LOCUS       pYHY1                   6431 bp ds-DNA     circular     01-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    169..757
                     /label="pBR322 ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            917..1026
                     /label="oriT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1659..2348
                     /label="RepA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /translation="MILNRKIIFPLILFLWKIIVYFVENNISQKHIKMENKKAVKLTDFQKNEENPFMKQAIEGIENHVVKKYKSNSGGDKRAVVALADTETGEVFKTSFIRQIEVDEEQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMKPKNDLIIFNRKKCLEYTKYKTDKAVYKGLAELVKAEIIARGPADNLWFINPLIVFNGDRVTFAKTYVRKKTLAAQKKEEAEKRQLSLGFDEQ*"
     CDS             3051..4154
                     /label="mobA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSISHRLESAKQRYLDTVGQKMQAKAAPIREGVIVIKQETTMQELQQFATVCKERFGIEAFQIHIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLGMERGVSSDRKHLSAMQYKTECAKEQLQELSNDISSALDKYKDAQNQLLQLQKELRSIETKKNVQKLISKASEKFYGLIGKTVNDREKDALKAKVKTLEGENEQLSDRLGKAILEKERNSTKAFKAENDKEYYRQQMDNARTTSNLLRTENQKLKAETKELKKELGKMKDLFNSEQLEALRHHFPNISKAMEEGKDLFKQITRSRGFGMGM*"
     CDS             4405..5139
                     /label="ermG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFTAGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGSIPYNISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYFHPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIYDINNISFEQFVSLFNSYKIFNG*"
     misc_feature    5408..5425
                     /label="multiple cloning sites"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(5532..6392)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
       61 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
      121 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      181 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      241 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      301 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      361 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      421 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      481 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      541 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      601 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      661 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      721 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      781 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      841 ttctgtggat aaccgtgcgg ccgcccctCC AAAGGGTTCG TGTAGACTTT CCTTGGTGTA
      901 TCCAACGGCG TCAGCCGGGC AGGATAGGTG AAGTAGGCCC ACCCGCGAGC GGGTGTTCCT
      961 TCTTCACTGT CCCTTATTCG CACCTGGCGG TGCTCAACGG GAATCCTGCT CTGCGAGGCT
     1021 GGCCGGCTAC CGCCGGCGTA ACAGATGAGG GCAAGCGGAT GGCTGATGAA ACCAAGCCAA
     1081 CCAGGAAGGG CAGCCCACCT ATCAAGGTGT ACTGCCTTCC AGACGAACGA AGAGCGATTG
