LOCUS       Exported               11197 bp ds-DNA     circular SYN 09-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 29, 2024 from SnapGene Viewer 5.2.5.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_recomb     1..100
                     /locus_tag="RP4 oriT/RSK oriV"
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     misc_feature    115..118
                     /locus_tag="scar 1"
                     /label=scar 1
     misc_feature    complement(139..191)
                     /locus_tag="ECK120033736 terminator"
                     /label=ECK120033736 terminator
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    209..212
                     /locus_tag="scar 3"
                     /label=scar 3
     misc_feature    213..313
                     /locus_tag="New Feature(2)"
                     /label=New Feature
                     /label=New Feature(2)
     regulatory      327..383
                     /regulatory_class="terminator"
                     /locus_tag="L3S2P55"
                     /label=L3S2P55
                     /note="L3S2P55"
     misc_feature    345
                     /locus_tag="New Feature(1)"
                     /label=New Feature
                     /label=New Feature(1)
     regulatory      384..427
                     /regulatory_class="promoter"
                     /locus_tag="P1"
                     /label=P1
                     /note="P1"
     regulatory      391..394
                     /regulatory_class="other"
                     /locus_tag="-33 sigma factor recognitions site"
                     /label=-33 sigma factor recognitions site
                     /note="-33 sigma factor recognitions site"
     regulatory      416..423
                     /regulatory_class="other"
                     /locus_tag="-7 sigma factor recognitions site"
                     /label=-7 sigma factor recognitions site
                     /note="-7 sigma factor recognitions site"
     CDS             460..4566
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="Cas9"
                     /product="Cas9"
                     /label=Cas9
                     /protein_id="QJT41641.1"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGEFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    651
                     /locus_tag="mut to T for domestication(1)"
                     /label=mut to T for domestication
                     /label=mut to T for domestication(1)
     misc_feature    2977..2978
                     /locus_tag="H840A"
                     /label=H840A
                     /note="H840A"
     misc_difference 3770
                     /locus_tag="Gly to Glu"
                     /replace="g"
                     /label=Gly to Glu
                     /note="Gly to Glu"
     misc_feature    complement(4567..4575)
                     /locus_tag="5' PcfxA"
                     /label=5' PcfxA
     misc_feature    4577..4580
                     /locus_tag="Part 8 overhang"
                     /label=Part 8 overhang
     misc_feature    4683
                     /locus_tag="New Feature"
                     /label=New Feature
     misc_feature    4705
                     /locus_tag="New Feature(3)"
                     /label=New Feature
                     /label=New Feature(3)
     CDS             complement(4793..6130)
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="IntN1"
                     /product="IntN1"
                     /label=IntN1
                     /note="NBU1 integrase"
                     /protein_id="QJT41633.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNFRVVKRALLRYELYVRATKRGQK
                     GFILDVDLVTPDTLRDMWDFFQNEYQYYELYPSIYEAIPEKRTPQPRSKNTLIDCFSRI
                     RTFFLWCFDNKRTTNRPFDKFPIEECTYGTPYYITLEERDRIFNADLSATPQLAIQRDI
                     FIFQTLIGCRVSDLYRMTKLNVVNEAIEYIPKKTKEGNPVTVRVPLNDKAKEILERYKE
                     YEGKLLPFISEQKYNDAIKKIFKLAGVDRIVTILDPLTHNEIKRPIYEVASSHLARRTF
                     IGNIYKKVKDPNLVSALSGHKEGSKAFRRYRDIDEEMKKDLVKLLD"
     misc_feature    4799
                     /locus_tag="mut to C"
                     /label=mut to C
     misc_feature    4801
                     /locus_tag="mut to G"
                     /label=mut to G
     misc_feature    5004
                     /locus_tag="mut to T (silent)"
                     /label=mut to T (silent)
     misc_recomb     complement(6146..6475)
                     /locus_tag="attN1 region"
                     /label=attN1 region
                     /note="attN1 region"
     misc_feature    complement(6335..6348)
                     /locus_tag="CC Region"
                     /label=CC Region
                     /note="CC Region"
     misc_difference complement(6336)
                     /locus_tag="mutated for higher integration efficiency"
                     /replace="g"
                     /label=mutated for higher integration efficiency
                     /note="mutated for higher integration efficiency"
     misc_feature    6558..6577
                     /locus_tag="NBU1-intSeqRev"
                     /label=NBU1-intSeqRev
     misc_feature    6929..6930
                     /locus_tag="deleted?"
                     /label=deleted?
