata
     4021 ttttccgcag ggatcaactg aagttatgac atttggattg gatcacttat aaccttggtc
     4081 gccaaataat acaaaaatca gcgttataaa acaaagaagg tttttgttaa gaaattaatc
     4141 ctctttcttg ataagaaagt tgaaccgaaa ttgcagatac tgatatatga aaataatacc
     4201 cacaattttg ggaatagcgc aagcctcaat ttaaacaata ggtgaggaca catgataatg
     4261 acctcaatga ttgttagaag aaaagagcct cattacaaaa tcgaaaaatg aatggttggg
     4321 tacaagtttc caaaacatgg taaagtggac tttgcgtatg agacgtaaat agaaaaaaac
     4381 acttgttata tgttttctag aattattgtt gtctctttat ggttggatga tgcaaaatag
     4441 taatttcggt tagttgctgt aaaacaccac gagacaaata gatatggata tttattaaat
     4501 caggaaaaac gtaactctcg gctactggat ggttcagtca cccaacgatt actggggaga
     4561 gaaaacaggg caaaagcaaa gcttaaagga atccgattgt cattcggcaa tgtgcagcga
     4621 aactaaaaac cggataatgg acctgttaat cgaaacattg aagataaagg aagaggaatc
     4681 ctggcatatc atcaattgaa taagttgaat taattatttc aatctcattc tcactttctg
     4741 acttatagtc gctttgttaa agctagcatg atgaaccgtg cgtactcttt aagaaacagc
     4801 cgtgccccta cggcctcaca attgttaaac ccccctcctc cctcctcttc cactcgtagt
     4861 ggtcgtttgt ttgccccctt gagtcatacc atgcgtatca accacgctgc caccttcacc
     4921 cctgacttgg ctaagcgttt ggctgtcttg gtcaagatgg aaaagaacgt gatgcgcagt
     4981 atggaggtca ccatccgtgg ccgtcgtgat tgtgctcgcc aactttccta ctggggtgag
     5041 gactgcgatg acgatatcag cgacgtcacc gacaaattgg gtgtcttgtt ttacgagatg
     5101 gccgagttgg agaactactt gatcgatcgt tatgaccaat accgtatgac gttgaagtcc
     5161 atccgtaaca tcgagtcctc tgtccaaccc tctcgtgaga agaagcaaaa gctcttggat
     5221 caaatctatg ctcttaagca taaggatcct gaatcccctc gcttggtcac catggagcaa
     5281 gagcttgttc gtgaggaggc tgcctgtttg gttgccgagg ctcaactttc caacaccacc
     5341 cgtgaaaagt tcaagcaagc catgaccttc aacttggatg ccctccacga gcatgccgag
     5401 aagctcaacc tcattgccac ctatggcaga catttgttga acttgattga cgacaccccc
     5461 gtcactcccg gtgaggctcg tcctgcctac gatggttatg agacttcccg ccaaatcgtc
     5521 atggatgctg agcacgcctt gagctcttgg gttcccagcc aacccgccgt caactttgtc
     5581 aagcctcaaa tcgaggatga tgcccaaagt gacgctcgtt cctggaacga gtatgaggct
     5641 caaggtgagc ccgtccccgt tgagcaagtc acccacttac aagacgacgt tcaatccgac
     5701 actagcgaga tcattgagaa cgagcctggc atgcaccctc atgtccatgc tgagcgtatg
     5761 tctcgcattg cttctgagtc tagcgatgcc gttcctcagc aaaccgccgt ccaggttgct
     5821 gctagcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     5881 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     5941 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     6001 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     6061 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     6121 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     6181 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     6241 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     6301 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     6361 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     6421 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     6481 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagagatct
     6541 taacgcgcca cttctaaata agcgaatttc ttatgattta tgatttttat tattaaataa
     6601 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
     6661 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagtatgag
     6721 gtcgctctta ttgaccacac ctctaccggc agatccgcta gggataacag ggtaatatgt
     6781 ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct
     6841 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
     6901 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     6961 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     7021 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     7081 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     7141 cacatccgaa cataaacaac catgggtacc actcttgacg acacggctta ccggtaccgc
     7201 accagtgtcc cgggggacgc cgaggccatc gaggcactgg atgggtcctt caccaccgac
     7261 accgtcttcc gcgtcaccgc caccggggac ggcttcaccc tgcgggaggt gccggtggac
     7321 ccgcccctga ccaaggtgtt ccccgacgac gaatcggacg acgaatcgga cgacggggag
     7381 gacggcgacc cggactcccg gacgttcgtc gcgtacgggg acgacggcga cctggcgggc
     7441 ttcgtggtca tctcgtactc ggcgtggaac cgccggctga ccgtcgagga catcgaggtc
     7501 gccccggagc accgggggca cggggtcggg cgcgcgttga tggggctcgc gacggagttc
     7561 gccggcgagc ggggcgccgg gcacctctgg ctggaggtca ccaacgtcaa cgcaccggcg
     7621 atccacgcgt accggcggat ggggttcacc ctctgcggcc tggacaccgc cctgtacgac
     7681 ggcaccgcct cggacggcga gcggcaggcg ctctacatga gcatgccctg cccctaatca
     7741 gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata
     7801 ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca
     7861 tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt
     7921 gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca cctgcaggta
     7981 atcacgtgcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     8041 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acataggagc
     8101 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca cattaattgc
     8161 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8221 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcaaagc
     8821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8881 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     9121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9241 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9601 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    10141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    10201 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    10261 tc
//
