LOCUS       Exported               13020 bp ds-DNA     circular SYN 06-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jan 29, 2024 from SnapGene Viewer 5.2.5.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_recomb     1..100
                     /locus_tag="RP4 oriT/RSK oriV"
                     /label=RP4 oriT/RSK oriV
                     /note="RP4 oriT/RSK oriV"
     misc_feature    1..20
                     /locus_tag="NBU1-f1v1"
                     /label=NBU1-f1v1
     misc_feature    115..118
                     /locus_tag="Part 1 overhang"
                     /label=Part 1 overhang
     misc_feature    120..161
                     /locus_tag="5' PcfxA"
                     /label=5' PcfxA
     misc_feature    162..165
                     /locus_tag="-33"
                     /label=-33
     misc_feature    166..185
                     /locus_tag="O1(1)"
                     /label=O1
                     /label=O1(1)
     misc_feature    186..193
                     /locus_tag="-7"
                     /label=-7
     misc_feature    199..219
                     /locus_tag="O1"
                     /label=O1
     misc_feature    220..223
                     /locus_tag="1.5 scar"
                     /label=1.5 scar
     misc_feature    complement(224..229)
                     /locus_tag="20 bp target(1)"
                     /label=20 bp target
                     /label=20 bp target(1)
     misc_feature    230..266
                     /locus_tag="HH Ribozyme Core"
                     /label=HH Ribozyme Core
     misc_feature    267..286
                     /locus_tag="20 bp target"
                     /label=20 bp target
     misc_feature    287..368
                     /locus_tag="sgRNA"
                     /label=sgRNA
     misc_feature    367..368
                     /locus_tag="added for consistency"
                     /label=added for consistency
     misc_feature    369..436
                     /locus_tag="HDV Ribozyme"
                     /label=HDV Ribozyme
     misc_feature    437..488
                     /locus_tag="NanoLuc Terminator"
                     /label=NanoLuc Terminator
     misc_feature    551..859
                     /locus_tag="Pbt1311"
                     /label=Pbt1311
     misc_feature    798..801
                     /locus_tag="-33 site"
                     /label=-33 site
     misc_feature    820..827
                     /locus_tag="-7 site"
                     /label=-7 site
     misc_feature    860..1942
                     /locus_tag="lacI"
                     /label=lacI
     misc_feature    908..913
                     /locus_tag="YQ"
                     /label=YQ
     misc_feature    923..925
                     /locus_tag="R"
                     /label=R
     misc_feature    982..983
                     /locus_tag="end of DBD overlap"
                     /label=end of DBD overlap
     misc_feature    1822
                     /locus_tag="Change to A for Domestication"
                     /label=Change to A for Domestication
     misc_feature    1949..2009
                     /locus_tag="L3S2P21 Terminator"
                     /label=L3S2P21 Terminator
     misc_feature    2036..2136
                     /locus_tag="New Feature(2)"
                     /label=New Feature
                     /label=New Feature(2)
     regulatory      2150..2206
                     /regulatory_class="terminator"
                     /locus_tag="L3S2P55"
                     /label=L3S2P55
                     /note="L3S2P55"
     misc_feature    2168
                     /locus_tag="New Feature(1)"
                     /label=New Feature
                     /label=New Feature(1)
     regulatory      2207..2250
                     /regulatory_class="promoter"
                     /locus_tag="P1"
                     /label=P1
                     /note="P1"
     regulatory      2214..2217
                     /regulatory_class="other"
                     /locus_tag="-33 sigma factor recognitions site"
                     /label=-33 sigma factor recognitions site
