LOCUS       Exported                7051 bp ds-DNA     circular SYN 09-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 29, 2024 from SnapGene Viewer 5.2.5.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    129..174
                     /locus_tag="PcfxA"
                     /label=PcfxA
     misc_feature    171..174
                     /locus_tag="-33"
                     /label=-33
     misc_feature    complement(175..194)
                     /locus_tag="Otta(1)"
                     /label=Otta
                     /label=Otta(1)
     misc_feature    195..207
                     /locus_tag="PcfxA(1)"
                     /label=PcfxA
                     /label=PcfxA(1)
     misc_feature    195..202
                     /locus_tag="-7"
                     /label=-7
     misc_feature    complement(208..227)
                     /locus_tag="Otta"
                     /label=Otta
     misc_feature    270..785
                     /locus_tag="NanoLuc"
                     /label=NanoLuc
     misc_feature    829..880
                     /locus_tag="NanoLuc Terminator"
                     /label=NanoLuc Terminator
     misc_feature    941..1249
                     /locus_tag="Pbt1311"
                     /label=Pbt1311
     misc_feature    1250..1297
                     /locus_tag="lacI"
                     /label=lacI
     misc_feature    1298..1315
                     /locus_tag="lacI(1)"
                     /label=lacI
                     /label=lacI(1)
     misc_feature    1316..2332
                     /locus_tag="lacI(2)"
                     /label=lacI
                     /label=lacI(2)
     misc_feature    2339..2399
                     /locus_tag="L3S2P21 Terminator"
                     /label=L3S2P21 Terminator
     misc_feature    2553..3833
                     /locus_tag="NBU2 integrase"
                     /label=NBU2 integrase
     misc_feature    3993..4015
                     /locus_tag="attN2"
                     /label=attN2
     misc_feature    4273..5007
                     /locus_tag="ermG"
                     /label=ermG
     misc_feature    5495..6356
                     /locus_tag="beta-lactamase from pBR322"
                     /label=beta-lactamase from pBR322
ORIGIN
        1 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
       61 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac
      121 cgcggtggta caaagaaaat tcgacaaact gttatttttc tatctattta tttgaatttt
      181 aagcgcttaa aatttacctt tgtcggcaat tttaagcgct taaaattaaa taaagatatt
      241 ctcgtcaaac aaatataaat aatataaaca tggtttttac tctggaagat tttgttggcg
      301 attggcgtca gaccgcgggt tataatttgg atcaagtcct ggaacagggt ggcgtaagct
      361 ctctgttcca gaacctgggt gtgagcgtga cgccgattca gcgcatcgtt ctgtccggcg
      421 agaacggtct gaaaattgat attcatgtga tcatcccgta cgaaggcctg agcggtgacc
      481 aaatgggtca aatcgagaaa atctttaaag tcgtctaccc agttgacgat caccacttca
      541 aggttatctt gcattacggt acgctggtga ttgatggtgt gaccccgaat atgattgact
      601 atttcggccg tccgtatgaa ggcattgccg tttttgacgg taaaaagatc accgtcaccg
      661 gtaccctgtg gaatggcaat aagattattg acgagcgtct gattaacccg gacggcagcc
      721 tgctgttccg cgtgaccatc aacggtgtca cgggttggcg tctgtgcgag cgcatcctgg
      781 cataatgaac tgcacttgct ttgataatta atgataaaca atctaaaagc actctaatcg
      841 ttatcggagt gcttttagat tactaatcaa attgcttcta ctaattgcct atcttccagt
      901 gatggaacag catttgtgca ttggctgcaa caatcagcct tgatctggaa gaagcaatga
      961 aagctgctgt taagtctccg aatcaggtat tgttcctgac aggtgtattc ccatccggta
     1021 aacgcggata ctttgcagtt gatctgactc aggaataaat tataaattaa ggtaagaaga
     1081 ttgtaggata agctaatgaa atagaaaaag gatgccgtca cacaacttgt cggcattctt
     1141 ttttgtttta ttagttgaaa atatagtgaa aaagttgcct aaatatgtat gttaacaaat
     1201 tatttgtcgt aactttgcac tccaaatctg tttttaacat atggcactag tgaaaccagt
     1261 aacgttatac gatgtcgcag agtatgccgg tgtctctacc gcgaccgttt ccaacgtggt
     1321 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga
     1381 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat
     1441 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa
     1501 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt
     1561 cgaagcctgt aaaacggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat
     1621 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc
     1681 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga
     1741 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct
     1801 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata
     1861 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc
     1921 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt
     1981 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt
     2041 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc
     2101 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     2161 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     2221 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2281 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagctttcct
     2341 cggtaccaaa ttccagaaaa gaggcctccc gaaagggggg ccttttttcg ttttggtcct
     2401 acttgtgcct gttctatttc cgaaccgacc gcttgtatga atccatcaaa attcgttttc
     2461 tctatgttgg attccttgtt gctcatattg tgatgataat ttctacaaat atagtcattg
     2521 gtaactatct atgaaactgt ttgatacttt tatagttgat taaacttgtt catggcattt
