LOCUS       Exported                8777 bp ds-DNA    circular SYN 11-11月-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pDB4896-pHis5NotI-P41nmt1-mTurquoise2-kanMX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   pzq
  TITLE     Direct Submission
  JOURNAL   Exported 2024年1月23日 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    193..667
                     /label="downstream#2 of his5(S.pombe) 3'UTR)"
                     /note="downstream#2 of his5(S.pombe) 3'UTR)"
                     /note="color: #f8d3a9"
     misc_feature    669..676
                     /note="Linearization Site"
                     /note="color: #993300"
     misc_feature    678..1106
                     /label="upstream of his5(S.pombe) 5'UTR"
                     /note="upstream of his5(S.pombe) 5'UTR"
                     /note="color: #f8d3a9"
     5'UTR           1107..1187
                     /label="his5(S.pombe) 5'UTR"
                     /note="his5(S.pombe) 5'UTR"
                     /note="color: #c6c9d1"
     CDS             1188..1838
                     /label="his5(S.pombe) CDS"
                     /note="his5(S.pombe) CDS"
                     /note="color: #d59687; direction: RIGHT"
     3'UTR           1839..2153
                     /label="his5(S.pombe) 3'UTR"
                     /note="his5(S.pombe) 3'UTR"
                     /note="color: #c6c9d1"
     misc_feature    2154..2216
                     /label="downstream#1 of his5(S.pombe) 3'UTR"
                     /note="downstream#1 of his5(S.pombe) 3'UTR"
                     /note="color: #f8d3a9"
     rep_origin      complement(2299..2727)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 ori"
                     /note="color: #ffef86"
     primer_bind     2868..2884
                     /label="M13 fwd"
                     /note="M13 fwd"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        2894..2912
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /note="color: #c6c9d1"
     misc_feature    2933..4097
                     /note="P41nmt1-promoter"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    4016..4034
                     /note="LD214"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             4104..4820
                     /codon_start=1
                     /note="mTurquoise2"
                     /note="color: #00ffff"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    4821..4826
                     /note="1626]"
                     /note="color: #a6acb3"
     misc_feature    complement(4821..4826)
                     /note="LD218"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(4821..4825)
                     /note="oligo2315"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    4830..5064
                     /note="ADH1 terminator"
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      4848..5035
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for alcohol dehydrogenase 
                     1"
                     /note="color: #ffffff"
     gene            5104..6460
                     /label="kanMX"
                     /note="kanMX"
                     /note="color: #f58a5e; direction: RIGHT"
     promoter        5104..5447
                     /label="TEF promoter"
                     /note="TEF promoter"
                     /note="color: #c6c9d1"
     CDS             5448..6257
                     /label="KanR"
                     /note="KanR"
                     /note="color: #b7e6d7; direction: RIGHT"
     terminator      6263..6460
                     /label="TEF terminator"
                     /note="TEF terminator"
                     /note="color: #c6c9d1"
     misc_feature    6499..6504
                     /note="SacI site"
                     /note="color: #993366"
     promoter        complement(6517..6535)
                     /label="T3 promoter"
                     /note="T3 promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     primer_bind     complement(6556..6572)
                     /label="M13 rev"
                     /note="M13 rev"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    complement(6580..6596)
                     /label="lac operator"
                     /note="lac operator"
                     /note="color: #31849b; direction: LEFT"
     promoter        complement(6604..6634)
                     /label="lac promoter"
                     /note="lac promoter"
                     /note="color: #c6c9d1; direction: LEFT"
     protein_bind    complement(6649..6670)
                     /label="CAP binding site"
                     /note="CAP binding site"
                     /note="color: #31849b; direction: LEFT"
     rep_origin      complement(6958..7546)
                     /direction=LEFT
                     /label="ori"
                     /note="ori"
                     /note="color: #ffef86"
     CDS             complement(7717..8508)
                     /label="AmpR"
                     /note="AmpR"
                     /note="color: #b7e6d7; direction: LEFT"
     CDS             complement(8509..8577)
