LOCUS       Exported                9212 bp ds-DNA    circular SYN 11-11月-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDB4887-pUra4NotI-P41nmt1-mTurquoise2-natMX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   pzq
  TITLE     Direct Submission
  JOURNAL   Exported 2024年1月23日 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..676
                     /label="downstream#2 of ura4(S.pombe) 3'UTR"
                     /note="downstream#2 of ura4(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     misc_feature    678..685
                     /note="Linearization Site"
                     /note="color: #993300"
     misc_feature    687..1498
                     /label="upstream of ura4(S.pombe) 5'UTR"
                     /note="upstream of ura4(S.pombe) 5'UTR"
                     /note="color: #f8d3a9"
     5'UTR           1499..1649
                     /label="ura4(S.pombe) 5'UTR"
                     /note="ura4(S.pombe) 5'UTR"
                     /note="color: #b4abac"
     misc_feature    1650..2444
                     /label="ura4(S.pombe) CDS from pDB4712"
                     /note="ura4(S.pombe) CDS from pDB4712"
                     /note="color: #f58a5e"
     3'UTR           2445..2636
                     /label="ura4(S.pombe) 3'UTR"
                     /note="ura4(S.pombe) 3'UTR"
                     /note="color: #b4abac"
     misc_feature    2637..2879
                     /label="downstream#1 of ura4(S.pombe) 3'UTR"
                     /note="downstream#1 of ura4(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     rep_origin      complement(2963..3391)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 ori"
                     /note="color: #ffef86"
     primer_bind     3532..3548
                     /label="M13 fwd"
                     /note="M13 fwd"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        3558..3576
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /note="color: #c6c9d1"
     misc_feature    3585..3591
                     /label="MCS"
                     /note="MCS"
                     /note="color: #c6c9d1"
     misc_feature    3597..4761
                     /note="P41nmt1-promoter"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4680..4698
                     /note="LD214"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             4768..5484
                     /codon_start=1
                     /note="mTurquoise2"
                     /note="color: #00ffff"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5485..5490
                     /note="1626]"
                     /note="color: #a6acb3"
     misc_feature    complement(5485..5490)
                     /note="LD218"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(5485..5489)
                     /note="oligo2315"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    5494..5728
                     /note="ADH1 terminator"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      5512..5699
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
                     /note="color: #ffffff"
     gene            5768..6890
                     /label="natMX"
                     /note="natMX"
                     /note="color: #f58a5e; direction: RIGHT"
     promoter        5768..6111
                     /label="TEF promoter"
                     /note="TEF promoter"
                     /note="color: #c6c9d1"
     CDS             6112..6687
                     /label="NrsR"
                     /note="NrsR"
                     /note="color: #b7e6d7; direction: RIGHT"
     terminator      6693..6890
                     /label="TEF terminator"
                     /note="TEF terminator"
                     /note="color: #c6c9d1"
     promoter        complement(6952..6970)
                     /label="T3 promoter"
                     /note="T3 promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(6991..7007)
                     /label="M13 rev"
                     /note="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    complement(7015..7031)
                     /label="lac operator"
                     /note="lac operator"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(7039..7069)
                     /label="lac promoter"
                     /note="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    complement(7393..7981)
                     /label="ori"
                     /note="ori"
                     /note="color: #ffef86; direction: LEFT"
     CDS             complement(8152..8943)
                     /label="AmpR"
                     /note="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
     CDS             complement(8944..9012)
                     /label="AmpR(Signal Sequence)"
                     /note="AmpR(Signal Sequence)"
                     /note="color: #b7e6d7; direction: LEFT"
     promoter        complement(9013..9117)
                     /label="AmpR promoter"
                     /note="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggtattagtc aaaactagta tactttttct cggagtataa tacacaatat
      241 cggtgcaaat aggttttaaa attgctccaa tcacatgttc ttaagaaaaa aacgtcaaaa
