LOCUS       pF_AA232        3086 bp DNA     circular SYN 09-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-0F
     promoter        444..692
                     /label=U6_v1
     misc_feature    694..713
                     /label=sgCD47_v4 [Sp]
     misc_feature    714..912
                     /label=trRNA_v14 [Sp]
     misc_difference 741..765
                     /label=PP7_v1
     misc_difference 769..793
                     /label=PP7_v2
     misc_difference 797..815
                     /label=MS2_v1
     misc_difference 819..837
                     /label=MS2_v2
     misc_feature    927..938
                     /label=GG-1R
     promoter        complement(join(1044..1050,1051..1068,1069..1074))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(1398..1986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2164..2973)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgtc agagagggcc tatttcccat gattccttca tatttgcata
      481 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
      541 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      601 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      661 ctttatatat cttgtggaaa ggacgaaaca ccggagcagg catccggcct ccggtttaag
      721 agctatgctg cgaatacgag ggagcagacg atatggcgtc gctcctctcc acgagagcat
      781 atgggctccg tggtctcgta caccatcagg gtacgtatca gacaccatca gggtctgctc
      841 gtattcgcag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
      901 ccgagtcggt gcttttttga attcgaacac gcgtcttcat cggatcccgg gcccgtcgac
      961 tgcagaggcc tgcatgcaag cttggtgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1021 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1081 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1141 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1201 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1261 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1321 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1381 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1441 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1501 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1561 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1621 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1681 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1741 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1801 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1861 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1921 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1981 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2041 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2101 taaaaatgaa gttttaaatc aagcccaatc tgaataatgt tacaaccaat taaccaattc
     2161 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     2221 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     2281 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     2341 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     2401 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     2461 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     2521 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     2581 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     2641 atattcttct aatacctgga atgctgtttt tccggggatc gcagtggtga gtaaccatgc
     2701 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     2761 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     2821 aaacaactct ggcgcatcgg gcttcccata caagcgatag attgtcgcac ctgattgccc
     2881 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
     2941 cggcctcgac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     3001 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag
     3061 attttgagac acgggccaga gctgca
//
