LOCUS       pDEST-mScarlet-S        9061 bp DNA     circular SYN 26-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   James
  TITLE     Direct Submission
  JOURNAL   Exported Feb 26, 2024 from SnapGene Viewer 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pDEST-mScarlet-SH3PXD2A
FEATURES             Location/Qualifiers
     source          1..9061
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMVF
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             923..1618
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     protein_bind    1640..1664
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1682..4996
                     /codon_start=1
                     /product="Homo sapiens SH3 and PX domains 2A (SH3PXD2A), 
                     transcript variant 1, mRNA 
                     NCBI Reference Sequence: NM_014631.3 "
                     /label=SH3PXD2A
                     /translation="MLAYCVQDATVVDVEKRRNPSKHYVYIINVTWSDSTSQTIYRRYS
                     KFFDLQMQLLDKFPIEGGQKDPKQRIIPFLPGKILFRRSHIRDVAVKRLKPIDEYCRAL
                     VRLPPHISQCDEVFRFFEARPEDVNPPKEDYGSSKRKSVWLSSWAESPKKDVTGADATA
                     EPMILEQYVVVSNYKKQENSELSLQAGEVVDVIEKNESGWWFVSTSEEQGWVPATYLEA
                     QNGTRDDSDINTSKTGEEEKYVTVQPYTSQSKDEIGFEKGVTVEVIRKNLEGWWYIRYL
                     GKEGWAPASYLKKAKDDLPTRKKNLAGPVEIIGNIMEISNLLNKKASGDKETPPAEGEG
                     HEAPIAKKEISLPILCNASNGSAVGVPDRTVSRLAQGSPAVARIAPQRAQISSPNLRTR
                     PPPRRESSLGFQLPKPPEPPSVEVEYYTIAEFQSCISDGISFRGGQKAEVIDKNSGGWW
                     YVQIGEKEGWAPASYIDKRKKPNLSRRTSTLTRPKVPPPAPPSKPKEAEEGPTGASESQ
                     DSPRKLKYEEPEYDIPAFGFDSEPELSEEPVEDRASGERRPAQPHRPSPASSLQRARFK
                     VGESSEDVALEEETIYENEGFRPYAEDTLSARGSSGDSDSPGSSSLSLTRKNSPKSGSP
                     KSSSLLKLKAEKNAQAEMGKNHSSASFSSSITINTTCCSSSSSSSSSLSKTSGDLKPRS
                     ASDAGIRGTPKVRAKKDADANAGLTSCPRAKPSVRPKPFLNRAESQSQEKMDISTLRRQ
                     LRPTGQLRGGLKGSKSEDSELPPQTASEAPSEGSRRSSSDLITLPATTPPCPTKKEWEG
                     PATSYMTCSAYQKVQDSEISFPAGVEVQVLEKQESGWWYVRFGELEGWAPSHYLVLDEN
                     EQPDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNTVNQSKKATPPIPSKPPGGFG
                     KTSGTPAVKMRNGVRQVAVRPQSVFVSPPPKDNNLSCALRRNESLTATDGLRGVRRNSS
                     FSTARSAAAEAKGRLAERAASQGSDSPLLPAQRNSIPVSPVRPKPIEKSQFIHNNLKDV
                     YVSIADYEGDEETAGFQEGVSMEVLERNPNGWWYCQILDGVKPFKGWVPSNYLEKKN"
     protein_bind    complement(5010..5034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5068..5085)
                     /label=BGHR
     polyA_signal    5074..5298
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5344..5772
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5786..6116
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5967..6102
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6164..6211
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6230..6604
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6734..6855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6904..6920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6928..6944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6952..6958,6959..6976,6977..6982))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6997..7018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7306..7894)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8065..8856,8857..8925))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8926..9030)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccatggtgag caagggcgag gcagtgatca aggagttcat
      961 gcggttcaag gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     1021 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     1081 ccccctgccc ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt
     1141 caccaagcac cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa
     1201 gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc
     1261 cctggaggac ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga
     1321 cggccccgta atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc
     1381 cgaggacggc gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg
     1441 ctacctggcg gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc
     1501 ctacaacgtc gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga
     1561 acagtacgaa cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagat
     1621 cgatggtggc tctggaggta caagtttgta caaaaaagca gaagggccgt caaggcccac
     1681 catgctcgcc tactgcgtgc aggatgccac cgtggtggac gtggagaagc ggaggaaccc
     1741 ctccaagcac tacgtataca taatcaatgt gacctggtct gactccacct cccagactat
     1801 ctaccggagg tacagcaagt tctttgacct gcagatgcag cttttggata agtttcccat
     1861 tgaaggtggc cagaaggacc ccaagcaaag gatcatcccc ttcctcccag gcaagatcct
     1921 cttccgcaga agccacatcc gggacgtagc tgtgaagaga ctgaagccca tcgatgaata
     1981 ctgccgggca cttgtccggc tgccccccca catctcacag tgtgacgaag tcttccggtt
     2041 cttcgaggct cgacccgagg atgtcaaccc tccaaaagag gactatggca gttccaagag
     2101 gaaatcagtg tggctgtcca gctgggctga gtcgcccaag aaggacgtga caggtgccga
     2161 cgccaccgcc gagcccatga tcctggaaca gtacgtggtg gtgtccaact ataagaagca
     2221 ggagaactcg gagctgagcc tccaggccgg ggaggtggtg gatgtcatcg agaagaacga
     2281 gagcggctgg tggttcgtga gcacttctga ggagcagggc tgggtccctg ccacctacct
     2341 ggaggcccag aatggtactc gggatgactc cgacatcaac acctctaaga ctggagaaga
     2401 ggagaagtat gtcaccgtgc agccttacac cagccaaagc aaggacgaga ttggctttga
     2461 gaagggcgtc acagtggagg tgatccggaa gaatctggaa ggctggtggt atatcagata
     2521 cctgggcaaa gagggctggg cgccagcatc ctacctgaag aaggccaagg atgacctgcc
     2581 aacccggaag aagaacctgg ccggcccagt ggagatcatt gggaacatca tggagatcag
     2641 caacctgctg aacaagaagg cgtctgggga caaggaaact ccaccagccg aaggcgaggg
     2701 ccatgaggcc cccattgcca agaaggagat cagcctgccc atcctctgca atgcctccaa
     2761 tggcagtgcc gtgggcgttc ctgacaggac tgtctccagg ctggcccagg gctctccagc
     2821 tgtggccagg attgcccctc agcgggccca gatcagctcc ccgaacctac ggacaagacc
     2881 tccaccacgc agagaatcca gcctggggtt ccaactgcca aagccaccag agcccccttc
     2941 tgttgaggtg gagtactaca ccattgccga attccagtcg tgcatttccg atggcatcag
     3001 ctttcggggt ggacagaagg cagaggtcat tgataagaac tcaggtggct ggtggtacgt
     3061 gcagatcggt gagaaggagg gctgggcccc cgcatcatac atcgataagc gcaagaagcc
     3121 caacctgagc cgccgcacaa gcacgctgac ccggcccaag gtgcccccgc cagcaccccc
