LOCUS       Exported               11864 bp ds-DNA    circular SYN 01-12月-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDB5253-pUra4NotI-P41nmt1-mECitrine-CtNbr1-naMX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   pzq
  TITLE     Direct Submission
  JOURNAL   Exported 2024年1月23日 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..676
                     /label="downstream#2 of ura4(S.pombe) 3'UTR"
                     /note="downstream#2 of ura4(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     misc_feature    678..685
                     /note="Linearization Site"
                     /note="color: #993300"
     misc_feature    687..1498
                     /label="upstream of ura4(S.pombe) 5'UTR"
                     /note="upstream of ura4(S.pombe) 5'UTR"
                     /note="color: #f8d3a9"
     5'UTR           1499..1649
                     /label="ura4(S.pombe) 5'UTR"
                     /note="ura4(S.pombe) 5'UTR"
                     /note="color: #b4abac"
     misc_feature    1650..2444
                     /label="ura4(S.pombe) CDS from pDB4712"
                     /note="ura4(S.pombe) CDS from pDB4712"
                     /note="color: #f58a5e"
     3'UTR           2445..2636
                     /label="ura4(S.pombe) 3'UTR"
                     /note="ura4(S.pombe) 3'UTR"
                     /note="color: #b4abac"
     misc_feature    2637..2879
                     /label="downstream#1 of ura4(S.pombe) 3'UTR"
                     /note="downstream#1 of ura4(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     rep_origin      complement(2963..3391)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 ori"
                     /note="color: #ffef86"
     primer_bind     3532..3548
                     /label="M13 fwd"
                     /note="M13 fwd"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        3558..3576
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /note="color: #c6c9d1"
     misc_feature    3585..3591
                     /label="MCS"
                     /note="MCS"
                     /note="color: #c6c9d1"
     misc_feature    3597..4761
                     /note="P41nmt1-promoter"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4680..4698
                     /note="LD214"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             4768..5481
                     /codon_start=1
                     /note="Citrine"
                     /note="color: #00ff00"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    complement(5482..5486)
                     /note="oligo2315"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(5482..5486)
                     /note="LD218"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    5482..5486
                     /note="1626]"
                     /note="color: #a6acb3"
     CDS             5488..8136
                     /codon_start=1
                     /note="CtNbr1"
                     /note="color: #993366"
                     /translation="MATPAVQVTPDTLITFKVSLDGSPRRFKLPLRDVGINVLEGKLRA
                     ALNIPPEVDAVFERFSDSAAAFVVLDQNKPAVYKQLYRAAKAKQKLKLRVTTKKPEEAN
                     NKPQPAAVEDAPEQEAQQPVESVPAPEREADSATAENIASAPERPASPMPEEKQVEITQ
                     AAAEQSTTETNSAIESFGEKLKTSTEYLREVLNEASTAPAAAPEQLPPVPAPAPAAVPL
                     PCRVDCPPLRSHYAVCCNNCDRTIPDAHYHCSTCDDGDFDLCQECVDRGVTCKGADHWM
                     IKRFVRDGVIINSTTERLPPKTKPAPTETPKPATTSASPPRTFPTGPAAPVTSVTPPIA
                     PPVPTMTSERIIPVFNDFLYSSMRTCNCCVRDFPEVAFVHCTTCEDYDLCRSCFAKNRH
                     GHHPGHAFVPAVEGTRLEPEVARLLAPGRGVIHNALCDGCDNTIRGVRHKCLNCPDWDY
                     CSQCILNAPVIHPGHRFVPIYEPLEQADSNANFRFQSRPVHFGICCDGPLCNNGPNSNR
                     YIVGDRYKCAVCHDTDFCEACEASPANTHNKTHPLIKFKTPVRHVSVTTTGENEHGRQM
                     PPMGDRRVPRPRAPESAPHQCPAPSNVGNVQTVVDVKPIEPEVPVKEEKVEEKVEKKPE
                     PVVEKEPSAEELEATFVRDTVQDGTVLAPNHLFEQTWVLRNTGKVAWPAGCSVKFVGGD
                     YMGRVDSAHPAASKEVEESCESTVCDRAVQPGEEAPFTVLLRTPYRACRVISHWRLTTP
                     KGTKFGHRLWCDVVVEKPKSRSPSPAPAFVEEKAKEVSPEPEPATSQMIFPKLDRESPV
                     MSVHEEEARSEGVPTSVAVPSIQDEFEDCEDVDWPENGSEDGFLTDEEYDVLDASDEEF
                     SMGLHPSTSKQK"
     misc_feature    5488..5613
                     /note="exon1"
                     /note="color: #a6acb3"
     misc_feature    5614..8136
                     /note="exon2"
                     /note="color: #a6acb3"
     CDS             6151..6153
                     /codon_start=1
                     /note="222"
                     /note="color: #993366"
                     /translation="L"
     CDS             7063..7065
                     /codon_start=1
                     /note="526"
                     /note="color: #993366"
                     /translation="C"
     misc_feature    8138..8142
                     /note="1626]"
                     /note="color: #a6acb3"
     misc_feature    complement(8138..8142)
                     /note="LD218"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(8138..8141)