                     /note="-33 sigma factor recognitions site"
     regulatory      2239..2246
                     /regulatory_class="other"
                     /locus_tag="-7 sigma factor recognitions site"
                     /label=-7 sigma factor recognitions site
                     /note="-7 sigma factor recognitions site"
     CDS             2283..6389
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="Cas9"
                     /product="Cas9"
                     /label=Cas9
                     /protein_id="QJT41641.1"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGEFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    2474
                     /locus_tag="mut to T for domestication"
                     /label=mut to T for domestication
     misc_feature    4800..4801
                     /locus_tag="H840A"
                     /label=H840A
                     /note="H840A"
     misc_difference 5593
                     /locus_tag="Gly to Glu"
                     /replace="g"
                     /label=Gly to Glu
                     /note="Gly to Glu"
     misc_feature    complement(6390..6398)
                     /locus_tag="5' PcfxA(1)"
                     /label=5' PcfxA
                     /label=5' PcfxA(1)
     misc_feature    6400..6403
                     /locus_tag="Part 8 overhang"
                     /label=Part 8 overhang
     misc_feature    6404..6426
                     /locus_tag="NBU1-attNseqFwd"
                     /label=NBU1-attNseqFwd
     misc_feature    6506
                     /locus_tag="New Feature"
                     /label=New Feature
     misc_feature    6528
                     /locus_tag="New Feature(3)"
                     /label=New Feature
                     /label=New Feature(3)
     CDS             complement(6616..7953)
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="IntN1"
                     /product="IntN1"
                     /label=IntN1
                     /note="NBU1 integrase"
                     /protein_id="QJT41633.1"
                     /translation="MKVTFIIKKAAKRYDTESMATIYVRFRNGRQLDSVAPTQLAINPN
                     LWDDKDECVKTKAVCNEEMRTHINEEIRQLKTYIEKVYQQEKEAIDKEWLKTTLDKFYH
                     PEKYFLPEEVVIKPTIGELFDEFLNKHPLSEVRKKNFRVVKRALLRYELYVRATKRGQK
                     GFILDVDLVTPDTLRDMWDFFQNEYQYYELYPSIYEAIPEKRTPQPRSKNTLIDCFSRI
                     RTFFLWCFDNKRTTNRPFDKFPIEECTYGTPYYITLEERDRIFNADLSATPQLAIQRDI
                     FIFQTLIGCRVSDLYRMTKLNVVNEAIEYIPKKTKEGNPVTVRVPLNDKAKEILERYKE
                     YEGKLLPFISEQKYNDAIKKIFKLAGVDRIVTILDPLTHNEIKRPIYEVASSHLARRTF
                     IGNIYKKVKDPNLVSALSGHKEGSKAFRRYRDIDEEMKKDLVKLLD"
     misc_feature    6622
                     /locus_tag="mut to C"
                     /label=mut to C
     misc_feature    6624
                     /locus_tag="mut to G"
                     /label=mut to G
     misc_feature    6827
                     /locus_tag="mut to T (silent)"
                     /label=mut to T (silent)
     misc_recomb     complement(7969..8298)
                     /locus_tag="attN1 region"
                     /label=attN1 region
                     /note="attN1 region"
     misc_feature    complement(8047..8067)
                     /locus_tag="NBU1-intSeqFwd"
                     /label=NBU1-intSeqFwd
     misc_feature    complement(8158..8171)
                     /locus_tag="CC Region"
                     /label=CC Region
                     /note="CC Region"
     misc_difference complement(8159)
                     /locus_tag="mutated for higher integration efficiency"
                     /replace="g"
                     /label=mutated for higher integration efficiency
                     /note="mutated for higher integration efficiency"
     misc_feature    8354..8380
                     /locus_tag="unconfirmed"
                     /label=unconfirmed
     misc_feature    8381..8400
                     /locus_tag="NBU1-intSeqRev"
                     /label=NBU1-intSeqRev
     misc_feature    8752..8753
                     /locus_tag="deleted?"
                     /label=deleted?