     1141 AGGAAAAGGC GGCGGCGGCC GGCATGAGCC TGTCGGCCTA CCTGCTGGCC GTCGGCCAGG
     1201 GCTACAAAAT CACGGGCGTC GTGGACTATG AGCACGTCCG CGAGCTGGCC CGCATCAATG
     1261 GCGACCTGGG CCGCCTGGGC GGCCTGCTGA AACTCTGGCT CACCGACGAC CCGCGCACGG
     1321 CGCGGTTCGG TGATGCCACG ATCCTCGCCC TGCTGGCGAA GATCGAAGAG AAGCAGGACG
     1381 AGCTTGGCAA GGTCATGATG GGCGTGGTCC GCCCGAGGGC AGAGCCATGA CTTTTTTAGC
     1441 CGCTAAAACG GCCGGGGGGT GCGCGTGATT GCCAAGCACG TCCCCATaac gCATAGCGCT
     1501 CCCCGACCGA TGATTTTTAA GTGACTGATT TTGTGCTGTT TTGGGGGTAT ATTAAGAATA
     1561 AAAGAAATAG AATAAGTTAA GTACTTGATA CACAATATAG GGCATTTTCC ATATTGGAAA
     1621 TTCTCATTTT CCAATCTAGA AAATACCGAT TTCCTAATAT GATACTAAAT AGGAAAATAA
     1681 TATTTCCCTT AATATTGTTT TTATGGAAAA TAATAGTTTA CTTTGTGGAG AATAATATTT
     1741 CCCAAAAACA TATCAAAATG GAAAATAAAA AAGCAGTTAA GTTAACCGAT TTTCAAAAGA
     1801 ACGAAGAAAA TCCTTTTATG AAACAAGCTA TAGAAGGTAT TGAAAATCAT GTTGTTAAAA
     1861 AGTATAAGAG TAATAGTGGT GGCGATAAGA GAGCTGTAGT AGCTTTAGCC GACACTGAAA
     1921 CTGGAGAAGT GTTTAAGACT TCGTTTATCC GTCAAATAGA AGTAGATGAA GAACAATTCA
     1981 CTAAATTGTA TCTTTCTAAC TTTGCTGCAT TCTTTGACCT ATCACAAGCA GCTATTCGGG
     2041 TTTTTGGTTA CTTTATGACC TGCATGAAAC CCAAAAATGA TTTAATCATT TTCAATAGAA
     2101 AAAAATGCCT AGAATATACC AAATACAAAA CAGACAAAGC CGTTTATAAA GGACTTGCAG
     2161 AACTTGTAAA AGCTGAAATC ATAGCCCGAG GACCAGCCGA TAATCTTTGG TTTATTAATC
     2221 CTCTGATAGT ATTCAATGGT GACCGAGTGA CATTTGCTAA AACATACGTT CGGAAAAAGA
     2281 CTTTAGCTGC CCAAAAGAAA GAAGAAGCAG AGAAACGACA ATTATCACTT GGCTTTGATG
     2341 AACAGTAACA CTCCATTGAG TGAAGCTGCC GTTTGGTCGC TCCCCCTTTG GGGGCGGGGG
     2401 GGGATAGATA AAGTTCCTCT ATGTAAAGTT ATAATGGGGG ATGAAAGGCA AGGTTCGCTA
     2461 ACCTTACCCC GATTGTTTAT GCTTCCCAGC ATTCAATCTA TCCCCGTTTA TGGGTTTCTC
     2521 TTTCACCCTA CAAAGATAAC CTCATGGGGG AAAAATGTCC AAGAATATGG GGTAAAAACT
     2581 ATCAAGTCGG TAGAAAATAA GTATCTTTGA AGTACATTTT TAGTAGAGGT ACTGGTGTGC
     2641 CTAGGCGAAG CAGATAGGCA AAAATAAAGC CGCTGGAGAT GTCGGATGTC CGTCAATTAA
     2701 CTCCAGCGGC TTGTATTTTG GTAACTCTCC CGTATCGGTC AGCTACCGTT TGGTCAGCAA
     2761 CAAAGGTAGT ACTTTATTAT CGCAAATCCA AATGTTTTTC CCTTTATTTG TGCATTTACA
     2821 AAACATTCAC TAACTTCGCA CCTATGCAGT ATGGTGATAG CACACTAGCA CCCTAAAGAG
     2881 TGCGTGTGTA AGGGGACAAG CCCCCTTAAC CCCCTGTCAG CCGGCTGTCA CCGGCTGTTA
     2941 TCAATCTTCA AAGTCGGGTG GTTGTCAAGA TGATTTTTTT GGTTTGGTGT CGTCTTTTTT
     3001 TAAGCTGCCG CATAACGGTT TGGCGCCGAT TGTCACAAAA ATGTTAACTT ATGGGAGCAA
     3061 CAAGTATTCA TGTACAAGCA GTGAAGCCGG GGAGCGAGAT TCACAACTTT AGGGAAAAAG
     3121 AGTTGGACTA TGTTCGTCCC GAACTTAGTC ATTTGAATGA AAGCTGGGTT GGAGATAGCA
     3181 TTTCCCATCG GCTGGAGAGT GCAAAACAAA GATATCTCGA TACGGTTGGG CAGAAGATGC
     3241 AGGCTAAAGC CGCACCCATA CGAGAGGGAG TAATAGTAAT CAAACAAGAA ACCACTATGC
     3301 AGGAACTCCA GCAGTTTGCC ACGGTCTGCA AAGAACGTTT CGGTATCGAA GCGTTTCAAA
     3361 TCCATATACA CAAGGACGAA GGATACATGA ACGCAAAGCA GTGGACACCT AACCTTCATG
