LOCUS       pF_AA315        3039 bp DNA     circular SYN 09-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-0F
     misc_feature    complement(474..496)
                     /label=sgPLK1_v2 [EnAs]
     misc_feature    complement(497..516)
                     /label=DR_v1 [EnAs]
     misc_feature    complement(517..539)
                     /label=sgPLK1_v1 [EnAs]
     misc_feature    complement(540..559)
                     /label=DR_v0 [EnAs]
     promoter        complement(562..810)
                     /label=U6_v1
     misc_feature    880..891
                     /label=GG-1R
     rep_origin      complement(1351..1939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2117..2926)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgtc agaaccgact cggtgccact ttttcaaaaa aaactgaggc
      481 ccgatactac ctacggatct acgacagtag aaattaatat tcgactttgg ttgccagtca
      541 tctacaagag tagaaattac cggtgtttcg tcctttccac aagatatata aagccaagaa
      601 atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa
      661 aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt
      721 tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa
      781 atatgaagga atcatgggaa ataggccctc ttcgaatcta gttgcaaaga tggataaagt
      841 tttaaacaga gaggaatctt tgcagctaat ggaccttcta cacgcgtctt catcggatcc
      901 cgggcccgtc gactgcagag gcctgcatgc aagcttggtg taatcatggt catagctgtt
      961 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1021 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1081 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1141 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1201 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1261 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2041 gatcctttta aattaaaaat gaagttttaa atcaagccca atctgaataa tgttacaacc
     2101 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     2161 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     2221 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     2281 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     2341 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     2401 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     2461 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     2521 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     2581 cacctgaatc aggatattct tctaatacct ggaatgctgt ttttccgggg atcgcagtgg
     2641 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     2701 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     2761 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg
     2821 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     2881 tggaatttaa tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat
     2941 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     3001 tgtaacatca gagattttga gacacgggcc agagctgca
//