     misc_feature    8855..8885
                     /locus_tag="tet prom putative"
                     /label=tet prom putative
     CDS             9020..10945
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="TetQ"
                     /product="TetQ"
                     /label=TetQ
                     /protein_id="QJT41639.1"
                     /translation="MNIINLGILAHIDAGKTSVTENLLFASGATEKCGCVDNGDTITDS
                     MDIEKRRGITVRASTTSIIWNGVKCNIIDTPGHMDFIAEVERTFKMLDGAVLILSAKEG
                     IQAQTKLLFNTLQKLQIPTIIFINKIDRAGVNLERLYLDIKANLSQDVLFMQNVVDGSV
                     YPVCSQTYIKEEYKEFVCNHDDNILERYLADSEISPADYWNTIIALVAKAKVYPVLHGS
                     AMFNIGINELLDAITSFILPPASVSNRLSSYLYKIEHDPKGHKRSFLKIIDGSLRLRDV
                     VRINDSEKFIKIKNLKTINQGREINVDEVGANDIAIVEDMDDFRIGNYLGAEPCLIQGL
                     SHQHPALKSSVRPDRPEERSKVISALNTLWIEDPSLSFSINSYSDELEISLYGLTQKEI
                     IQTLLEERFSVKVHFDEIKTIYKERPVKKVNKIIQIEVPPNPYWATIGLTLEPLPLGTG
                     LQIESDISYGYLNHSFQNAVFEGIRMSCQSGLHGWEVTDLKVTFTQAEYYSPVSTPADF
                     RQLTPYVFRLALQQSGVDILEPMLYFELQIPQAASSKAITDLQKMMSEIEDISCNNEWC
                     HIKGKVPLNTSKDYASEVSSYTKGLGIFMVKPCGYQITKGGYSDNIRMNEKDKLLFMFQ
                     KSMSSK"
     misc_feature    9856
                     /locus_tag="mut to T for domestication(1)"
                     /label=mut to T for domestication
                     /label=mut to T for domestication(1)
     CDS             11436..12296
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="beta-lactamase"
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="derived from pBR322"
                     /protein_id="QJT41632.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_difference 12155
                     /locus_tag="misc_difference"
                     /replace="t"
                     /label=misc_difference
     misc_recomb     12335..13020
                     /locus_tag="RP4 oriT/RSK oriV(1)"
                     /label=RP4 oriT/RSK oriV
                     /label=RP4 oriT/RSK oriV(1)
                     /note="RP4 oriT/RSK oriV"
ORIGIN
        1 gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac
       61 acttaacggc tgacatggga attcccctcc accgcggtgt attaatgcgg ctgcccctgt
      121 acaaagaaaa ttcgacaaac tgttattttt ctatctattt atttgaattg tgagcggata
      181 acaattacct ttgtcggcaa ttgtgagcgg ataacaatta tgatgttgtc tgatgagtcc
      241 gtgaggacga aacgagtaag ctcgtcacaa cattggcacc gataacgttt tagagctaga
      301 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      361 gcttttttgg ccggcatggt cccagcctcc tcgctggcgc cggctgggca acatgcttcg
      421 gcatggcgaa tgggacgcac tctaatcgtt atcggagtgc ttttagatta ctaatcaaat
      481 tgcttctact aattgcctat cttccagtga tggaacagca tttgaacgca ttggctgcaa
      541 caatcagcct tgatctggaa gaagcaatga aagctgctgt taagtctccg aatcaggtat
      601 tgttcctgac aggtgtattc ccatccggta aacgcggata ctttgcagtt gatctgactc
      661 aggaataaat tataaattaa ggtaagaaga ttgtaggata agctaatgaa atagaaaaag
      721 gatgccgtca cacaacttgt cggcattctt ttttgtttta ttagttgaaa atatagtgaa
      781 aaagttgcct aaatatgtat gttaacaaat tatttgtcgt aactttgcac tccaaatctg
      841 tttttaacat atggcactag tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
      901 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
      961 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     1021 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     1081 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     1141 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaaacggcgg tgcacaatct
     1201 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     1261 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     1321 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     1381 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     1441 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     1501 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     1561 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     1621 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     1681 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
     1741 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     1801 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
     1861 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     1921 ccgactggaa agcgggcagt gagctttcct cggtaccaaa ttccagaaaa gaggcctccc
     1981 gaaagggggg ccttttttcg ttttggtcct acttgtgcct gttctatttc ctatgcggtt
     2041 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2101 ggataaccgt attaccgcct ttgagtgagc tgatacatcc caaatgctgc tcggtaccaa
