LOCUS       Exported                7579 bp ds-DNA     circular SYN 27-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 29, 2024 from SnapGene Viewer 5.2.5.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    129..132
                     /locus_tag="Part 1 overhang"
                     /label=Part 1 overhang
     misc_feature    134..175
                     /locus_tag="5' PcfxA"
                     /label=5' PcfxA
     misc_feature    176..179
                     /locus_tag="-33"
                     /label=-33
     misc_feature    180..199
                     /locus_tag="O1"
                     /label=O1
     misc_feature    200..207
                     /locus_tag="-7"
                     /label=-7
     misc_feature    213..233
                     /locus_tag="O1(1)"
                     /label=O1
                     /label=O1(1)
     misc_feature    238..243
                     /locus_tag="6 bp complement"
                     /label=6 bp complement
     misc_feature    244..280
                     /locus_tag="HH-Ribozyme"
                     /label=HH-Ribozyme
     misc_feature    281..300
                     /locus_tag="NL4"
                     /label=NL4
     misc_feature    301..382
                     /locus_tag="sgRNA"
                     /label=sgRNA
     misc_feature    381..382
                     /locus_tag="added for consistency"
                     /label=added for consistency
     misc_feature    383..450
                     /locus_tag="HDV Ribozyme"
                     /label=HDV Ribozyme
     misc_feature    451..502
                     /locus_tag="NanoLuc Terminator"
                     /label=NanoLuc Terminator
     misc_feature    565..873
                     /locus_tag="Pbt1311"
                     /label=Pbt1311
     misc_feature    812..815
                     /locus_tag="-33 site"
                     /label=-33 site
     misc_feature    834..841
                     /locus_tag="-7 site"
                     /label=-7 site
     misc_feature    874..1956
                     /locus_tag="lacI"
                     /label=lacI
     misc_feature    922..924
                     /locus_tag="17"
                     /label=17
     misc_feature    925..927
                     /locus_tag="18"
                     /label=18
     misc_feature    937..939
                     /locus_tag="22"
                     /label=22
     misc_feature    1963..2023
                     /locus_tag="L3S2P21 Terminator"
                     /label=L3S2P21 Terminator
     misc_feature    2050..2150
                     /locus_tag="New Feature"
                     /label=New Feature
     misc_feature    2151..2154
                     /locus_tag="BsmBI left"
                     /label=BsmBI left
     misc_feature    complement(2191..2242)
                     /locus_tag="NanoLuc Terminator(1)"
                     /label=NanoLuc Terminator
                     /label=NanoLuc Terminator(1)
     misc_feature    complement(2286..2801)
                     /locus_tag="NanoLuc"
                     /label=NanoLuc
     misc_feature    complement(2849..2856)
                     /locus_tag="-7(1)"
                     /label=-7
                     /label=-7(1)
     misc_feature    complement(2877..2880)
                     /locus_tag="-33(1)"
                     /label=-33
                     /label=-33(1)
     misc_feature    complement(2881..2922)
                     /locus_tag="5' PcfxA(1)"
                     /label=5' PcfxA
                     /label=5' PcfxA(1)
     misc_feature    2924..2927
                     /locus_tag="Part 8 overhang"
                     /label=Part 8 overhang
     misc_feature    2971
                     /locus_tag="mutated to G"
                     /label=mutated to G
     misc_feature    2993
                     /locus_tag="mutated to C"
                     /label=mutated to C
     misc_feature    2993
                     /locus_tag="mutated to C(1)"
                     /label=mutated to C
                     /label=mutated to C(1)
     misc_feature    2994..3012
                     /locus_tag="BT-r1"
                     /label=BT-r1
     misc_feature    3081..4361
                     /locus_tag="NBU2 integrase"
                     /label=NBU2 integrase
     misc_feature    3232
                     /locus_tag="mutated to T"
                     /label=mutated to T
     misc_feature    3339
                     /locus_tag="mutated to A (silent)"
                     /label=mutated to A (silent)
     misc_feature    3736
                     /locus_tag="mutated to T(1)"
                     /label=mutated to T
                     /label=mutated to T(1)
     misc_feature    complement(4017..4019)
                     /locus_tag="mislabeled AGC(1)"
                     /label=mislabeled AGC
                     /label=mislabeled AGC(1)
     misc_feature    complement(4182..4184)
                     /locus_tag="mislabeled AGC"
