LOCUS       Exported                8774 bp ds-DNA    circular SYN 11-11月-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDB4884-pHis5NotI-P41nmt1-mECtrine-kanMX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   pzq
  TITLE     Direct Submission
  JOURNAL   Exported 2024年1月23日 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..667
                     /label="downstream#2 of his5(S.pombe) 3'UTR)"
                     /note="downstream#2 of his5(S.pombe) 3'UTR)"
                     /note="color: #f8d3a9"
     misc_feature    669..676
                     /note="Linearization Site"
                     /note="color: #993300"
     misc_feature    678..1106
                     /label="upstream of his5(S.pombe) 5'UTR"
                     /note="upstream of his5(S.pombe) 5'UTR"
                     /note="color: #f8d3a9"
     5'UTR           1107..1187
                     /label="his5(S.pombe) 5'UTR"
                     /note="his5(S.pombe) 5'UTR"
                     /note="color: #c6c9d1"
     CDS             1188..1838
                     /label="his5(S.pombe) CDS"
                     /note="his5(S.pombe) CDS"
                     /note="color: #d59687; direction: RIGHT"
     3'UTR           1839..2153
                     /label="his5(S.pombe) 3'UTR"
                     /note="his5(S.pombe) 3'UTR"
                     /note="color: #c6c9d1"
     misc_feature    2154..2216
                     /label="downstream#1 of his5(S.pombe) 3'UTR"
                     /note="downstream#1 of his5(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     rep_origin      complement(2299..2727)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 ori"
                     /note="color: #ffef86"
     primer_bind     2868..2884
                     /label="M13 fwd"
                     /note="M13 fwd"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        2894..2912
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /note="color: #c6c9d1"
     misc_feature    2933..4097
                     /note="P41nmt1-promoter"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4016..4034
                     /note="LD214"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             4104..4817
                     /codon_start=1
                     /note="Citrine"
                     /note="color: #00ff00"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    4818..4823
                     /note="1626]"
                     /note="color: #a6acb3"
     misc_feature    complement(4818..4823)
                     /note="LD218"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(4818..4822)
                     /note="oligo2315"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4827..5061
                     /note="ADH1 terminator"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      4845..5032
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
                     /note="color: #ffffff"
     gene            5101..6457
                     /label="kanMX"
                     /note="kanMX"
                     /note="color: #f58a5e; direction: RIGHT"
     promoter        5101..5444
                     /label="TEF promoter"
                     /note="TEF promoter"
                     /note="color: #c6c9d1"
     CDS             5445..6254
                     /label="KanR"
                     /note="KanR"
                     /note="color: #b7e6d7; direction: RIGHT"
     terminator      6260..6457
                     /label="TEF terminator"
                     /note="TEF terminator"
                     /note="color: #c6c9d1"
     misc_feature    6496..6501
                     /note="SacI site"
                     /note="color: #993366"
     promoter        complement(6514..6532)
                     /label="T3 promoter"
                     /note="T3 promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(6553..6569)
                     /label="M13 rev"
                     /note="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    complement(6577..6593)
                     /label="lac operator"
                     /note="lac operator"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(6601..6631)
                     /label="lac promoter"
                     /note="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     protein_bind    complement(6646..6667)
                     /label="CAP binding site"
                     /note="CAP binding site"
                     /note="color: #31849b; direction: LEFT"
     rep_origin      complement(6955..7543)
                     /direction=LEFT
                     /label="ori"
                     /note="ori"
                     /note="color: #ffef86"
     CDS             complement(7714..8505)
                     /label="AmpR"
                     /note="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
     CDS             complement(8506..8574)
                     /label="AmpR(Signal Sequence)"
                     /note="AmpR(Signal Sequence)"
                     /note="color: #b7e6d7; direction: LEFT"
     promoter        complement(8575..8679)
                     /label="AmpR promoter"
                     /note="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggatggaacg attgaatact gtgctactct ttatgtaata taacgaattc
      241 aactgttcaa cgtatgtgac tgctctctgc ttctcggtat atatttactt gaaggaatta
      301 atttttcttt gccaatggtc tcttttacca attgcagtct ggcttaaagc aatgaggttg
      361 taattaaatg gcttgagacc tagaacaaac accttttaca atccttgttt actggttttg
      421 atagtaaatt cggttcgctt gtggtgtaca ttatattttt taggctagtc gtgatttttt
      481 gataatatca atattcttag atttgagaca ttcatgcttt ttaatttgat caatcgtttg
      541 tatcttcatt aattagtagg tggtttgata gaacatttag ttgcttgttt gactacaaat
      601 atctgcagaa tggattcggt taataaaaaa aatgtttgag gaattaattt tcgctttgga
      661 gtggaggagc ggccgctcct caattcgatg ttgcagattt tacaagtttt taaaatgtat
      721 ttcattatta ctttttatat gcctaataaa aaagccatag tttaatctat agataacttt
      781 ttttccagtg cactaacgga cgttacattc ccatacaaaa ctgcgtagtt aaagctaagg
      841 aaaagttaat atcatgttaa ttaaatacgc tatttacaat aagacattga actcatttat
      901 atcgttgaat atgaataacc aatttcagcg aatttttaac aaacatcgtt cacctcgttt
      961 aaggatatct tgtgtatggg gtgttgactt gctttatcga ataattaccg tacctgtaat
     1021 tggcttgctg gatatagcgg tagtctaata tctagcaaaa atcttttggg tgaaaaggct
     1081 tgcaatttca cgacaccgaa ctatttgtca ttttttaata aggaagtttt ccataaattc
     1141 ctgtaattct cggttgatct aattgaaaag agtagttttg catcacgatg aggagggctt
     1201 ttgtagaaag aaatacgaac gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc
     1261 ccttacctga agagtcgaat tttattgatg aacttataac ttccaagcat gcaaaccaaa
     1321 agggagaaca agtaatccaa gtagacacgg gaattggatt cttggatcac atgtatcatg