     2161 agacgaacaa taagacgctg aaaagcgtct tttttcgttt tggtccgata aagtttggaa
     2221 gataaagcta aaagttctta tctttgcagt ccgaaataaa gacatataaa agaaaagaca
     2281 ccatggataa gaaatactca ataggcttag ctatcggcac aaatagcgtc ggatgggcgg
     2341 tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga aatacagacc
     2401 gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga gagacagcgg
     2461 aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag aatcgtattt
     2521 gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt ttctttcatc
     2581 gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat cctatttttg
     2641 gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat catctgcgaa
     2701 aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg gccttagcgc
     2761 atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct gataatagtg
     2821 atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt gaagaaaacc
     2881 ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg agtaaatcaa
     2941 gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc ttatttggga
     3001 atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt gatttggcag
     3061 aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat aatttattgg
     3121 cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta tcagatgcta
     3181 ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc ctatcagctt
     3241 caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa gctttagttc
     3301 gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa aacggatatg
     3361 caggttatat tgatggggga gctagccaag aagaatttta taaatttatc aaaccaattt
     3421 tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa gatttgctgc
     3481 gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg ggtgagctgc
     3541 atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat cgtgagaaga
     3601 ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg cgtggcaata
     3661 gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg aattttgaag
     3721 aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca aactttgata
     3781 aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag tattttacgg
     3841 tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc
     3901 tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat cgaaaagtaa
     3961 ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat agtgttgaaa
     4021 tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat ttgctaaaaa
     4081 ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta gaggatattg
     4141 ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt aaaacatatg
     4201 ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat actggttggg
     4261 gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc aaaacaatat
     4321 tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg atccatgatg
     4381 atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa ggcgatagtt
     4441 tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt attttacaga
     4501 ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca gaaaatatcg
     4561 ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc
     4621 gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt aaagagcatc
     4681 ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc caaaatggaa
     4741 gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat gatgtcgatg
     4801 ccattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc ttaacgcgtt
     4861 ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc aaaaagatga
     4921 aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag tttgataatt
     4981 taacgaaagc tgaacgtggt ggtttgagtg aacttgataa agctggtttt atcaaacgcc
     5041 aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat agtcgcatga
     5101 atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt accttaaaat
     5161 ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt gagattaaca
     5221 attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct ttgattaaga
     5281 aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat gatgttcgta
     5341 aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat ttcttttact
     5401 ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag attcgcaaac
     5461 gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa gggcgagatt
     5521 ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag
     5581 tacagacagg cgaattctcc aaggagtcaa ttttaccaaa aagaaattcg gacaagctta
     5641 ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt ccaacggtag
