LOCUS       Exported                7085 bp ds-DNA     circular SYN 09-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 29, 2024 from SnapGene Viewer 5.2.5.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    129..207
                     /locus_tag="PcfxA"
                     /label=PcfxA
     misc_feature    171..174
                     /locus_tag="-33"
                     /label=-33
     misc_feature    175..195
                     /locus_tag="O1(1)"
                     /label=O1
                     /label=O1(1)
     misc_feature    196..202
                     /locus_tag="-7"
                     /label=-7
     misc_feature    208..228
                     /locus_tag="O1"
                     /label=O1
     misc_feature    271..786
                     /locus_tag="NanoLuc"
                     /label=NanoLuc
     misc_feature    830..881
                     /locus_tag="NanoLuc Terminator"
                     /label=NanoLuc Terminator
     misc_feature    942..1250
                     /locus_tag="Pbt1311"
                     /label=Pbt1311
     misc_feature    1251..1316
                     /locus_tag="lacI"
                     /label=lacI
     misc_feature    1299..1301
                     /locus_tag="17"
                     /label=17
     misc_feature    1302..1304
                     /locus_tag="18"
                     /label=18
     misc_feature    1314..1316
                     /locus_tag="22"
                     /label=22
     misc_feature    1317..1350
                     /locus_tag="lacI(1)"
                     /label=lacI
                     /label=lacI(1)
     misc_feature    1351..2249
                     /locus_tag="RbsR"
                     /label=RbsR
     misc_feature    2373..2433
                     /locus_tag="L3S2P21 Terminator"
                     /label=L3S2P21 Terminator
     misc_feature    2587..3867
                     /locus_tag="NBU2 integrase"
                     /label=NBU2 integrase
     misc_feature    4027..4049
                     /locus_tag="attN2"
                     /label=attN2
     misc_feature    4307..5041
                     /locus_tag="ermG"
                     /label=ermG
     misc_feature    5529..6390
                     /locus_tag="beta-lactamase from pBR322"
                     /label=beta-lactamase from pBR322
ORIGIN
        1 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
       61 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac
      121 cgcggtggta caaagaaaat tcgacaaact gttatttttc tatctattta tttgaattgt
      181 gagcggataa caattacctt tgtcggcaat tgtgagcgga taacaattaa ataaagatat
      241 tctcgtcaaa caaatataaa taatataaac atggttttta ctctggaaga ttttgttggc
      301 gattggcgtc agaccgcggg ttataatttg gatcaagtcc tggaacaggg tggcgtaagc
      361 tctctgttcc agaacctggg tgtgagcgtg acgccgattc agcgcatcgt tctgtccggc
      421 gagaacggtc tgaaaattga tattcatgtg atcatcccgt acgaaggcct gagcggtgac
      481 caaatgggtc aaatcgagaa aatctttaaa gtcgtctacc cagttgacga tcaccacttc
      541 aaggttatct tgcattacgg tacgctggtg attgatggtg tgaccccgaa tatgattgac
      601 tatttcggcc gtccgtatga aggcattgcc gtttttgacg gtaaaaagat caccgtcacc
      661 ggtaccctgt ggaatggcaa taagattatt gacgagcgtc tgattaaccc ggacggcagc
      721 ctgctgttcc gcgtgaccat caacggtgtc acgggttggc gtctgtgcga gcgcatcctg
      781 gcataatgaa ctgcacttgc tttgataatt aatgataaac aatctaaaag cactctaatc
      841 gttatcggag tgcttttaga ttactaatca aattgcttct actaattgcc tatcttccag
      901 tgatggaaca gcatttgtgc attggctgca acaatcagcc ttgatctgga agaagcaatg
      961 aaagctgctg ttaagtctcc gaatcaggta ttgttcctga caggtgtatt cccatccggt