     3421 CCCATGTAGT TTTCGATTGG ACGCAGCCGA ATGGGAAAAG TGTGCGTTTA TCGCGTGATG
     3481 ACATGGCAGA ACTCCAAACC ATAGCATCTG AAACACTGGG CATGGAACGT GGCGTTTCTT
     3541 CTGACCGCAA ACACTTATCG GCTATGCAGT ACAAGACCGA ATGTGCGAAA GAACAGCTTC
     3601 AGGAACTATC CAACGATATA TCCAGTGCAT TAGACAAATA CAAGGACGCA CAAAACCAGC
     3661 TTCTTCAGCT TCAGAAGGAA CTACGTTCCA TTGAAACAAA GAAGAACGTC CAAAAACTCA
     3721 TTTCTAAGGC TTCAGAGAAG TTTTACGGCT TGATTGGAAA AACAGTCAAC GATAGGGAAA
     3781 AGGATGCCTT AAAAGCCAAA GTAAAGACTT TAGAGGGGGA AAATGAACAA CTATCCGATA
     3841 GACTGGGAAA GGCTATACTT GAAAAAGAGC GAAACAGCAC AAAGGCATTC AAAGCCGAGA
     3901 ATGACAAGGA GTATTATAGA CAACAAATGG ACAACGCAAG GACAACCAGT AACCTATTAA
     3961 GGACGGAGAA CCAAAAACTA AAAGCCGAAA CCAAGGAGTT GAAAAAAGAA CTTGGTAAGA
     4021 TGAAAGACTT GTTCAATTCC GAACAACTGG AGGCTTTAAG GCATCATTTC CCAAACATAT
     4081 CAAAAGCTAT GGAAGAGGGG AAAGACCTAT TCAAGCAAAT CACCAGAAGC AGAGGTTTCG
     4141 GCATGGGTAT GTAACCCCCC CCCGCCCCCA AAGGGGGAGC GACCAAACGG CAGCCTCTCT
     4201 CAATGGAGTG TTACatccCG TAAAGAGGGG CTTTGCAGGG GGTGGACTCA GAAAGATGAG
     4261 AATAGATGAC TATTGTAGTT GAAACACATA GAAAGTTGCT GATATACAGA CCGATACGCA
     4321 TATCGGGATG AACCATGAGT ACGTTCTTTT CTCAAAAAAC ATAAATATTC GAAAAGAGAT
     4381 GCAATAAATT AAGGAGAGGT TATAATGAAC AAAGTAAATA TAAAAGATAG TCAAAATTTT
     4441 ATTACTTCAA AATATCACAT AGAAAAAATA ATGAATTGCA TAAGTTTAGA TGAAAAAGAT
     4501 AACATCTTTG AAATAGGTGC AGGGAAAGGT CATTTTACTG CTGGATTGGT AAAGAGATGT
     4561 AATTTTGTAA CGGCGATAGA AATTGATTCT AAATTATGTG AGGTAACTCG TAATAAGCTC
     4621 TTAAATTATC CTAACTATCA AATAGTAAAT GATGATATAC TGAAATTTAC ATTTCCTAGC
     4681 CACAATCCAT ATAAAATATT TGGCAGCATA CCTTACAACA TAAGCACAAA TATAATTCGA
     4741 AAAATTGTTT TTGAAAGTTC AGCCACAATA AGTTATTTAA TAGTGGAATA TGGTTTTGCT
     4801 AAAATGTTAT TAGATACAAA CAGATCACTA GCATTGCTGT TAATGGCAGA GGTAGATATT
     4861 TCTATATTAG CAAAAATTCC TAGGTATTAT TTCCATCCAA AACCTAAAGT GGATAGCACA
     4921 TTAATTGTAT TAAAAAGAAA GCCAGCAAAA ATGGCATTTA AAGAGAGAAA AAAATATGAA
     4981 ACTTTTGTAA TGAAATGGGT TAACAAAGAG TACGAAAAAC TGTTTACAAA AAATCAATTT
     5041 AATAAAGCTT TAAAACATGC GAGAATATAT GATATAAACA ATATTAGTTT CGAACAATTT
     5101 GTATCGCTAT TTAATAGTTA TAAAATATTT AACGGCTAAA AACAATAGGC CACATGCAAC
     5161 TGTAAATGTT TACGCGTCCT CGGTACCAAC AAATACTTTC AGGACGATGT AAAGTCCTGA
     5221 AAAATCAAAg ctggccaatg agacgacggg gtcatcacgg ctcatcatgc gccaaacaaa
     5281 tgtgtgcaat acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc
     5341 cacctaagcc tgtgagagaa gcagacaccc gacagatcaa ggcagttaac tagtgcactg
     5401 cagtggcgtc gacggatccg aattctacag cggccgcgat tatcaaaaag gatcttcacc
     5461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5641 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
     5701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5821 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6421 atttatcagg g
//