LOCUS       Exported               11022 bp ds-DNA     circular SYN 19-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   PCMI
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    294..313
                     /label=SV40 polyA R(1)
                     /note="0"
     promoter        398..502
                     /label=AmpR promoter
     CDS             503..1363
                     /label=AmpR
     rep_origin      1534..2119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2505..2521
                     /label=M13 R
                     /note="0"
     LTR             2650..3283
                     /label=3' LTR
     misc_feature    3491..4079
                     /label=WPRE
     CDS             complement(4093..4692)
                     /label=PuroR
     promoter        4701..5030
                     /label=SV40 promoter
     misc_feature    4769..4788
                     /label=SV40 promoter F
                     /note="0"
     CDS             complement(5041..5787)
                     /label=Tet-On 3G
     promoter        5806..6316
                     /label=hPGK promoter
     primer_bind     6200..6221
                     /label=GFP TIDE Primer #2F
     promoter        6336..6700
                     /label=TRE3Gs promoter
     primer_bind     6587..6608
                     /label=GFP TIDE Primer #3F
     primer_bind     6674..6695
                     /label=GFP TIDE Primer #1F
     primer_bind     6686..6720
                     /label=pLVX_APEX-GalT_GFP_F
     misc_feature    6701..6705
                     /label=MCS
     primer_bind     6707..6736
                     /label=F_APEX-GalT_GFP
     regulatory      6710..6719
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6716..7465
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="MGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAG
                     TFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVA
                     VEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGH
                     TIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVD
                     KYAADEDAFFADYAEAHQKLSELGFADA"
     CDS             7472..7498
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     7503..7525
                     /label=F_APEX-GalT_GFP
     CDS             7526..7772
                     /codon_start=1
                     /label=GalT
                     /translation="GRLREPLLSGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGR
                     DLSRLPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGA"
     regulatory      7787..7796
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             7793..8512
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     7796..7819
                     /label=Primer 1
     primer_bind     complement(8509..8542)
                     /label=pLVX_APEX-GalT_GFP_R
     misc_feature    8523..8527
                     /label=MCS
     polyA_signal    8702..8836
                     /label=SV40 polyA signal
     misc_feature    8780..8799
                     /label=SV40 polyA R
                     /note="0"
     misc_feature    8879..8995
                     /label=cPPT/CTS
     misc_feature    8951..8970
                     /label=pLJM R
                     /note="0"
     misc_feature    9487..9720
                     /label=RRE
     misc_feature    10217..10342
                     /label=Packaging Signal
     LTR             10388..11022
                     /label=5' LTR
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg
       61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
      121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt
      181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
      241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga
      361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc
     2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt
     3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     3361 gtgactggaa aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt
     3421 ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc gactgcagaa
     3481 ttaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     3541 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     3601 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     3661 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac
     3721 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     3781 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     3841 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     3901 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     3961 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     4021 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg
     4081 ttccagacgc gttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     4141 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     4201 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     4261 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     4321 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     4381 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     4441 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     4501 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     4561 ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     4621 ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     4681 gtactcggtc atggtcgcgt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     4741 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     4801 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     4861 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4921 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     4981 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attcgcgcgt
     5041 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag
     5101 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc
     5161 ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag
     5221 gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag
     5281 aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc
     5341 ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc
     5401 gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta
     5461 ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag
     5521 agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag
     5581 ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc
     5641 ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag
     5701 tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat
     5761 gactttgctc ttgtccagtc tagacatggt aattcgatga tccccctggg gagagaggtc
     5821 ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc
     5881 ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac
     5941 cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc
     6001 ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt
     6061 gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg
     6121 acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg
     6181 ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt
     6241 cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag
     6301 gcgcaacccc aaccccgtgg aattatcacc tcgagtttac tccctatcag tgatagagaa
     6361 cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc
     6421 agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt
     6481 ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa
     6541 cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttgc ttatgtaaac
     6601 cagggcgcct ataaaagagt gctgattttt tgagtaaact tcaattccac aacacttttg
     6661 tcttatacca actttccgta ccacttccta ccctcgtaaa gaattcgccg ccaccatggg
     6721 aaagtcttac ccaactgtga gtgctgatta ccaggacgcc gttgagaagg cgaagaagaa
     6781 gctcagaggc ttcatcgctg agaagagatg cgctcctcta atgctccgtt tggcattcca
     6841 ctctgctgga acctttgaca agggcacgaa gaccggtgga cccttcggaa ccatcaagca
     6901 ccctgccgaa ctggctcaca gcgctaacaa cggtcttgac atcgctgtta ggcttttgga
     6961 gccactcaag gcggagttcc ctattttgag ctacgccgat ttctaccagt tggctggcgt
     7021 tgttgccgtt gaggtcacgg gtggacctaa ggttccattc caccctggaa gagaggacaa
     7081 gcctgagcca ccaccagagg gtcgcttgcc cgatcccact aagggttctg accatttgag
     7141 agatgtgttt ggcaaagcta tggggcttac tgaccaagat atcgttgctc tatctggggg
     7201 tcacactatt ggagctgcac acaaggagcg ttctggattt gagggtccct ggacctctaa
     7261 tcctcttatt ttcgacaact catacttcac ggagttgttg agtggtgaga aggaaggtct
     7321 ccttcagcta ccttctgaca aggctctttt gtctgaccct gtattccgcc ctctcgttga
     7381 caaatatgca gcggacgaag atgccttctt tgctgattac gctgaggctc accaaaagct
     7441 ttccgagctt gggtttgctg atgccctgca gctgcctccc ctggagcgcc tgaccctgga
     7501 cgtcgagctc aagcttgccg ccaccggaag gcttcgggag ccgctcctga gcggcagcgc
     7561 cgcgatgcca ggcgcgtccc tacagcgggc ctgccgcctg ctcgtggccg tctgcgctct
     7621 gcaccttggc gtcaccctcg tttactacct ggctggccgc gacctgagcc gcctgcccca
     7681 actggtcgga gtctccacac cgctgcaggg cggctcgaac agtgccgccg ccatcgggca
     7741 gtcctccggg gagctccgga ccggaggggc caaggatcca ccggtcgcca ccatggtgag
     7801 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     7861 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     7921 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     7981 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     8041 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     8101 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     8161 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     8221 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     8281 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     8341 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     8401 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     8461 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     8521 cggatccaga ccacctcccc tgcgagctaa gctggacagc caatgacggg taagagagtg
     8581 acatttttca ctaacctaag acaggagggc cgtcagagct actgcctaat ccaaagacgg
     8641 gtaaaagtga taaaaatgta tcactccaac ctaagacagg cgcagcttcc gagggatttg
     8701 agatccagac atgataagat acattgatga gtttggacaa accaaaacta gaatgcagtg
     8761 aaaaaaatgc cttatttgtg aaatttgtga tgctattgcc ttatttgtaa ccattataag
     8821 ctgcaataaa caagttaagt cgataaactg gatctctgct gtccctgtaa taaacccgaa
     8881 aattttgaat ttttgtaatt tgtttttgta gttctttagt ttgtatgtct gttgctatta
     8941 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaggcga
     9001 taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct
     9061 tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat
     9121 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
     9181 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
     9241 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
     9301 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
     9361 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
     9421 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
     9481 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
     9541 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
     9601 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
     9661 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
     9721 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
     9781 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
     9841 atatctcctc ctccaggtct gaagatcagc ggccggccgc ttgctgtgcg gtggtcttac
     9901 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     9961 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
    10021 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
    10081 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
    10141 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
    10201 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
    10261 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
    10321 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
    10381 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
    10441 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
    10501 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
    10561 gagagaccca gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact
    10621 ccccagtccc gcccaggcca cgcctccctg gaaagtcccc agcggaaagt cccttgtagc
    10681 aagctcgata tcagcagttc ttgaagtact ccggatgcag ctctcgggcc acgtgatgaa
    10741 atgctaggcg gctgtcaaac ctccactcta acacttctct ctccgggtca tccatcccat
    10801 gcaggctcac agggtgtaac aagctggtgt tctctccttt attggcctct tctaccttat
    10861 ctggctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat ctgacccctg
    10921 gccctggtgt gtagttctgc taatcaggga agtagccttg tgtgtggtag atccacagat
    10981 caaggatatc ttgtcttctt tgggagtgaa ttagcccttc ca
//