     1021 aaacgcggat actttgcagt tgatctgact caggaataaa ttataaatta aggtaagaag
     1081 attgtaggat aagctaatga aatagaaaaa ggatgccgtc acacaacttg tcggcattct
     1141 tttttgtttt attagttgaa aatatagtga aaaagttgcc taaatatgta tgttaacaaa
     1201 ttatttgtcg taactttgca ctccaaatct gtttttaaca tatggcacta gtgaaaccag
     1261 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     1321 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     1381 agctcaatta cattcccaac cgcgtggcac aacaactggc gggcaaagcg tcgcatacca
     1441 ttggcatgtt gatcactgcc agtaccaatc ctttctattc agaactggtg cgtggcgttg
     1501 aacgcagctg cttcgaacgc ggttatagtc tcgatctttg caataccgaa ggcgatgaac
     1561 agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggc ttgctgttac
     1621 tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg acagtgccta
     1681 ctgtgatgat ggactgggct ccgttcgatg gcgacagcga tcttattcag gataactcgt
     1741 tgctgggcgg agacttagca acgcaatatc tgatcgataa aggtcatacc cgtatcgcct
     1801 gtattaccgg cccgctggat aaaactccgg cgcgcctgcg gttggaaggt tatcgggcgg
     1861 cgatgaaacg tgcgggtctc aacattcctg atggctatga agtcactggt gattttgaat
     1921 ttaacggcgg gtttgacgct atgcgccaac tgctatcaca tccgctgcgt cctcaggccg
     1981 tctttaccgg aaatgacgct atggctgttg gcgtttacca ggcgttatat caggcagagt
     2041 tacaggttcc gcaggatatc gcggtgattg gctatgacga tatcgaactg gcaagcttta
     2101 tgacgccacc attaaccact atccaccaac cgaaagatga actgggggag ctggcgattg
     2161 atgtactcat ccatcggata acccagccga cccttcagca acaacgatta caacttactc
     2221 cgattctgat ggaacgcggt tcggcttagc tggtgaaaag aaaaaccacc ctggcgccca
     2281 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     2341 tttcccgact ggaaagcggg cagtgagctt tcctcggtac caaattccag aaaagaggcc
     2401 tcccgaaagg ggggcctttt ttcgttttgg tcctacttgt gcctgttcta tttccgaacc
     2461 gaccgcttgt atgaatccat caaaattcgt tttctctatg ttggattcct tgttgctcat
     2521 attgtgatga taatttctac aaatatagtc attggtaact atctatgaaa ctgtttgata
     2581 cttttatagt tgattaaact tgttcatggc atttgcctta atatcatccg ctatgtcaat
     2641 gtagggtttc atagctttgt agtcgctgtg tcccgtccat ttcatgacca cctgtgccgg
     2701 gattccgaga gccagcgcat tgcagatgaa tgtccttctt cctgcatggg tactgagcaa
     2761 agcgtatttg ggtgtgactt catcaatacg ttcatttccc ttgtagtagg tttcccgtac
     2821 aggctcgttg atttctgcca gttcgcccag ctctttcagg taatcgttca tcttctggtt
     2881 gctgatgacg ggcagagcca tgtaattctc gaaatggatg tccttgtatt tgtccagtat
     2941 ggctttgctg tatttgttca gttcaatcgt caggctgtcg gcagtcttga ctgtggttat
     3001 ttcgatgtgg tcggacttca catcgcttct tttcagattg cgaacatccg aataccgcaa
     3061 actcgtaaag cagcagaaca ggaaaacatc acgcacacgt tccaggtatt gcttatcctt
     3121 gggtatctgg tagtctttca gcttgttcag ttcatcccaa gtcaggaaga ttactttttt
     3181 cgaggtggtt ttcagtttcg gtttgaacgt atcgtatgca atgttctgat gatgtccttt
     3241 cttgaagctc cagcgcagga accatttgag gaatcccatt tgcttgccga tggtgctgtt
     3301 tctcatatcc ttggtgtcac gcaggaagtt gacgtattcg ttcaatccaa actcgttgaa
     3361 atagttgaac gttgcatcct ccttgaactc tttgaggtgg ttcctcactg ctgcaaattt
     3421 ttcataggtg gatgccgtcc agttattctg gttaccgcac tcttttacaa actcatcgaa
     3481 cacctcccaa aagctgacag gggcttcttc cggctgttct tcgctggtgt ctttcattct
     3541 catgttgaaa gcttccttca actgttgggt cgttggcatg acctcctgca cctcaaattc
     3601 cttgaaaata ttctggattt cggcatagta tttcagcaag tccgtattga tttcggctgc
     3661 actttgcttt agcttgttgg tacatccgct ctttacccgc tgcttatctg catcccattt
     3721 ggctacgtca atccggtagc ccgttgtaaa ctcgatgcgt tggctggcaa agatgacacg
     3781 catacggatg ggtacgttct ctacgattgg cacaccgttc tttttccggc tctccaatgc
     3841 aaaaatgatg ttgcgcttga tattcataat tgggtgcgtt tgaaattcta cacccaaata
     3901 tacacccaat tattgagata gcaaaagaca tttagaaaca tttactttta ctctatattg