                     /label=mislabeled AGC
     misc_feature    complement(4203..4205)
                     /locus_tag="mislabeled gca"
                     /label=mislabeled gca
     misc_feature    complement(4251..4253)
                     /locus_tag="mislabeled as CGC"
                     /label=mislabeled as CGC
     misc_feature    complement(4487)
                     /locus_tag="difference"
                     /label=difference
     misc_feature    4521..4533
                     /locus_tag="attN2 5' sequence"
                     /label=attN2 5' sequence
     misc_feature    4555..4568
                     /locus_tag="attN2 3' sequence"
                     /label=attN2 3' sequence
     misc_feature    4801..5535
                     /locus_tag="ermG"
                     /label=ermG
     misc_feature    6023..6884
                     /locus_tag="beta-lactamase from pBR322"
                     /label=beta-lactamase from pBR322
ORIGIN
        1 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
       61 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac
      121 cgcggtggcc ctgtacaaag aaaattcgac aaactgttat ttttctatct atttatttga
      181 attgtgagcg gataacaatt acctttgtcg gcaattgtga gcggataaca attatgatct
      241 gtcctgatga gtccgtgagg acgaaacgag taagctcgtc gacagaacga tgcgctgaat
      301 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      361 ggcaccgagt cggtgctttt ttggccggca tggtcccagc ctcctcgctg gcgccggctg
      421 ggcaacatgc ttcggcatgg cgaatgggac gcactctaat cgttatcgga gtgcttttag
      481 attactaatc aaattgcttc tactaattgc ctatcttcca gtgatggaac agcatttgaa
      541 cgcattggct gcaacaatca gccttgatct ggaagaagca atgaaagctg ctgttaagtc
      601 tccgaatcag gtattgttcc tgacaggtgt attcccatcc ggtaaacgcg gatactttgc
      661 agttgatctg actcaggaat aaattataaa ttaaggtaag aagattgtag gataagctaa
      721 tgaaatagaa aaaggatgcc gtcacacaac ttgtcggcat tcttttttgt tttattagtt
      781 gaaaatatag tgaaaaagtt gcctaaatat gtatgttaac aaattatttg tcgtaacttt
      841 gcactccaaa tctgttttta acatatggca ctagtgaaac cagtaacgtt atacgatgtc
      901 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
      961 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
     1021 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
     1081 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
     1141 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaaacg
     1201 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
     1261 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat
     1321 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg
     1381 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
     1441 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
     1501 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
     1561 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
     1621 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
     1681 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc
     1741 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
     1801 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
     1861 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     1921 gcacgacagg tttcccgact ggaaagcggg cagtgagctt tcctcggtac caaattccag
     1981 aaaagaggcc tcccgaaagg ggggcctttt ttcgttttgg tcctacttgt gcctgttcta
     2041 tttcctatgc ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2101 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac atcccaaatg
     2161 ctgttccatc actggaagat aggcaattag tagaagcaat ttgattagta atctaaaagc
     2221 actccgataa cgattagagt gcttttagat tgtttatcat taattatcaa agcaagtgca
     2281 gttcattatg ccaggatgcg ctcgcacaga cgccaacccg tgacaccgtt gatggtcacg
     2341 cggaacagca ggctgccgtc cgggttaatc agacgctcgt caataatctt attgccattc
     2401 cacagggtac cggtgacggt gatcttttta ccgtcaaaaa cggcaatgcc ttcatacgga
     2461 cggccgaaat agtcaatcat attcggggtc acaccatcaa tcaccagcgt accgtaatgc
     2521 aagataacct tgaagtggtg atcgtcaact gggtagacga ctttaaagat tttctcgatt
     2581 tgacccattt ggtcaccgct caggccttcg tacgggatga tcacatgaat atcaattttc
     2641 agaccgttct cgccggacag aacgatgcgc tgaatcggcg tcacgctcac acccaggttc
     2701 tggaacagag agcttacgcc accctgttcc aggacttgat ccaaattata acccgcggtc
     2761 tgacgccaat cgccaacaaa atcttccaga gtaaaaacca tgtttatatt atttatattt
     2821 gtttgacgag aatatcttta tttgccgaca aaggtacata actaaagttt cccacccaaa
     2881 taaatagata gaaaaataac agtttgtcga attttctttg tacccgatac ttgtgcctgt
     2941 tctatttccg aaccgaccgc ttgtatgaat ccatcaaaat tcgttttctc tatgttggat
     3001 tccttgttgc tcatattgtg atgataattt ctacaaatat agtcattggt aactatctat
     3061 gaaactgttt gatactttta tagttgatta aacttgttca tggcatttgc cttaatatca
     3121 tccgctatgt caatgtaggg tttcatagct ttgtagtcgc tgtgtcccgt ccatttcatg
     3181 accacctgtg ccgggattcc gagagccagc gcattgcaga tgaatgtcct tcttcctgca
     3241 tgggtactga gcaaagcgta tttgggtgtg acttcatcaa tacgttcatt tcccttgtag
     3301 taggtttccc gtacaggctc gttgatttct gccagttcgc ccagctcttt caggtaatcg
     3361 ttcatcttct ggttgctgat gacgggcaga gccatgtaat tctcgaaatg gatgtccttg
