LOCUS       ON060706                5222 bp    DNA     circular SYN 01-AUG-2022
DEFINITION  Synthetic construct clone pBH001, complete sequence.
ACCESSION   ON060706
VERSION     ON060706.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Huang,B.D., Groseclose,T.M. and Wilson,C.J.
  TITLE     Transcriptional programming in a Bacteroides consortium
  JOURNAL   Nat Commun 13 (1), 3901 (2022)
   PUBMED   35794179
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Huang,B.D., Groseclose,T.M. and Wilson,C.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2022) Chemical & Biomolecular Engineering,
            Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA
            30332, USA
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /clone="pBH001"
                     /note="derived from Escherichia coli strain EC100D Pir116"
     misc_feature    129..132
                     /note="Part 1 overhang"
     misc_feature    134..139
                     /note="BsmBI site"
     misc_feature    152..180
                     /note="lac promoter"
     misc_feature    188..204
                     /note="lac operator"
     misc_feature    224..547
                     /note="lacZ-alpha"
     misc_feature    complement(523..542)
                     /note="pRS-marker"
     misc_feature    560..565
                     /note="BsmBI site"
     misc_feature    567..570
                     /note="Part 8 overhang"
     misc_feature    614
                     /note="mutated to G"
     misc_feature    636
                     /note="mutated to C"
     misc_feature    637..655
                     /note="BT-r1"
     misc_feature    724..2004
                     /note="NBU2 integrase"
     misc_feature    875
                     /note="mutated to T"
     misc_feature    982
                     /note="mutated to A (silent)"
     misc_feature    1379
                     /note="mutated to T"
     misc_feature    complement(1660..1662)
                     /note="mislabeled AGC"
     misc_feature    complement(1825..1827)
                     /note="mislabeled AGC"
     misc_feature    complement(1846..1848)
                     /note="mislabeled gca"
     misc_feature    complement(1894..1896)
                     /note="mislabeled as CGC"
     misc_feature    2130
                     /note="difference"
     misc_feature    2164..2176
                     /note="attN2 5' sequence"
     misc_feature    2198..2211
                     /note="attN2 3' sequence"
     misc_feature    2444..3178
                     /note="ermG"
     misc_feature    3666..4527
                     /note="beta-lactamase from pBR322"
ORIGIN      
        1 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
       61 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac
      121 cgcggtggcc cttgagacgg catcttccat ctacacttta tgcttccggc tcgtatgttg
      181 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
      241 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
      301 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      361 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      421 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
      481 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      541 cgcataggat ctgatgcatc gtctcaccga tacttgtgcc tgttctattt ccgaaccgac
      601 cgcttgtatg aatccatcaa aattcgtttt ctctatgttg gattccttgt tgctcatatt
      661 gtgatgataa tttctacaaa tatagtcatt ggtaactatc tatgaaactg tttgatactt
      721 ttatagttga ttaaacttgt tcatggcatt tgccttaata tcatccgcta tgtcaatgta
      781 gggtttcata gctttgtagt cgctgtgtcc cgtccatttc atgaccacct gtgccgggat
      841 tccgagagcc agcgcattgc agatgaatgt ccttcttcct gcatgggtac tgagcaaagc
      901 gtatttgggt gtgacttcat caatacgttc atttcccttg tagtaggttt cccgtacagg
      961 ctcgttgatt tctgccagtt cgcccagctc tttcaggtaa tcgttcatct tctggttgct
     1021 gatgacgggc agagccatgt aattctcgaa atggatgtcc ttgtatttgt ccagtatggc
     1081 tttgctgtat ttgttcagtt caatcgtcag gctgtcggca gtcttgactg tggttatttc
     1141 gatgtggtcg gacttcacat cgcttctttt cagattgcga acatccgaat accgcaaact
     1201 cgtaaagcag cagaacagga aaacatcacg cacacgttcc aggtattgct tatccttggg
     1261 tatctggtag tctttcagct tgttcagttc atcccaagtc aggaagatta cttttttcga
     1321 ggtggttttc agtttcggtt tgaacgtatc gtatgcaatg ttctgatgat gtcctttcct
     1381 gaagctccag cgcaggaacc atttgaggaa tcccatttgc ttgccgatgg tgctgtttct
     1441 catatccttg gtgtcacgca ggaagttgac gtattcgttc aatccaaact cgttgaaata
