LOCUS       Exported                5382 bp ds-DNA     circular SYN 24-JAN-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Chris Mehrer
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 24, 2024 from SnapGene 5.2.5
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     source          86..221
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          86..221
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          86..221
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          86..221
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          223..225
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          223..225
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          223..225
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          223..225
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          1344..1472
                     /note="color: #ffffff"
     source          1344..1472
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60"
     source          1573..1701
                     /note="color: #ffffff"
     source          1573..1701
                     /organism="Vitreoscilla sp."
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60"
     source          1724..2917
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          1724..2917
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          1724..2917
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     source          1724..2917
                     /organism="Expression vector pZE1PLlacO-1 luc"
                     /host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:60475"
     primer_bind     35..52
                     /label=gCRM135
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(67..115)
                     /label=gCRM259
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     86..107
                     /label=gCRM258
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    109..162
                     /label=ArcA Terminator
                     /note="color: #a6acb3"
     primer_bind     complement(203..242)
                     /label=gCRM216
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     213..258
                     /label=gCRM215
                     /note="color: #a020f0; direction: RIGHT"
     CDS             complement(223..1317)
                     /codon_start=1
                     /label=Fdh Cb
                     /label=Fdh Cd
                     /note="color: #993366"
                     /translation="MKIVLVLYDAGKHAADEEKLYGCTENKLGIANWLKDQGHELITTS
                     DKEGETSELDKHIPDADIIITTPFHPAYITKERLDKAKNLKLVVVAGVGSDHIDLDYIN
                     QTGKKISVLEVTGSNVVSVAEHVVMTMLVLVRNFVPAHEQIINHDWEVAAIAKDAYDIE
                     GKTIATIGAGRIGYRVLERLLPFNPKELLYYDYQALPKEAEEKVGARRVENIEELVAQA
                     DIVTVNAPLHAGTKGLINKELLSKFKKGAWLVNTARGAICVAEDVAAALESGQLRGYGG
                     DVWFPQPAPKDHPWRDMRNKYGAGNAMTPHYSGTTLDAQTRYAEGTKNILESFFTGKFD
                     YRPQDIILLNGEYVTKAYGKHDKK"
     primer_bind     complement(493..504)
                     /label=rCRM083
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     723..744
                     /label=rCRM133
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(771..787)
                     /label=rCRM134
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(1039..1049)
                     /label=rCRM075
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(1290..1343)
                     /label=gCRM261
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     1318..1376
                     /label=gCRM260
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    complement(1318..1343)
                     /label=RBS Salis v1.1 57567.48
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(1344..1472)
                     /label=pVHb promoter
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     1344..1376
                     /label=gCRM420
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     1344..1376
                     /label=gCRM225
                     /note="color: #a020f0; direction: RIGHT"
     regulatory      1381..1387
                     /regulatory_class="other"
                     /label=promoter 1
                     /note="promoter 1"
                     /note="color: #ffffff"
     misc_feature    1387
                     /label=+1
                     /note="color: #a6acb3"
     regulatory      1394..1400
                     /regulatory_class="minus_10_signal"
                     /note="color: #ffffff"
     regulatory      1412..1418
                     /regulatory_class="minus_35_signal"
                     /note="color: #ffffff"
     misc_feature    1422..1426
                     /label=fnr binding
                     /note="color: #a6acb3"
     misc_feature    1431..1435
                     /label=fnr binding
                     /note="color: #a6acb3"
     regulatory      1433..1440
                     /regulatory_class="other"
                     /label=promoter 2
                     /note="promoter 2"
                     /note="color: #ffffff"
     primer_bind     complement(1454..1502)
                     /label=gCRM263
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(1454..1472)
                     /label=gCRM222
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     1473..1488