                     /note="oligo2315"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    8146..8380
                     /note="ADH1 terminator"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      8164..8351
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
                     /note="color: #ffffff"
     gene            8420..9542
                     /label="natMX"
                     /note="natMX"
                     /note="color: #f58a5e; direction: RIGHT"
     promoter        8420..8763
                     /label="TEF promoter"
                     /note="TEF promoter"
                     /note="color: #c6c9d1"
     CDS             8764..9339
                     /label="NrsR"
                     /note="NrsR"
                     /note="color: #b7e6d7; direction: RIGHT"
     terminator      9345..9542
                     /label="TEF terminator"
                     /note="TEF terminator"
                     /note="color: #c6c9d1"
     promoter        complement(9604..9622)
                     /label="T3 promoter"
                     /note="T3 promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(9643..9659)
                     /label="M13 rev"
                     /note="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    complement(9667..9683)
                     /label="lac operator"
                     /note="lac operator"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(9691..9721)
                     /label="lac promoter"
                     /note="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    complement(10045..10633)
                     /label="ori"
                     /note="ori"
                     /note="color: #ffef86; direction: LEFT"
     CDS             complement(10804..11595)
                     /label="AmpR"
                     /note="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
     CDS             complement(11596..11664)
                     /label="AmpR(Signal Sequence)"
                     /note="AmpR(Signal Sequence)"
                     /note="color: #b7e6d7; direction: LEFT"
     promoter        complement(11665..11769)
                     /label="AmpR promoter"
                     /note="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcagcg gccgctgctt acataatgag tgagtgtcat tattgaggaa
      721 tgccatgtca gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt
      781 tttgcccaag gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc
      841 actcttttta gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg
      901 cgtattggat atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat
      961 gagcaaactg cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat
     1021 cgcatggaag atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa
     1081 attttgattc catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg
     1141 ctatatgtat gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc
     1201 tttctctaaa taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt
     1261 atttgtttgt atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc
     1321 atggtgaaaa agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag
     1381 ccttaatgcc cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt
     1441 gaatatattt tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt
     1501 ttgtctttat aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat
     1561 ctctgcacat gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg
     1621 atcgaaatta aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag
     1681 ctagagctga ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa
     1741 agcaaagcaa cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg
     1801 tagataaaat tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt
     1861 tcgaccagga tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct
     1921 ttgaggatcg caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg
     1981 tgtacaaaat tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta
     2041 ttatacaagg cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg
     2101 aaatgtcttc caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt
     2161 ttgagaagca taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc
     2221 aaagcgacta cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg
     2281 gacagcaata tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg
     2341 ttggtcgtgg agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag
     2401 aagctggttg gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa
     2461 aatttttttg gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg
     2521 ttttatgtca gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta
     2581 gcgactaaaa tattaactat tatagataaa caccttggga ataaaaagta atttgctata
     2641 gtaatttatt aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg
     2701 aataagaaaa gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa
     2761 agatgtatgt agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa
     2821 acattggtgt tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt
     2881 gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     2941 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3001 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3061 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3121 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3181 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3241 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3301 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3361 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     3421 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3481 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     3541 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggcccgatc
     3601 agaaaattat cgccataaaa gacagaataa gtcatcagcg gttgtttcat ttcctatatt
     3661 ttttttttat ttttttattt tttaataagg gaaaatttaa cgtctaagga tacagaagat
     3721 tgttagcaca ttaaagtaat aaaggcttaa gtagtaagtg ccttagcatg ttattgtatt
     3781 tcaaaggaca taatctaaaa taataacaat atcatttctc acaagttatt caattttctt
     3841 ttttttttct aataatatca agaatgtatt atttgtttga cataagtcaa ctaatttatt
     3901 taatatgctg gattaatctt gcagacatgt aaattaacaa gttttagtca aataacgttg
     3961 aagtttcaat gaactcaaat aatttctctt tttttttata taactctaat ctgatttata
     4021 ttttccgcag ggatcaactg aagttatgac atttggattg gatcacttat aaccttggtc
     4081 gccaaataat acaaaaatca gcgttataaa acaaagaagg tttttgttaa gaaattaatc
     4141 ctctttcttg ataagaaagt tgaaccgaaa ttgcagatac tgatatatga aaataatacc
     4201 cacaattttg ggaatagcgc aagcctcaat ttaaacaata ggtgaggaca catgataatg
     4261 acctcaatga ttgttagaag aaaagagcct cattacaaaa tcgaaaaatg aatggttggg
     4321 tacaagtttc caaaacatgg taaagtggac tttgcgtatg agacgtaaat agaaaaaaac
     4381 acttgttata tgttttctag aattattgtt gtctctttat ggttggatga tgcaaaatag
     4441 taatttcggt tagttgctgt aaaacaccac gagacaaata gatatggata tttattaaat
     4501 caggaaaaac gtaactctcg gctactggat ggttcagtca cccaacgatt actggggaga
     4561 gaaaacaggg caaaagcaaa gcttaaagga atccgattgt cattcggcaa tgtgcagcga
     4621 aactaaaaac cggataatgg acctgttaat cgaaacattg aagataaagg aagaggaatc
     4681 ctggcatatc atcaattgaa taagttgaat taattatttc aatctcattc tcactttctg
     4741 acttatagtc gctttgttaa agctagcatg agtaaaggag aagaactttt cactggagtt
     4801 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     4861 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     4921 aaactacctg ttccatggcc aacacttgtc actactttgg gttatggtct aatgtgcttt
     4981 gctagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
     5041 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     5101 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     5161 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     5221 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     5281 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     5341 cctgtccttt taccagacaa ccattacctg tcctatcaat ctaagctttc gaaagatccc
     5401 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     5461 ggcatggatg aactatacaa aagatctatg gccactccag cggtgcaagt cactcccgat
     5521 acccttatca ccttcaaggt gagccttgat ggctcgcctc gtcgctttaa gctgcctctg
     5581 cgcgatgtcg gcatcaacgt cctcgagggc aagcttcgtg ctgccctgaa catccctccg
     5641 gaggtcgatg ccgtctttga gcgcttctcc gactctgccg ctgcctttgt cgtgctggac
     5701 caaaacaagc ctgccgtcta caagcaactc taccgtgctg ccaaggcgaa gcagaagctt
     5761 aagttgcgtg tcacaactaa gaagcccgag gaggctaaca acaagcctca gccggctgcc
     5821 gttgaggatg ctcccgagca ggaggctcag cagcctgtcg agtctgtccc tgcaccggag
     5881 cgcgaggctg actcggcaac cgccgagaac atcgcttctg cccctgagag acctgccagt
     5941 cccatgcctg aggagaagca ggtcgagatt acccaggctg ccgccgagca gagcaccacc
     6001 gagaccaaca gtgctattga gtcgttcggt gagaagctga agaccagcac agagtacctg
     6061 agagaggttc tcaacgaggc ctcgacagcg cctgctgctg ctccagagca gctgccacct
     6121 gttcctgcac cggctcccgc ggctgttcct cttccctgcc gcgttgattg cccgcctctg
     6181 cgttctcact acgctgtgtg ctgcaacaac tgcgaccgga ccattcccga tgcccactac
     6241 cactgctcga cctgtgacga tggcgacttt gacctgtgcc aggaatgtgt cgaccgcggg
     6301 gtcacctgca agggtgctga tcactggatg atcaagcgct ttgtccgcga tggcgtcatt
     6361 atcaacagca caacggagcg tcttccgccc aagacgaagc ctgctcccac ggaaaccccc
     6421 aagcctgcga cgacgtcggc gtcacctccg aggaccttcc ctaccggtcc tgcagctccc
     6481 gtcacttctg tcacccctcc tattgctcct ccggtgccga caatgacctc cgagcgtatt
     6541 atccccgttt tcaacgactt cctgtactca agcatgcgca cttgcaactg ctgtgtacgg
     6601 gacttcccgg aggtggcctt tgtccactgc actacttgcg aggactatga cctctgccgt
     6661 tcttgcttcg ccaagaaccg ccacggtcat caccccggcc atgcctttgt tcccgccgtt
     6721 gaaggcacca ggctggagcc cgaggtggct cgtctcctgg cccctggccg gggagtcatc
     6781 cacaatgccc tgtgcgatgg ctgcgataac accatccgtg gtgttcgtca caagtgcctg
     6841 aactgccctg actgggacta ctgctctcag tgtattctga atgcccctgt catccatccg
     6901 ggccaccgct tcgtgcccat ctacgagcct ctggagcagg cagacagtaa tgccaacttc
     6961 cgcttccagt ctcgtcctgt acacttcggc atttgctgcg atggcccact ttgcaataac
     7021 gggcccaaca gcaaccgata cattgttggc gaccgctaca agtgcgctgt ttgccacgac
     7081 accgacttct gcgaggcttg cgaggccagc cccgctaaca ctcataacaa gactcaccca
     7141 cttatcaagt tcaagactcc tgttcgccat gtgagcgtca ccaccaccgg tgagaacgag
