LOCUS       Exported                9209 bp ds-DNA    circular SYN 11-11月-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDB4875-pUra4NotI-P41nmt1-mECtrine-natMX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   pzq
  TITLE     Direct Submission
  JOURNAL   Exported 2024年1月23日 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..676
                     /label="downstream#2 of ura4(S.pombe) 3'UTR"
                     /note="downstream#2 of ura4(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     misc_feature    678..685
                     /note="Linearization Site"
                     /note="color: #993300"
     misc_feature    687..1498
                     /label="upstream of ura4(S.pombe) 5'UTR"
                     /note="upstream of ura4(S.pombe) 5'UTR"
                     /note="color: #f8d3a9"
     5'UTR           1499..1649
                     /label="ura4(S.pombe) 5'UTR"
                     /note="ura4(S.pombe) 5'UTR"
                     /note="color: #b4abac"
     misc_feature    1650..2444
                     /label="ura4(S.pombe) CDS from pDB4712"
                     /note="ura4(S.pombe) CDS from pDB4712"
                     /note="color: #f58a5e"
     3'UTR           2445..2636
                     /label="ura4(S.pombe) 3'UTR"
                     /note="ura4(S.pombe) 3'UTR"
                     /note="color: #b4abac"
     misc_feature    2637..2879
                     /label="downstream#1 of ura4(S.pombe) 3'UTR"
                     /note="downstream#1 of ura4(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     rep_origin      complement(2963..3391)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 ori"
                     /note="color: #ffef86"
     primer_bind     3532..3548
                     /label="M13 fwd"
                     /note="M13 fwd"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        3558..3576
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /note="color: #c6c9d1"
     misc_feature    3585..3591
                     /label="MCS"
                     /note="MCS"
                     /note="color: #c6c9d1"
     misc_feature    3597..4761
                     /note="P41nmt1-promoter"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4680..4698
                     /note="LD214"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             4768..5481
                     /codon_start=1
                     /note="Citrine"
                     /note="color: #00ff00"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    5482..5487
                     /note="1626]"
                     /note="color: #a6acb3"
     misc_feature    complement(5482..5487)
                     /note="LD218"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(5482..5486)
                     /note="oligo2315"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    5491..5725
                     /note="ADH1 terminator"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      5509..5696
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
                     /note="color: #ffffff"
     gene            5765..6887
                     /label="natMX"
                     /note="natMX"
                     /note="color: #f58a5e; direction: RIGHT"
     promoter        5765..6108
                     /label="TEF promoter"
                     /note="TEF promoter"
                     /note="color: #c6c9d1"
     CDS             6109..6684
                     /label="NrsR"
                     /note="NrsR"
                     /note="color: #b7e6d7; direction: RIGHT"
     terminator      6690..6887
                     /label="TEF terminator"
                     /note="TEF terminator"
                     /note="color: #c6c9d1"
     promoter        complement(6949..6967)
                     /label="T3 promoter"
                     /note="T3 promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(6988..7004)
                     /label="M13 rev"
                     /note="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    complement(7012..7028)
                     /label="lac operator"
                     /note="lac operator"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(7036..7066)
                     /label="lac promoter"
                     /note="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    complement(7390..7978)
                     /label="ori"
                     /note="ori"
                     /note="color: #ffef86; direction: LEFT"
     CDS             complement(8149..8940)
                     /label="AmpR"
                     /note="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
     CDS             complement(8941..9009)
                     /label="AmpR(Signal Sequence)"
                     /note="AmpR(Signal Sequence)"
                     /note="color: #b7e6d7; direction: LEFT"
     promoter        complement(9010..9114)
                     /label="AmpR promoter"
                     /note="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcagcg gccgctgctt acataatgag tgagtgtcat tattgaggaa
      721 tgccatgtca gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt
      781 tttgcccaag gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc
      841 actcttttta gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg
      901 cgtattggat atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat
      961 gagcaaactg cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat
     1021 cgcatggaag atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa
     1081 attttgattc catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg
     1141 ctatatgtat gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc
     1201 tttctctaaa taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt
     1261 atttgtttgt atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc
     1321 atggtgaaaa agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag
     1381 ccttaatgcc cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt
     1441 gaatatattt tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt
     1501 ttgtctttat aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat
     1561 ctctgcacat gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg
     1621 atcgaaatta aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag
     1681 ctagagctga ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa
     1741 agcaaagcaa cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg
     1801 tagataaaat tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt
     1861 tcgaccagga tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct
     1921 ttgaggatcg caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg
     1981 tgtacaaaat tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta
     2041 ttatacaagg cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg
     2101 aaatgtcttc caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt
     2161 ttgagaagca taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc
     2221 aaagcgacta cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg
     2281 gacagcaata tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg
     2341 ttggtcgtgg agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag
     2401 aagctggttg gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa
     2461 aatttttttg gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg
     2521 ttttatgtca gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta
     2581 gcgactaaaa tattaactat tatagataaa caccttggga ataaaaagta atttgctata
     2641 gtaatttatt aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg
     2701 aataagaaaa gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa
     2761 agatgtatgt agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa
     2821 acattggtgt tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt
     2881 gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     2941 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3001 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3061 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3121 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3181 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3241 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3301 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3361 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     3421 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3481 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     3541 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggcccgatc
     3601 agaaaattat cgccataaaa gacagaataa gtcatcagcg gttgtttcat ttcctatatt
     3661 ttttttttat ttttttattt tttaataagg gaaaatttaa cgtctaagga tacagaagat
     3721 tgttagcaca ttaaagtaat aaaggcttaa gtagtaagtg ccttagcatg ttattgtatt
     3781 tcaaaggaca taatctaaaa taataacaat atcatttctc acaagttatt caattttctt
     3841 ttttttttct aataatatca agaatgtatt atttgtttga cataagtcaa ctaatttatt
     3901 taatatgctg gattaatctt gcagacatgt aaattaacaa gttttagtca aataacgttg
     3961 aagtttcaat gaactcaaat aatttctctt tttttttata taactctaat ctgatttata
     4021 ttttccgcag ggatcaactg aagttatgac atttggattg gatcacttat aaccttggtc
     4081 gccaaataat acaaaaatca gcgttataaa acaaagaagg tttttgttaa gaaattaatc
     4141 ctctttcttg ataagaaagt tgaaccgaaa ttgcagatac tgatatatga aaataatacc
     4201 cacaattttg ggaatagcgc aagcctcaat ttaaacaata ggtgaggaca catgataatg
     4261 acctcaatga ttgttagaag aaaagagcct cattacaaaa tcgaaaaatg aatggttggg
     4321 tacaagtttc caaaacatgg taaagtggac tttgcgtatg agacgtaaat agaaaaaaac
     4381 acttgttata tgttttctag aattattgtt gtctctttat ggttggatga tgcaaaatag
     4441 taatttcggt tagttgctgt aaaacaccac gagacaaata gatatggata tttattaaat
     4501 caggaaaaac gtaactctcg gctactggat ggttcagtca cccaacgatt actggggaga
     4561 gaaaacaggg caaaagcaaa gcttaaagga atccgattgt cattcggcaa tgtgcagcga
     4621 aactaaaaac cggataatgg acctgttaat cgaaacattg aagataaagg aagaggaatc
     4681 ctggcatatc atcaattgaa taagttgaat taattatttc aatctcattc tcactttctg
     4741 acttatagtc gctttgttaa agctagcatg agtaaaggag aagaactttt cactggagtt
     4801 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     4861 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     4921 aaactacctg ttccatggcc aacacttgtc actactttgg gttatggtct aatgtgcttt
     4981 gctagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
     5041 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     5101 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     5161 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     5221 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     5281 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     5341 cctgtccttt taccagacaa ccattacctg tcctatcaat ctaagctttc gaaagatccc
     5401 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     5461 ggcatggatg aactatacaa aagatcttaa cgcgccactt ctaaataagc gaatttctta
     5521 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     5581 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
     5641 aggttgcttt ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga
     5701 tccgctaggg ataacagggt aatatgttta gcttgcctcg tccccgccgg gtcacccggc
     5761 cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc
     5821 atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc
     5881 catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg
     5941 cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta
     6001 gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa
     6061 aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat gggtaccact
     6121 cttgacgaca cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag
     6181 gcactggatg ggtccttcac caccgacacc gtcttccgcg tcaccgccac cggggacggc
     6241 ttcaccctgc gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa
     6301 tcggacgacg aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg
     6361 tacggggacg acggcgacct ggcgggcttc gtggtcatct cgtactcggc gtggaaccgc
     6421 cggctgaccg tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc
     6481 gcgttgatgg ggctcgcgac ggagttcgcc ggcgagcggg gcgccgggca cctctggctg
     6541 gaggtcacca acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc
     6601 tgcggcctgg acaccgccct gtacgacggc accgcctcgg acggcgagcg gcaggcgctc
     6661 tacatgagca tgccctgccc ctaatcagta ctgacaataa aaagattctt gttttcaaga
     6721 acttgtcatt tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg
     6781 tgatttatat tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga
     6841 aagtaatatc atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat
     6901 actaacgccg ccatccacct gcaggtaatc acgtgccagc ttttgttccc tttagtgagg
     6961 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7021 gctcacaatt ccacacaaca taggagccgg aagcataaag tgtaaagcct ggggtgccta
     7081 atgagtgagg taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7141 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7201 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7261 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7321 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7381 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7441 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7501 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7561 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7621 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7681 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7741 agccactggt aacaggatta gcaaagcgag gtatgtaggc ggtgctacag agttcttgaa
     7801 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7861 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7921 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9121 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9181 aaataggcgt atcacgaggc cctttcgtc
//