                     /label=gCRM262
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     1495..1517
                     /label=rCRM130
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     1543..1591
                     /label=gCRM222
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(1555..1572)
                     /label=gCRM221
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    1573..1701
                     /label=pVHb promoter
                     /note="color: #a6acb3"
     primer_bind     1573..1591
                     /label=gCRM263
                     /note="color: #a020f0; direction: RIGHT"
     regulatory      1605..1612
                     /regulatory_class="other"
                     /label=promoter 2
                     /note="promoter 2"
                     /note="color: #ffffff"
     misc_feature    1610..1614
                     /label=fnr binding
                     /note="color: #a6acb3"
     misc_feature    1619..1623
                     /label=fnr binding
                     /note="color: #a6acb3"
     regulatory      1627..1633
                     /regulatory_class="minus_35_signal"
                     /note="color: #ffffff"
     regulatory      1645..1651
                     /regulatory_class="minus_10_signal"
                     /note="color: #ffffff"
     regulatory      1658..1664
                     /regulatory_class="other"
                     /label=promoter 1
                     /note="promoter 1"
                     /note="color: #ffffff"
     misc_feature    1658
                     /label=+1
                     /note="color: #a6acb3"
     primer_bind     complement(1669..1728)
                     /label=gCRM420
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(1669..1701)
                     /label=gCRM260
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(1669..1701)
                     /label=gCRM225
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     1699..1745
                     /label=gCRM419
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    1702..1723
                     /label=RBS Salis v2.0 58437.39
                     /note="color: #a6acb3"
     CDS             1724..2917
                     /codon_start=1
                     /label=tdTER
                     /note="color: #993366"
                     /translation="MIVKPMVRNNICLNAHPQGCKKGVEDQIEYTKKRITAEVKAGAKA
                     PKNVLVLGCSNGYGLASRITAAFGYGAATIGVSFEKAGSETKYGTPGWYNNLAFDEAAK
                     REGLYSVTIDGDAFSDEIKAQVIEEAKKKGIKFDLIVYSLASPVRTDPDTGIMHKSVLK
                     PFGKTFTGKTVDPFTGELKEISAEPANDEEAAATVKVMGGEDWERWIKQLSKEGLLEEG
                     CITLAYSYIGPEATQALYRKGTIGKAKEHLEATAHRLNKENPSIRAFVSVNKGLVTRAS
                     AVIPVIPLYLASLFKVMKEKGNHEGCIEQITRLYAERLYRKDGTIPVDEENRIRIDDWE
                     LEEDVQKAVSALMEKVTGENAESLTDLAGYRHDFLASNGFDVEGINYEAEVERFDRI"
     primer_bind     2918..2938
                     /label=gCRM224
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     complement(2967..2985)
                     /label=pMSB6_seqR_1
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(3022..3040)
                     /label=pTRC99A.seq.REV
                     /note="color: #a020f0; direction: LEFT"
     terminator      3120..3206
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli 
                     rrnBgene"
                     /note="color: #ffffff"
     primer_bind     complement(3140..3159)
                     /label=rCRM096
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     3140..3159
                     /label=rCRM095
                     /note="color: #a020f0; direction: RIGHT"
     terminator      3298..3325
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli 
                     rrnBgene"
                     /note="color: #ffffff"
     promoter        3345..3436
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff"
     CDS             3437..4297
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4015..4035)
                     /label=gCRM018
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     4015..4035
                     /label=gCRM017
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    4452..5071
                     /label=pBR322_origin
                     /note="pBR322_origin"
                     /note="color: #a6acb3"
     rep_origin      4468..5056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    5242..5382
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
ORIGIN
        1 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
       61 gcttacagac aagctgtgac cgtctgacag cttatcatcg actgcacgaa ggttgaaaaa
      121 taaaaacggc gctaaaaagc gccgtttttt ttgacggtgg tagtgcacca atgcttctgg
      181 cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tattattttt tatcatgttt
      241 accatacgct ttggtcacat attcgccgtt cagcagaatg atatcctgcg gacgataatc
      301 aaatttgccg gtaaaaaagc tttccaggat atttttggtg ccttccgcat aacgggtctg
      361 cgcatctaaa gtggtacccg agtaatgcgg tgtcatcgca ttaccagcgc catatttatt
      421 acgcatatcg cgccacggat gatcttttgg agctggttgc ggaaaccaga catcgccgcc
      481 gtaaccacgc agctgaccgc tttcaagcgc ggctgccaca tcttccgcca cgcaaatggc
      541 accgcgcgcg gtattaacca gccaggcacc ctttttaaac ttgctcaaca gctctttgtt
      601 gatcaggccc ttggtgcctg catgcagcgg cgcgttaacg gtgacaatat ctgcctgggc