     5701 cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg
     5761 ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa aatccgattg
     5821 actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt aaactaccta
     5881 aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt gccggagaat
     5941 tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta tatttagcta
     6001 gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa ttgtttgtgg
     6061 agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt tctaagcgtg
     6121 ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa catagagaca
     6181 aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg aatcttggag
     6241 ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat acgtctacaa
     6301 aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat gaaacacgca
     6361 ttgatttgag tcagctagga ggtgactgat tctttgtacc cgataattgc ctatcttcca
     6421 gtgatggaac agcatttgtg cattggctgc aacaatcagc cttacttgtg cctgttctat
     6481 ttccgaaccg accgcttgta tgaatgcatc aaaattcgtt ttctctacgt tggattcctt
     6541 gttgctcata ttgtgatgat aatttctaca aatatagtca ttggtaacta tctatgaaac
     6601 tgtttgatac ttttatcagt ctaatagttt tacaaggtct ttcttcattt cttcgtcaat
     6661 atccctgtat cgtctgaaag ctttgcttcc ctccttgtgt cccgacagtg cggaaacaag
     6721 gttcgggtct ttcacttttt tatagatatt gccgataaac gtacgtcttg ccagatggct
     6781 gcttgccact tcataaatag gtcgtttgat ttcgttgtgc gtcaacgggt ctaagattgt
     6841 tacgatgcgg tcaactccag ctaatttgaa tatctttttt atggcatcat tgtacttttg
     6901 ctcggatatg aacggcaaca gttttccctc atattctttg tagcgttcaa ggatttcttt
     6961 cgctttgtcg ttaagtggaa cacgtaccgt aaccggattc ccctctttgg ttttcttggg
     7021 aatatattct atggcttcat tgaccacatt tagtttggtc attcggtaca ggtcgctcac
     7081 cctgcatcct atcagtgtct gaaatatgaa tatatccctc tgtattgcca gttgtggggt
     7141 ggcagaaagg tctgcattaa aaatcctgtc cctttcttcg agtgttatat aataaggtgt
     7201 accatatgta cactcctcta tcggaaactt gtcgaaaggt ctgtttgtgg tgcgtttgtt
     7261 atcgaagcac cacaggaaga atgtgcgtat tcttgaaaaa cagtctatca gcgtgttttt
     7321 gcttctgggc tgtggtgtcc tcttttcggg aatggcttca taaatgctcg ggtaaagttc
     7381 ataatactgg tattcgttct gaaagaaatc ccacatatcc cgaagcgtgt caggtgttac
     7441 caaatccaca tcaaggataa agcccttttg tcctctcttt gtagccctta catatagttc
     7501 ataacgcagt aaggctcttt tgacaacccg gaaatttttc tttcgtactt ccgacaaagg
     7561 gtgcttgttt agaaattcat cgaatagttc tccaatggta ggcttgataa ccacttcctc
     7621 cggcaaaaaa tatttttcag gatggtaaaa tttatcaagt gttgttttta gccattcttt
     7681 gtctattgct tccttttctt gttgatatac cttctcgata taggttttca actggcgtat
     7741 ctcttcattt atatgggtac gcatttcttc attgcagaca gctttcgttt ttacacattc
     7801 gtctttatca tcccatagat tgggattgat ggctaactga gtaggagcaa cggagtctaa
     7861 ctgccttccg tttctaaaac ggacatagat tgtagccatg gattctgtat catatcgttt
     7921 ggctgctttt ttaatgataa aggttacttt catagacttt caggttgaat tttactctgc
     7981 tgcaaatata aatattttcc ccagcatttt ccccacatct gctaaatatt ttgcaattcg
     8041 attaaacttg gattaaaatt taataggatt ataacatatt gaaatacagt gtaattgtgg
     8101 cgtttttccg catttttctt tttacccaca ttttccccac tttaattatt tcaaatgcgg
     8161 ggtctgggta caagaaagaa agctaagtat ttgatagttc aatacttagc tttttctttt
     8221 gcttgaattt tccccacatt ttccccacac gtgcaaaaaa tatagcagta agtcattatt
     8281 tcttttggtt gaacgtagag agtagcgata ttaaaagaat ccgatgagaa aagactaata
     8341 tttatctatc cattcagttt gatttttcag gactttacat cgtcctgaaa gtatttgttg
     8401 gtaccggtac cgaggacgcg taaacattta cagttgcatg tggcctattg tttaggacgc
     8461 gttatctcct taacgtacgt tttcgttcca ttggccctca aaccccgtta tatacattca
     8521 tgtccattta tgtaaaaaat cctgctgacc ttgtttatgt cttgtcagtc accatttgca
     8581 aaaccatatt tgaccctcaa agaggctgaa tttgataagc aacttgctac atactcataa
     8641 taaggagcta aatagaacac gaatgggaaa tactcaaatg ccaaactaaa gaagatattg
     8701 gccaaaataa acgctatacc gagagagaaa cttgattttt caacttccta accaacagtg
     8761 ttgttcaaac atttctactt atttgtactt accagttgaa cctacgtttc cctaataaaa
     8821 tgtctatggt aaaaagttaa aaaatcctcc tacttttgtt agatatattt ttttgtgtaa
     8881 ttttgtaatc gttatgcggc agtaataata tacatattaa tacgagttag gaatcctgta
     8941 gttctcatat gctacgagga ggtattaaaa ggtgcgtttc gacaatgcat ctattgtagt
     9001 atattattgc ttaatccaaa tgaatattat aaatttagga attcttgctc acattgatgc
     9061 aggaaaaact tccgtaaccg agaatctgct gtttgccagt ggagcaacgg aaaagtgcgg
     9121 ctgtgtggat aatggtgaca ccataacgga ctctatggat atagagaaac gtagaggaat
     9181 tactgttcgg gcttctacga catctattat ctggaatggt gtgaaatgca atatcattga
     9241 cactccggga cacatggatt ttattgcgga agtggagcgg acattcaaaa tgcttgatgg