     3181 cagcaagccc aaggaggccg aggagggccc tacgggggcc agtgagagcc aggactcccc
     3241 gcggaagctc aagtatgagg agcctgagta tgacatccct gcattcggct ttgactcaga
     3301 gcctgagctg agcgaggagc ccgtggagga cagagcctca ggggagaggc ggcctgccca
     3361 gccccaccgg ccctcgccgg cctcttctct gcagcgggcc cgcttcaagg tgggtgagtc
     3421 ttcagaggat gtggccctgg aagaggagac catctatgag aatgagggct tccggccata
     3481 tgcagaggac accctgtcag ccagaggctc ctccggggac agcgactccc caggcagctc
     3541 ctcgctgtcc ctgaccagga aaaactcccc caaatcaggc tcccccaagt catcatcact
     3601 cctaaagctc aaggcagaga agaatgccca ggcagaaatg gggaagaacc actcctcagc
     3661 ctccttttcc tcatccatca ccatcaacac cacttgctgc tcctcctctt cctcctcctc
     3721 ctcttccttg tccaaaacca gtggcgacct gaagccccgc tctgcttcgg acgcaggcat
     3781 ccgcggcact cccaaggtca gggcaaagaa ggatgctgat gcgaacgctg ggctgacctc
     3841 ctgtccccgg gccaagccat cggtccggcc caagccattc ctaaaccgag cagagtcgca
     3901 gagccaagag aagatggaca tcagcacttt acggcgccag ctgagaccca caggccagct
     3961 ccgtggaggg ctcaagggct ccaagagtga ggattcggag ctgcccccgc agacggcctc
     4021 cgaggctccc agtgaggggt ctaggagaag ctcatccgac ctcatcaccc tcccagccac
     4081 cactccccca tgtcccacca agaaggaatg ggaagggcca gccacctcgt acatgacatg
     4141 cagcgcctac cagaaggtcc aggactcgga gatcagcttc cccgcgggcg tggaggtgca
     4201 ggtgctggag aagcaggaga gcgggtggtg gtatgtgagg tttggggagc tggagggctg
     4261 ggccccttcc cactatttgg tgctggatga gaacgagcaa cctgacccct ctggcaaaga
     4321 gctggacaca gtgcccgcca agggcaggca gaacgaaggc aaatcagaca gcctggagaa
     4381 gatcgagagg cgcgtccaag cactgaacac cgtcaaccag agcaagaagg ccacgccccc
     4441 catcccctcc aaacctcccg ggggcttcgg caagacctca ggcactccag cggtgaagat
     4501 gaggaacgga gtgcggcagg tggcggtcag gccccagtcg gtgtttgtgt ccccgccacc
     4561 caaggacaac aacctgtcct gcgccctgcg gaggaatgag tcactcacgg ccactgatgg
     4621 cctccgaggc gtccgacgga actcctcctt tagcactgct cgctccgctg ccgccgaggc
     4681 caagggccgc ctggccgaac gggctgccag ccagggttca gactcacccc tactgcccgc
     4741 ccagcgcaac agcatacccg tgtcccctgt gcgccccaag cccatcgaga agtctcagtt
     4801 catccacaat aacctcaaag atgtgtacgt ctctatcgca gactacgagg gggatgagga
     4861 gacagcaggc ttccaggagg gggtgtccat ggaggttctg gagaggaacc ctaatggctg
     4921 gtggtactgc cagatcctgg atggtgtgaa gcccttcaaa ggctgggtgc cttccaacta
     4981 ccttgagaaa aagaactcag gcctcatggg cccagctttc ttgtacaaag tggttgtcta
     5041 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5101 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5161 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5221 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5281 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     5341 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5401 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5461 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat
     5521 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5581 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5641 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5701 tataagggat tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5761 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5821 cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5881 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5941 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6001 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6061 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6121 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     6181 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     6241 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     6301 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     6361 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     6421 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     6481 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     6541 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     6601 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6661 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6721 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6781 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6841 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6901 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6961 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7021 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7081 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7141 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7201 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7261 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7321 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7381 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7441 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7501 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7561 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7621 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7681 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7741 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7801 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7861 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7921 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7981 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8041 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8101 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8161 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8221 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8281 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8341 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8401 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8461 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8521 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8581 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8641 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8701 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8761 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8821 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8881 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8941 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9001 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9061 c
//
