LOCUS       pF_AA122        3024 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             450..452
                     /label=Start
     CDS             456..602
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64_v1.1
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDM"
     CDS             603..842
                     /label=Linker_v3.1
     CDS             843..863
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40NLS_v1.6
                     /translation="PKKKRKV"
     misc_feature    864..876
                     /label=GG-3R
     CDS             864..869
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1336..1924)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2102..2911)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3')-Ia
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagccacca tggccgacgc cctggacgac ttcgacctcg
      481 atatgctggg cagcgacgct ctggatgatt tcgacctgga catgctggga agcgacgccc
      541 tggatgactt cgacctcgat atgctgggca gcgacgctct tgacgatttt gacctcgaca
      601 tgggagggcc gagctctggc gcacccccac caagtggagg gtctcctgcc gggtccccaa
      661 catctactga agaaggcacc agcgaatccg caacgcccga gtcaggccct ggtacctcca
      721 cagaaccatc tgaaggtagt gcgcctggtt ccccagctgg aagccctact tccaccgaag
      781 aaggcacgtc aaccgaacca agtgaaggat ctgcccctgg gaccagcact gaaccatctg
      841 agccaaaaaa gaagagaaag gtaggcagca gtcttcatcg gatcccgggc ccgtcgactg
      901 cagaggcctg catgcaagct tggtgtaatc atggtcatag ctgtttcctg tgtgaaattg
      961 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     1021 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1081 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1141 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1201 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1261 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1321 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1381 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1441 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1501 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1561 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1621 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1681 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1741 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1801 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1861 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1921 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2041 aaaatgaagt tttaaatcaa gcccaatctg aataatgtta caaccaatta accaattctg
     2101 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     2161 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     2221 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     2281 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     2341 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     2401 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     2461 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     2521 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     2581 attcttctaa tacctggaat gctgtttttc cggggatcgc agtggtgagt aaccatgcat
     2641 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     2701 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     2761 acaactctgg cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga
     2821 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     2881 gcctcgacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     2941 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     3001 tttgagacac gggccagagc tgca
//