      301 gaaatctaag tattcccaaa ataattcctc ctgacatgag aagaataaaa aacactaaga
      361 tgcattgtca gtatgaaaca caagccaaag ttgctaacaa taggatcaat tcttacttac
      421 tggcaatgtt acgaggaatc ttggtaggag gatgtttggc aacataacgt tcttttgaat
      481 ttgtttttac acgacgagta atgttcttct gaaactgtgt gtttgcacga cgctgagagg
      541 gagtttgaac cgtctaaaag acatagttag gatccatgat gcacagattt ctaaagataa
      601 aactacttac aggcatgttg gtttattaaa aaagaaaaac aggaatgata agagggaaag
      661 aatacgtgta cagagcagcg gccgctgctt acataatgag tgagtgtcat tattgaggaa
      721 tgccatgtca gatttgacac aactcagaac ttattaactt tttatgattg gaacttttgt
      781 tttgcccaag gaatttttaa tggaagaagt tgttttataa atgatattga atttatatcc
      841 actcttttta gcgcatttgc atctctactt taggttcttc tttgactaga ttctaggatg
      901 cgtattggat atactggtct ttgaatcatt gtcgaaaaaa aaattcatgg ttgtacccat
      961 gagcaaactg cttttgtggt tatccatgtg cttcttttgt tcagcatcta tcttcttaat
     1021 cgcatggaag atttgattta aaccaaaaga aatattgtta aactttgaca gaagctttaa
     1081 attttgattc catacattaa atggataaaa ctcctgaagc ttagctacaa atcccactgg
     1141 ctatatgtat gcatttgtgt taaaaaagtt tgtatagatt atttaatcta ctcagcattc
     1201 tttctctaaa taggaatttg ttacttaatg gagaaaaaaa tgtttcgatt tacctagtgt
     1261 atttgtttgt atactcacgt ttaatttcaa acatccattc tatcttgtgt aatttttggc
     1321 atggtgaaaa agataatcag ccttataatc tttacaaaag taagaaattc tgtaaataag
     1381 ccttaatgcc cttgctttaa attaaaatgg ttctttttca tgataatgtt tgcactttgt
     1441 gaatatattt tagatagttc tgtgaggtat aattaagatg ttttagagac ttatacaatt
     1501 ttgtctttat aaattcttaa ttgattttac catcccagtt taactatgct tcgtcggcat
     1561 ctctgcacat gtcgtgtttt cttaccgtat tgtcctacca agaacctctt ttttgcttgg
     1621 atcgaaatta aaggtttaaa agcaaagtta tggatgctag agtatttcaa agctattcag
     1681 ctagagctga ggggatgaaa aatcccattg ccaaggaatt gttggctttg atggaagaaa
     1741 agcaaagcaa cttgtcagtc gcggtcgatt tgacgaagaa atccgaaatc ttagaattgg
     1801 tagataaaat tggaccctat gtctgtgtta tcaagacaca tattgacgtt gtcgaggatt
     1861 tcgaccagga tatggtagaa aaactggtgg ccttaggtaa aaagcatcgt tttcttatct
     1921 ttgaggatcg caaattcgca gacattggaa ataccgtcaa gctacaatat gcatctggtg
     1981 tgtacaaaat tgcttcttgg gctcatatca caaattgcca tacagtgcca ggcgagggta
     2041 ttatacaagg cctcaaagaa gttggtttac ctttgggacg tggtctcttg cttttggctg
     2101 aaatgtcttc caaaggctct ttggctactg gttcctacac agagaaaacc ttagaatggt
     2161 ttgagaagca taccgatttt tgctttggct ttatagctgg tcgtcgattt cctaaccttc
     2221 aaagcgacta cataactatg tcccctggta tcggcttgga tgttaaagga gacgggctgg
     2281 gacagcaata tcgtactcct gaagaagtga ttgtaaactg cggtagcgat atcatcattg
     2341 ttggtcgtgg agtttatgga gctggtcgta atcctgttgt cgaagccaag agatatagag
     2401 aagctggttg gaaggcatat cagcaaagac tttctcagca ttaaaaaaag actaatgtaa
     2461 aatttttttg gttggttatt gaaaaagtcg atgccttgtt tgcgtttgtt ttcctaggcg
     2521 ttttatgtca gaaggcattt agaattagta tacaagtact ctttggtaaa attttatgta
     2581 gcgactaaaa tattaactat tatagataaa caccttggga ataaaaagta atttgctata
     2641 gtaatttatt aaacatgctc ctacaacatt accacaatct tttctcttgg attgacattg
     2701 aataagaaaa gagtgaattt ttttagactt gtaatgataa ctatgtacaa agccaatgaa
     2761 agatgtatgt agatgaatgt aaaataccat gtagacaaac aagataaaac ttggttataa
     2821 acattggtgt tggaacagaa taaattagat gtcaaaaagt ttcgtcaata tcacaagctt
     2881 gctagggcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     2941 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3001 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3061 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3121 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3181 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3241 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3301 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3361 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     3421 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     3481 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     3541 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggcccgatc
     3601 agaaaattat cgccataaaa gacagaataa gtcatcagcg gttgtttcat ttcctatatt
     3661 ttttttttat ttttttattt tttaataagg gaaaatttaa cgtctaagga tacagaagat
     3721 tgttagcaca ttaaagtaat aaaggcttaa gtagtaagtg ccttagcatg ttattgtatt
     3781 tcaaaggaca taatctaaaa taataacaat atcatttctc acaagttatt caattttctt
     3841 ttttttttct aataatatca agaatgtatt atttgtttga cataagtcaa ctaatttatt
     3901 taatatgctg gattaatctt gcagacatgt aaattaacaa gttttagtca aataacgttg
     3961 aagtttcaat gaactcaaat aatttctctt tttttttata taactctaat ctgatttata
     4021 ttttccgcag ggatcaactg aagttatgac atttggattg gatcacttat aaccttggtc
     4081 gccaaataat acaaaaatca gcgttataaa acaaagaagg tttttgttaa gaaattaatc
     4141 ctctttcttg ataagaaagt tgaaccgaaa ttgcagatac tgatatatga aaataatacc
     4201 cacaattttg ggaatagcgc aagcctcaat ttaaacaata ggtgaggaca catgataatg
     4261 acctcaatga ttgttagaag aaaagagcct cattacaaaa tcgaaaaatg aatggttggg
     4321 tacaagtttc caaaacatgg taaagtggac tttgcgtatg agacgtaaat agaaaaaaac
     4381 acttgttata tgttttctag aattattgtt gtctctttat ggttggatga tgcaaaatag
     4441 taatttcggt tagttgctgt aaaacaccac gagacaaata gatatggata tttattaaat
     4501 caggaaaaac gtaactctcg gctactggat ggttcagtca cccaacgatt actggggaga