     CDS             7197..9122
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="TetQ"
                     /product="TetQ"
                     /label=TetQ
                     /protein_id="QJT41639.1"
                     /translation="MNIINLGILAHIDAGKTSVTENLLFASGATEKCGCVDNGDTITDS
                     MDIEKRRGITVRASTTSIIWNGVKCNIIDTPGHMDFIAEVERTFKMLDGAVLILSAKEG
                     IQAQTKLLFNTLQKLQIPTIIFINKIDRAGVNLERLYLDIKANLSQDVLFMQNVVDGSV
                     YPVCSQTYIKEEYKEFVCNHDDNILERYLADSEISPADYWNTIIALVAKAKVYPVLHGS
                     AMFNIGINELLDAITSFILPPASVSNRLSSYLYKIEHDPKGHKRSFLKIIDGSLRLRDV
                     VRINDSEKFIKIKNLKTINQGREINVDEVGANDIAIVEDMDDFRIGNYLGAEPCLIQGL
                     SHQHPALKSSVRPDRPEERSKVISALNTLWIEDPSLSFSINSYSDELEISLYGLTQKEI
                     IQTLLEERFSVKVHFDEIKTIYKERPVKKVNKIIQIEVPPNPYWATIGLTLEPLPLGTG
                     LQIESDISYGYLNHSFQNAVFEGIRMSCQSGLHGWEVTDLKVTFTQAEYYSPVSTPADF
                     RQLTPYVFRLALQQSGVDILEPMLYFELQIPQAASSKAITDLQKMMSEIEDISCNNEWC
                     HIKGKVPLNTSKDYASEVSSYTKGLGIFMVKPCGYQITKGGYSDNIRMNEKDKLLFMFQ
                     KSMSSK"
     misc_feature    8033
                     /locus_tag="mut to T for domestication"
                     /label=mut to T for domestication
     CDS             9613..10473
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="beta-lactamase"
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="derived from pBR322"
                     /protein_id="QJT41632.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_difference 10332
                     /locus_tag="misc_difference"
                     /replace="t"
                     /label=misc_difference
     misc_recomb     10512..11197
                     /locus_tag="RP4 oriT/RSK oriV(1)"
                     /label=RP4 oriT/RSK oriV
                     /label=RP4 oriT/RSK oriV(1)
                     /note="RP4 oriT/RSK oriV"
ORIGIN
        1 gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac
       61 acttaacggc tgacatggga attcccctcc accgcggtgt attaatgcgg ctgcccctgc
      121 acctgggtag ctcaatgcgc gcaataaaaa agcccccgga aggtgatctt ccgggggctt
      181 tctcatgcgt tggtgttcac tcccattcta tgcggttcct ggccttttgc tggccttttg
      241 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      301 agtgagctga tacatcccaa atgctgctcg gtaccaaaga cgaacaataa gacgctgaaa
      361 agcgtctttt ttcgttttgg tccgataaag tttggaagat aaagctaaaa gttcttatct
      421 ttgcagtccg aaataaagac atataaaaga aaagacacca tggataagaa atactcaata
      481 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
      541 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
      601 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
      661 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
      721 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
      781 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
      841 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
      901 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
      961 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     1021 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     1081 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     1141 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     1201 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     1261 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     1321 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     1381 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     1441 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     1501 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     1561 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     1621 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     1681 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     1741 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     1801 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     1861 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     1921 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     1981 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     2041 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     2101 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     2161 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     2221 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     2281 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     2341 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     2401 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     2461 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     2521 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     2581 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     2641 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     2701 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     2761 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     2821 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     2881 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     2941 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt
     3001 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     3061 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     3121 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggtggt
     3181 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     3241 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     3301 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     3361 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     3421 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     3481 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     3541 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     3601 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     3661 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     3721 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcga attctccaag
     3781 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     3841 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     3901 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     3961 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     4021 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     4081 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     4141 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     4201 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     4261 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     4321 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     4381 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     4441 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     4501 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     4561 gactgattct ttgtacccga taattgccta tcttccagtg atggaacagc atttgtgcat
     4621 tggctgcaac aatcagcctt acttgtgcct gttctatttc cgaaccgacc gcttgtatga
     4681 atgcatcaaa attcgttttc tctacgttgg attccttgtt gctcatattg tgatgataat
     4741 ttctacaaat atagtcattg gtaactatct atgaaactgt ttgatacttt tatcagtcta
     4801 atagttttac aaggtctttc ttcatttctt cgtcaatatc cctgtatcgt ctgaaagctt
     4861 tgcttccctc cttgtgtccc gacagtgcgg aaacaaggtt cgggtctttc acttttttat
     4921 agatattgcc gataaacgta cgtcttgcca gatggctgct tgccacttca taaataggtc
     4981 gtttgatttc gttgtgcgtc aacgggtcta agattgttac gatgcggtca actccagcta
     5041 atttgaatat cttttttatg gcatcattgt acttttgctc ggatatgaac ggcaacagtt
     5101 ttccctcata ttctttgtag cgttcaagga tttctttcgc tttgtcgtta agtggaacac
     5161 gtaccgtaac cggattcccc tctttggttt tcttgggaat atattctatg gcttcattga
     5221 ccacatttag tttggtcatt cggtacaggt cgctcaccct gcatcctatc agtgtctgaa
     5281 atatgaatat atccctctgt attgccagtt gtggggtggc agaaaggtct gcattaaaaa
     5341 tcctgtccct ttcttcgagt gttatataat aaggtgtacc atatgtacac tcctctatcg
     5401 gaaacttgtc gaaaggtctg tttgtggtgc gtttgttatc gaagcaccac aggaagaatg
     5461 tgcgtattct tgaaaaacag tctatcagcg tgtttttgct tctgggctgt ggtgtcctct
     5521 tttcgggaat ggcttcataa atgctcgggt aaagttcata atactggtat tcgttctgaa
     5581 agaaatccca catatcccga agcgtgtcag gtgttaccaa atccacatca aggataaagc
     5641 ccttttgtcc tctctttgta gcccttacat atagttcata acgcagtaag gctcttttga
     5701 caacccggaa atttttcttt cgtacttccg acaaagggtg cttgtttaga aattcatcga
     5761 atagttctcc aatggtaggc ttgataacca cttcctccgg caaaaaatat ttttcaggat
     5821 ggtaaaattt atcaagtgtt gtttttagcc attctttgtc tattgcttcc ttttcttgtt
     5881 gatatacctt ctcgatatag gttttcaact ggcgtatctc ttcatttata tgggtacgca
     5941 tttcttcatt gcagacagct ttcgttttta cacattcgtc tttatcatcc catagattgg
     6001 gattgatggc taactgagta ggagcaacgg agtctaactg ccttccgttt ctaaaacgga
     6061 catagattgt agccatggat tctgtatcat atcgtttggc tgctttttta atgataaagg
     6121 ttactttcat agactttcag gttgaatttt actctgctgc aaatataaat attttcccca
     6181 gcattttccc cacatctgct aaatattttg caattcgatt aaacttggat taaaatttaa
     6241 taggattata acatattgaa atacagtgta attgtggcgt ttttccgcat ttttcttttt
     6301 acccacattt tccccacttt aattatttca aatgcggggt ctgggtacaa gaaagaaagc
     6361 taagtatttg atagttcaat acttagcttt ttcttttgct tgaattttcc ccacattttc
     6421 cccacacgtg caaaaaatat agcagtaagt cattatttct tttggttgaa cgtagagagt
     6481 agcgatatta aaagaatccg atgagaaaag actaatattt atctatccat tcagtttgat
     6541 ttttcaggac tttacatcgt cctgaaagta tttgttggta ccggtaccga ggacgcgtaa
     6601 acatttacag ttgcatgtgg cctattgttt aggacgcgtt atctccttaa cgtacgtttt
     6661 cgttccattg gccctcaaac cccgttatat acattcatgt ccatttatgt aaaaaatcct
     6721 gctgaccttg tttatgtctt gtcagtcacc atttgcaaaa ccatatttga ccctcaaaga
     6781 ggctgaattt gataagcaac ttgctacata ctcataataa ggagctaaat agaacacgaa
     6841 tgggaaatac tcaaatgcca aactaaagaa gatattggcc aaaataaacg ctataccgag
     6901 agagaaactt gatttttcaa cttcctaacc aacagtgttg ttcaaacatt tctacttatt
     6961 tgtacttacc agttgaacct acgtttccct aataaaatgt ctatggtaaa aagttaaaaa