     1501 gttgaacgtt gcatcctcct tgaactcttt gaggtggttc ctcactgctg caaatttttc
     1561 ataggtggat gccgtccagt tattctggtt accgcactct tttacaaact catcgaacac
     1621 ctcccaaaag ctgacagggg cttcttccgg ctgttcttca ctggtgtctt tcattctcat
     1681 gttgaaagct tccttcaact gttgggtcgt tggcatgacc tcctgcacct caaattcctt
     1741 gaaaatattc tggatttcgg catagtattt cagcaagtcc gtattgattt cggctgcact
     1801 ttgctttagc ttgttggtac atccgttctt tacccgctgc ttatctacat cccatttggc
     1861 tacgtcaatc cggtagcccg ttgtaaactc gatacgttgg ctggcaaaga tgacacgcat
     1921 acggatgggt acgttctcta cgattggcac accgttcttt ttccggctct ccaatgcaaa
     1981 aatgatgttg cgcttgatat tcataattgg gtgcgtttga aattctacac ccaaatatac
     2041 acccaattat tgagatagca aaagacattt agaaacattt acttttactc tatattgtaa
     2101 tttacacttg attatcagtc gtttgcagtt ttatgatatt ctgtgaaagt ataagttcga
     2161 gagcctgtct ctccgcaaaa aacgctgaaa atcagcagat tgcaaaacaa acaccctgtt
     2221 ttacacccaa gaatgtaaag tcggctgttt ttgttttatt taagataata caaccactac
     2281 ataataaaag agtagcgata ttaaaagaat ccgatgagaa aagactaata tttatctatc
     2341 cattcagttt gatttttcag gactttacat cgtcctgaaa gtatttgttg gtaccggtac
     2401 cgaggacgcg taaacattta cagttgcatg tggcctattg tttttagccg ttaaatattt
     2461 tataactatt aaatagcgat acaaattgtt cgaaactaat attgtttata tcatatattc
     2521 tcgcatgttt taaagcttta ttaaattgat tttttgtaaa cagtttttcg tactctttgt
     2581 taacccattt cattacaaaa gtttcatatt tttttctctc tttaaatgcc atttttgctg
     2641 gctttctttt taatacaatt aatgtgctat ccactttagg ttttggatgg aaataatacc
     2701 taggaatttt tgctaatata gaaatatcta cctctgccat taacagcaat gctagtgatc
     2761 tgtttgtatc taataacatt ttagcaaaac catattccac tattaaataa cttattgtgg
     2821 ctgaactttc aaaaacaatt tttcgaatta tatttgtgct tatgttgtaa ggtatgctgc
     2881 caaatatttt atatggattg tggctaggaa atgtaaattt cagtatatca tcatttacta
     2941 tttgatagtt aggataattt aagagcttat tacgagttac ctcacataat ttagaatcaa
     3001 tttctatcgc cgttacaaaa ttacatctct ttaccaatcc agcagtaaaa tgacctttcc
     3061 ctgcacctat ttcaaagatg ttatcttttt catctaaact tatgcaattc attatttttt
     3121 ctatgtgata ttttgaagta ataaaatttt gactatcttt tatatttact ttgttcatta
     3181 taacctctcc ttaatttatt gcatctcttt tcgaatattt atgttttttg agaaaagaac
     3241 gtactcatgg ttcatcccga tatgcgtatc ggtctgtata tcagcaactt tctatgtgtt
     3301 tcaactacaa tagtcatcta ttctcatctt tctgagtcca ccccctgcaa agcccctctt
     3361 tacgacataa aaattcggtc ggaaaaggta tgcaaaagat gtttctctct ttaagagaaa
     3421 ctcttcggga tgcaaaaata tgaaaataac tccaattcac caaattatat agcgactttt
     3481 ttacaaaatg ctaaaatttg ttgatttccg tcaagcaatt gttgagcaaa aatgtctttt
     3541 acgataaaat gatacctcaa tatcaactgt ttagcaaaac gatatttctc ttaaagagag
     3601 aaacaccttt ttgttcacca atccccgact tttaatcccg cggccatgat tgaaaaagga
     3661 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3721 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3781 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3841 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3901 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3961 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     4021 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     4081 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     4141 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     4201 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     4261 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     4321 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     4381 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     4441 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4501 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcataa cgcgtcaatt
     4561 cgagggggat caattccgtg ataggtgggc tgcccttcct ggttggcttg gtttcatcag
     4621 ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat
     4681 tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg
     4741 gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta
     4801 cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa
     4861 tcgctataat gaccccgaag cagggttatg cagcggaaaa cggaattgat ccggccacga
     4921 tgcgtccggc gtagaggatc tgaagatcag cagttcaacc tgttgatagt acgtactaag
     4981 ctctcatgtt tcacgtacta agctctcatg tttaacgtac taagctctca tgtttaacga
     5041 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt
     5101 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag
     5161 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta
     5221 ag
//

