LOCUS       Exported               10657 bp ds-DNA     circular SYN 04-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAK148_dCas9-10xST(22aa)
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Jeltsch Group
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 29, 2024 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        367..642
                     /label=chicken beta-actin promoter
     intron          643..1651
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             1753..1773
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      1822..1831
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1828..5931
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5935..5961
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5980..6000
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6007..6027
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6091..6147
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6214..6270
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6337..6393
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6460..6516
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6583..6639
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6706..6762
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6829..6885
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             6952..7008
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7075..7131
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             7198..7254
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     polyA_signal    7445..7566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7573..8028)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        8055..8159
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8161..8518
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8369..8504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8553..9347
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    9579..9626
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9955..10543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      421 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      481 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
      541 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      601 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgttgcct
      661 tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      721 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      781 gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg gctccgggag
      841 ggccctttgt gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
      901 cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      961 tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     1021 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     1081 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
     1141 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
     1201 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     1261 gctcggggga ggggcgcggc ggccccggag cgccggcggc tgtcgaggcg cggcgagccg
     1321 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1381 ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cgggcgaagc
     1441 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1501 gtccccttct ccatctccag cctcggggct gccgcagggg gacggctgcc ttcggggggg
     1561 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     1621 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct
     1681 gtctcatcat tttggcaaag aattctgcag tcgacggtac cgcgggcccc ctaggctacg
     1741 cgcgccacca tgcccaagaa gaagcgcaag gtgggacgcg tctgcaggat atcaagcttg
     1801 cggtaccgcg ggcccgggat cgccaccatg gacaagaagt acagcatcgg cctggccatc
     1861 ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa
     1921 ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat cggcgccctg
     1981 ctgttcgaca gcggagaaac agccgaggcc acccggctga agagaaccgc cagaagaaga
     2041 tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc
     2101 aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag
     2161 aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag
     2221 taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg
     2281 cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag
     2341 ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc
     2401 tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc
     2461 ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc
     2521 gagaagaaga atggcctgtt cggcaacctg attgccctga gcctgggcct gacccccaac
     2581 ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac
     2641 gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg
     2701 gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag
     2761 atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac
     2821 ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc
     2881 ttcgaccaga gcaagaacgg ctacgccggc tacatcgatg gcggagccag ccaggaagag
     2941 ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg
     3001 aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc
     3061 caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca
     3121 ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac
     3181 gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa
     3241 accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgccagcgc ccagagcttc
     3301 atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac
     3361 agcctgctgt acgagtactt caccgtgtac aacgagctga ccaaagtgaa atacgtgacc
     3421 gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaagccat cgtggacctg
     3481 ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa
     3541 atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg
     3601 ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa
     3661 aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg
     3721 atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg
     3781 aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg
     3841 gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga
     3901 aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc
     3961 caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc
     4021 gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg
     4081 ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag
     4141 aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg
     4201 ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg
     4261 tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac
     4321 cggctgtccg actacgatgt ggacgctatc gtgcctcaga gctttctgaa ggacgactcc
     4381 atcgataaca aagtgctgac tcggagcgac aagaaccggg gcaagagcga caacgtgccc
     4441 tccgaagagg tcgtgaagaa gatgaagaac tactggcgcc agctgctgaa tgccaagctg
     4501 attacccaga ggaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg
     4561 gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg
     4621 gcacagatcc tggactcccg gatgaacact aagtacgacg agaacgacaa actgatccgg
     4681 gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag
     4741 ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc
     4801 gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc
     4861 gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag
     4921 gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc
     4981 ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga aacaggcgag
     5041 atcgtgtggg ataagggccg ggactttgcc accgtgcgga aagtgctgtc tatgccccaa
     5101 gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg
     5161 cccaagagga acagcgacaa gctgatcgcc agaaagaagg actgggaccc taagaagtac
     5221 ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag
     5281 ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga
     5341 agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa
     5401 aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag
     5461 agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa
     5521 tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat
     5581 aatgagcaga aacagctgtt tgtggaacag cacaaacact acctggacga gatcatcgag
     5641 cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga caaggtgctg
     5701 agcgcctaca acaagcacag agacaagcct atcagagagc aggccgagaa tatcatccac
     5761 ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc
     5821 gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc
     5881 atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga cgcctatccc
     5941 tatgacgtgc ccgattatgc cagcctgggc agcggctccc ccaagaaaaa acgcaaggtg
     6001 gaagatccta agaaaaagcg gaaagtggac ggcattggta gtgggagcaa cggcagcagc
     6061 ggatccaacg gtccgactga cgccgcggaa gaggagcttc tgagcaaaaa ctatcacctc
     6121 gaaaacgagg ttgcgcgact gaagaaagga agcgggtccg gtggaagtgg ctccggatct
     6181 ggaggttctg gcagcggagg tagcggcagt ggcgaagagc tccttagtaa gaactatcat
     6241 ctggaaaatg aggtagcgcg cttaaagaaa gggtcgggaa gtggcggcag cggaagtggg
     6301 agtggaggga gcggttctgg cggttccggc agtggagagg agttgctgtc taagaactac
     6361 cacttagaaa acgaagtcgc acggctaaaa aaaggttccg gctccggcgg ctccggttct
     6421 ggaagcgggg gctcgggatc aggtggatct ggatcaggag aggaattgct ttccaaaaac
     6481 taccaccttg agaatgaggt ggccaggtta aagaagggga gcggctcggg gggtagtgga
     6541 tcggggtcgg gcgggtcagg aagcggtggt agcggatctg gggaggagct gctctcgaag
     6601 aattaccatt tggagaacga agtggcgaga ctaaagaagg gaagcggtag tggtggttca
     6661 gggtctggtt caggtggatc caacggtccg actgacgccg cggaagagga gcttctgagc
     6721 aaaaactatc acctcgaaaa cgaggttgcg cgactgaaga aaggaagcgg gtccggtgga
     6781 agtggctccg gatctggagg ttctggcagc ggaggtagcg gcagtggcga agagctcctt
     6841 agtaagaact atcatctgga aaatgaggta gcgcgcttaa agaaagggtc gggaagtggc
     6901 ggcagcggaa gtgggagtgg agggagcggt tctggcggtt ccggcagtgg agaggagttg
     6961 ctgtctaaga actaccactt agaaaacgaa gtcgcacggc taaaaaaagg ttccggctcc
     7021 ggcggctccg gttctggaag cgggggctcg ggatcaggtg gatctggatc aggagaggaa
     7081 ttgctttcca aaaactacca ccttgagaat gaggtggcca ggttaaagaa ggggagcggc
     7141 tcggggggta gtggatcggg gtcgggcggg tcaggaagcg gtggtagcgg atctggggag
     7201 gagctgctct cgaagaatta ccatttggag aacgaagtgg cgagactaaa gaagggaagc
     7261 ggtagtggtg gttcagggtc tggttcaggt ggcagtgggt ctgggggctc agggtccggg
     7321 taggcggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     7381 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     7441 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7501 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7561 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     7621 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     7681 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     7741 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     7801 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     7861 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     7921 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     7981 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     8041 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8101 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     8161 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8221 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8281 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8341 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8401 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8461 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     8521 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     8581 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     8641 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     8701 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     8761 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     8821 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     8881 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     8941 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     9001 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     9061 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     9121 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     9181 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     9241 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     9301 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     9361 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     9421 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     9481 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     9541 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     9601 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     9661 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     9721 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     9781 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     9841 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9901 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9961 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    10021 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    10081 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
    10141 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    10201 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    10261 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    10321 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    10381 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    10441 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    10501 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    10561 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    10621 ccctgattct gtggataacc gtattaccgc catgcat
//
