LOCUS       Exported               10851 bp ds-DNA     circular SYN 22-JUL-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   PCMI
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jan 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    294..313
                     /label=SV40 polyA R(1)
                     /note="0"
     promoter        398..502
                     /label=AmpR promoter
     CDS             503..1363
                     /label=AmpR
     rep_origin      1534..2119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2505..2521
                     /label=M13 R
                     /note="0"
     LTR             2650..3283
                     /label=3' LTR
     misc_feature    3491..4079
                     /label=WPRE
     CDS             complement(4093..4692)
                     /label=PuroR
     promoter        4701..5030
                     /label=SV40 promoter
     misc_feature    4769..4788
                     /label=SV40 promoter F
                     /note="0"
     CDS             complement(5041..5787)
                     /label=Tet-On 3G
     promoter        5806..6316
                     /label=hPGK promoter
     primer_bind     6200..6221
                     /label=GFP TIDE Primer #2F
     promoter        6336..6700
                     /label=TRE3Gs promoter
     primer_bind     6587..6608
                     /label=GFP TIDE Primer #3F
     primer_bind     6674..6695
                     /label=GFP TIDE Primer #1F
     primer_bind     6686..6739
                     /label=In-Fusion.FOR
     misc_feature    6701..6705
                     /label=MCS
     CDS             6710..6745
                     /codon_start=1
                     /label=LYN_Motif
                     /translation="MGCIKSKGKDSA"
     misc_feature    6746..6775
                     /label=Linker
     CDS             6776..7489
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    7490..7525
                     /label=Linker
     CDS             7526
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation=""
     CDS             7541..7564
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7565..8311
                     /codon_start=1
                     /label=APEX2
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             8312..8347
                     /codon_start=1
                     /label=NES
                     /translation="LQLPPLERLTLD"
     primer_bind     complement(8333..8371)
                     /label=In-Fusion.REV
     misc_feature    8352..8356
                     /label=MCS
     polyA_signal    8531..8665
                     /label=SV40 polyA signal
     misc_feature    8609..8628
                     /label=SV40 polyA R
                     /note="0"
     misc_feature    8708..8824
                     /label=cPPT/CTS
     misc_feature    8780..8799
                     /label=pLJM R
                     /note="0"
     misc_feature    9316..9549
                     /label=RRE
     misc_feature    10046..10171
                     /label=Packaging Signal
     LTR             10217..10851
                     /label=5' LTR
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg
       61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
      121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt
      181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
      241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga
      361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc
     2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt
     3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     3361 gtgactggaa aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt
     3421 ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc gactgcagaa
     3481 ttaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     3541 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     3601 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     3661 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac
     3721 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     3781 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     3841 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     3901 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     3961 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     4021 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg
     4081 ttccagacgc gttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     4141 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     4201 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     4261 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     4321 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     4381 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     4441 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     4501 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     4561 ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     4621 ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     4681 gtactcggtc atggtcgcgt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     4741 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     4801 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     4861 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4921 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     4981 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attcgcgcgt
     5041 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag
     5101 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc
     5161 ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag
     5221 gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag
     5281 aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc
     5341 ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc
     5401 gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta
     5461 ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag
     5521 agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag
     5581 ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc
     5641 ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag
     5701 tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat
     5761 gactttgctc ttgtccagtc tagacatggt aattcgatga tccccctggg gagagaggtc
     5821 ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc
     5881 ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac
     5941 cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc
     6001 ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt
     6061 gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg
     6121 acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg
     6181 ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt
     6241 cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag
     6301 gcgcaacccc aaccccgtgg aattatcacc tcgagtttac tccctatcag tgatagagaa
     6361 cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc
     6421 agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt
     6481 ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa
     6541 cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttgc ttatgtaaac
     6601 cagggcgcct ataaaagagt gctgattttt tgagtaaact tcaattccac aacacttttg
     6661 tcttatacca actttccgta ccacttccta ccctcgtaaa gaattcacca tgggatgtat
     6721 aaaatcaaaa gggaaagaca gcgcggatag tgctggtagt gctggtagtg ctggtgtgag
     6781 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     6841 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     6901 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6961 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     7021 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     7081 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     7141 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     7201 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     7261 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     7321 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     7381 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     7441 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag
     7501 aactcgagct caagcttcga attctgcggc cgccaccatg gactacaagg atgacgacga
     7561 taagggaaag tcttacccaa ctgtgagtgc tgattaccag gacgccgttg agaaggcgaa
     7621 gaagaagctc agaggcttca tcgctgagaa gagatgcgct cctctaatgc tccgtttggc
     7681 attccactct gctggaacct ttgacaaggg cacgaagacc ggtggaccct tcggaaccat
     7741 caagcaccct gccgaactgg ctcacagcgc taacaacggt cttgacatcg ctgttaggct
     7801 tttggagcca ctcaaggcgg agttccctat tttgagctac gccgatttct accagttggc
     7861 tggcgttgtt gccgttgagg tcacgggtgg acctaaggtt ccattccacc ctggaagaga
     7921 ggacaagcct gagccaccac cagagggtcg cttgcccgat cccactaagg gttctgacca
     7981 tttgagagat gtgtttggca aagctatggg gcttactgac caagatatcg ttgctctatc
     8041 tgggggtcac actattggag ctgcacacaa ggagcgttct ggatttgagg gtccctggac
     8101 ctctaatcct cttattttcg acaactcata cttcacggag ttgttgagtg gtgagaagga
     8161 aggtctcctt cagctacctt ctgacaaggc tcttttgtct gaccctgtat tccgccctct
     8221 cgttgacaaa tatgcagcgg acgaagatgc cttctttgct gattacgctg aggctcacca
     8281 aaagctttcc gagcttgggt ttgctgatgc cctgcagctg cctcccctgg agcgcctgac
     8341 cctggactaa ggatccagac cacctcccct gcgagctaag ctggacagcc aatgacgggt
     8401 aagagagtga catttttcac taacctaaga caggagggcc gtcagagcta ctgcctaatc
     8461 caaagacggg taaaagtgat aaaaatgtat cactccaacc taagacaggc gcagcttccg
     8521 agggatttga gatccagaca tgataagata cattgatgag tttggacaaa ccaaaactag
     8581 aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat gctattgcct tatttgtaac
     8641 cattataagc tgcaataaac aagttaagtc gataaactgg atctctgctg tccctgtaat
     8701 aaacccgaaa attttgaatt tttgtaattt gtttttgtag ttctttagtt tgtatgtctg
     8761 ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt
     8821 taaaggcgat accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct
     8881 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
     8941 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     9001 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     9061 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     9121 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     9181 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     9241 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
     9301 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
     9361 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
     9421 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
     9481 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
     9541 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
     9601 ggtgctactc ctaatggttc aatttttact actttatatt tatataattc acttctccaa
     9661 ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccggccgct tgctgtgcgg
     9721 tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta
     9781 tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt
     9841 cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg
     9901 atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta
     9961 tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg
    10021 atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc
    10081 cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg
    10141 cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt
    10201 ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct
    10261 ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct
    10321 ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg
    10381 gtctaaccag agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg
    10441 ctcgccactc cccagtcccg cccaggccac gcctccctgg aaagtcccca gcggaaagtc
    10501 ccttgtagca agctcgatat cagcagttct tgaagtactc cggatgcagc tctcgggcca
    10561 cgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa cacttctctc tccgggtcat
    10621 ccatcccatg caggctcaca gggtgtaaca agctggtgtt ctctccttta ttggcctctt
    10681 ctaccttatc tggctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc
    10741 tgacccctgg ccctggtgtg tagttctgct aatcagggaa gtagccttgt gtgtggtaga
    10801 tccacagatc aaggatatct tgtcttcttt gggagtgaat tagcccttcc a
//