                     /label="AmpR(Signal Sequence)"
                     /note="AmpR(Signal Sequence)"
                     /note="color: #b7e6d7; direction: LEFT"
     promoter        complement(8578..8682)
                     /label="AmpR promoter"
                     /note="AmpR promoter"
                     /note="color: #c6c9d1; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggatggaacg attgaatact gtgctactct ttatgtaata taacgaattc
      241 aactgttcaa cgtatgtgac tgctctctgc ttctcggtat atatttactt gaaggaatta
      301 atttttcttt gccaatggtc tcttttacca attgcagtct ggcttaaagc aatgaggttg
      361 taattaaatg gcttgagacc tagaacaaac accttttaca atccttgttt actggttttg
      421 atagtaaatt cggttcgctt gtggtgtaca ttatattttt taggctagtc gtgatttttt
      481 gataatatca atattcttag atttgagaca ttcatgcttt ttaatttgat caatcgtttg
      541 tatcttcatt aattagtagg tggtttgata gaacatttag ttgcttgttt gactacaaat
      601 atctgcagaa tggattcggt taataaaaaa aatgtttgag gaattaattt tcgctttgga
      661 gtggaggagc ggccgctcct caattcgatg ttgcagattt tacaagtttt taaaatgtat
      721 ttcattatta ctttttatat gcctaataaa aaagccatag tttaatctat agataacttt
      781 ttttccagtg cactaacgga cgttacattc ccatacaaaa ctgcgtagtt aaagctaagg
      841 aaaagttaat atcatgttaa ttaaatacgc tatttacaat aagacattga actcatttat
      901 atcgttgaat atgaataacc aatttcagcg aatttttaac aaacatcgtt cacctcgttt
      961 aaggatatct tgtgtatggg gtgttgactt gctttatcga ataattaccg tacctgtaat
     1021 tggcttgctg gatatagcgg tagtctaata tctagcaaaa atcttttggg tgaaaaggct
     1081 tgcaatttca cgacaccgaa ctatttgtca ttttttaata aggaagtttt ccataaattc
     1141 ctgtaattct cggttgatct aattgaaaag agtagttttg catcacgatg aggagggctt
     1201 ttgtagaaag aaatacgaac gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc
     1261 ccttacctga agagtcgaat tttattgatg aacttataac ttccaagcat gcaaaccaaa
     1321 agggagaaca agtaatccaa gtagacacgg gaattggatt cttggatcac atgtatcatg
     1381 cactggctaa acatgcaggc tggagcttac gactttactc aagaggtgat ttaatcatcg
     1441 atgatcatca cactgcagaa gatactgcta ttgcacttgg tattgcattc aagcaggcta
     1501 tgggtaactt tgccggcgtt aaaagatttg gacatgctta ttgtccactt gacgaagctc
     1561 tttctagaag cgtagttgac ttgtcgggac ggccctatgc tgttatcgat ttgggattaa
     1621 agcgtgaaaa ggttggggaa ttgtcctgtg aaatgatccc tcacttacta tattcctttt
     1681 cggtagcagc tggaattact ttgcatgtta cctgcttata tggtagtaat gaccatcatc
     1741 gtgctgaaag cgcttttaaa tctctggctg ttgccatgcg cgcggctact agtcttactg
     1801 gaagttctga agtcccaagc acgaagggag tgttgtaaag atgaattgga ttatgtcagg
     1861 aaaagaacga caattttgca tccaaattgt ctaaatttta gagttgcttg aaaacaatag
     1921 aaccttactt gctttataat tacgttaatt agaagcgtta tctcgtgaag gaatatagta
     1981 cgtagccgta taaattgaat tgaatgttca gcttatagaa tagagacact ttgctgttca
     2041 atgcgtcgtc acttaccata ctcactttat tatacgactt taagtataaa ctccgcggtt
     2101 atggtaaaat taatgatgca caaacgtccg attccatatg ggtacactac aattaaatac
     2161 ttttaagtaa cgctgatttc ttatttttta tccttggtgt atttattgtt tataccgcta
     2221 gggcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     2281 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2341 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2401 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2461 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2521 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2581 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2641 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2701 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     2761 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     2821 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     2881 cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc ccgatcagaa
     2941 aattatcgcc ataaaagaca gaataagtca tcagcggttg tttcatttcc tatatttttt
     3001 ttttattttt ttatttttta ataagggaaa atttaacgtc taaggataca gaagattgtt
     3061 agcacattaa agtaataaag gcttaagtag taagtgcctt agcatgttat tgtatttcaa
     3121 aggacataat ctaaaataat aacaatatca tttctcacaa gttattcaat tttctttttt
     3181 ttttctaata atatcaagaa tgtattattt gtttgacata agtcaactaa tttatttaat
     3241 atgctggatt aatcttgcag acatgtaaat taacaagttt tagtcaaata acgttgaagt
     3301 ttcaatgaac tcaaataatt tctctttttt tttatataac tctaatctga tttatatttt
     3361 ccgcagggat caactgaagt tatgacattt ggattggatc acttataacc ttggtcgcca
     3421 aataatacaa aaatcagcgt tataaaacaa agaaggtttt tgttaagaaa ttaatcctct
     3481 ttcttgataa gaaagttgaa ccgaaattgc agatactgat atatgaaaat aatacccaca
     3541 attttgggaa tagcgcaagc ctcaatttaa acaataggtg aggacacatg ataatgacct
     3601 caatgattgt tagaagaaaa gagcctcatt acaaaatcga aaaatgaatg gttgggtaca
     3661 agtttccaaa acatggtaaa gtggactttg cgtatgagac gtaaatagaa aaaaacactt
     3721 gttatatgtt ttctagaatt attgttgtct ctttatggtt ggatgatgca aaatagtaat
     3781 ttcggttagt tgctgtaaaa caccacgaga caaatagata tggatattta ttaaatcagg
     3841 aaaaacgtaa ctctcggcta ctggatggtt cagtcaccca acgattactg gggagagaaa
     3901 acagggcaaa agcaaagctt aaaggaatcc gattgtcatt cggcaatgtg cagcgaaact
     3961 aaaaaccgga taatggacct gttaatcgaa acattgaaga taaaggaaga ggaatcctgg