      661 caccagttct tcaatgtttt cgacacggcg cgcacccact ttctcttcgg cttctttcgg
      721 cagcgcctgg tagtcatagt acagcagttc tttcggattg aacgggagca ggcgttccag
      781 tacgcgatag ccaatgcgtc ccgcgccaat cgtggcaatg gttttacctt cgatatcata
      841 agcatctttt gcgatcgccg ccacttccca gtcatgatta atgatttgct catgggcggg
      901 cacaaagtta cgcaccagta ccagcatggt catcaccaca tgttcagcga cagacacgac
      961 gttgctgccc gtcacttcca gcacgctgat ttttttcccg gtctggttaa tataatccag
     1021 atcgatatgg tcagaaccca cgccagccac aaccactaat ttcaagtttt tagctttgtc
     1081 cagacgttct ttcgtgatat aggccggatg aaacggagtg gttatgataa tatcagcgtc
     1141 cggaatatgt ttatccagtt cagaggtttc gccttcctta tcgcttgtgg taatcagttc
     1201 atggccctga tccttcagcc agttcgcaat gcccagcttg ttttcggtac aaccatacag
     1261 tttttcttca tccgctgcgt gtttgcccgc atcatacaaa accaggacaa ttttcatgtg
     1321 tgcctcccta actacttaat tcataagttc attattatgg ttttgccata catcagtatg
     1381 gtgtagcagc acttattata atctttattg cctcttaaaa cttaatccac atcaaaactc
     1441 aaatactttt aaccccagcg tcctgtaagc ttccgggagc tgcatgtgtc agaggttttc
     1501 accgtcatca ccgaaacgcg cgaggcagca gatcaattcg cgcgcgaagg cgaagcggca
     1561 tgcatttacg ttaagcttac aggacgctgg ggttaaaagt atttgagttt tgatgtggat
     1621 taagttttaa gaggcaataa agattataat aagtgctgct acaccatact gatgtatggc
     1681 aaaaccataa taatgaactt aagacgacaa gggggcgaat ttaatgattg tgaagccgat
     1741 ggtacgcaac aacatttgtc tgaacgcgca cccgcaggga tgcaagaaag gcgtcgaaga
     1801 tcagattgaa tataccaaga agcgtatcac tgcagaggtg aaggccgggg ctaaagcccc
     1861 taaaaatgtc ctggtgctcg gctgtagcaa cggttacggt ctggcaagtc gcatcactgc
     1921 ggctttcggt tacggggccg ctaccattgg cgtctcgttt gaaaaggcag gtagcgaaac
     1981 taaatatggc actccgggtt ggtataataa cctggcattt gacgaagccg cgaaacgcga
     2041 aggtttatat tcagtgacga ttgatggcga cgcgttcagc gacgaaatca aagcgcaggt
     2101 gatcgaggaa gcaaagaaga aaggtatcaa atttgatctg atcgtgtact cgctggcatc
     2161 gccggttcgc accgacccgg ataccggaat tatgcacaaa tctgtgctga agccgtttgg
     2221 taaaaccttt acaggtaaaa ccgtggatcc gtttactgga gagcttaaag agatctcggc
     2281 agaacccgcg aatgatgagg aagcagcggc gaccgtgaaa gtgatgggag gcgaagattg
     2341 ggaacgctgg atcaaacaac tgtcgaaaga aggcctgtta gaagagggct gcattacact
     2401 tgcgtatagt tacatcggtc cggaagcgac ccaagcactc tatcgtaagg gtactatcgg
     2461 taaagcgaaa gaacaccttg aagcgactgc acaccgcctg aacaaagaaa acccgtctat
     2521 ccgtgcgttc gtcagcgtaa acaaaggttt ggttacgcgt gcgagcgcgg ttatccctgt
     2581 tattccgctg tatttggcca gtttgtttaa agttatgaag gaaaaaggca accatgaagg
     2641 gtgcattgaa cagattacac gcctttacgc cgagcgcctg tatcgtaaag atggaacgat
     2701 ccctgtggat gaagaaaacc gcattcgcat cgatgattgg gaattggaag aggatgtgca
     2761 gaaggccgtt tcggccctga tggaaaaagt gaccggcgaa aacgccgaat ctttaactga
     2821 tctcgccggg taccgccatg attttctggc aagcaacggt ttcgatgtgg agggtattaa
     2881 ctatgaggcg gaggttgaac gcttcgatcg catttaaggc tgttttggcg gatgagagaa
     2941 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
     3001 gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
     3061 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
     3121 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     3181 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
     3241 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
     3301 aggccatcct gacggatggc ctttttgcgt ttctacaaac tctttttgtt tatttttcta
     3361 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3421 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3481 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3541 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3601 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3661 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3721 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3781 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3841 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3901 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3961 gcgtgacacc acgatgccta cagcaatggc aacaacgttg cgcaaactat taactggcga
     4021 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4081 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4141 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4201 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4261 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4321 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4381 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4441 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4501 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4561 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     4621 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4681 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4741 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4801 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4861 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4921 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4981 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5041 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5101 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5161 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5221 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     5281 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5341 gtatacactc cgctatcgct acgtgactgg gtcatggctg cg
//
