LOCUS       pF_AA427        2957 bp DNA     circular SYN 02-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-0F
     promoter        444..692
                     /label=U6_v1
     misc_feature    689..699
                     /label=BsmBI_v0
     misc_feature    701..706
                     /label=6T
     misc_feature    723..733
                     /label=BsmBI_v5
     misc_feature    730..789
                     /label=trRNA_v6 [Sp]
     misc_feature    798..809
                     /label=GG-1R
     rep_origin      complement(1269..1857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2035..2844)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgtc agagagggcc tatttcccat gattccttca tatttgcata
      481 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
      541 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      601 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      661 ctttatatat cttgtggaaa ggacgaaaca ccggagacgc ttttttgttt gagagctaga
      721 aacgtctcat agcaagttca aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      781 agtcggtgct tttttgaaca cgcgtcttca tcggatcccg ggcccgtcga ctgcagaggc
      841 ctgcatgcaa gcttggtgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      901 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      961 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1021 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1081 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1141 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1201 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1261 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1321 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1381 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1441 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1501 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1561 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1621 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1681 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1741 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1801 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1861 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1921 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1981 agttttaaat caagcccaat ctgaataatg ttacaaccaa ttaaccaatt ctgattagaa
     2041 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     2101 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     2161 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     2221 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     2281 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     2341 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     2401 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     2461 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     2521 taatacctgg aatgctgttt ttccggggat cgcagtggtg agtaaccatg catcatcagg
     2581 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     2641 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     2701 tggcgcatcg ggcttcccat acaagcgata gattgtcgca cctgattgcc cgacattatc
     2761 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga
     2821 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     2881 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     2941 cacgggccag agctgca
//
