LOCUS       plasmid                 6172 bp    DNA     circular SYN 25-JAN-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2313..3173
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             806..1891
                     /note="pLannotate"
                     /label="Msi1"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             5460..5633
                     /note="pLannotate"
                     /label="lacZα"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             join(6164..6172,1..645)
                     /note="pLannotate"
                     /label="GST26_SCHJA"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             4255..5337
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        2208..2312
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4177..4254
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      3344..3933
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.2"
                     /fragment="False"
                     /other="rep_origin"
     promoter        5386..5416
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        6089..6117
                     /note="pLannotate"
                     /label="tac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    5424..5440
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    6125..6141
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    5350..5371
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     misc_feature    complement(4119..4170)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    2063..2103
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="29.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1922..1961
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5897..6013)
                     /note="pLannotate"
                     /label="yciV (fragment)"
                     /database="swissprot"
                     /identity="84.6"
                     /match_length="13.3"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 atactaggtt attggaaaat taagggcctt gtgcaaccca ctcgacttct tttggaatat
       61 cttgaagaaa aatatgaaga gcatttgtat gagcgcgatg aaggtgataa atggcgaaac
      121 aaaaagtttg aattgggttt ggagtttccc aatcttcctt attatattga tggtgatgtt
      181 aaattaacac agtctatggc catcatacgt tatatagctg acaagcacaa catgttgggt
      241 ggttgtccaa aagagcgtgc agagatttca atgcttgaag gagcggtttt ggatattaga
      301 tacggtgttt cgagaattgc atatagtaaa gactttgaaa ctctcaaagt tgattttctt
      361 agcaagctac ctgaaatgct gaaaatgttc gaagatcgtt tatgtcataa aacatattta
      421 aatggtgatc atgtaaccca tcctgacttc atgttgtatg acgctcttga tgttgtttta
      481 tacatggacc caatgtgcct ggatgcgttc ccaaaattag tttgttttaa aaaacgtatt
      541 gaagctatcc cacaaattga taagtacttg aaatccagca agtatatagc atggcctttg
      601 cagggctggc aagccacgtt tggtggtggc gaccatcctc caaaatcgga tctggaagtt
      661 ctgttccagg gggcccctgg gattaatgga cgaaaaaacc accggttggc gtggtggtca
      721 cgttgttgaa ggtctggctg gtgaactgga acagctgcgt gctcgtctgg aacaccaccc
      781 gcagggtcag cgtgaaccgg gatccatgga gactgacgcg ccccagcccg gcctcgcctc
      841 cccggactcg ccgcacgacc cctgcaagat gttcatcgga ggactcagtt ggcagaccac
      901 gcaggaaggg ctgcgcgaat acttcggcca gttcggggag gtgaaagagt gtctggtgat
      961 gcgggacccc ctgaccaaaa gatccagggg tttcggcttc gtcactttca tggaccaggc
     1021 gggggtggat aaagtgctgg cgcaatcgcg gcacgagctc gactccaaaa caattgaccc
     1081 caaggtggcc tttcctcgaa gagcacagcc taagatggtc actcggacga agaagatctt
     1141 cgtggggggg ctgtctgtga acaccacggt ggaagatgtg aaacactatt tcgagcagtt
     1201 cggaaaggtg gatgatgcca tgctgatgtt cgacaaaacc accaacaggc acagagggtt
     1261 tggatttgtc acgtttgaga gcgaggacat cgtagagaaa gtttgtgaga tccacttcca
     1321 tgaaatcaac aacaaaatgg tggaatgcaa gaaagcccag ccaaaggagg tgatgtcccc
     1381 gacaggctca gcccggggca ggtctcgggt catgccctac ggaatggatg ccttcatgct
     1441 gggtattggg atgctgggtt acccagggtt ccaagccacg acctacgcca gccggagtta
     1501 cacaggcctt gcccctggtt acacctacca gttccccgaa ttccgtgtag agcggagccc
     1561 tctcccgagc gccccagtcc tccccgagct cacagctatc cctctcacgg cttatgggcc
     1621 catggcggcg gcagcggcgg cggcagctgt agttcgaggg acaggctctc acccctggac
     1681 gatggctccc cctccaggtt ccactcccag ccgcacaggg ggcttcctag ggaccacaag
     1741 ccccggcccc atggctgagc tctacggggc agccaaccag gactccgggg tcagcagtta
     1801 catcagcgcc gccagccccg cccccagcac tggtttcggc cacagtcttg ggggtccctt
     1861 gattgccaca gccttcacca atgggtacca ctgagcggcc gcatcgtgac tgactgacga
     1921 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1981 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     2041 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     2101 ataattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2161 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2221 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2281 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2341 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2401 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2461 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2521 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     2581 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2641 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2701 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2761 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2821 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     2881 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2941 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3001 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3061 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3121 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3181 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3241 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3301 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttt
     3361 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3421 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3481 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3541 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3601 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3661 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     3721 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     3781 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     3841 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     3901 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     3961 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4021 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4081 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     4141 acgcatctgt gcggtatttc acaccgcata aattccgaca ccatcgaatg gcgcaaaacc
     4201 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
     4261 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
     4321 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
     4381 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
     4441 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
     4501 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
     4561 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
     4621 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
     4681 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
     4741 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
     4801 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
     4861 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
     4921 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
     4981 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
     5041 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
     5101 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     5161 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
     5221 ctggtgaaaa gaaaaaccac gctagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5281 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     5341 caacgcaatt aatgtaagtt agctcactca ttaggcaccc caggctttac actttatgct
     5401 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     5461 tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     5521 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     5581 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
     5641 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
     5701 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
     5761 acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
     5821 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
     5881 gaattatttt tgatggcgtt ggaattagct tatcgactgc acggtgcacc aatgcttctg
     5941 gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat
     6001 tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg
     6061 gttctggcaa atattctgaa atgagctgtt gacaattaat catcggctcg tataatgtgt
     6121 ggaattgtga gcggataaca atttcacaca ggaaacagta ttcatgtccc ct
//
