LOCUS       pF_AA102        3001 bp DNA     circular SYN 12-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-0F
     misc_feature    complement(474..484)
                     /label=BsmBI_v5
     misc_feature    complement(487..492)
                     /label=6T
     misc_feature    complement(495..505)
                     /label=BsmBI_v2
     misc_feature    complement(502..521)
                     /label=DR_v0 [EnAs]
     promoter        complement(524..772)
                     /label=U6_v1
     misc_feature    842..853
                     /label=GG-1R
     rep_origin      complement(1313..1901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2079..2888)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgtc agaaccgact cggtgccact ttttcaagac caggctaaga
      481 gacgagaaaa aacgcgtctc catctacaag agtagaaatt accggtgttt cgtcctttcc
      541 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
      601 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
      661 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt atctctctaa
      721 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcttcgaatc
      781 tagttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc
      841 tacacgcgtc ttcatcggat cccgggcccg tcgactgcag aggcctgcat gcaagcttgg
      901 tgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      961 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1021 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1081 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1921 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1981 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaagcc
     2041 caatctgaat aatgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
     2101 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     2161 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     2221 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     2281 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     2341 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     2401 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     2461 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     2521 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     2581 gtttttccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     2641 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     2701 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     2761 ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     2821 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgacgtttc ccgttgaata
     2881 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     2941 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg ccagagctgc
     3001 a
//
