LOCUS       pUC57-Kan        2952 bp DNA     circular SYN 10-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pUC57-Kan
FEATURES             Location/Qualifiers
     source          1..2952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             450..452
                     /label=Start
     CDS             456..509
                     /codon_start=1
                     /label=BPNLS_v1.1
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             528..767
                     /codon_start=1
                     /label=KRAB_v2.1
                     /translation="NNSQGRVTFEDVTVNFTQGEWQRLNPEQRNLYRDVMLENYSNLVS
                     VGQGETTKPDVILRLEQGKEPWLEEEEVLGSGRAE"
     misc_feature    792..804
                     /label=GG-3R
     CDS             792..797
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1264..1852)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2030..2839)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3')-Ia
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagccacca tggccaaacg gacagccgac ggaagcgagt
      481 tcgagtcacc aaagaagaag cggaaagtca gtggcggcag cagcggcaac aacagccagg
      541 gcagagtgac cttcgaggac gtgaccgtga acttcaccca gggcgagtgg cagagactga
      601 accccgagca gagaaacctg tacagagatg tgatgctgga gaactacagc aacctggtga
      661 gcgtgggcca gggcgagacc accaagcccg acgtgatcct gagactggag cagggcaagg
      721 agccctggct ggaggaggag gaggtgctgg gcagcggcag agccgagtct ggaggatcta
      781 gcggaggatc cggcagcagt cttcatcgga tcccgggccc gtcgactgca gaggcctgca
      841 tgcaagcttg gtgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
      901 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
      961 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1021 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1081 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1981 taaatcaagc ccaatctgaa taatgttaca accaattaac caattctgat tagaaaaact
     2041 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     2101 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     2161 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     2221 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     2281 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     2341 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     2401 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     2461 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     2521 cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     2581 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     2641 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     2701 catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     2761 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgacgttt
     2821 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     2881 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacgg
     2941 gccagagctg ca
//
