LOCUS       dsA02_BSP_A                 6130 bp    DNA     circular SYN 06-OCT-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     rep_origin      3150..3738
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      1666..2094
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(4165..4356)
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(join(5165..6130,1..117))
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2213..3028)
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(118..195)
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      1582..1629
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             595..1428
                     /note="pLannotate"
                     /label="HLA-A (fragment)"
                     /database="swissprot"
                     /identity="92.1"
                     /match_length="76.2"
                     /fragment="True"
                     /other="CDS"
     protein_bind    523..547
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     RBS             562..584
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     promoter        504..522
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(3924..4063)
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(4825..5116)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="24.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(203..482)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.5"
                     /fragment="True"
                     /other="CDS"
     promoter        2147..2204
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="56.3"
                     /fragment="True"
                     /other="promoter"
     CDS             4373..4753
                     /note="pLannotate"
                     /label="YPB1_ECOLX"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="96.2"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(5131..5152)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             1525..1574
                     /note="pLannotate"
                     /label="SET1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
       61 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
      121 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
      181 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
      241 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
      301 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
      361 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
      421 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
      481 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
      541 caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgggcagt
      601 catagcatgc gctacttctt caccagcgtg agtcgtcctg gtcgtggcga accgcgcttc
      661 atcgccgttg gttacgttga cgacacccaa ttcgtgcgct tcgatagcga cgcagccagt
      721 cagcgtatgg aaccgcgcgc accgtggatc gagcaggaag gtccggagta ctgggacggc
      781 gaaacccgca aggttaaagc ccacagccag acccatcgtg tggatctggg caccctgcgc
      841 ggctactaca accagagtga ggccggcagc cacaccgtgc aacgcatgta tggctgtgac
      901 gtgggtagcg actggcgctt cctgcgtggc taccatcagt acgcctacga ctgcaaggat
      961 tacatcgccc tgaaagagga cttacgcagc tggacagcag cagacatggc cgcccagacc
     1021 accaagcaca aatgggaagc agcccatgtg gccgagcagc tgcgtgcata tctggagggt
     1081 acctgcgttg agtggctgcg ccgctacctg gagaacggta aggagaccct gcagcgcacc
     1141 gacgcaccga aaacccacat gacccaccac gccgttagcg atcacgaagc cacactgcgc
     1201 tgctgggccc tgagtttcta cccggccgag atcaccctga cctggcaacg tgacggcgag
     1261 gaccagaccc aggacaccga actggttgag acacgccctg ccggtgacgg cacatttcag
     1321 aaatgggccg ccgttgtggt gccgagcggt caagagcagc gctacacctg ccacgtgcag
     1381 catgaaggcc tgccgaagcc tttaaccctg cgctgggagc cgggatccct gcatcatatt
     1441 ctggatgcac agaaaatggt gtggaatcat cgttaaaagc ttgcggccgc actcgagcac
     1501 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     1561 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     1621 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
     1681 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     1741 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     1801 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     1861 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     1921 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     1981 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     2041 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2101 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2161 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa
     2221 actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt
     2281 tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg
     2341 caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt
     2401 tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg
     2461 gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac
     2521 gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag
     2581 cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc
     2641 ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta
     2701 atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag
     2761 tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga
     2821 ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg
     2881 gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc
     2941 gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc
     3001 aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag
     3061 acagttttat tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3121 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3181 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3241 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     3301 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3361 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3421 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3481 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3541 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3601 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3661 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3721 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3781 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3841 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3901 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     3961 atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4021 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
     4081 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4141 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4201 gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc
     4261 atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg
     4321 ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt
     4381 tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac
     4441 tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat
     4501 gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt
     4561 aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca
     4621 gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt
     4681 tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg
     4741 tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag
     4801 gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc
     4861 gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa
     4921 taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat
     4981 gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag
     5041 tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
     5101 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
     5161 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5221 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     5281 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     5341 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     5401 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
     5461 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     5521 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     5581 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     5641 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5701 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5761 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     5821 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     5881 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     5941 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     6001 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     6061 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     6121 ccatcgccgc
//
