LOCUS       Exported                6010 bp DNA     circular SYN 24-JAN-2024
DEFINITION  SAHS4_RAMVA in pCDB179.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 24, 2024 from SnapGene 6.0.5
            https://www.snapgene.com
COMMENT     Resistance markers: Kanamycin
            This file was created using tools provided by Twist Bioscience
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="SAHS4_RAMVA in pCDB179"
     misc_feature    1..462
                     /label=RvSAHS4
                     /label=insert
                     /label=nonstandard type: misc_feature_
     misc_feature    457..462
                     /label=3'
                     /label=nonstandard type: misc_feature_
     misc_feature    463..6010
                     /label=vector_backbone pCDB179
                     /label=vector_backbone-462-6010
                     /label=nonstandard type: misc_feature_
     misc_feature    632..938
                     /label=f1
                     /label=nonstandard type: misc_feature_
     misc_feature    1162..1977
                     /label=kan
                     /label=nonstandard type: misc_feature_
     misc_feature    2099..2687
                     /label=colE1_high_copy
                     /label=nonstandard type: misc_feature_
     misc_feature    3114..3305
                     /label=ROP
                     /label=nonstandard type: misc_feature_
     misc_feature    4080..4101
                     /label=CAP_binding
                     /label=nonstandard type: misc_feature_
     misc_feature    4114..5205
                     /label=lacI
                     /label=nonstandard type: misc_feature_
     misc_feature    5197..5274
                     /label=lacIQ_promoter
                     /label=nonstandard type: misc_feature_
     misc_feature    5587..5605
                     /label=T7
                     /label=nonstandard type: misc_feature_
     misc_feature    5604..5632
                     /label=lacO
                     /label=nonstandard type: misc_feature_
     misc_feature    5645..5667
                     /label=RBS_3
                     /label=nonstandard type: misc_feature_
     misc_feature    5650..5666
                     /label=T7_transl_en_RBS
                     /label=nonstandard type: misc_feature_
     misc_feature    5675..6010
                     /label=SUMO
     misc_feature    5681..5698
                     /label=6x_His_tag
                     /label=nonstandard type: misc_feature_
ORIGIN
        1 cgccctcatg acgagagcaa ggcccagtgg accggcaaac cttggcttgg taagtgggag
       61 tcgattgatg gcacgcctga aaactgggag gcatttgtga aggcggcgaa catcccccca
      121 aaggaccaag ccctgtacaa cgggaagcag aagaccttgt tgaagtattg gaaagaggcg
      181 ggtgaagacc attatcacgt gcaaacctcc ttccctggga cggaacataa gatggaaacc
      241 agttttaaga tgggccagga aggaactctg tcgcatgatg gcgtcgattt aaaatatgtt
      301 tgtaccgaag atggtgagca gttaataacc aaaatcaaca ttccatctaa gaatcaggaa
      361 acaattgtca cttacacagc tacgggtgat gatctcgagc agacgtttac cagcaatggt
      421 gttacgggaa agcgttggta caaaaagatc catgcctaat gatcgaggat ccggctgcta
      481 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      541 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      601 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      661 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      721 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      781 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
      841 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
      901 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
      961 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     1021 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     1081 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1141 tatccgctca tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt
     1201 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     1261 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     1321 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     1381 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt
     1441 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     1501 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
     1561 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     1621 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
     1681 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     1741 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     1801 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     1861 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     1921 catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac
     1981 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgacc aaaatccctt
     2041 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2101 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2161 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2221 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     2281 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2341 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2401 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2461 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2521 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2581 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2641 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2701 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2761 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2821 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     2881 ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac
     2941 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     3001 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3061 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3121 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     3181 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     3241 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     3301 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     3361 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     3421 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
     3481 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
     3541 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
     3601 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
     3661 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
     3721 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc
     3781 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     3841 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     3901 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     3961 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     4021 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     4081 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4141 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4201 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     4261 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     4321 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     4381 tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     4441 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     4501 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
     4561 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     4621 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     4681 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     4741 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     4801 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     4861 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     4921 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     4981 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     5041 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     5101 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     5161 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
     5221 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
     5281 ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     5341 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     5401 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     5461 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     5521 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg
     5581 cgaaattaat acgactcact atagggaaat tgtgagcgga taacaattcc cctctagaaa
     5641 taattttgtt taactttaag aaggagatat acatatgggt caccaccacc accaccacca
     5701 ccaccaccac ggtagcctgc aagattcgga agtcaaccaa gaagcaaagc cggaagtcaa
     5761 gccggaagtg aaaccggaaa cccatattaa cctgaaagtt agtgacggca gctctgaaat
     5821 tttctttaag atcaaaaaga ccacgccgct gcgtcgcctg atggaagcgt ttgccaaacg
     5881 tcagggcaag gaaatggata gcctgcgttt cctgtatgac ggtattcgca tccaggcaga
     5941 tcaagcgccg gaagacctgg acatggaaga caacgacatc attgaagccc accgtgaaca
     6001 gattggcggc
//
