LOCUS       pUC57-Kan        2670 bp DNA     circular SYN 10-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pUC57-Kan
FEATURES             Location/Qualifiers
     source          1..2670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             450..452
                     /label=Start
     CDS             456..509
                     /codon_start=1
                     /label=BPNLS_v1.1
                     /translation="KRTADGSEFESPKKKRKV"
     misc_feature    510..522
                     /label=GG-3R
     CDS             510..515
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(982..1570)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1748..2557)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3')-Ia
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagccacca tggccaaacg gacagccgac ggaagcgagt
      481 tcgagtcacc aaagaagaag cggaaagtcg gcagcagtct tcatcggatc ccgggcccgt
      541 cgactgcaga ggcctgcatg caagcttggt gtaatcatgg tcatagctgt ttcctgtgtg
      601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      721 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      781 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      841 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      901 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      961 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1021 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1081 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1141 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1201 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1261 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1321 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1381 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1441 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1501 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1561 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1621 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1681 aaattaaaaa tgaagtttta aatcaagccc aatctgaata atgttacaac caattaacca
     1741 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     1801 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     1861 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     1921 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     1981 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     2041 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     2101 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     2161 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     2221 caggatattc ttctaatacc tggaatgctg tttttccggg gatcgcagtg gtgagtaacc
     2281 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     2341 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     2401 tcagaaacaa ctctggcgca tcgggcttcc catacaagcg atagattgtc gcacctgatt
     2461 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     2521 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     2581 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
     2641 agagattttg agacacgggc cagagctgca
//