     7201 catggtcgtc agatgccacc tatgggtgat cgcagggtgc cccgtcctcg tgctcccgag
     7261 tcagctcctc atcagtgccc tgctcccagc aacgttggca acgtccagac cgttgttgac
     7321 gtcaagccga ttgagccaga ggttcctgtc aaggaggaga aagttgagga gaaggtcgag
     7381 aagaagccag agccggttgt tgagaaggag ccatcggctg aagagctcga ggccactttc
     7441 gtccgcgaca ctgttcagga tggcaccgtc cttgctccca accacctctt cgagcagact
     7501 tgggttctgc gcaacacggg caaggtcgcc tggccggctg gctgctcggt caagttcgtt
     7561 ggtggcgact acatgggccg cgttgactcg gcccaccctg ctgcctcgaa ggaggttgag
     7621 gagtcttgcg agtcgaccgt ttgcgaccgc gccgtccagc ccggcgagga ggctcccttc
     7681 accgtgctcc tgcgcactcc ctaccgcgca tgccgtgtta tttcccactg gcgtctaacc
     7741 accccgaagg gtaccaagtt cggccaccgc ctgtggtgcg atgttgttgt tgagaagccc
     7801 aagtctcgtt ctccttctcc tgctcccgcc ttcgtcgagg agaaggccaa ggaagtgagc
     7861 cccgagcccg agccggccac cagccagatg atcttcccca agctggacag agagtcgccc
     7921 gttatgagcg ttcatgagga ggaggcccgg tcggagggtg ttcctacttc tgtcgcagtt
     7981 ccaagcatcc aggatgagtt tgaggactgc gaggacgtcg actggcctga aaacggctcg
     8041 gaggatgggt tccttactga tgaggagtac gacgttcttg atgcctcgga tgaggagttc
     8101 tccatggggt tgcacccgtc gacgagcaaa cagaagagat cttaacgcgc cacttctaaa
     8161 taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
     8221 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac
     8281 tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct tattgaccac
     8341 acctctaccg gcagatccgc tagggataac agggtaatat gtttagcttg cctcgtcccc
     8401 gccgggtcac ccggccagcg acatggaggc ccagaatacc ctccttgaca gtcttgacgt
     8461 gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac atccccatgt
     8521 ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa attacggctc
     8581 ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga attgtcccca
     8641 cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt tcttctttca
     8701 tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg aacataaaca
     8761 accatgggta ccactcttga cgacacggct taccggtacc gcaccagtgt cccgggggac
     8821 gccgaggcca tcgaggcact ggatgggtcc ttcaccaccg acaccgtctt ccgcgtcacc
     8881 gccaccgggg acggcttcac cctgcgggag gtgccggtgg acccgcccct gaccaaggtg
     8941 ttccccgacg acgaatcgga cgacgaatcg gacgacgggg aggacggcga cccggactcc
     9001 cggacgttcg tcgcgtacgg ggacgacggc gacctggcgg gcttcgtggt catctcgtac
     9061 tcggcgtgga accgccggct gaccgtcgag gacatcgagg tcgccccgga gcaccggggg
     9121 cacggggtcg ggcgcgcgtt gatggggctc gcgacggagt tcgccggcga gcggggcgcc
     9181 gggcacctct ggctggaggt caccaacgtc aacgcaccgg cgatccacgc gtaccggcgg
     9241 atggggttca ccctctgcgg cctggacacc gccctgtacg acggcaccgc ctcggacggc
     9301 gagcggcagg cgctctacat gagcatgccc tgcccctaat cagtactgac aataaaaaga
     9361 ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt
     9421 aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga
     9481 agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac
     9541 tgctgtcgat tcgatactaa cgccgccatc cacctgcagg taatcacgtg ccagcttttg
     9601 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     9661 gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca taaagtgtaa
     9721 agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct cactgcccgc
     9781 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     9841 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9901 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9961 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10021 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10081 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10381 atcgccactg gcagcagcca ctggtaacag gattagcaaa gcgaggtatg taggcggtgc
    10441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10501 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10561 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10621 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10681 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10741 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10801 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10861 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10921 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10981 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    11041 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    11101 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11161 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11221 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11281 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11341 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11401 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11461 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    11521 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    11581 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    11641 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    11701 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    11761 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    11821 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