     1381 cactggctaa acatgcaggc tggagcttac gactttactc aagaggtgat ttaatcatcg
     1441 atgatcatca cactgcagaa gatactgcta ttgcacttgg tattgcattc aagcaggcta
     1501 tgggtaactt tgccggcgtt aaaagatttg gacatgctta ttgtccactt gacgaagctc
     1561 tttctagaag cgtagttgac ttgtcgggac ggccctatgc tgttatcgat ttgggattaa
     1621 agcgtgaaaa ggttggggaa ttgtcctgtg aaatgatccc tcacttacta tattcctttt
     1681 cggtagcagc tggaattact ttgcatgtta cctgcttata tggtagtaat gaccatcatc
     1741 gtgctgaaag cgcttttaaa tctctggctg ttgccatgcg cgcggctact agtcttactg
     1801 gaagttctga agtcccaagc acgaagggag tgttgtaaag atgaattgga ttatgtcagg
     1861 aaaagaacga caattttgca tccaaattgt ctaaatttta gagttgcttg aaaacaatag
     1921 aaccttactt gctttataat tacgttaatt agaagcgtta tctcgtgaag gaatatagta
     1981 cgtagccgta taaattgaat tgaatgttca gcttatagaa tagagacact ttgctgttca
     2041 atgcgtcgtc acttaccata ctcactttat tatacgactt taagtataaa ctccgcggtt
     2101 atggtaaaat taatgatgca caaacgtccg attccatatg ggtacactac aattaaatac
     2161 ttttaagtaa cgctgatttc ttatttttta tccttggtgt atttattgtt tataccgcta
     2221 gggcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     2281 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2341 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2401 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2461 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2521 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2581 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2641 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2701 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     2761 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     2821 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     2881 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc ccgatcagaa
     2941 aattatcgcc ataaaagaca gaataagtca tcagcggttg tttcatttcc tatatttttt
     3001 ttttattttt ttatttttta ataagggaaa atttaacgtc taaggataca gaagattgtt
     3061 agcacattaa agtaataaag gcttaagtag taagtgcctt agcatgttat tgtatttcaa
     3121 aggacataat ctaaaataat aacaatatca tttctcacaa gttattcaat tttctttttt
     3181 ttttctaata atatcaagaa tgtattattt gtttgacata agtcaactaa tttatttaat
     3241 atgctggatt aatcttgcag acatgtaaat taacaagttt tagtcaaata acgttgaagt
     3301 ttcaatgaac tcaaataatt tctctttttt tttatataac tctaatctga tttatatttt
     3361 ccgcagggat caactgaagt tatgacattt ggattggatc acttataacc ttggtcgcca
     3421 aataatacaa aaatcagcgt tataaaacaa agaaggtttt tgttaagaaa ttaatcctct
     3481 ttcttgataa gaaagttgaa ccgaaattgc agatactgat atatgaaaat aatacccaca
     3541 attttgggaa tagcgcaagc ctcaatttaa acaataggtg aggacacatg ataatgacct
     3601 caatgattgt tagaagaaaa gagcctcatt acaaaatcga aaaatgaatg gttgggtaca
     3661 agtttccaaa acatggtaaa gtggactttg cgtatgagac gtaaatagaa aaaaacactt
     3721 gttatatgtt ttctagaatt attgttgtct ctttatggtt ggatgatgca aaatagtaat
     3781 ttcggttagt tgctgtaaaa caccacgaga caaatagata tggatattta ttaaatcagg
     3841 aaaaacgtaa ctctcggcta ctggatggtt cagtcaccca acgattactg gggagagaaa
     3901 acagggcaaa agcaaagctt aaaggaatcc gattgtcatt cggcaatgtg cagcgaaact
     3961 aaaaaccgga taatggacct gttaatcgaa acattgaaga taaaggaaga ggaatcctgg
     4021 catatcatca attgaataag ttgaattaat tatttcaatc tcattctcac tttctgactt
     4081 atagtcgctt tgttaaagct agcatgagta aaggagaaga acttttcact ggagttgtcc
     4141 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
     4201 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
     4261 tacctgttcc atggccaaca cttgtcacta ctttgggtta tggtctaatg tgctttgcta
     4321 gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc gaaggttatg
     4381 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
     4441 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
     4501 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
     4561 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     4621 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     4681 tccttttacc agacaaccat tacctgtcct atcaatctaa gctttcgaaa gatcccaacg
     4741 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     4801 tggatgaact atacaaaaga tcttaacgcg ccacttctaa ataagcgaat ttcttatgat
     4861 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
     4921 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
     4981 tgctttctca ggtatagtat gaggtcgctc ttattgacca cacctctacc ggcagatccg
     5041 ctagggataa cagggtaata tgtttagctt gcctcgtccc cgccgggtca cccggccagc
     5101 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
     5161 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
     5221 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
     5281 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
     5341 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
     5401 ttgctaggat acagttctca catcacatcc gaacataaac aaccatgggt aaggaaaaga
     5461 ctcacgtttc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     5521 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
     5581 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     5641 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     5701 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggcaaa acagcattcc
     5761 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     5821 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     5881 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     5941 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat
     6001 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     6061 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     6121 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     6181 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     6241 atgagttttt ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
     6301 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     6361 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     6421 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg
     6481 ccatccagtt taaacgagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     6541 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6601 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     6661 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     6721 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     6781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     6841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     6901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7381 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     7621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     7681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     7741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     8641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     8701 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     8761 gaggcccttt cgtc
//