     2581 gccttaatat catccgctat gtcaatgtag ggtttcatag ctttgtagtc gctgtgtccc
     2641 gtccatttca tgaccacctg tgccgggatt ccgagagcca gcgcattgca gatgaatgtc
     2701 cttcttcctg catgggtact gagcaaagcg tatttgggtg tgacttcatc aatacgttca
     2761 tttcccttgt agtaggtttc ccgtacaggc tcgttgattt ctgccagttc gcccagctct
     2821 ttcaggtaat cgttcatctt ctggttgctg atgacgggca gagccatgta attctcgaaa
     2881 tggatgtcct tgtatttgtc cagtatggct ttgctgtatt tgttcagttc aatcgtcagg
     2941 ctgtcggcag tcttgactgt ggttatttcg atgtggtcgg acttcacatc gcttcttttc
     3001 agattgcgaa catccgaata ccgcaaactc gtaaagcagc agaacaggaa aacatcacgc
     3061 acacgttcca ggtattgctt atccttgggt atctggtagt ctttcagctt gttcagttca
     3121 tcccaagtca ggaagattac ttttttcgag gtggttttca gtttcggttt gaacgtatcg
     3181 tatgcaatgt tctgatgatg tcctttcttg aagctccagc gcaggaacca tttgaggaat
     3241 cccatttgct tgccgatggt gctgtttctc atatccttgg tgtcacgcag gaagttgacg
     3301 tattcgttca atccaaactc gttgaaatag ttgaacgttg catcctcctt gaactctttg
     3361 aggtggttcc tcactgctgc aaatttttca taggtggatg ccgtccagtt attctggtta
     3421 ccgcactctt ttacaaactc atcgaacacc tcccaaaagc tgacaggggc ttcttccggc
     3481 tgttcttcgc tggtgtcttt cattctcatg ttgaaagctt ccttcaactg ttgggtcgtt
     3541 ggcatgacct cctgcacctc aaattccttg aaaatattct ggatttcggc atagtatttc
     3601 agcaagtccg tattgatttc ggctgcactt tgctttagct tgttggtaca tccgctcttt
     3661 acccgctgct tatctgcatc ccatttggct acgtcaatcc ggtagcccgt tgtaaactcg
     3721 atgcgttggc tggcaaagat gacacgcata cggatgggta cgttctctac gattggcaca
     3781 ccgttctttt tccggctctc caatgcaaaa atgatgttgc gcttgatatt cataattggg
     3841 tgcgtttgaa attctacacc caaatataca cccaattatt gagatagcaa aagacattta
     3901 gaaacattta cttttactct atattgtaat ttacacttga ttatcagtcg tttgcagtct
     3961 tatgatattc tgtgaaagta taagttcgag agcctgtctc tccgcaaaaa acgctgaaaa
     4021 tcagcagatt gcaaaacaaa caccctgttt tacacccaag aatgtaaagt cggctgtttt
     4081 tgttttattt aagataatac aaccactaca taataaaaga gtagcgatat taaaagaatc
     4141 cgatgagaaa agactaatat ttatctatcc attcagtttg atttttcagg actttacatc
     4201 gtcctgaaag tatttgttgg taccggtacc gaggacgcgt aaacatttac agttgcatgt
     4261 ggcctattgt ttttagccgt taaatatttt ataactatta aatagcgata caaattgttc
     4321 gaaactaata ttgtttatat catatattct cgcatgtttt aaagctttat taaattgatt
     4381 ttttgtaaac agtttttcgt actctttgtt aacccatttc attacaaaag tttcatattt
     4441 ttttctctct ttaaatgcca tttttgctgg ctttcttttt aatacaatta atgtgctatc
     4501 cactttaggt tttggatgga aataatacct aggaattttt gctaatatag aaatatctac
     4561 ctctgccatt aacagcaatg ctagtgatct gtttgtatct aataacattt tagcaaaacc
     4621 atattccact attaaataac ttattgtggc tgaactttca aaaacaattt ttcgaattat
     4681 atttgtgctt atgttgtaag gtatgctgcc aaatatttta tatggattgt ggctaggaaa
     4741 tgtaaatttc agtatatcat catttactat ttgatagtta ggataattta agagcttatt
     4801 acgagttacc tcacataatt tagaatcaat ttctatcgcc gttacaaaat tacatctctt
     4861 taccaatcca gcagtaaaat gacctttccc tgcacctatt tcaaagatgt tatctttttc
     4921 atctaaactt atgcaattca ttattttttc tatgtgatat tttgaagtaa taaaattttg
     4981 actatctttt atatttactt tgttcattat aacctctcct taatttattg catctctttt
     5041 cgaatattta tgttttttga gaaaagaacg tactcatggt tcatcccgat atgcgtatcg
     5101 gtctgtatat cagcaacttt ctatgtgttt caactacaat agtcatctat tctcatcttt
     5161 ctgagtccac cccctgcaaa gcccctcttt acgacataaa aattcggtcg gaaaaggtat
     5221 gcaaaagatg tttctctctt taagagaaac tcttcgggat gcaaaaatat gaaaataact
     5281 ccaattcacc aaattatata gcgacttttt tacaaaatgc taaaatttgt tgatttccgt
     5341 caagcaattg ttgagcaaaa atgtctttta cgataaaatg atacctcaat atcaactgtt
     5401 tagcaaaacg atatttctct taaagagaga aacacctttt tgttcaccaa tccccgactt
     5461 ttaatcccgc ggccatgatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5521 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5581 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5641 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5701 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5761 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5821 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5881 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5941 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6001 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6061 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6121 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6181 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6241 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6301 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6361 agaccaagtt tactcataac gcgtcaattc gagggggatc aattccgtga taggtgggct
     6421 gcccttcctg gttggcttgg tttcatcagc catccgcttg ccctcatctg ttacgccggc
     6481 ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
     6541 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
     6601 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
     6661 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
     6721 agcggaaaac ggaattgatc cggccacgat gcgtccggcg tagaggatct gaagatcagc
     6781 agttcaacct gttgatagta cgtactaagc tctcatgttt cacgtactaa gctctcatgt
     6841 ttaacgtact aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc
     6901 tcatggctaa cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata
     6961 aaattaatat aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa
     7021 aagaatatat aaggctttta aagcttttaa g
//
