LOCUS       Exported                9436 bp ds-DNA    circular SYN 11-11月-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDB4890-pAde6-P41nmt1-NheI-mTurquoise2-BglII-hphMX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   pzq
  TITLE     Direct Submission
  JOURNAL   Exported 2024年1月23日 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..567
                     /label="downstream#2 of ade6(S.pombe) 3'UTR"
                     /note="downstream#2 of ade6(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     misc_feature    569..576
                     /note="Linearization Site"
                     /note="color: #993300"
     misc_feature    578..800
                     /label="upstream of ade6(S.pombe) 5'UTR"
                     /note="upstream of ade6(S.pombe) 5'UTR"
                     /note="color: #f8d3a9"
     5'UTR           801..846
                     /label="ade6(S.pombe) 5'UTR"
                     /note="ade6(S.pombe) 5'UTR"
                     /note="color: #c6c9d1"
     CDS             847..2505
                     /label="ade6(S.pombe) CDS"
                     /note="ade6(S.pombe) CDS"
                     /note="color: #f6989d; direction: RIGHT"
     3'UTR           2506..2545
                     /label="ade6(S.pombe) 3'UTR"
                     /note="ade6(S.pombe) 3'UTR"
                     /note="color: #c6c9d1"
     misc_feature    2546..2651
                     /label="downstream#1 of ade6(S.pombe) 3'UTR"
                     /note="downstream#1 of ade6(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     rep_origin      complement(2734..3162)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 ori"
                     /note="color: #ffef86"
     primer_bind     3303..3319
                     /label="M13 fwd"
                     /note="M13 fwd"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        3329..3347
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /note="color: #c6c9d1"
     misc_feature    3356..3362
                     /label="MCS"
                     /note="MCS"
                     /note="color: #c6c9d1"
     misc_feature    3368..4532
                     /note="P41nmt1-promoter"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4451..4469
                     /note="LD214"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             4539..5255
                     /codon_start=1
                     /note="mTurquoise2"
                     /note="color: #00ffff"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5256..5261
                     /note="1626]"
                     /note="color: #a6acb3"
     misc_feature    complement(5256..5261)
                     /note="LD218"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(5256..5260)
                     /note="oligo2315"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    5265..5499
                     /note="ADH1 terminator"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      5283..5470
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
                     /note="color: #ffffff"
     gene            5539..7114
                     /label="hphMX6"
                     /note="hphMX6"
                     /note="color: #f58a5e; direction: RIGHT"
     promoter        5539..5882
                     /label="TEF promoter"
                     /note="TEF promoter"
                     /note="color: #c6c9d1"
     CDS             5883..6911
                     /label="HygR"
                     /note="HygR"
                     /note="color: #b7e6d7; direction: RIGHT"
     terminator      6917..7114
                     /label="TEF terminator"
                     /note="TEF terminator"
                     /note="color: #c6c9d1"
     promoter        complement(7176..7194)
                     /label="T3 promoter"
                     /note="T3 promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(7215..7231)
                     /label="M13 rev"
                     /note="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    complement(7239..7255)
                     /label="lac operator"
                     /note="lac operator"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(7263..7293)
                     /label="lac promoter"
                     /note="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     rep_origin      complement(7617..8205)
                     /direction=LEFT
                     /label="ori"
                     /note="ori"
                     /note="color: #ffef86"
     CDS             complement(8376..9167)
                     /label="AmpR"
                     /note="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
     CDS             complement(9168..9236)
                     /label="AmpR(Signal Sequence)"
                     /note="AmpR(Signal Sequence)"
                     /note="color: #b7e6d7; direction: LEFT"
     promoter        complement(9237..9341)
                     /label="AmpR promoter"
                     /note="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttagc ggccgctaaa cgttgcttta tatatggata
      601 acgacatata catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct
      661 gctttaaagt tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc
      721 aaaactcaaa taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat
      781 tcgttttttc aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca
      841 tcgaatatga gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg
      901 atggtagagg cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct
      961 cctgccaaac aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat
     1021 gcaattgttg aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac
     1081 actgatgcct tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg
     1141 cgatgcattc aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt
     1201 cctgaatttt gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt
     1261 tatccttttg tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt
     1321 catcaaccat ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt
     1381 gaaaagttcg ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga
     1441 aaagtttatg cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta
     1501 tatgcccctg ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat
     1561 gcggtgcgca cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat
     1621 ggtgagacca ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc
     1681 attgaagctt gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct
     1741 ttcagcgaaa tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt
     1801 ttaggtactg atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc
     1861 ggtgcaactt tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac
     1921 gttaccatca tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac
     1981 gtcaaagatc ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta
     2041 agcaagatga aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact
     2101 attgtttcag ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca
     2161 agagggttgc gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt
     2221 gctgcaatga cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga
     2281 gttgactctc ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct
     2341 atcaataata gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc
     2401 tcccttttgg ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag
     2461 gctgataaat tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata
     2521 actgttaaat gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga
     2581 ttgtaaaatg tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt
     2641 gctgcgacgc agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2701 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2761 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2821 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2881 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2941 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3001 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     3061 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     3121 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
     3181 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     3241 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     3301 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
     3361 cgggcccgat cagaaaatta tcgccataaa agacagaata agtcatcagc ggttgtttca
     3421 tttcctatat ttttttttta tttttttatt ttttaataag ggaaaattta acgtctaagg
     3481 atacagaaga ttgttagcac attaaagtaa taaaggctta agtagtaagt gccttagcat
     3541 gttattgtat ttcaaaggac ataatctaaa ataataacaa tatcatttct cacaagttat
     3601 tcaattttct tttttttttc taataatatc aagaatgtat tatttgtttg acataagtca
     3661 actaatttat ttaatatgct ggattaatct tgcagacatg taaattaaca agttttagtc
     3721 aaataacgtt gaagtttcaa tgaactcaaa taatttctct ttttttttat ataactctaa
     3781 tctgatttat attttccgca gggatcaact gaagttatga catttggatt ggatcactta
     3841 taaccttggt cgccaaataa tacaaaaatc agcgttataa aacaaagaag gtttttgtta
     3901 agaaattaat cctctttctt gataagaaag ttgaaccgaa attgcagata ctgatatatg
     3961 aaaataatac ccacaatttt gggaatagcg caagcctcaa tttaaacaat aggtgaggac
     4021 acatgataat gacctcaatg attgttagaa gaaaagagcc tcattacaaa atcgaaaaat
     4081 gaatggttgg gtacaagttt ccaaaacatg gtaaagtgga ctttgcgtat gagacgtaaa
     4141 tagaaaaaaa cacttgttat atgttttcta gaattattgt tgtctcttta tggttggatg
     4201 atgcaaaata gtaatttcgg ttagttgctg taaaacacca cgagacaaat agatatggat
     4261 atttattaaa tcaggaaaaa cgtaactctc ggctactgga tggttcagtc acccaacgat
     4321 tactggggag agaaaacagg gcaaaagcaa agcttaaagg aatccgattg tcattcggca
     4381 atgtgcagcg aaactaaaaa ccggataatg gacctgttaa tcgaaacatt gaagataaag
     4441 gaagaggaat cctggcatat catcaattga ataagttgaa ttaattattt caatctcatt
     4501 ctcactttct gacttatagt cgctttgtta aagctagcat ggtttctaag ggtgaagaac
     4561 tctttactgg tgtcgttcct atcttggttg aacttgatgg agatgttaat ggacacaagt
     4621 tttcagtctc tggtgaagga gaaggtgatg ctacttatgg taagcttacc cttaaattca
     4681 tttgcactac tggaaaactt cctgtccctt ggcctactct tgtcactacc ctgtcttggg
     4741 gtgttcaatg ctttgctcgt tatcctgacc atatgaagca acacgatttc tttaagagtg
     4801 ctatgcctga aggttatgtt caagagcgta ctattttctt caaagacgat ggtaactata
     4861 agactcgtgc tgaagttaaa tttgagggtg atactttagt taaccgtatt gagctcaaag
     4921 gaattgattt taaagaggat ggtaatattc ttggtcataa attagaatac aattactttt
     4981 ctgataatgt ttacattact gccgataaac aaaagaatgg tatcaaggct aacttcaaga
     5041 ttcgtcataa cattgaagat ggcggagtac aacttgctga tcattaccaa caaaacactc
     5101 ctattggtga cggtcctgtt ttgttgcctg ataaccatta tctttctact caatccaaat
     5161 tgtctaaaga tcctaatgaa aaacgtgatc acatggtcct tttggaattt gttaccgctg
     5221 ccggtattac cttgggtatg gatgaattgt acaagagatc ttaacgcgcc acttctaaat
     5281 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     5341 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     5401 ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca
     5461 cctctaccgg cagatccgct agggataaca gggtaatatg tttagcttgc ctcgtccccg
     5521 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg
     5581 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta
     5641 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc
     5701 tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac
     5761 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat
     5821 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa
     5881 ccatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     5941 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     6001 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     6061 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     6121 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     6181 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     6241 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     6301 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     6361 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     6421 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     6481 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     6541 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     6601 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     6661 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     6721 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     6781 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     6841 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     6901 caaaggaata atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt
     6961 atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt
     7021 ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg
     7081 cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca
     7141 tccacctgca ggtaatcacg tgccagcttt tgttcccttt agtgagggtt aattgcgcgc
     7201 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7261 cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa
     7321 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7381 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7441 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7501 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7561 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7621 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7681 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7741 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7801 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7861 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7921 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7981 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8041 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8221 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8281 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8341 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8401 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8461 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8521 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8581 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8641 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8701 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8761 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8821 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8881 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8941 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9001 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9061 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9121 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9181 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9241 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9301 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9361 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9421 acgaggccct ttcgtc
//