     4561 gaaaacaggg caaaagcaaa gcttaaagga atccgattgt cattcggcaa tgtgcagcga
     4621 aactaaaaac cggataatgg acctgttaat cgaaacattg aagataaagg aagaggaatc
     4681 ctggcatatc atcaattgaa taagttgaat taattatttc aatctcattc tcactttctg
     4741 acttatagtc gctttgttaa agctagcatg gtttctaagg gtgaagaact ctttactggt
     4801 gtcgttccta tcttggttga acttgatgga gatgttaatg gacacaagtt ttcagtctct
     4861 ggtgaaggag aaggtgatgc tacttatggt aagcttaccc ttaaattcat ttgcactact
     4921 ggaaaacttc ctgtcccttg gcctactctt gtcactaccc tgtcttgggg tgttcaatgc
     4981 tttgctcgtt atcctgacca tatgaagcaa cacgatttct ttaagagtgc tatgcctgaa
     5041 ggttatgttc aagagcgtac tattttcttc aaagacgatg gtaactataa gactcgtgct
     5101 gaagttaaat ttgagggtga tactttagtt aaccgtattg agctcaaagg aattgatttt
     5161 aaagaggatg gtaatattct tggtcataaa ttagaataca attacttttc tgataatgtt
     5221 tacattactg ccgataaaca aaagaatggt atcaaggcta acttcaagat tcgtcataac
     5281 attgaagatg gcggagtaca acttgctgat cattaccaac aaaacactcc tattggtgac
     5341 ggtcctgttt tgttgcctga taaccattat ctttctactc aatccaaatt gtctaaagat
     5401 cctaatgaaa aacgtgatca catggtcctt ttggaatttg ttaccgctgc cggtattacc
     5461 ttgggtatgg atgaattgta caagagatct taacgcgcca cttctaaata agcgaatttc
     5521 ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt
     5581 taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag
     5641 gtcaggttgc tttctcaggt atagtatgag gtcgctctta ttgaccacac ctctaccggc
     5701 agatccgcta gggataacag ggtaatatgt ttagcttgcc tcgtccccgc cgggtcaccc
     5761 ggccagcgac atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg
     5821 ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc
     5881 atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac
     5941 ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct
     6001 gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt
     6061 taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac catgggtacc
     6121 actcttgacg acacggctta ccggtaccgc accagtgtcc cgggggacgc cgaggccatc
     6181 gaggcactgg atgggtcctt caccaccgac accgtcttcc gcgtcaccgc caccggggac
     6241 ggcttcaccc tgcgggaggt gccggtggac ccgcccctga ccaaggtgtt ccccgacgac
     6301 gaatcggacg acgaatcgga cgacggggag gacggcgacc cggactcccg gacgttcgtc
     6361 gcgtacgggg acgacggcga cctggcgggc ttcgtggtca tctcgtactc ggcgtggaac
     6421 cgccggctga ccgtcgagga catcgaggtc gccccggagc accgggggca cggggtcggg
     6481 cgcgcgttga tggggctcgc gacggagttc gccggcgagc ggggcgccgg gcacctctgg
     6541 ctggaggtca ccaacgtcaa cgcaccggcg atccacgcgt accggcggat ggggttcacc
     6601 ctctgcggcc tggacaccgc cctgtacgac ggcaccgcct cggacggcga gcggcaggcg
     6661 ctctacatga gcatgccctg cccctaatca gtactgacaa taaaaagatt cttgttttca
     6721 agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta
     6781 gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc
     6841 agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc
     6901 gatactaacg ccgccatcca cctgcaggta atcacgtgcc agcttttgtt ccctttagtg
     6961 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7021 tccgctcaca attccacaca acataggagc cggaagcata aagtgtaaag cctggggtgc
     7081 ctaatgagtg aggtaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7141 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7201 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7261 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7321 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7381 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7441 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7501 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7561 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7621 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7681 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7741 agcagccact ggtaacagga ttagcaaagc gaggtatgta ggcggtgcta cagagttctt
     7801 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7861 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7921 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7981 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8041 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8101 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8161 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8221 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8281 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8341 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8401 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8461 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8521 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8581 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8641 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8701 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8761 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8821 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8881 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8941 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9001 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9061 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9121 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9181 taaaaatagg cgtatcacga ggccctttcg tc
//