     7021 atcctcctac ttttgttaga tatatttttt tgtgtaattt tgtaatcgtt atgcggcagt
     7081 aataatatac atattaatac gagttaggaa tcctgtagtt ctcatatgct acgaggaggt
     7141 attaaaaggt gcgtttcgac aatgcatcta ttgtagtata ttattgctta atccaaatga
     7201 atattataaa tttaggaatt cttgctcaca ttgatgcagg aaaaacttcc gtaaccgaga
     7261 atctgctgtt tgccagtgga gcaacggaaa agtgcggctg tgtggataat ggtgacacca
     7321 taacggactc tatggatata gagaaacgta gaggaattac tgttcgggct tctacgacat
     7381 ctattatctg gaatggtgtg aaatgcaata tcattgacac tccgggacac atggatttta
     7441 ttgcggaagt ggagcggaca ttcaaaatgc ttgatggagc agtcctcatc ttatccgcaa
     7501 aggaaggcat acaagcgcag acaaagttgc tgttcaatac tttacagaag ctgcaaatcc
     7561 cgacaattat atttatcaat aagattgacc gagccggtgt gaatttggag cgtttgtatc
     7621 tggatataaa agcaaatctg tctcaagatg tcctgtttat gcaaaatgtt gtcgatggat
     7681 cggtttatcc ggtttgctcc caaacatata taaaggaaga atacaaagaa tttgtatgca
     7741 accatgacga caatatatta gaacgatatt tggcggatag cgaaatttca ccggctgatt
     7801 attggaatac gataatcgct cttgtggcaa aagccaaagt ctatccggtg ctacatggat
     7861 cagcaatgtt caatatcggt atcaatgagt tgttggacgc catcacttct tttatacttc
     7921 ctccggcatc ggtctcaaac agactttcat cttatcttta taagatagag catgacccca
     7981 aaggacataa aagaagtttt ctaaaaataa ttgacggaag tctgagactt cgagacgttg
     8041 taagaatcaa cgattcggaa aaattcatca agattaaaaa tctaaaaact atcaatcagg
     8101 gcagagagat aaatgttgat gaagtgggcg ccaatgatat cgcgattgta gaggatatgg
     8161 atgattttcg aatcggaaat tatttaggtg ctgaaccttg tttgattcaa ggattatcgc
     8221 atcagcatcc cgctctcaaa tcctccgtcc ggccagacag gcccgaagag agaagcaagg
     8281 tgatatccgc tctgaataca ttgtggattg aagacccgtc tttgtccttt tccataaact
     8341 catatagtga tgaattggaa atctcgttat atggtttaac ccaaaaggaa atcatacaga
     8401 cattgctgga agaacgattt tccgtaaagg tccattttga tgagatcaag actatataca
     8461 aagaacgacc tgtaaaaaag gtcaataaga ttattcagat cgaagtgccg cccaaccctt
     8521 attgggccac aatagggctg actcttgaac ccttaccgtt agggacaggg ttgcaaatcg
     8581 aaagtgacat ctcctatggt tatctgaacc attcttttca aaatgccgtt tttgaaggga
     8641 ttcgtatgtc ttgccaatcc gggttacatg gatgggaagt gactgatctg aaagtaactt
     8701 ttactcaagc cgagtattat agcccggtaa gtacacctgc tgatttcaga cagctgaccc
     8761 cttatgtctt caggctggcc ttgcaacagt caggtgtgga cattctcgaa ccgatgctct
     8821 attttgagtt gcagataccc caagcggcaa gttccaaagc tattacagat ttgcaaaaaa
     8881 tgatgtctga gattgaagac atcagttgca ataatgagtg gtgtcatatt aaagggaaag
     8941 ttccattaaa tacaagtaaa gactatgcat cagaagtaag ttcatacact aagggcttag
     9001 gcatttttat ggttaagcca tgcgggtatc aaataacaaa aggcggttat tctgataata
     9061 tccgcatgaa cgaaaaagat aaacttttat tcatgttcca aaaatcaatg tcatcaaaat
     9121 aatggtataa cctctcctta atttattgca tctcttttcg aatatttatg ttttttgaga
     9181 aaagaacgta ctcatggttc atcccgatat gcgtatcggt ctgtatatca gcaactttct
     9241 atgtgtttca actacaatag tcatctattc tcatctttct gagtccaccc cctgcaaagc
     9301 ccctctttac gacataaaaa ttcggtcgga aaaggtatgc aaaagatgtt tctctcttta
     9361 agagaaactc ttcgggatgc aaaaatatga aaataactcc aattcaccaa attatatagc
     9421 gactttttta caaaatgcta aaatttgttg atttccgtca agcaattgtt gagcaaaaat
     9481 gtcttttacg ataaaatgat acctcaatat caactgttta gcaaaacgat atttctctta
     9541 aagagagaaa cacctttttg ttcaccaatc cccgactttt aatcccgcgg ccatgattga
     9601 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     9661 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     9721 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     9781 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     9841 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     9901 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     9961 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10021 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10081 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10141 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10201 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10261 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10321 gagcgtgggt cacgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10381 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10441 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcataacgc
    10501 gtcaattcga gggggatcaa ttccgtgata ggtgggctgc ccttcctggt tggcttggtt
    10561 tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga
    10621 gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg
    10681 ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga
    10741 aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc
    10801 gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaacgg aattgatccg
    10861 gccacgatgc gtccggcgta gaggatctga agatcagcag ttcaacctgt tgatagtacg
    10921 tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt
    10981 ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc
    11041 tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact
    11101 taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa
    11161 gcttttaagg tttaacggtt gtggacaaca agccagg
//