     4021 catatcatca attgaataag ttgaattaat tatttcaatc tcattctcac tttctgactt
     4081 atagtcgctt tgttaaagct agcatggttt ctaagggtga agaactcttt actggtgtcg
     4141 ttcctatctt ggttgaactt gatggagatg ttaatggaca caagttttca gtctctggtg
     4201 aaggagaagg tgatgctact tatggtaagc ttacccttaa attcatttgc actactggaa
     4261 aacttcctgt cccttggcct actcttgtca ctaccctgtc ttggggtgtt caatgctttg
     4321 ctcgttatcc tgaccatatg aagcaacacg atttctttaa gagtgctatg cctgaaggtt
     4381 atgttcaaga gcgtactatt ttcttcaaag acgatggtaa ctataagact cgtgctgaag
     4441 ttaaatttga gggtgatact ttagttaacc gtattgagct caaaggaatt gattttaaag
     4501 aggatggtaa tattcttggt cataaattag aatacaatta cttttctgat aatgtttaca
     4561 ttactgccga taaacaaaag aatggtatca aggctaactt caagattcgt cataacattg
     4621 aagatggcgg agtacaactt gctgatcatt accaacaaaa cactcctatt ggtgacggtc
     4681 ctgttttgtt gcctgataac cattatcttt ctactcaatc caaattgtct aaagatccta
     4741 atgaaaaacg tgatcacatg gtccttttgg aatttgttac cgctgccggt attaccttgg
     4801 gtatggatga attgtacaag agatcttaac gcgccacttc taaataagcg aatttcttat
     4861 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     4921 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     4981 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggcagat
     5041 ccgctaggga taacagggta atatgtttag cttgcctcgt ccccgccggg tcacccggcc
     5101 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     5161 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     5221 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct gcagacctgc
     5281 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
     5341 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     5401 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaccatg ggtaaggaaa
     5461 agactcacgt ttcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     5521 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
     5581 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     5641 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     5701 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggc aaaacagcat
     5761 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     5821 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     5881 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     5941 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc
     6001 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     6061 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     6121 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     6181 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     6241 tcgatgagtt tttctaatca gtactgacaa taaaaagatt cttgttttca agaacttgtc
     6301 atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta
     6361 tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat
     6421 atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg
     6481 ccgccatcca gtttaaacga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     6541 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6601 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6661 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6721 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6781 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6841 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6901 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6961 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7021 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7081 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7141 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7201 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7261 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7321 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7381 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7441 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7501 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7561 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7621 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7681 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7741 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7801 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7861 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7921 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7981 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8041 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8101 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8161 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8221 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8281 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8341 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8401 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8461 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8521 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8581 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8641 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8701 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8761 cacgaggccc tttcgtc
//
