LOCUS       BRINDLEY_Z5P_2_p        6990 bp DNA     circular SYN 16-JAN-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 16, 2024 from SnapGene Viewer 7.0.3
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(5395..6990,1..5394)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..783
                     /codon_start=1
                     /label=CHIKV capsid
                     /label=POLSF_CHIKS (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=97.7"
                     /note="/match_length=31.7"
                     /note="/other=CDS"
                     /translation="MEFIPTQTFYNRRYQPRPWTPRPTIQVIRPRPRPQRKAGQLAQLI
                     SAVNKLTMRAVPQQKPRKNRKNKKQKQKQQAPRNNMNQKKQLPKKKPAQKKKKPGRRER
                     MCMKIENDCIFEVKHEGKVTGYACLVGDKVMKPAHVKGTIDNADLAKLAFKRSSKYDLE
                     CAQIPVHMKSDASKFTHEKPEGYYNWHHGAVQYSGGRFTIPTGAGKPGDSGRPIFDNKG
                     RVVAIVLGGANEGARTALSVVTWNKDIVTKITPEGAEEW"
     polyA_signal    830..1054
                     /label=bGH poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     promoter        1058..1079
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=6.1"
                     /note="/other=promoter"
     rep_origin      1100..1528
                     /direction=RIGHT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        1542..1871
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1938..2729
                     /codon_start=1
                     /label=neo
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2753..2911
                     /codon_start=1
                     /label=ble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=98.1"
                     /note="/match_length=42.1"
                     /note="/other=CDS"
                     /translation="MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLML
                     EFFAHPNL"
     polyA_signal    2906..3027
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     protein_bind    3100..3116
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        3124..3154
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    3169..3190
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(3203..3295)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      3478..4066
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(4237..5097)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        5098..5202
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    5261..5313
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=37.6"
                     /note="/other=misc_feature"
     enhancer        5468..5847
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        5848..6051
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          6172..6744
                     /label=beta-globin intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     promoter        6799..6817
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          6868..6908
                     /label=beta-globin intron (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=7.2"
                     /note="/other=intron"
ORIGIN
        1 atggagttta tcccaaccca aactttctac aataggaggt accagcctcg accttggact
       61 ccgcgcccta ctatccaagt tatcagaccc agaccgcgtc cgcaaaggaa agccgggcaa
      121 cttgcccagc tgatctcagc agttaataaa ctgacaatgc gcgcggtacc tcaacagaag
      181 ccgcgcaaga atcggaagaa taagaagcaa aagcaaaagc agcaggcgcc acgaaacaac
      241 atgaatcaaa agaagcagct ccctaaaaag aaaccggctc aaaagaaaaa gaagccgggc
      301 cgtagagaga gaatgtgcat gaaaatcgaa aatgattgca tcttcgaagt caagcatgaa
      361 ggtaaggtaa caggttacgc gtgcttggta ggggacaaag taatgaagcc agcacacgta
      421 aaggggacca tcgataatgc ggacctggcc aaattggcct tcaagcggtc atctaagtac
      481 gaccttgaat gcgcgcagat acccgtgcac atgaagtccg acgcttcgaa gttcacccat
      541 gagaaaccgg aggggtacta caactggcac cacggagcag tacagtactc aggaggccgg
      601 ttcaccatcc ctacaggtgc gggcaaacca ggggacagcg gtagaccgat cttcgacaac
      661 aaggggcgcg tggtggccat agttttagga ggagctaatg aaggagcccg tacagccctc
      721 tcggtggtga cctggaacaa agacatcgtc acgaaaatca cccctgaggg ggccgaagag
      781 tggtgactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
      841 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
      901 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
      961 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     1021 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     1081 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1141 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1201 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1261 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     1321 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     1381 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     1441 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     1501 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     1561 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     1621 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     1681 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     1741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     1801 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     1861 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     1921 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     1981 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2041 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     2101 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     2161 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     2221 gcgaagtgcc ggggcaggat ctcctgtcat cccaccttgc tcctgccgag aaagtatcca
     2281 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     2341 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     2401 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     2461 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     2521 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     2581 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     2641 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     2701 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     2761 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     2821 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     2881 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     2941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3001 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     3061 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3121 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3181 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3241 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3301 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3361 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3601 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3661 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3721 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3781 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3841 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3901 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3961 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4021 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4081 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4141 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4201 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4261 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4321 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4381 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4441 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4501 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4561 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4621 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4681 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4741 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4801 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4861 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4921 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     4981 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5041 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5101 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5161 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5221 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     5281 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     5341 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     5401 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     5461 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     5521 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     5581 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     5641 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     5701 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     5761 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     5821 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     5881 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     5941 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     6001 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     6061 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     6121 gaccgatcca gcctccgggg gatcgatccc cccgatcctg agaacttcag ggtgagtttg
     6181 gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc
     6241 aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca tggaccctca
     6301 tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc tcttattttc
     6361 ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg aatttttaaa
     6421 ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt ttttcaaggc
     6481 aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt tataattaaa
     6541 tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat tcttattggt
     6601 agaaacaact acatcctggt catcatcctg cctttctctt tatggttaca atgatataca
     6661 ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc taaccatgtt
     6721 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     6781 cattttggca aagaattgta atacgactca ctatagggcg aattcggatc cactagtcca
     6841 gtgtggtgga attctgcaga tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     6901 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     6961 tcgagctcat cgatgcatgg taccgccacc
//
