LOCUS       pF_AA074        3008 bp DNA     circular SYN 09-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-0F
     promoter        445..668
                     /label=H1-2xTetO_v1
     misc_difference 618..636
                     /label=tet operator
     misc_difference 646..664
                     /label=tet operator
     misc_feature    671..690
                     /label=DR_v0 [EnAs]
     misc_feature    691..713
                     /label=sgCD4_v1 [EnAs]
     misc_feature    714..733
                     /label=DR_v1 [EnAs]
     misc_feature    734..756
                     /label=sgCD4_v2 [EnAs]
     misc_feature    849..860
                     /label=GG-1R
     rep_origin      complement(1320..1908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2086..2895)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgtc agaccgaacg ctgacgtcat caacccgctc caaggaatcg
      481 cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc
      541 tgtgagggac aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa
      601 tcaccataaa cgtgaaatcc ctatcagtga tagagactta taagttccct atcagtgata
      661 gagacaccgg taatttctac tcttgtagat actttgacat gttccctgag agctaatttc
      721 tactgtcgta gattgggctt accactgctg ttcccatttt ttgaattcgc tagcaagctt
      781 ggcgtaacta gatcttgaga caaatggcag tattcatcca caaaggtctt gaaaggagtg
      841 ggaattggac acgcgtcttc atcggatccc gggcccgtcg actgcagagg cctgcatgca
      901 agcttggtgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      961 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     1021 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1081 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1141 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1201 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1261 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1321 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1381 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1441 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1501 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1561 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1621 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1681 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1741 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1801 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1861 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1921 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1981 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2041 tcaagcccaa tctgaataat gttacaacca attaaccaat tctgattaga aaaactcatc
     2101 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     2161 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     2221 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     2281 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     2341 tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     2401 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     2461 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     2521 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     2581 gaatgctgtt tttccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     2641 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     2701 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     2761 gggcttccca tacaagcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     2821 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctcg acgtttcccg
     2881 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     2941 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacgggcca
     3001 gagctgca
//