     3961 taatttacac ttgattatca gtcgtttgca gtcttatgat attctgtgaa agtataagtt
     4021 cgagagcctg tctctccgca aaaaacgctg aaaatcagca gattgcaaaa caaacaccct
     4081 gttttacacc caagaatgta aagtcggctg tttttgtttt atttaagata atacaaccac
     4141 tacataataa aagagtagcg atattaaaag aatccgatga gaaaagacta atatttatct
     4201 atccattcag tttgattttt caggacttta catcgtcctg aaagtatttg ttggtaccgg
     4261 taccgaggac gcgtaaacat ttacagttgc atgtggccta ttgtttttag ccgttaaata
     4321 ttttataact attaaatagc gatacaaatt gttcgaaact aatattgttt atatcatata
     4381 ttctcgcatg ttttaaagct ttattaaatt gattttttgt aaacagtttt tcgtactctt
     4441 tgttaaccca tttcattaca aaagtttcat atttttttct ctctttaaat gccatttttg
     4501 ctggctttct ttttaataca attaatgtgc tatccacttt aggttttgga tggaaataat
     4561 acctaggaat ttttgctaat atagaaatat ctacctctgc cattaacagc aatgctagtg
     4621 atctgtttgt atctaataac attttagcaa aaccatattc cactattaaa taacttattg
     4681 tggctgaact ttcaaaaaca atttttcgaa ttatatttgt gcttatgttg taaggtatgc
     4741 tgccaaatat tttatatgga ttgtggctag gaaatgtaaa tttcagtata tcatcattta
     4801 ctatttgata gttaggataa tttaagagct tattacgagt tacctcacat aatttagaat
     4861 caatttctat cgccgttaca aaattacatc tctttaccaa tccagcagta aaatgacctt
     4921 tccctgcacc tatttcaaag atgttatctt tttcatctaa acttatgcaa ttcattattt
     4981 tttctatgtg atattttgaa gtaataaaat tttgactatc ttttatattt actttgttca
     5041 ttataacctc tccttaattt attgcatctc ttttcgaata tttatgtttt ttgagaaaag
     5101 aacgtactca tggttcatcc cgatatgcgt atcggtctgt atatcagcaa ctttctatgt
     5161 gtttcaacta caatagtcat ctattctcat ctttctgagt ccaccccctg caaagcccct
     5221 ctttacgaca taaaaattcg gtcggaaaag gtatgcaaaa gatgtttctc tctttaagag
     5281 aaactcttcg ggatgcaaaa atatgaaaat aactccaatt caccaaatta tatagcgact
     5341 tttttacaaa atgctaaaat ttgttgattt ccgtcaagca attgttgagc aaaaatgtct
     5401 tttacgataa aatgatacct caatatcaac tgtttagcaa aacgatattt ctcttaaaga
     5461 gagaaacacc tttttgttca ccaatccccg acttttaatc ccgcggccat gattgaaaaa
     5521 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5581 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5641 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5701 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5761 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5821 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5881 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5941 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6001 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6061 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6121 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6181 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6241 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6301 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6361 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca taacgcgtca
     6421 attcgagggg gatcaattcc gtgataggtg ggctgccctt cctggttggc ttggtttcat
     6481 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
     6541 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
     6601 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
     6661 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
     6721 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaacggaatt gatccggcca
     6781 cgatgcgtcc ggcgtagagg atctgaagat cagcagttca acctgttgat agtacgtact
     6841 aagctctcat gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa
     6901 cgaactaaac cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat
     6961 gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa
     7021 tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt
     7081 ttaag
//