     3421 tatttgtcca gtatggcttt gctgtatttg ttcagttcaa tcgtcaggct gtcggcagtc
     3481 ttgactgtgg ttatttcgat gtggtcggac ttcacatcgc ttcttttcag attgcgaaca
     3541 tccgaatacc gcaaactcgt aaagcagcag aacaggaaaa catcacgcac acgttccagg
     3601 tattgcttat ccttgggtat ctggtagtct ttcagcttgt tcagttcatc ccaagtcagg
     3661 aagattactt ttttcgaggt ggttttcagt ttcggtttga acgtatcgta tgcaatgttc
     3721 tgatgatgtc ctttcctgaa gctccagcgc aggaaccatt tgaggaatcc catttgcttg
     3781 ccgatggtgc tgtttctcat atccttggtg tcacgcagga agttgacgta ttcgttcaat
     3841 ccaaactcgt tgaaatagtt gaacgttgca tcctccttga actctttgag gtggttcctc
     3901 actgctgcaa atttttcata ggtggatgcc gtccagttat tctggttacc gcactctttt
     3961 acaaactcat cgaacacctc ccaaaagctg acaggggctt cttccggctg ttcttcactg
     4021 gtgtctttca ttctcatgtt gaaagcttcc ttcaactgtt gggtcgttgg catgacctcc
     4081 tgcacctcaa attccttgaa aatattctgg atttcggcat agtatttcag caagtccgta
     4141 ttgatttcgg ctgcactttg ctttagcttg ttggtacatc cgttctttac ccgctgctta
     4201 tctacatccc atttggctac gtcaatccgg tagcccgttg taaactcgat acgttggctg
     4261 gcaaagatga cacgcatacg gatgggtacg ttctctacga ttggcacacc gttctttttc
     4321 cggctctcca atgcaaaaat gatgttgcgc ttgatattca taattgggtg cgtttgaaat
     4381 tctacaccca aatatacacc caattattga gatagcaaaa gacatttaga aacatttact
     4441 tttactctat attgtaattt acacttgatt atcagtcgtt tgcagtttta tgatattctg
     4501 tgaaagtata agttcgagag cctgtctctc cgcaaaaaac gctgaaaatc agcagattgc
     4561 aaaacaaaca ccctgtttta cacccaagaa tgtaaagtcg gctgtttttg ttttatttaa
     4621 gataatacaa ccactacata ataaaagagt agcgatatta aaagaatccg atgagaaaag
     4681 actaatattt atctatccat tcagtttgat ttttcaggac tttacatcgt cctgaaagta
     4741 tttgttggta ccggtaccga ggacgcgtaa acatttacag ttgcatgtgg cctattgttt
     4801 ttagccgtta aatattttat aactattaaa tagcgataca aattgttcga aactaatatt
     4861 gtttatatca tatattctcg catgttttaa agctttatta aattgatttt ttgtaaacag
     4921 tttttcgtac tctttgttaa cccatttcat tacaaaagtt tcatattttt ttctctcttt
     4981 aaatgccatt tttgctggct ttctttttaa tacaattaat gtgctatcca ctttaggttt
     5041 tggatggaaa taatacctag gaatttttgc taatatagaa atatctacct ctgccattaa
     5101 cagcaatgct agtgatctgt ttgtatctaa taacatttta gcaaaaccat attccactat
     5161 taaataactt attgtggctg aactttcaaa aacaattttt cgaattatat ttgtgcttat
     5221 gttgtaaggt atgctgccaa atattttata tggattgtgg ctaggaaatg taaatttcag
     5281 tatatcatca tttactattt gatagttagg ataatttaag agcttattac gagttacctc
     5341 acataattta gaatcaattt ctatcgccgt tacaaaatta catctcttta ccaatccagc
     5401 agtaaaatga cctttccctg cacctatttc aaagatgtta tctttttcat ctaaacttat
     5461 gcaattcatt attttttcta tgtgatattt tgaagtaata aaattttgac tatcttttat
     5521 atttactttg ttcattataa cctctcctta atttattgca tctcttttcg aatatttatg
     5581 ttttttgaga aaagaacgta ctcatggttc atcccgatat gcgtatcggt ctgtatatca
     5641 gcaactttct atgtgtttca actacaatag tcatctattc tcatctttct gagtccaccc
     5701 cctgcaaagc ccctctttac gacataaaaa ttcggtcgga aaaggtatgc aaaagatgtt
     5761 tctctcttta agagaaactc ttcgggatgc aaaaatatga aaataactcc aattcaccaa
     5821 attatatagc gactttttta caaaatgcta aaatttgttg atttccgtca agcaattgtt
     5881 gagcaaaaat gtcttttacg ataaaatgat acctcaatat caactgttta gcaaaacgat
     5941 atttctctta aagagagaaa cacctttttg ttcaccaatc cccgactttt aatcccgcgg
     6001 ccatgattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6061 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6121 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6181 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6241 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6301 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6361 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6421 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6481 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6541 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6601 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     6661 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6721 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6781 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6841 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6901 ctcataacgc gtcaattcga gggggatcaa ttccgtgata ggtgggctgc ccttcctggt
     6961 tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca
     7021 gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa
     7081 ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat
     7141 acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga
     7201 tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaacgg
     7261 aattgatccg gccacgatgc gtccggcgta gaggatctga agatcagcag ttcaacctgt
     7321 tgatagtacg tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa
     7381 gctctcatgt ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg
     7441 tactaagctc tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa
     7501 atcagcaact taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa
     7561 ggcttttaaa gcttttaag
//