     9301 agcagtcctc atcttatccg caaaggaagg catacaagcg cagacaaagt tgctgttcaa
     9361 tactttacag aagctgcaaa tcccgacaat tatatttatc aataagattg accgagccgg
     9421 tgtgaatttg gagcgtttgt atctggatat aaaagcaaat ctgtctcaag atgtcctgtt
     9481 tatgcaaaat gttgtcgatg gatcggttta tccggtttgc tcccaaacat atataaagga
     9541 agaatacaaa gaatttgtat gcaaccatga cgacaatata ttagaacgat atttggcgga
     9601 tagcgaaatt tcaccggctg attattggaa tacgataatc gctcttgtgg caaaagccaa
     9661 agtctatccg gtgctacatg gatcagcaat gttcaatatc ggtatcaatg agttgttgga
     9721 cgccatcact tcttttatac ttcctccggc atcggtctca aacagacttt catcttatct
     9781 ttataagata gagcatgacc ccaaaggaca taaaagaagt tttctaaaaa taattgacgg
     9841 aagtctgaga cttcgagacg ttgtaagaat caacgattcg gaaaaattca tcaagattaa
     9901 aaatctaaaa actatcaatc agggcagaga gataaatgtt gatgaagtgg gcgccaatga
     9961 tatcgcgatt gtagaggata tggatgattt tcgaatcgga aattatttag gtgctgaacc
    10021 ttgtttgatt caaggattat cgcatcagca tcccgctctc aaatcctccg tccggccaga
    10081 caggcccgaa gagagaagca aggtgatatc cgctctgaat acattgtgga ttgaagaccc
    10141 gtctttgtcc ttttccataa actcatatag tgatgaattg gaaatctcgt tatatggttt
    10201 aacccaaaag gaaatcatac agacattgct ggaagaacga ttttccgtaa aggtccattt
    10261 tgatgagatc aagactatat acaaagaacg acctgtaaaa aaggtcaata agattattca
    10321 gatcgaagtg ccgcccaacc cttattgggc cacaataggg ctgactcttg aacccttacc
    10381 gttagggaca gggttgcaaa tcgaaagtga catctcctat ggttatctga accattcttt
    10441 tcaaaatgcc gtttttgaag ggattcgtat gtcttgccaa tccgggttac atggatggga
    10501 agtgactgat ctgaaagtaa cttttactca agccgagtat tatagcccgg taagtacacc
    10561 tgctgatttc agacagctga ccccttatgt cttcaggctg gccttgcaac agtcaggtgt
    10621 ggacattctc gaaccgatgc tctattttga gttgcagata ccccaagcgg caagttccaa
    10681 agctattaca gatttgcaaa aaatgatgtc tgagattgaa gacatcagtt gcaataatga
    10741 gtggtgtcat attaaaggga aagttccatt aaatacaagt aaagactatg catcagaagt
    10801 aagttcatac actaagggct taggcatttt tatggttaag ccatgcgggt atcaaataac
    10861 aaaaggcggt tattctgata atatccgcat gaacgaaaaa gataaacttt tattcatgtt
    10921 ccaaaaatca atgtcatcaa aataatggta taacctctcc ttaatttatt gcatctcttt
    10981 tcgaatattt atgttttttg agaaaagaac gtactcatgg ttcatcccga tatgcgtatc
    11041 ggtctgtata tcagcaactt tctatgtgtt tcaactacaa tagtcatcta ttctcatctt
    11101 tctgagtcca ccccctgcaa agcccctctt tacgacataa aaattcggtc ggaaaaggta
    11161 tgcaaaagat gtttctctct ttaagagaaa ctcttcggga tgcaaaaata tgaaaataac
    11221 tccaattcac caaattatat agcgactttt ttacaaaatg ctaaaatttg ttgatttccg
    11281 tcaagcaatt gttgagcaaa aatgtctttt acgataaaat gatacctcaa tatcaactgt
    11341 ttagcaaaac gatatttctc ttaaagagag aaacaccttt ttgttcacca atccccgact
    11401 tttaatcccg cggccatgat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    11461 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    11521 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    11581 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    11641 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    11701 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    11761 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    11821 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    11881 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    11941 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    12001 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    12061 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    12121 ttgctgataa atctggagcc ggtgagcgtg ggtcacgcgg tatcattgca gcactggggc
    12181 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    12241 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    12301 cagaccaagt ttactcataa cgcgtcaatt cgagggggat caattccgtg ataggtgggc
    12361 tgcccttcct ggttggcttg gtttcatcag ccatccgctt gccctcatct gttacgccgg
    12421 cggtagccgg ccagcctcgc agagcaggat tcccgttgag caccgccagg tgcgaataag
    12481 ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct
    12541 gacgccgttg gatacaccaa ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc
    12601 gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat gaccccgaag cagggttatg
    12661 cagcggaaaa cggaattgat ccggccacga tgcgtccggc gtagaggatc tgaagatcag
    12721 cagttcaacc tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg
    12781 tttaacgtac taagctctca tgtttaacga actaaaccct catggctaac gtactaagct
    12841 ctcatggcta acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat
    12901 aaaattaata taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa
    12961 aaagaatata taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg
//
